UniProt UniProt http://www.uniprot.org P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. Mol. Biol. Cell (1059-1524) 2005 Fox AH., Bond CS., Lamond AI. a.i.lamond@dundee.ac.uk 13-Nov-2007: Contacted by JYOTI. davet human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Fox AH., Bond CS., Lamond AI. Cells have been treated with actinomycin D. a.i.lamond@dundee.ac.uk Accepted 2007-NOV-3 by DAVET. 13-Nov-2007: Contacted by JYOTI. Mol. Biol. Cell (1059-1524) 2005 davet P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. Mol. Biol. Cell (1059-1524) 2005 Fox AH., Bond CS., Lamond AI. a.i.lamond@dundee.ac.uk 13-Nov-2007: Contacted by JYOTI. davet human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip antibody detection identification by antibody antibody detection Fox AH., Bond CS., Lamond AI. a.i.lamond@dundee.ac.uk Accepted 2007-NOV-3 by DAVET. 13-Nov-2007: Contacted by JYOTI. Mol. Biol. Cell (1059-1524) 2005 davet P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. Mol. Biol. Cell (1059-1524) 2005 Fox AH., Bond CS., Lamond AI. a.i.lamond@dundee.ac.uk 13-Nov-2007: Contacted by JYOTI. davet human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Fox AH., Bond CS., Lamond AI. a.i.lamond@dundee.ac.uk Accepted 2007-NOV-3 by DAVET. 13-Nov-2007: Contacted by JYOTI. Mol. Biol. Cell (1059-1524) 2005 davet P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. Mol. Biol. Cell (1059-1524) 2005 Fox AH., Bond CS., Lamond AI. a.i.lamond@dundee.ac.uk 13-Nov-2007: Contacted by JYOTI. davet human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip fingerprinting peptide massfingerprinting fingerprinting Fox AH., Bond CS., Lamond AI. a.i.lamond@dundee.ac.uk Accepted 2007-NOV-3 by DAVET. Mol. Biol. Cell (1059-1524) 2005 davet P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. Mol. Biol. Cell (1059-1524) 2005 Fox AH., Bond CS., Lamond AI. a.i.lamond@dundee.ac.uk 13-Nov-2007: Contacted by JYOTI. davet in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Fox AH., Bond CS., Lamond AI. His-tagged PSPC1 and untagged NONO fragments have been coexpressed in E.coli.. Lysates have been loaded to Ni-agarose and subsequently gel filtration to purify His-tagged PSPC1 was applied. a.i.lamond@dundee.ac.uk Accepted 2007-NOV-3 by DAVET. 13-Nov-2007: Contacted by JYOTI. Mol. Biol. Cell (1059-1524) 2005 davet pspc1_human Paraspeckle component 1 PSPC1 PSP1 Paraspeckle protein 1 protein protein human Homo sapiens Human MMLRGNLKQVRIEKNPARLRALESAVGESEPAAAAAMALALAGEPAPPAPAPPEDHPDEEMGFTIDIKSFLKPGEKTYTQRCRLFVGNLPTDITEEDFKRLFERYGEPSEVFINRDRGFGFIRLESRTLAEIAKAELDGTILKSRPLRIRFATHGAALTVKNLSPVVSNELLEQAFSQFGPVEKAVVVVDDRGRATGKGFVEFAAKPPARKALERCGDGAFLLTTTPRPVIVEPMEQFDDEDGLPEKLMQKTQQYHKEREQPPRFAQPGTFEFEYASRWKALDEMEKQQREQVDRNIREAKEKLEAEMEAARHEHQLMLMRQDLMRRQEELRRLEELRNQELQKRKQIQLRHEEEHRRREEEMIRHREQEELRRQQEGFKPNYMENREQEMRMGDMGPRGAINMGDAFSPAPAGNQGPPPMMGMNMNNRATIPGPPMGPGPAMGPEGAANMGTPMMPDNGAVHNDRFPQGPPSQMGSPMGSRTGSETPQAPMSGVGPVSGGPGGFGRGSQGGNFEGPNKRRRY 