UniProt UniProt http://www.uniprot.org Fission yeast Dss1 associates with the proteasome and is required for efficient ubiquitin-dependent proteolysis. Fission yeast Dss1 associates with the proteasome and is required for efficient ubiquitin-dependent proteolysis. Biochem. J. (0264-6021) 2006 Josse L., Harley ME., Pires IM., Hughes DA. david.a.hughes@manchester.ac.uk 12-Oct-2007: Contacted by JYOTI. davet schpm Schizosaccharomyces pombe (fission yeast) Fission yeast tap tandem affinity purification TAP TAP tap fingerprinting peptide massfingerprinting fingerprinting Josse L., Harley ME., Pires IM., Hughes DA. david.a.hughes@manchester.ac.uk Accepted 2007-OCT-21 by DAVET. Biochem. J. (0264-6021) 2006 davet Fission yeast Dss1 associates with the proteasome and is required for efficient ubiquitin-dependent proteolysis. Fission yeast Dss1 associates with the proteasome and is required for efficient ubiquitin-dependent proteolysis. Biochem. J. (0264-6021) 2006 Josse L., Harley ME., Pires IM., Hughes DA. david.a.hughes@manchester.ac.uk 12-Oct-2007: Contacted by JYOTI. davet schpm Schizosaccharomyces pombe (fission yeast) Fission yeast affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Josse L., Harley ME., Pires IM., Hughes DA. TAP-tagged Dss1 or PA-rpn10 were expressed in S. pombe cells and affinity-purified using IgG-Sepharose in the presence or absence of ATP. david.a.hughes@manchester.ac.uk Accepted 2007-OCT-21 by DAVET. 12-Oct-2007: Contacted by JYOTI. Biochem. J. (0264-6021) 2006 davet rpn3_schpo Probable 26S proteasome regulatory subunit rpn3 rpn3 SPBC119.01 SPBPJ4664.07 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MIDDQRDMQVDSVNQEENVDSGETKQNTVTEVPRTVLQDVEANIAALMQATKQRDPRLVYRSLRTTSNICHRLNADVLGQLIKKYYSFDNSLKNELLELIDMPQNGDDSSTSITNGNGNTIFPEVDMYLQLLLSMTLYYNEKYEVGAEYIKKVIARLQSYDRRTLDQIAAKLYFYYILFFEKCNRSVECRNTLLSVHRTASLRHDSETQAMVLTLLLRNYIQFNLYDQADRLVSKTSFLTNASNNLAIRYQYYLGRIRAIQLDYTTAHEHLVSAIRKAPNTVYAVQFLEAVYKLHIVVQLLMGEIPERRIFRQKSLEKTLVPYLRISQAVRIGDLCAFTDALSKYEAEFRFDGLYTLICRLRHTVIKTGLRMISLSYSRISLRDVCIKLGLDSEESAEYIVAKGIRDGVIDASIDHSNAFMASNEAMDIYSTEQPQQAFHERIQFCLALHNDSIKSMRYPMDAHKSELEGVEEARRRMDKEMAEADLDDDEPDLGEF 85129F750314B0B9 rpn1_schpo 26S proteasome regulatory subunit rpn1 Proteasome non-ATPase subunit mts4 19S regulatory cap region of 26S protease subunit 2 rpn1 mts4 