UniProt UniProt http://www.uniprot.org A Mediator subunit, MDT-15, integrates regulation of fatty acid metabolism by NHR-49-dependent and -independent pathways in C. elegans. A Mediator subunit, MDT-15, integrates regulation of fatty acid metabolism by NHR-49-dependent and -independent pathways in C. elegans. Genes Dev. (0890-9369) 2006 imex curation Taubert S., Van Gilst MR., Hansen M., Yamamoto KR. revisit interaction EBI-1534334 when boundaries of nhr-49 LBD available (requested sequence from authors) yamamoto@cmp.ucsf.edu 13-Nov-2007: Contacted by JYOTI. A mixed stage c.elegans cDNA library was used. davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Taubert S., Van Gilst MR., Hansen M., Yamamoto KR. revisit interaction EBI-1534334 when boundaries of nhr-49 LBD available (requested sequence from authors) yamamoto@cmp.ucsf.edu Accepted 2007-NOV-3 by DAVET. 13-Nov-2007: Contacted by JYOTI. A mixed stage c.elegans cDNA library was used. Genes Dev. (0890-9369) 2006 davet Only protein-protein interactions imex curation A Mediator subunit, MDT-15, integrates regulation of fatty acid metabolism by NHR-49-dependent and -independent pathways in C. elegans. A Mediator subunit, MDT-15, integrates regulation of fatty acid metabolism by NHR-49-dependent and -independent pathways in C. elegans. Genes Dev. (0890-9369) 2006 imex curation Taubert S., Van Gilst MR., Hansen M., Yamamoto KR. revisit interaction EBI-1534334 when boundaries of nhr-49 LBD available (requested sequence from authors) yamamoto@cmp.ucsf.edu 13-Nov-2007: Contacted by JYOTI. A mixed stage c.elegans cDNA library was used. davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Taubert S., Van Gilst MR., Hansen M., Yamamoto KR. revisit interaction EBI-1534334 when boundaries of nhr-49 LBD available (requested sequence from authors) yamamoto@cmp.ucsf.edu Accepted 2007-NOV-3 by DAVET. A mixed stage c.elegans cDNA library was used. Genes Dev. (0890-9369) 2006 davet Only protein-protein interactions imex curation med15_caeel Mediator of RNA polymerase II transcription subunit 15 R12B2.5 Mediator complex subunit 15 mdt-15 protein protein caeel Caenorhabditis elegans MSEEDWPSPKFREHVIQRLEPELARNRQNAPNLPVPGDARQVEEYVFAKCMSKDEYMRTIAKVINAINCNSKSAAVPSVLQPSQFHSPPCTTAALLGNTPAGGTPGYRAPVPPDPQPTSAQARNPPVTVATTQASTTPSAPNPPGGLPAPSASATAAVAAAVASFPSPDTSIRPGGQITPGSQAPGGGPTPAPNVPFPNGSSQMNGGPAMGQPPPQMGAPNMGGPPNGYGGYGMMNGPPGSGAPMGGNPYNQQIKKDMDQARPWDPSAHMYQQQPQWGAMPPQQPHGYPGRPMNGQGATPTGPSSVLESLINQPQQYPGHHNQMGPPGDRNVAAQRAAAQQQQQQQQQQQQQRPGMVPNQGMMSSEDQTVYSAKLRNMRGSCDSLRTRARQCRHEGNHEAAHKLEVMLSVLEGKRVVSLEYLNHLEMWIARKQDFLNIAPMSQNQNHMGMNDPMMNGEHAMLGNGQVPNPYGGHPGYGHQQYMGPPPPHMQMHQPPMWHQQQHQQQQRMMPQDHMMMQGGGGPVHGMYRGDMGHDPMTSPVNNHRHAPYPNPAAMRNNMRMPNGPGPIGRDRNSMSGSSMSGPSSGAPSMNPMGTPNQKMGTPGSMGGMSGLDDLNYDDFLPNPTPIDALQPTLHVGQNSMNAGPPVQRSNLNETARKELQILDARFEIDPNHQRHDANHIIVVCKIRNQQFPPLRLVVPTTYPAGNVTVDRAVIDLDAYLYDDLQNSVYERLSRPGLSSITDYLNAWEEQVNQYQNQTSGGLDVAFNVGNDFFYDNLNL DIP protein Q21955 original sequence version: 89 4259A33584603D0A nhr64_caeel Nuclear hormone receptor family member nhr-64 nhr-64 C45E1.1 protein protein caeel Caenorhabditis elegans MTLEEKEEVSTSTSQSPQSSSFENVFCAICGDRATGKHYGAMSCDGCKGFFRRTIRKRHSYVCRFGEKCQVDKAKRNSCRKCRFDVCLRKGMRRDAVQTERDRIRPANPLSNGSNGGIVPDDPLLDTLIRAEASTRGLRTTVITKTAEARKQATTNDVTDSMNQQLTLMVEWAKVLEGFQRVDNITQVALLRHFSAQHLVMCAAFRSIHLSDAVWLTNETCLHKDSPKIPDMNRVAERIIDQVTNPMRSLHMNEIEYIALKAIAFFDPLAKGITSESYSDVEEMRQRILESFERHVRYVSPYKDMPLRFANLLLLLPPMLAISRDLVEDVQLAKLFGLASIDNLMLELMLPNEGKNTTDKTSPPIMCHQ 492D12EE85CEFD7E nhr49_caeel Nuclear hormone receptor family member nhr-49 nhr-49 K10C3.6 protein protein caeel Caenorhabditis elegans MDYFLDASKHIQLNQIDEESSDDFMDLVDPLAEPCAVCGDKSTGTHYGVISCNGCKGFFRRTVLRDQKFTCRFNKRCVIDKNFRCACRYCRFQKCVQVGMKREAIQFERDPVGSPTSGASLNGTPFKKDRSPGYENGNSNGVGSNGMGQENMRTVPQSSSVIDALMEMEARVNQEMCNRYRRSQIFANGSGGSNGNDTDIQQGSDSGASAFAPPNRPCTTEVDLNEISRTTLLLMVEWAKTINPFMDLSMEDKIILLKNYAPQHLILMPAFRSPDTTRVCLFNNTYMTRDNNTDLNGFAAFKTSNITPRVLDEIVWPMRQLQMREQEFVCLKALAFLHPEAKGLSNSSQIMIRDARNRVLKALYAFILDQMPDDAPTRYGNILLLAPALKALTQLLIENMTLTKFFGLAEVDSLLSEFILDDINDHSTAPVSLQQHLSSPTTLPTNGVSPLNPAGSVGSVSSVSGITPTGMLSATLAAPLAIHPLQSQDSILNSEQNNHML BC69815507B2C811 nhr49-mdt15-1 2 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Fig 1A nhr64-mdt15-1 1 4 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association Fig 1A mdt15-nhr49-1 1 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 1B nhr49-mdt15-2 1 5 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association Fig 1A