IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The elucidation of novel SH2 binding sites on PLD2. The elucidation of novel SH2 binding sites on PLD2. Oncogene (0950-9232) 2006 imex curation Di Fulvio M., Lehman N., Lin X., Lopez I., Gomez-Cambronero J. julian.cambronero@wright.edu 07-Sept-2007: Contacted by JYOTI. 2007-07-17: Julian Gomez-Cambronero, Cell Biology and Physiology, Wright State University, School of Medicine, Dayton, OH, 45435, USA orchard Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Di Fulvio M., Lehman N., Lin X., Lopez I., Gomez-Cambronero J. julian.cambronero@wright.edu Accepted 2007-SEP-31 by ORCHARD. 07-Sept-2007: Contacted by JYOTI. 2007-07-17: Julian Gomez-Cambronero, Cell Biology and Physiology, Wright State University, School of Medicine, Dayton, OH, 45435, USA Oncogene (0950-9232) 2006 orchard Only protein-protein interactions imex curation The elucidation of novel SH2 binding sites on PLD2. The elucidation of novel SH2 binding sites on PLD2. Oncogene (0950-9232) 2006 imex curation Di Fulvio M., Lehman N., Lin X., Lopez I., Gomez-Cambronero J. julian.cambronero@wright.edu 07-Sept-2007: Contacted by JYOTI. 2007-07-17: Julian Gomez-Cambronero, Cell Biology and Physiology, Wright State University, School of Medicine, Dayton, OH, 45435, USA orchard Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Di Fulvio M., Lehman N., Lin X., Lopez I., Gomez-Cambronero J. julian.cambronero@wright.edu Accepted 2007-SEP-31 by ORCHARD. 07-Sept-2007: Contacted by JYOTI. 2007-07-17: Julian Gomez-Cambronero, Cell Biology and Physiology, Wright State University, School of Medicine, Dayton, OH, 45435, USA Oncogene (0950-9232) 2006 orchard Only protein-protein interactions imex curation The elucidation of novel SH2 binding sites on PLD2. The elucidation of novel SH2 binding sites on PLD2. Oncogene (0950-9232) 2006 imex curation Di Fulvio M., Lehman N., Lin X., Lopez I., Gomez-Cambronero J. julian.cambronero@wright.edu 07-Sept-2007: Contacted by JYOTI. 2007-07-17: Julian Gomez-Cambronero, Cell Biology and Physiology, Wright State University, School of Medicine, Dayton, OH, 45435, USA orchard Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Di Fulvio M., Lehman N., Lin X., Lopez I., Gomez-Cambronero J. PLD2 activity cannot be ascertained since the precipitated protein complex not analysed. julian.cambronero@wright.edu Accepted 2007-SEP-31 by ORCHARD. 07-Sept-2007: Contacted by JYOTI. 2007-07-17: Julian Gomez-Cambronero, Cell Biology and Physiology, Wright State University, School of Medicine, Dayton, OH, 45435, USA Oncogene (0950-9232) 2006 orchard Only protein-protein interactions imex curation pld2_human Phospholipase D2 Choline phosphatase 2 Phosphatidylcholine-hydrolyzing phospholipase D2 PLD1C PLD2 protein protein human Homo sapiens Human MTATPESLFPTGDELDSSQLQMESDEVDTLKEGEDPADRMHPFLAIYELQSLKVHPLVFAPGVPVTAQVVGTERYTSGSKVGTCTLYSVRLTHGDFSWTTKKKYRHFQELHRDLLRHKVLMSLLPLARFAVAYSPARDAGNREMPSLPRAGPEGSTRHAASKQKYLENYLNRLLTMSFYRNYHAMTEFLEVSQLSFIPDLGRKGLEGMIRKRSGGHRVPGLTCCGRDQVCYRWSKRWLVVKDSFLLYMCLETGAISFVQLFDPGFEVQVGKRSTEARHGVRIDTSHRSLILKCSSYRQARWWAQEITELAQGPGRDFLQLHRHDSYAPPRPGTLARWFVNGAGYFAAVADAILRAQEEIFITDWWLSPEVYLKRPAHSDDWRLDIMLKRKAEEGVRVSILLFKEVELALGINSGYSKRALMLLHPNIKVMRHPDQVTLWAHHEKLLVVDQVVAFLGGLDLAYGRWDDLHYRLTDLGDSSESAASQPPTPRPDSPATPDLSHNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGLPARDLARHFIQRWNFTKTTKAKYKTPTYPYLLPKSTSTANQLPFTLPGGQCTTVQVLRSVDRWSAGTLENSILNAYLHTIRESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHKQGWCYRVYVLLPLLPGFEGDISTGGGNSIQAILHFTYRTLCRGEYSILHRLKAAMGTAWRDYISICGLRTHGELGGHPVSELIYIHSKVLIADDRTVIIGSANINDRSLLGKRDSELAVLIEDTETEPSLMNGAEYQAGRFALSLRKHCFGVILGANTRPDLDLRDPICDDFFQLWQDMAESNANIYEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQGHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT DIP protein O14939 original sequence version: 128 F25F6B73B45F57ED grb2_cerae_protein Cercopithecus aethiops Grb2 protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey An amino acid sequence which is not represented