SPBP19A11.03c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSSKDISSKSPSGNDALNDKKGTKTSETNDRNSTNNTKERDELEDLSEEDLQLKNDLELLVQAVQDATPELVGSSLTQLKEIIRTSTSSMTAVPKPLKFLRPHYFTLVKIYDSWPQSPQKTQLADILSVLGMSYSNTSKHESLKYRLQGVTTDPSLWGHEYVRHLASEIEEEFASRQEEEAPTDDLMELALTIVPFFLTHNAEADAIDLLQELGAIEKVVPFVELDNASRVCLYITSCVNLLPFPEDVAMLRTAHAIYRKFDQLTQALNVAIRLDDMSLIKEDCEAATDPLLKKQMSYMLARQQIPMDMGDEELNDALNNTHLSDHFHYLGKELNLMDPKVPEDIFKTHLEVARTGLGASGVYSAKQNLANTFVNALVNAGYSNDRLILVDDEKTSWIYKNKESGLISATASIGLLQLWNVDMGLSLLDKYLYSSEENTKAGALLGIGVTNVAVRNEADPAMAILSEYLETGSVKLRASAILGLGLAYSGANREDLLDMLSPIVTDTDCPMQLSCLAALSLGLIFVGTCNGDVASTILQTLMEREESAQNDQWGRFMALGLALLFNGKQDLADATVETLKAIEGKIARQAEILVDICSYAGTGNVLHIQKLLHICSEPPSDDAKESETTIQTFAALGVATIAMGEDIGAEMVLRHFDHMMHYGEPSIRKAIPLALGLLSASNPQMRIFDTLSRYSHDNDLDVAYNAIFAMGLVGAGTSNARLAQLLRQLASYYHKESNALFMVRIAQGLLYLGKGTMTLNPYHTERQILGQTAFAGLMTVVLAMLDANTFVLDTSHWLLYAITLAIRPRMLITLGEDGQYLPVSVRVGQAVDVVGQAGRPKVITGWVTHTTPVLLHHNERAELATEAYTPLTSLEGIVILKKNTEDIEMTA 03FC9AB767745C5A q94444_chite ORF D protein protein chite Chironomus tentans (Midge) Camptochironomus tentans MKFLAVLALCIVGAVADPISADQADLIRSSWAQVKHNEVDILAAVFKDHPDIQARFPQFAGKDVDSLKDTAAFATHAGRIVGFISEIVALVGNEANRPAMNTLTNELATNHHNRGISKAQFNEFRASMTSYLSHHTTWNDATAAAWTHGLDNIFDAIFAHL 16255522223E90F2 sem1_schpo 26S proteasome complex subunit rpn15 rpn15 dss1 mRNA export factor dss1 sem1 SPAC3G6.02 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSRAALPSLENLEDDDEFEDFATENWPMKDTELDTGDDTLWENNWDDEDIGDDDFSVQLQAELKKKGVAAN 3E86B803266EF456 ublh2_schpo Ubiquitin carboxyl-terminal hydrolase 2 uch2 SPBC409.06 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSWTTIESDAGVFTDLIENLGVKDVEVDELYSLDVDSLRQFPDIYGIIFLFKWNSKVDKPDGTMDYDSMDNIFFAKQVINNACATQALLSVLLNHSDEIDLGTTLSEFKDFSKTLPPELKGEALGNSEHIRCCHNSFARSDPFISEEVRAATDEDEVYHFIAYTNINNVFYELDGLQAAPINHGSCTKEEFAEKAVSVIQARIANYDPAEIRFNLMVICKDKKASLLTREDLTDEEKAASIAVEDEKRLRWKRENQLRRHNFVGLFVELSKLLVKDRIDKNTWNSTLETAKAKYASQKRP 93B13076EF2EDB80 rpn12_schpo 26S proteasome regulatory subunit rpn12 rpn12 mts3 SPBC16G5.01 SPBC342.07 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSTLDLNHLADLYDRKDWNACKKELLKLKVELAKQNLFVPTSDKEKASFARNVFEYGVLVSIQTCDIESFARYASQVIPFYHDSLVPSSRMGLVTGLNLLYLLSENRIAEFHTALESVPDKSLFERDPYVEWVISLEQNVMEGAFDKVASMIRSCNFPEFSYFMKIVMSMVRNEIATCAEKVYSEIPLSNATSLLYLENTKETEKLAEERGWDIRDGVIYFPKEANALETEDGMLIDEEDELELPPTASKHTISSIRQLLSYTSELEQIV C1BC6571722A034D rpn8_schpo 26S proteasome regulatory subunit rpn8 rpn8 SPCC1682.10 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MPPAVSSETSTIVPQQVIVHPLVLLSAVDSYNRSAKGTKRRVVGILLGQNNGDVVNVANSYAIPFEEDEKNASVWFLDHNFMESMNEMFKKINANEKLVGWYHTGPQLRPSDLEINNLLKKYIPNPVLVIIDVKPKSVGLPTNAYFAIDEIEDDGSKSSRTFVHLPSSIEAEEAEEIGVEHLLRDTRDASVGTLATRVTQQAQSLQGLGQRLTEIADYLRKVVDGQLPINHAILAELQSVFNLLPNIFSGPVVSEQALESEAQRAFNVNSNDQLMSIYISSIVRAVIALHDLLDSLAASKAMEQQDIKPTVQNGEVSANAEQKA 