IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Physical and functional interaction of Fyn tyrosine kinase with a brain-enriched Rho GTPase-activating protein TCGAP. Physical and functional interaction of Fyn tyrosine kinase with a brain-enriched Rho GTPase-activating protein TCGAP. J. Biol. Chem. (0021-9258) 2006 imex curation Liu H., Nakazawa T., Tezuka T., Yamamoto T. tyamamot@ims.u-tokyo.ac.jp 10-APR-2007: Contacted by JYOTI. Human fetal brain cDNA Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Liu H., Nakazawa T., Tezuka T., Yamamoto T. tyamamot@ims.u-tokyo.ac.jp Accepted 2007-APR-20 by JYOTI. 10-APR-2007: Contacted by JYOTI. Human fetal brain cDNA J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Physical and functional interaction of Fyn tyrosine kinase with a brain-enriched Rho GTPase-activating protein TCGAP. Physical and functional interaction of Fyn tyrosine kinase with a brain-enriched Rho GTPase-activating protein TCGAP. J. Biol. Chem. (0021-9258) 2006 imex curation Liu H., Nakazawa T., Tezuka T., Yamamoto T. tyamamot@ims.u-tokyo.ac.jp 10-APR-2007: Contacted by JYOTI. Human fetal brain cDNA Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Liu H., Nakazawa T., Tezuka T., Yamamoto T. tyamamot@ims.u-tokyo.ac.jp Accepted 2007-APR-20 by JYOTI. 10-APR-2007: Contacted by JYOTI. Human fetal brain cDNA J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Physical and functional interaction of Fyn tyrosine kinase with a brain-enriched Rho GTPase-activating protein TCGAP. Physical and functional interaction of Fyn tyrosine kinase with a brain-enriched Rho GTPase-activating protein TCGAP. J. Biol. Chem. (0021-9258) 2006 imex curation Liu H., Nakazawa T., Tezuka T., Yamamoto T. tyamamot@ims.u-tokyo.ac.jp 10-APR-2007: Contacted by JYOTI. Human fetal brain cDNA Only protein-protein interactions mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Liu H., Nakazawa T., Tezuka T., Yamamoto T. tyamamot@ims.u-tokyo.ac.jp Accepted 2007-APR-20 by JYOTI. 10-APR-2007: Contacted by JYOTI. Human fetal brain cDNA J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation fyn_human Tyrosine-protein kinase Fyn p59-Fyn Proto-oncogene c-Fyn Src-like kinase FYN Proto-oncogene Syn protein protein human Homo sapiens Human MGCVQCKDKEATKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSERAAGLCCRLVVPCHKGMPRLTDLSVKTKDVWEIPRESLQLIKRLGNGQFGEVWMGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQSFLEDYFTATEPQYQPGENL DIP protein P06241 original sequence version: 195 DIP protein P06241 original sequence version: 147 DIP protein P06241 original sequence version: 164 4A1E443A4B5A0977 rhg33_human Rho GTPase-activating protein 33 ARHGAP33 TCGAP SNX26 Rho-type GTPase-activating protein 33 Tc10/CDC42 GTPase-activating protein Sorting nexin-26 protein protein human Homo sapiens Human MVARSTDSLDGPGEGSVQPLPTAGGPSVKGKPGKRLSAPRGPFPRLADCAHFHYENVDFGHIQLLLSPDREGPSLSGENELVFGVQVTCQGRSWPVLRSYDDFRSLDAHLHRCIFDRRFSCLPELPPPPEGARAAQMLVPLLLQYLETLSGLVDSNLNCGPVLTWMELDNHGRRLLLSEEASLNIPAVAAAHVIKRYTAQAPDELSFEVGDIVSVIDMPPTEDRSWWRGKRGFQVGFFPSECVELFTERPGPGLKADADGPPCGIPAPQGISSLTSAVPRPRGKLAGLLRTFMRSRPSRQRLRQRGILRQRVFGCDLGEHLSNSGQDVPQVLRCCSEFIEAHGVVDGIYRLSGVSSNIQRLRHEFDSERIPELSGPAFLQDIHSVSSLCKLYFRELPNPLLTYQLYGKFSEAMSVPGEEERLVRVHDVIQQLPPPHYRTLEYLLRHLARMARHSANTSMHARNLAIVWAPNLLRSMELESVGMGGAAAFREVRVQSVVVEFLLTHVDVLFSDTFTSAGLDPAGRCLLPRPKSLAGSCPSTRLLTLEEAQARTQGRLGTPTEPTTPKAPASPAERRKGERGEKQRKPGGSSWKTFFALGRGPSVPRKKPLPWLGGTRAPPQPSGSRPDTVTLRSAKSEESLSSQASGAGLQRLHRLRRPHSSSDAFPVGPAPAGSCESLSSSSSSESSSSESSSSSSESSAAGLGALSGSPSHRTSAWLDDGDELDFSPPRCLEGLRGLDFDPLTFRCSSPTPGDPAPPASPAPPAPASAFPPRVTPQAISPRGPTSPASPAALDISEPLAVSVPPAVLELLGAGGAPASATPTPALSPGRSLRPHLIPLLLRGAEAPLTDACQQEMCSKLRGAQGPLGPDMESPLPPPPLSLLRPGGAPPPPPKNPARLMALALAERAQQVAEQQSQQECGGTPPASQSPFHRSLSLEVGGEPLGTSGSGPPPNSLAHPGAWVPGPPPYLPRQQSDGSLLRSQRPMGTSRRGLRGPAQVSAQLRAGGGGRDAPEAAAQSPCSVPSQVPTPGFFSPAPRECLPPFLGVPKPGLYPLGPPSFQPSSPAPVWRSSLGPPAPLDRGENLYYEIGASEGSPYSGPTRSWSPFRSMPPDRLNASYGMLGQSPPLHRSPDFLLSYPPAPSCFPPDHLGYSAPQHPARRPTPPEPLYVNLALGPRGPSPASSSSSSPPAHPRSRSDPGPPVPRLPQKQRAPWGPRTPHRVPGPWGPPEPLLLYRAAPPAYGRGGELHRGSLYRNGGQRGEGAGPPPPYPTPSWSLHSEGQTRSYC 802900F18B640DC0 fyn_mouse Tyrosine-protein kinase Fyn p59-Fyn Fyn Proto-oncogene c-Fyn protein protein mouse Mus musculus Mouse MGCVQCKDKEAAKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSERAAGLCCRLVVPCHKGMPRLTDLSVKTKDVWEIPRESLQLIKRLGNGQFGEVWMGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQGFLEDYFTATEPQYQPGENL 99558702596DAEE0 rhg33_mouse Rho GTPase-activating protein 33 Arhgap33 Tcgap Snx26 Rho-type GTPase-activating protein 33 Tc10/CDC42 GTPase-activating protein Sorting nexin-26 protein protein mouse Mus musculus Mouse MLQAQKQSDPILPWGASWAGRGQTLRARSTDSLDGPGEGSVQPVPTTGGPGTKGKPGKRLSAPRGPFPRLADCAHFHYENVDFGHIQLLLSPEREGPSLSGENELVFGVQVTCQGRSWPVLRSYDDFRSLDAHLHRCIFDRRFSCLPELPPPPEGTRAAQMLVPLLLQYLETLSGLVDSNLNCGPVLTWMELDNHGRRLLLSEEASLNIPAVAAAHVVKRYTAQAPDELSFEVGDIVSVIDMPPTEDRSWWRGKRGFQVGFFPSECVELFTERPGPGLKADADSPLCGIPAPQGISSLTSAVPRPRGKLAGLLRTFMRSRPSRQRLRQRGILRQRVFGCDLGEHLSNSGQDVPQVLRCCSEFIEAHGVVDGIYRLSGVSSNIQRLRHEFDSERIPELSGPAFLQDIHSVSSLCKLYFRELPNPLLTYQLYGKFSEAMSVPGEEERLVRVHDVIQQLPPPHYRTLEYLLRHLARMARHSANTSMHARNLAIVWAPNLLRSMELESVGLGGAAAFREVRVQSVVVEFLLTHVEVLFSDTFTSAGLDPAGRCLLPRPKSLAGSSPSTRLLTLEEAQARTQGRLGTPTEPTTPKTPASPVERRKRERAEKQRKPGGSSWKTFFALGRGPSIPRKKPLPWLGGSRAPPQPSGSRPDTVTLRSAKSEESLSSQASGAGLQRLHRLRRPHSSSDAFPVGPAPAGSCESLSSSSSSSSSSSSSSSSESSAGGLGPLSGSPSHRTSAWLDDGDDLDFSPPRCLEGLRGLDFDPLTFRCSSPTPGDPAPPASPAPPASASAFPPRATPQALSPHGPTKPASPTALDISEPLAVSVPPAVLELLGAGGTPASATPTPALSPHLIPLLLRGAEAQLSDTCQQEISSKLAPTRGAPGQQSPGGMDSPLLPPPLPLLRPGGAPPPPPKNPARLMALALAERAQQVAEQQSQQEQGGTPPAPHSPFRRSLSLEVGGEPVGTSGSGIHPPSLAHPGAWAPGPPPYLPRQQSDGSLVRSQRPLGTSRRSPRGPSQVSAHLRASGAYRDAPEMAAQSPCSVPSQGSNPSFFSTPRECLPPFLGVPKQGLYSLGPPSFPPSSPAPVWRNSLGAPSALDRGENLYYEIGVGEGTSYSGPSRSWSPFRSMPPDRHNASYGMLGQSPPLHRSPDFLLSYPPPPSCFPPEHLTHSVSQRLARRPTRPEPLYVNLALGPRGPSPASSSSSSPPAHPRSRSDPGPPVPRLPQKQRAPWGPHTPHRVPGPWGSPEPFLLYRPAPPSYGRGGEVRGSLYRNGGHRGEGAGPPPPYPTPSWSLHSEGQTRSYC AA8723BDF4A3CBB6 fyn-snx26-1 2 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role bait bait physical association physical association To identify proteins that interact with Fyn in the brain, we carried out yeast two-hybrid screening with full-length Fyn as bait. fyn-snx26-2 1 4 unspecified role unspecified role prey prey P06241:p.Arg176Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R K Wed May 02 19:44:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg176lys' Tue Jun 30 19:13:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg176Lys' 5 unspecified role unspecified role bait bait physical association physical association As shown in Fig. 1A, Fyn was co-immunoprecipitated with TCGAP. 1A, 1B and 3A SNX26 (O145559) appeared to tyrosine phosphorylated by FYN. This was inhibited by expression of the kinase-dead lys299met FYN mutant (Fig. 1C) or the tyr406phe mutant of SNX26 (Fig 3A). fyn-snx26-3 3 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Moreover, Fyn was co-immunoprecipitated with TCGAP from brain lysates of wild-type mice (Fig. 1D). 1D Snx26 appeared to be tyrosine phosphorylated in wild type cells but not in cells from Fyn knockout mice