IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The IRREGULAR TRICHOME BRANCH loci regulate trichome elongation in Arabidopsis. The IRREGULAR TRICHOME BRANCH loci regulate trichome elongation in Arabidopsis. Plant Cell Physiol. (0032-0781) 2005 Zhang X., Grey PH., Krishnakumar S., Oppenheimer DG. doppen@botany.ufl.edu 22-Jan-2008: Contacted by JYOTI. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Zhang X., Grey PH., Krishnakumar S., Oppenheimer DG. doppen@botany.ufl.edu Accepted 2007-DEC-14 by JYOTI. Plant Cell Physiol. (0032-0781) 2005 brk1_arath Protein BRICK 1 BRK1 T9I22.8 At2g22640 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAKAGGITNAVNVGIAVQADWENREFISHISLNVRRLFEFLVQFESTTKSKLASLNEKLDLLERRLEMLEVQVSTATANPSLFAT CEF8D18BEB4B761C scar2_arath Protein SCAR2 WAVE4 Protein WAVE4 Protein DISTORTED 3 Protein IRREGULAR TRICHOME BRANCH 1 SCAR2 DIS3 ITB1 T19C21.7 At2g38440 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MPLTRYQSRNEYGLADPDLYQAADKDDPEALLEGVAMAGLVGILRQLGDLAEFAAEMFHDLHEEVMATASRSHGLMARVQQLEAEFPSIEKALLCQTDHSPFFSNKGVEWHPNLQLEQSVVTSGDLPRCVMDSYEECRGPPRLFLLDKFDISGAGACLKRYTDPSFVRLETSSYEESWDDIQREKKSQKAKRRASQWRNGGTPENALSSHAKLHELFLEEHLEAHHSDPARVVKLKTRKLDGCSLISKSGESYMEKFVQTRVDSKISYEIITQNPGLLTWNMDSARDVVTDIPEISMVGAMDKSHGGSRAEVSFPSEQENVANVNMNGGFIEKDIETVPESTYNEVRGTTITQDSQTVLNGKPGFFQQRSYSEDLTSEADNYVDAPATMESETETDDECRPKSRSDTLKDGNHHIYSDAVEERMEDPPQFSFSHSNGNTPVSENGRSSFGKKSTSYSYSDTASISIDDQSDGEKLSGCLPSTSSFKSELVDSMSHVTPEANKVSHDLNVQESVSSSNVDGQTSLSSNGTCSSPRPVSQNDQSCSLTVQSLASEVVETSPELVRLDLMKGGNDGRKVDPFDSSKSCASFDAKNSDLPSETSSISSTSEGSRCDSTIEKNCMVASNLVNSGTSPQAFVDSQTGKQLPIADTDFETNSIVACSEVLANSGSDPEERDGRCLTGKLVPCSAGVGMEVSPDTPSKVCGPSSADGIHLKDTLDDETDCVSVTNVVVDVDSKNSVADVGSQSSVADIDSQSSVAEISDEHSCAFGNTADVSVSESHEDTLENGMSVPSDFNSGVEKLAGDASPTCSKCDDHISHEGFHDLSGLDNATTDIVPNVELDVSDNDNDTSSGGVNHAVSLSSTRGKGSLPWISTNTYQSSSDAGEIFHDTVVESDGTLLEDNNPESEIKMHKSPLEVSSEGLSTEPDNKDVESIESTSPKPSLDQRNRDTETKSPGESILDDNCIDSTQVYNLNLLESEAIDQAVREQTSYASHEVADEELLQSNVFRGLEFEPQSAGLEFAPQSAGIELNRPKQELNLDPTFPSFGFIPETIPPNPEDMPPLPPMQWLIGKVPHSFPTFMGESVETSSSALSAAPPIGSSLNVQIGSPPSELSVSLGSDESERLPGGFVHNASEKPLQSSIQFPTMSTDLNSQYDSSELPTIPYQECIEDFGSEENNLLADHAAQNHELVYSQASSLQLPQVKHEDFKDDADVHESQSSSDDHHCPETKSLTPTQSTKVEDKGHSVPDASNAETAESSNTSVQKINPVSVGDAMWPVSCFSVAPTLDTYKTEVVPTVRLPRPRSPLVDAVAAHDRRKMKKVSEMVHPPIKSKQDDKDSLLAQIRNKSVNLKPAVTTRPSIQTGPRTDLRVAAILEKANTIRMAMAGSDEDEDSDSWSDS CAC55D7CF3C8AEBC brick1-scar2 1 3 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 2 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 7 As expected, we found that BRK1 bound to the SHD of ITB1/SCAR2 (Figure 7, lanes 7 and 8; note presence of ITB SHD band in lane 8). However, BRK1 did not bind to an 1106�amino acid fragment that did not contain the SHD (Figure 7, lanes 5 and 6; note absence of ITBDSHD band in lane 4). This result shows that the SHD of ITB1/SCAR2 is both necessary and sufficient for binding to BRK1.