538538D1863789E6 rpn10_schpo 26S proteasome regulatory subunit rpn10 rpn10 pus1 SPAC637.10c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MVLEATMILIDNSEWMINGDYIPTRFEAQKDTVHMIFNQKINDNPENMCGLMTIGDNSPQVLSTLTRDYGKFLSAMHDLPVRGNAKFGDGIQIAQLALKHRENKIQRQRIVAFVGSPIVEDEKNLIRLAKRMKKNNVAIDIIHIGELQNESALQHFIDAANSSDSCHLVSIPPSPQLLSDLVNQSPIGQGVVASQNQFEYGVDPNLDVELALALELSMAEERARQEVAAQKSSEETEDKKMQE 9ED58AE82687F79E rpn2_schpo 26S proteasome regulatory subunit rpn2 rpn2 SPBC17D11.07c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MTMVYSDNTSIITSAGGLMALLDEQERELQVHALLKIYEFIDQLWPEISDDVTKIEVMYEDHSFPERELAALVVSKVYYYLGEYDEALLFALSSGPKFLHDKNSDYKETLIFKCIDMFIHKSAELYKNPKADPLDERLSGVVEGIFQKCYAKNEWRHVLGIAIEAHRLDIIEYILNADKETDLKPYVLELAMTVVLDIEFRNRLLRLLLSSFLTETEPDYFSVGKCVVHLNDASVAAKLLMKLSSQNDDKSLLTAYQLAFDLEDSAPQEFLNSVMDLLPSPSVANSEEDANADSKKEDSSPCGYIIRILSGEQTVKYDREFLYAHNNTDMLILNRTKDSLEARNSVFHNAVTFANAFMNFGTSSDSFFRDNLSWLSKANNWSKFTATAALGVIHRGYYNQAMNILRPYLPEEDAPSSSTYSEGGAFYAMGLIHANHGRGVTEYLREQLKHTEDEIVQYGLLLGIGLTGMASRDETLYESVKTILFNDNAVAGSAAGISMGLIMLGTASSAAIDEMLQYAHETQHEKIIRGLGIGIALIVYGRQQEADGIIKELNNDLDPTLRYAGMFATALAYCGTSNSKIVRDVLHISVSDVNDDVRRAAVCALGFICFKDPNALISTVELLVDSYNPHVRYGSAIALGIACANSGSNAALDLLSRLVEDATDFVRQGAMIAQAMILTQHNDQLNSKVSGIRKHFEQVINEKHEDALAKLGATLAQGIIDAGGRNVTIALQTATGSLKLSAIVGLTVFLQYWYWFPLTHFMSLSFSPTALIGLDKNLNAPKFSFISNVRPKLFAYPPKSVQPTAKTVQKVETAVLSTTVKAQARAKRAEREKASKGSNDDEMKIDKKTTEEKEATPMEMDEEKSQDISINGNSKKEEPKSETLENFTRVVPAQLPYISFNLNGRYYPVRKFTGGVLMLIDRESDKAPDLIELNRDAVPASADTEPGEQEASPPEDFEYPFDDDD E8D1758722127F31 dss1-rpn2-rpn3-1 1 11 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 2A Additional identified proteins co-purifying with TAP-dss1 (3 independent experiments): P33612 (Mts2/Rpt2), O74894 (Rpt3/SPCC576.10), O14126 (Rpt5/Tbp1/Pam2), Q9UTM3(Rpn5), Q10335 (Rpn7/SOBC582.07c), O42839 (Cut20/Lid1/Apc4) and Q10311 (Nas6/SPAC6C3.08). dss1-rpn1-1 2 6 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey physical association physical association The interaction was independent on ATP. An association of dss1 with the 20S proteasome core particle was dependent on ATP. 2B rpn10-rnp1-1 2 4 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association The interaction was independent on ATP. An association of rnp10 with the 20S proteasome core particle was enhanced by ATP. 2B