in UniProt Similar to P62993 sos_chlae_protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey An amino acid sequence which is not represented in UniProt grb2_human Growth factor receptor-bound protein 2 GRB2 ASH Adapter protein GRB2 SH2/SH3 adapter GRB2 Protein Ash protein protein human Homo sapiens Human MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV 83A4B0BA1B248DC4 pld2-grb2-5 2 7 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P62993:p.Arg86Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R K Wed May 02 18:36:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg86lys' Tue Jun 30 17:35:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg86Lys' ecobb Escherichia coli (strain B / BL21) 4 unspecified role unspecified role prey prey n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association 1 and 2 Purified Grb2 caused an 80% reduction in mycPLD2 bound to Sepharose beads. GSTfusion protein of human Grb2 and a SH2-deficient Grb2 mutant, R86K, were created and tested for the ability to bind mycPLD2 in in vitro GST-pull-down assays. As shown in Figure 2a, purified GSTGrb2 interacts with mycPLD2. pld2-grb2-6 3 4 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined tyr-179 (required) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain undefined mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:00:25 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'phe178-arg180' O14939:p.Tyr179Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 18:18:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr179phe' Tue Jun 30 18:32:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr179Phe' O14939:p.[Tyr179Phe;Asn181Asp] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain N D Wed May 02 15:29:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr179phe,asn181asp' Tue Jun 30 18:26:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr179Phe;Asn181Asp]' 5 unspecified role unspecified role bait bait physical association physical association 3, 4b Mutant plasmids were expressed in COS-7 cells, immunoprecipitated with an anti-Grb2 antibody, and the immunological presence of mycPLD2 was analysed by Westernblot. As shown in Figures 3a and d, a B70% reduction in the coimmunoprecipitated mycPLD2 could be achieved with mycPLD2 Y179F and DY179, suggesting that PLD2 Y179 is involved in Grb2 binding in vivo. pld2-grb2-7 1 4 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O14939:p.Tyr169Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 19:04:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr169phe' Tue Jun 30 18:10:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr169Phe' O14939:p.[Tyr169Phe;Tyr179Phe] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F Fri Jul 22 11:09:24 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr179phe,tyr169phe' Wed May 02 17:02:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr169phe,tyr179phe' Tue Jun 30 19:45:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr169Phe;Tyr179Phe]' O14939:p.Tyr179Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 20:06:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr179phe' Tue Jun 30 15:50:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr179Phe' 5 unspecified role unspecified role prey prey physical association physical association 3 pld2-sos-1 1 4 unspecified role unspecified role bait bait O14939:p.Tyr179Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 15:23:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr179phe' Tue Jun 30 18:26:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr179Phe' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O14939:p.Tyr169Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 14:51:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr169phe' Tue Jun 30 16:19:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr169Phe' 6 unspecified role unspecified role prey prey physical association physical association 7 As shown in Figures 7a and b, mycPLD2 WT interacts with Sos in vivo.