IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ RNF2 interacts with the linker region of the human P-glycoprotein. RNF2 interacts with the linker region of the human P-glycoprotein. Int. J. Oncol. (1019-6439) 2006 imex curation Rao PS., Mallya KB., Srivenugopal KS., Balaji KC., Rao US. Cancer - Interactions investigated in the context of cancer The library plamsids identified from the intial yeast two-hybrid screen were retranformed into yeast carrying the pGBKT7/linker region of P-glycoprotein, pGBKT7/NH2-terminus of the alpha subunit of ENaC or pGBKT7 vector to confirm the interaction. us.rao@ttubsc.edu 02-MAR-2006: Contacted by JYOTI. A human prostate adenocarcinoma cDNA library was used. Only protein-protein interactions spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Rao PS., Mallya KB., Srivenugopal KS., Balaji KC., Rao US. Cancer - Interactions investigated in the context of cancer The library plamsids identified from the intial yeast two-hybrid screen were retranformed into yeast carrying the pGBKT7/linker region of P-glycoprotein, pGBKT7/NH2-terminus of the alpha subunit of ENaC or pGBKT7 vector to confirm the interaction. us.rao@ttubsc.edu Accepted 2007-FEB-15 by DAVET. 02-MAR-2006: Contacted by JYOTI. A human prostate adenocarcinoma cDNA library was used. Int. J. Oncol. (1019-6439) 2006 Only protein-protein interactions imex curation RNF2 interacts with the linker region of the human P-glycoprotein. RNF2 interacts with the linker region of the human P-glycoprotein. Int. J. Oncol. (1019-6439) 2006 imex curation Rao PS., Mallya KB., Srivenugopal KS., Balaji KC., Rao US. Cancer - Interactions investigated in the context of cancer The library plamsids identified from the intial yeast two-hybrid screen were retranformed into yeast carrying the pGBKT7/linker region of P-glycoprotein, pGBKT7/NH2-terminus of the alpha subunit of ENaC or pGBKT7 vector to confirm the interaction. us.rao@ttubsc.edu 02-MAR-2006: Contacted by JYOTI. A human prostate adenocarcinoma cDNA library was used. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Rao PS., Mallya KB., Srivenugopal KS., Balaji KC., Rao US. Cancer - Interactions investigated in the context of cancer The library plamsids identified from the intial yeast two-hybrid screen were retranformed into yeast carrying the pGBKT7/linker region of P-glycoprotein, pGBKT7/NH2-terminus of the alpha subunit of ENaC or pGBKT7 vector to confirm the interaction. us.rao@ttubsc.edu Accepted 2007-FEB-15 by DAVET. 02-MAR-2006: Contacted by JYOTI. A human prostate adenocarcinoma cDNA library was used. Int. J. Oncol. (1019-6439) 2006 Only protein-protein interactions imex curation mdr1_human ATP-dependent translocase ABCB1 ABCB1 MDR1 PGY1 ATP-binding cassette sub-family B member 1 P-glycoprotein 1 Multidrug resistance protein 1 Phospholipid transporter ABCB1 protein protein human Homo sapiens Human MDLEGDRNGGAKKKNFFKLNNKSEKDKKEKKPTVSVFSMFRYSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITNRSDINDTGFFMNLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLSTKEALDESIPPVSFWRIMKLNLTEWPYFVVGVFCAIINGGLQPAFAIIFSKIIGVFTRIDDPETKRQNSNLFSLLFLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAHKLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAGTKRQ F0230744AB288C73 ring2_human E3 ubiquitin-protein ligase RING2 RING finger protein 2 RING finger protein 1B RING finger protein BAP-1 Protein DinG Huntingtin-interacting protein 2-interacting protein 3 RNF2 BAP1 DING HIPI3 RING1B RING-type E3 ubiquitin transferase RING2 protein protein human Homo sapiens Human MSQAVQTNGTQPLSKTWELSLYELQRTPQEAITDGLEIVVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCADCIITALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSRDEYEAHQERVLARINKHNNQQALSHSIEEGLKIQAMNRLQRGKKQQIENGSGAEDNGDSSHCSNASTHSNQEAGPSNKRTKTSDDSGLELDNNNAAMAIDPVMDGASEIELVFRPHPTLMEKDDSAQTRYIKTSGNATVDHLSKYLAVRLALEELRSKGESNQMNLDTASEKQYTIYIATASGQFTVLNGSFSLELVSEKYWKVNKPMELYYAPTKEHK DIP protein Q99496 original sequence version: 95 DIP protein Q99496 original sequence version: 94 DIP protein Q99496 original sequence version: 96 DIP protein Q99496 original sequence version: 125 DIP protein Q99496 original sequence version: 142 DIP protein Q99496 original sequence version: 159 DIP protein Q99496 original sequence version: 129 DIP protein Q99496 original sequence version: 99 DIP protein Q99496 original sequence version: 118 90EA546E1F4DB7EC abcb1-rnf2 2 3 unspecified role unspecified role bait bait region Linker region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey physical association physical association The results of this study indicated that only RNF2 cDNA is a true positive interactor with the linker region. abcb1-rnf2-1 1 4 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 3 unspecified role unspecified role bait bait physical association physical association 2 B These results suggest that RNF2 interacts with Pgp and the interactions between these proteins were not affected by the NP-40 and Triton X-100 detergents. The detergents NP-40 and Triton X-100 did not disrupt this interaction.