3A8C44079D64BBF0 nono_human Non-POU domain-containing octamer-binding protein NONO NRB54 54 kDa nuclear RNA- and DNA-binding protein p54(nrb) 55 kDa nuclear protein NMT55 DNA-binding p52/p100 complex, 52 kDa subunit protein protein human Homo sapiens Human MQSNKTFNLEKQNHTPRKHHQHHHQQQHHQQQQQQPPPPPIPANGQQASSQNEGLTIDLKNFRKPGEKTFTQRSRLFVGNLPPDITEEEMRKLFEKYGKAGEVFIHKDKGFGFIRLETRTLAEIAKVELDNMPLRGKQLRVRFACHSASLTVRNLPQYVSNELLEEAFSVFGQVERAVVIVDDRGRPSGKGIVEFSGKPAARKALDRCSEGSFLLTTFPRPVTVEPMDQLDDEEGLPEKLVIKNQQFHKEREQPPRFAQPGSFEYEYAMRWKALIEMEKQQQDQVDRNIKEAREKLEMEMEAARHEHQVMLMRQDLMRRQEELRRMEELHNQEVQKRKQLELRQEEERRRREEEMRRQQEEMMRRQQEGFKGTFPDAREQEIRMGQMAMGGAMGINNRGAMPPAPVPAGTPAPPGPATMMPDGTLGLTPPTTERFGQAATMEGIGAIGGTPPAFNRAAPGAEFAPNKRRRY DIP protein Q15233 original sequence version: 156 DIP protein Q15233 original sequence version: 148 DIP protein Q15233 original sequence version: 139 DIP protein Q15233 original sequence version: 91 26BBD3828F5B9E49 sfpq_human Splicing factor, proline- and glutamine-rich SFPQ PSF Polypyrimidine tract-binding protein-associated-splicing factor DNA-binding p52/p100 complex, 100 kDa subunit 100 kDa DNA-pairing protein protein protein human Homo sapiens Human MSRDRFRSRGGGGGGFHRRGGGGGRGGLHDFRSPPPGMGLNQNRGPMGPGPGQSGPKPPIPPPPPHQQQQQPPPQQPPPQQPPPHQPPPHPQPHQQQQPPPPPQDSSKPVVAQGPGPAPGVGSAPPASSSAPPATPPTSGAPPGSGPGPTPTPPPAVTSAPPGAPPPTPPSSGVPTTPPQAGGPPPPPAAVPGPGPGPKQGPGPGGPKGGKMPGGPKPGGGPGLSTPGGHPKPPHRGGGEPRGGRQHHPPYHQQHHQGPPPGGPGGRSEEKISDSEGFKANLSLLRRPGEKTYTQRCRLFVGNLPADITEDEFKRLFAKYGEPGEVFINKGKGFGFIKLESRALAEIAKAELDDTPMRGRQLRVRFATHAAALSVRNLSPYVSNELLEEAFSQFGPIERAVVIVDDRGRSTGKGIVEFASKPAARKAFERCSEGVFLLTTTPRPVIVEPLEQLDDEDGLPEKLAQKNPMYQKERETPPRFAQHGTFEYEYSQRWKSLDEMEKQQREQVEKNMKDAKDKLESEMEDAYHEHQANLLRQDLMRRQEELRRMEELHNQEMQKRKEMQLRQEEERRRREEEMMIRQREMEEQMRRQREESYSRMGYMDPRERDMRMGGGGAMNMGDPYGSGGQKFPPLGGGGGIGYEANPGVPPATMSGSMMGSDMRTERFGQGGAGPVGGQGPRGMGPGTPAGYGRGREEYEGPNKKPRF DIP protein P23246 original sequence version: 182 6D8D5EA95E235847 pspc1-nono-1 4 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 5B Increasing the stringency of washing the beads did not change the interaction therefore appears to be stable. nono-pspc1-1 2 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association 5D nono-nono-1 2 7 unspecified role unspecified role bait bait region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 7 unspecified role unspecified role prey prey physical association physical association 5D pspc1-nono-2 2 6 unspecified role unspecified role bait bait region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 7 unspecified role unspecified role prey prey physical association physical association 6 pspc1-nono-3 3 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 8A Inhibition of PolII transcription did not change the interaction. pspc1-nono-4 5 6 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 8B pspc1-sfpq-1 1 6 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 colocalization colocalization 7D