IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ DISTORTED3/SCAR2 is a putative arabidopsis WAVE complex subunit that activates the Arp2/3 complex and is required for epidermal morphogenesis. DISTORTED3/SCAR2 is a putative arabidopsis WAVE complex subunit that activates the Arp2/3 complex and is required for epidermal morphogenesis. Plant Cell (1040-4651) 2005 Basu D., Le J., El-Essal Sel-D., Huang S., Zhang C., Mallery EL., Koliantz G., Staiger CJ., Szymanski DB. Actin polyerisation studies are not entered as the actin protein being used in the study is not identified (e.g. skeletal or smoot muscle actin). dszyman@purdue.edu 01-Feb-2008: Contacted by JYOTI. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Basu D., Le J., El-Essal Sel-D., Huang S., Zhang C., Mallery EL., Koliantz G., Staiger CJ., Szymanski DB. Actin polyerisation studies are not entered as the actin protein being used in the study is not identified (e.g. skeletal or smoot muscle actin). dszyman@purdue.edu Accepted 2008-FEB-9 by CDEROW. 01-Feb-2008: Contacted by JYOTI. Plant Cell (1040-4651) 2005 DISTORTED3/SCAR2 is a putative arabidopsis WAVE complex subunit that activates the Arp2/3 complex and is required for epidermal morphogenesis. DISTORTED3/SCAR2 is a putative arabidopsis WAVE complex subunit that activates the Arp2/3 complex and is required for epidermal morphogenesis. Plant Cell (1040-4651) 2005 Basu D., Le J., El-Essal Sel-D., Huang S., Zhang C., Mallery EL., Koliantz G., Staiger CJ., Szymanski DB. Actin polyerisation studies are not entered as the actin protein being used in the study is not identified (e.g. skeletal or smoot muscle actin). dszyman@purdue.edu 01-Feb-2008: Contacted by JYOTI. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Basu D., Le J., El-Essal Sel-D., Huang S., Zhang C., Mallery EL., Koliantz G., Staiger CJ., Szymanski DB. Actin polyerisation studies are not entered as the actin protein being used in the study is not identified (e.g. skeletal or smoot muscle actin). dszyman@purdue.edu Accepted 2008-FEB-9 by CDEROW. 01-Feb-2008: Contacted by JYOTI. Plant Cell (1040-4651) 2005 wasf1_human Wiskott-Aldrich syndrome protein family member 1 WASF1 KIAA0269 SCAR1 WAVE1 Protein WAVE-1 Verprolin homology domain-containing protein 1 protein protein human Homo sapiens Human MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSLSKYAEDIFGELFNEAHSFSFRVNSLQERVDRLSVSVTQLDPKEEELSLQDITMRKAFRSSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNILTPYRDDGKEGLKFYTNPSYFFDLWKEKMLQDTEDKRKEKRKQKQKNLDRPHEPEKVPRAPHDRRREWQKLAQGPELAEDDANLLHKHIEVANGPASHFETRPQTYVDHMDGSYSLSALPFSQMSELLTRAEERVLVRPHEPPPPPPMHGAGDAKPIPTCISSATGLIENRPQSPATGRTPVFVSPTPPPPPPPLPSALSTSSLRASMTSTPPPPVPPPPPPPATALQAPAVPPPPAPLQIAPGVLHPAPPPIAPPLVQPSPPVARAAPVCETVPVHPLPQGEVQGLPPPPPPPPLPPPGIRPSSPVTVTALAHPPSGLHPTPSTAPGPHVPLMPPSPPSQVIPASEPKRHPSTLPVISDARSVLLEAIRKGIQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE