IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Repression of light signaling by Arabidopsis SPA1 involves post-translational regulation of HFR1 protein accumulation. Repression of light signaling by Arabidopsis SPA1 involves post-translational regulation of HFR1 protein accumulation. Plant J. (0960-7412) 2005 imex curation Yang J., Lin R., Hoecker U., Liu B., Xu L., Wang H. hw75@cornell.edu 22-Jan-2008: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Yang J., Lin R., Hoecker U., Liu B., Xu L., Wang H. hw75@cornell.edu Accepted 2007-DEC-12 by ORCHARD. Plant J. (0960-7412) 2005 orchard Only protein-protein interactions imex curation Repression of light signaling by Arabidopsis SPA1 involves post-translational regulation of HFR1 protein accumulation. Repression of light signaling by Arabidopsis SPA1 involves post-translational regulation of HFR1 protein accumulation. Plant J. (0960-7412) 2005 imex curation Yang J., Lin R., Hoecker U., Liu B., Xu L., Wang H. hw75@cornell.edu 22-Jan-2008: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Yang J., Lin R., Hoecker U., Liu B., Xu L., Wang H. hw75@cornell.edu Accepted 2007-DEC-12 by ORCHARD. 22-Jan-2008: Contacted by JYOTI. Plant J. (0960-7412) 2005 orchard Only protein-protein interactions imex curation spa1_arath Protein SUPPRESSOR OF PHYA-105 1 SPA1 F11C10.3/F11C10.4 At2g46340/At2g46350 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MPVMERVAEETVATNNIQLKARVDDVPCNKLDARHNDMVIQSETANSDCPGSSAHRNVDLTKPPPPEEAAGAKLSVEELTLGNYRIVQGSNNTNVDSPRAGKFEHLYRLARGSAFRAGDGDLDSQPRDMDQMLSRIRQQLAGAPSERQNLKPFMSRRSDQNLEAFSERLRAAGENSIMNAPALISEGVQMKTPVSSSNFSQLLLKRAMKGKGVVGKNQETPPEFVSDQDLGSKEKKLDISKSPTPHDVLPLKSSPKGNGMVSHGDGNHSKSSIGISLREFLRSSYAKREKRHGLCLFRQLVELVDSAHSKRLFLLDLRPSLFTLVPSKKLRYIGNFGKNDLESDVDEDLNRRRPVVEESSSGGRDSKKRKMDLHLNSPGNQLQATSTGRPFKRKSPVIDLNMVDARNPDSCELQQQDYIKNLSVSSVSRKQSMSTWLEEQWYTCPEEINGEDIGEKSNIYALGVLLFELLCHCESGEMHAAMMADLRHRILPPTFLSKYPKEAGFCLWLLHPEPSSRPSARDILKSELICEDDSVKSTAAAEEISELLLHFLSSLEVQKKKKASKLLQDIQTLEDDIKEAERRYSSNVSLVRSHGAIEKRVQSSPLDEHCTTSSALFVPTANTDRLMSNIRQLEDAYFFMRSQINLSSSAATARSDKTLKDRDRCSENQNENQDMSTKGKSSDQLEVFFEGLCKFARYSKFETCGTIRSGDLLNSASVVCSLSFDPDEEHIAAAGISKKIKIFDFNAFMNESVGVHYPLVEMVNKSKLSCVCWNSYIKNYLASTDYDGVVQIWDAGTGQGFSQYTEHQKRAWSVDFSPSDPTKFVSGSDDCSVKLWSINEKRSLGTIWSPANVCCVQFSSYSNHLLAFGSADYKVYCYDLRYVKTPWCTLAGHEKAVSYVKFMDSETIVSASTDNSLKLWNLNKTNSSGLSPGACSLTYKGHTNQKNFVGLSVLDGYIACGSETNEVYSYYKSLPMPMTSYKFGSVDPISGNEYFDDNGQFVSSVCWRKKSNMLVAANSTGNMKLLKLV DIP protein Q9SYX2 original sequence version: 102 8DEDF32BB06B4A18 hfr1_arath Transcription factor HFR1 Transcription factor EN 68 bHLH transcription factor bHLH026 HFR1 BHLH26 EN68 Basic helix-loop-helix protein 26 Reduced sensitivity to far-red light Protein LONG HYPOCOTYL IN FAR-RED 1 FBI1 Protein REDUCED PHYTOCHROME SIGNALING REP1 RSF1 At1g02340 T6A9.4 T6A9_13 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSNNQAFMELGWRNDVGSLAVKDQGMMSERARSDEDRLINGLKWGYGYFDHDQTDNYLQIVPEIHKEVENAKEDLLVVVPDEHSETDDHHHIKDFSERSDHRFYLRNKHENPKKRRIQVLSSDDESEEFTREVPSVTRKGSKRRRRDEKMSNKMRKLQQLVPNCHKTDKVSVLDKTIEYMKNLQLQLQMMSTVGVNPYFLPATLGFGMHNHMLTAMASAHGLNPANHMMPSPLIPALNWPLPPFTNISFPHSSSQSLFLTTSSPASSPQSLHGLVPYFPSFLDFSSHAMRRL BC75C5EB26E10A20 spa1-hfr1 1 4 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 4 Thus, the coiled-coil region of SPA1 appears to be required, and sufficient, for mediating the interaction between SPA1 and HFR1, hfr1-spa1 1 4 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association 4 Thus, the coiled-coil region of SPA1 appears to be required, and sufficient, for mediating the interaction between SPA1 and HFR1, The authors found that both full-length HFR1 as well as a number of deletion mutant forms of HFR1,were all capable of interacting with full length SPA1. They concluded that these "observations suggest that the overall three-dimensional structure of HFR1 might be important for HFR1-SPA1 interaction." In this case individual regions of HFR1 which interacted with SPA1 were ommitted because they could confuse the user. It is clear from the result of this experiment that it is the tertiary structure of HFR1 which is important in the interaction not the primary structure. hfr1-spa1-1 2 3 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 4 unspecified role unspecified role bait bait region GAD tagged tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction 5 The coiled-coil domain itself (SPA1-CC) also interacted with HFR1 The authors indicate that the GAD-HFR1 was partially labeled. It was unclear how to represent the fact that the GAD-HFR1 was partially labeled.