UniProt UniProt http://www.uniprot.org Glc7/protein phosphatase 1 regulatory subunits can oppose the Ipl1/aurora protein kinase by redistributing Glc7. Glc7/protein phosphatase 1 regulatory subunits can oppose the Ipl1/aurora protein kinase by redistributing Glc7. Mol. Cell. Biol. (0270-7306) 2006 Pinsky BA., Kotwaliwale CV., Tatsutani SY., Breed CA., Biggins S. sbiggins@fhcrc.org 07-Aug-2007: Contacted by JYOTI. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Pinsky BA., Kotwaliwale CV., Tatsutani SY., Breed CA., Biggins S. sbiggins@fhcrc.org Accepted 2007-AUG-20 by ORCHARD. Mol. Cell. Biol. (0270-7306) 2006 orchard sol1_yeast 6-phosphogluconolactonase-like protein 1 SOL1 N3291 YNR034W protein protein yeast Saccharomyces cerevisiae Baker's yeast MTTTVPKVFAFHEFAGVAEAVADHVIHAQNSALKKGKVSRSTQMSGTSLNGNGNTESKTMERVNSVRSNASSRGGSEDGATKKLKKEKERRFKIALSGGSLIQVLHEGLLKRDDVQWGKWDIYFADERLVPFSSSESNYGLAKRKIFDLIDTEKYGTPKIYHIDESLINDPQECADNYEKILIKGFAGRDSVKLPMFDLFLLGCAPDGHIASLFPNFQENLRENLAWVIPVENAPSGPSNRISLTIPVICHSHRVTFVVEGATKAPVIKTIMERPEKGLPSSIVNEGAAGRVSWFVDDDALKDVFVIKKKYKFYDDENLTE 177421932ED38F9C pp12_yeast Serine/threonine-protein phosphatase PP1-2 GLC7 CID1 DIS2 YER133W protein protein yeast Saccharomyces cerevisiae Baker's yeast MDSQPVDVDNIIDRLLEVRGSKPGQQVDLEENEIRYLCSKARSIFIKQPILLELEAPIKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFILRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIIDEKIFCMHGGLSPDLNSMEQIRRVMRPTDIPDVGLLCDLLWSDPDKDIVGWSENDRGVSFTFGPDVVNRFLQKQDMELICRAHQVVEDGYEFFSKRQLVTLFSAPNYCGEFDNAGAMMSVDESLLCSFQILKPAQKSLPRQAGGRKKK 71B20E3A4ED93235 71B20E3A4ED93235 pex31_yeast Peroxisomal membrane protein PEX31 Peroxin-31 PEX31 YGR004W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEINNENLEPTSSTVAESTESKNKHIRSALRKRRGKLSAQTYEEDQEAILSSPLLTSTPKTVSRSLVRLYPYLIVVDNFLSIITWSNDNVSANLLGIFLFTVCVLYFGFITRYFGHLMIVGIIWVYLLIDKHVQETMASCPSLDDIIHVMDRVSMKSSAVLSPITILSAQDVRRLLFTIAFLSPVYIFLTVFVLSPNYLMLIGGLYVLTYHSKLIRRMRRYLWKFRVVRLLVFFITGLDLGGPDNNRRLFASVNKKIRSFVWNEVGNTSNTKKTVLFKVALFENQRRWLGIGWTSTMLSYERASWTDEFLNTSPSPEVFTLPEEQSGMAWEWHDKDWMLDLTNDGIIQLPASAAKTKVKPGADEGFIYYDNTWNNPSATDTYKKYTRRRRWIRTATVTTTYDDEPTVEKATPNSHALKSEENNRVRKRKVSFSTANEVHIIPSSDSSKLIQISDVSMSPSL 3F875D40748AB6C3 gip4_yeast GLC7-interacting protein 4 YAL031C GIP4 FUN21 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVDVQKRKKLLAKAAASASIPAIKGSVPLDSYDIKIIQYKNALYKLNELNRLLNVLVPHLKKKRDNDESYKIIPLVNFILSLCEGPIFNVSPVLAKRYHLLCRFQLIKLSEVQQRLSTNFIDVEGWMFPEEVPLDHYKSCIYNNSLQWKILNSLSCIAQNAIKIYNAKLRQILLERDAYKARSLPFDTSIIEDLLNPVEMTLILDLAVLINDPVRDKSTHSFYKLQWQVMEKLNSCVHSKIFPILRTYYNQLQKFSETRPTSLSNLQKDLPHWEWTLHRIYTFHLRVFSVLCVIISFSRQIFLPNKQHFLDIKTRLSSENVYHYDLIICELMALLSPECDDVTALFELQENLKFWTQTARTDNNSSRTPIFHLQPGLVVELFNNHICKIIPKLRSIMGLLSNWMDCWKYIEKNYKTFDETNDLRENLKEKLERDKALYLEVKNAKSKLKKKPSITKLPASSSPSPSPTSSASPSRQASLESIRTRARAHLASNSSRSPSVSPVRTTFNNKNAETKKSVVSPEKRKLINGRRPRSSSLQSYTNKQQTSYLNSTRHPSIAPPSKLNNQRSNSLQSSTMTLNQKIVQDTVRHLMNKSASTPNPSASSSLAPSPKVSSINNTSSGKSSSTLIANSSDTLAIETLTLDPESNSSELSIKRVRFAGVPPMTEAENPKPTKVGWYKKPAVLHYPPIPASAMIKPLQHKSKYNTLRQEEGFTFRKSLRDGLEWENGESGSETTMMPFGIEIKESTGHRIASKIRSKLR 95C5C7DAD4B5D2C8 glc7-fun21 1 5 unspecified role unspecified role bait bait c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 3 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 4B We generated strains coexpressing endogenous COOH-terminal fusions of Glc7-HA3 with Pex31-myc13, Sol1-myc13, and Fun21-myc13 and found that all three proteins immunoprecipitated Glc7 (Fig. 4B). glc7-sol1 1 2 unspecified role unspecified role bait bait c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 3 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 4B We generated strains coexpressing endogenous COOH-terminal fusions of Glc7-HA3 with Pex31-myc13, Sol1-myc13, and Fun21-myc13 and found that all three proteins immunoprecipitated Glc7 (Fig. 4B). glc7-pex31 1 3 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role bait bait c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 4B We generated strains coexpressing endogenous COOH-terminal fusions of Glc7-HA3 with Pex31-myc13, Sol1-myc13, and Fun21-myc13 and found that all three proteins immunoprecipitated Glc7 (Fig. 4B).