DIP protein Q92558 original sequence version: 78 DIP protein Q92558 original sequence version: 82 DIP protein Q92558 original sequence version: 124 DIP protein Q92558 original sequence version: 87 44B4527BDB77BC6E abil1_arath Protein ABIL1 Abl interactor-like protein 1 ABIL1 T3F17 At2g46225 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress METEISGMDNPAMTLDEVSMERNKSFVKALQELKNLRPQLYSAADYCEKSYLHSEQKQMVLDNLKDYTVKALVNAVDHLGTVASKLTDLFDHQNSDISTMEMRASCVSQQLLTCRTYIDKEGLRQQQLLAVIPLHHKHYILPNSVNKRVHFSPLRRTDTRQNHYQAISRLQPSDAPASKSLSWHLGSETKSTLKGTSTVAPSSKDSKAFSKTSGVFHLLGDDENIANKKPLAGSQVSGVPAASTAHKDLEVPKLLTAHRSLDNNPRREIIQAPVRTKSVLSAFFVKQKTPKLKAGYVS 11C7CBC733886794 nckp1_human Nck-associated protein 1 NCKAP1 HEM2 KIAA0587 NAP1 p125Nap1 Membrane-associated protein HEM-2 protein protein human Homo sapiens Human MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA DIP protein Q9Y2A7 original sequence version: 81 DIP protein Q9Y2A7-1 original sequence version: 85 DIP protein Q9Y2A7 original sequence version: 85 DIP protein Q9Y2A7 original sequence version: 129 12D32BAC7080CB5A arpc3_arath Actin-related protein 2/3 complex subunit 3 ARPC3 Actin-related protein C3 Arp2/3 complex 21 kDa subunit T13D8.30 At1g60430 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVYHSSFVDEEGVTKACGCPLLPLKSHIKGPAPVSEQDKTDIVDEAITFFRANVFFTNFDIKSPADKLLIYLTFYINVALKRLEGCRTLAVGTKAIINLGLEDIPVPGETGFPFPGLFSLPQSQDEAELFRNYLKQVREETSGRLLSVAYRANGTPNKWWLAFAKRKFMNVVVL 9383A162D10E62CA scar2_arath Protein SCAR2 WAVE4 Protein WAVE4 Protein DISTORTED 3 Protein IRREGULAR TRICHOME BRANCH 1 SCAR2 DIS3 ITB1 T19C21.7 At2g38440 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MPLTRYQSRNEYGLADPDLYQAADKDDPEALLEGVAMAGLVGILRQLGDLAEFAAEMFHDLHEEVMATASRSHGLMARVQQLEAEFPSIEKALLCQTDHSPFFSNKGVEWHPNLQLEQSVVTSGDLPRCVMDSYEECRGPPRLFLLDKFDISGAGACLKRYTDPSFVRLETSSYEESWDDIQREKKSQKAKRRASQWRNGGTPENALSSHAKLHELFLEEHLEAHHSDPARVVKLKTRKLDGCSLISKSGESYMEKFVQTRVDSKISYEIITQNPGLLTWNMDSARDVVTDIPEISMVGAMDKSHGGSRAEVSFPSEQENVANVNMNGGFIEKDIETVPESTYNEVRGTTITQDSQTVLNGKPGFFQQRSYSEDLTSEADNYVDAPATMESETETDDECRPKSRSDTLKDGNHHIYSDAVEERMEDPPQFSFSHSNGNTPVSENGRSSFGKKSTSYSYSDTASISIDDQSDGEKLSGCLPSTSSFKSELVDSMSHVTPEANKVSHDLNVQESVSSSNVDGQTSLSSNGTCSSPRPVSQNDQSCSLTVQSLASEVVETSPELVRLDLMKGGNDGRKVDPFDSSKSCASFDAKNSDLPSETSSISSTSEGSRCDSTIEKNCMVASNLVNSGTSPQAFVDSQTGKQLPIADTDFETNSIVACSEVLANSGSDPEERDGRCLTGKLVPCSAGVGMEVSPDTPSKVCGPSSADGIHLKDTLDDETDCVSVTNVVVDVDSKNSVADVGSQSSVADIDSQSSVAEISDEHSCAFGNTADVSVSESHEDTLENGMSVPSDFNSGVEKLAGDASPTCSKCDDHISHEGFHDLSGLDNATTDIVPNVELDVSDNDNDTSSGGVNHAVSLSSTRGKGSLPWISTNTYQSSSDAGEIFHDTVVESDGTLLEDNNPESEIKMHKSPLEVSSEGLSTEPDNKDVESIESTSPKPSLDQRNRDTETKSPGESILDDNCIDSTQVYNLNLLESEAIDQAVREQTSYASHEVADEELLQSNVFRGLEFEPQSAGLEFAPQSAGIELNRPKQELNLDPTFPSFGFIPETIPPNPEDMPPLPPMQWLIGKVPHSFPTFMGESVETSSSALSAAPPIGSSLNVQIGSPPSELSVSLGSDESERLPGGFVHNASEKPLQSSIQFPTMSTDLNSQYDSSELPTIPYQECIEDFGSEENNLLADHAAQNHELVYSQASSLQLPQVKHEDFKDDADVHESQSSSDDHHCPETKSLTPTQSTKVEDKGHSVPDASNAETAESSNTSVQKINPVSVGDAMWPVSCFSVAPTLDTYKTEVVPTVRLPRPRSPLVDAVAAHDRRKMKKVSEMVHPPIKSKQDDKDSLLAQIRNKSVNLKPAVTTRPSIQTGPRTDLRVAAILEKANTIRMAMAGSDEDEDSDSWSDS CAC55D7CF3C8AEBC nckp1_arath Protein NAP1 Nck-associated protein 1 p125Nap1 NAP of plants ARP2/3 regulatory protein subunit NAPP Protein GNARLED NAP1 GRL NAP NAPP T4C15.22 At2g35110 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MANSRQYYPSQDESMSPTSVRSREWEGPSRWTEYLGPEMAASVSSTRSSKQIDGHVGGSTKALNIQWVVQMIEVADGLMAKMYRLNQILEYPDPVGHVFSEAFWKAGVFPNHPRICTLLSKKFPEHFSKLQLERIDKFSLDSLHDGAELHLQSLEPWIQLLLDLMAFREQALRLILDLSSTVITLLPHQNSLILHAFMDLFCAFVRVNLFAEKIPRKMLLQVYNLLHALSRNDRDCDFYHRLVQFIDSYDPPLKGLQEDLNFVSPRIGEVLEAVGPSIFLSADTRKLRNEGFLSPYHPRFPDILTNSAHPMRAQDLANVTSYREWVLLGYLVCPDELLRVTSIDIALVVLKENLVVTLFRDEVSLYQMVCEKEFGIGISFASADSINLTMQYILLHEDYQLYVLPRVLESKKMAKSGRTKQKEADLEYSVAKQVEKMISEVHEQALQLCDTIHRERRILLKQEIGRMVLFFTDQPSLLAPNIQMVFSALALAQSEVLWYFQHAGIASSRSKAARVIPVDIDPNDPTIGFLLDGMDRLCCLVRKYISAARGYALSYLSSSAGRIRYLMGTPGIVALDLDPTLKGLFQRIVQHLESIPKAQGENVSAITCDLSDFRKDWLSILMIVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESQLSKHGSLKKLYFYHQHLTTVFRNTMFGPEGRPQHCCAWLSVASSFPECASLIIPEEVTKFGRDAVLYVESLIESIMGGLEGLINILDSEGGFGALESQLLPEQAAAYLNNASRISAPSVKSPRVVGGFTLPGHESYPENNKSIKMLEAAIQRLTNLCSILNDMEPICVINHVFVLREYMRECILGNFKRRFLTALQTDNDLQRPSVLESLIRRHMGIVHLAEQHVSMDLTQGIREILLTEAFSGPVSSLHTFEKPAEQQQTTGSAVEVVCNWYMDNIIKDVSGAGILFAPRHKYFKSTRPVGGYFAESVTDLKELQAFVRIFGGYGVDRLDRMMKVHTAALVNCIETSLRSNRELIEAAAASMHSGDRVERDASVRQIVDLDTVIGFCIEAGQALAFDDLLAEASGAVLEDNASLIHSMISGIVEHIPEEIPEKKEIRRIKGVANGVGVAGDHDSEWVRLILEEVGGANDNSWSLLPYFFASFMTSNAWNTTGFNIETGGFSNNIHCLARCISAVIAGSEYVRLQREYQQQHQSLSNGHHSSENLDSEFPPRVTAEASIKSSMLLFVKFAASIVLDSWSEANRSHLVAKLIFLDQLCEISPYLPRSSLESHVPYTILRSIYTQYYSNTPSTPLSTASPYHSPSVSLIHASPSMKNSTTPQRGSGSGSSSTAAPDSGYFKGSSSSLYGQEHYTESETGNSRNNENNNNNKQRGSSRRSGPLDYSSSHKGGSGSNSTGPSPLPRFAVSRSGPISYKQHN DIP protein Q5S2C4 original sequence version: 24 A2008CEC598BB6F1 abil1-nap1-1 1 4 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 10 A ABIL1 interacted with both human NAP1 and GRL (NAP1) in the yeast two-hybrid assay. abil1-nckap1 1 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 10 A ABIL1 interacted with both human NAP1 and GRL (NAP1) in the yeast two-hybrid assay. abil1-scar2 1 7 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role bait bait physical association physical association 10 B The SHD domain of DIS3/SCAR2 interacts with ABIL1 in the yeast two-hybrid assay. arpc3-scar2 1 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey c-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association 11 A DIS3/SCAR2 and human WAVE1 interact with ARPC3 in a yeast twohybrid assay. arpc3-wasf1 1 6 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association 11 DIS3/SCAR2 and human WAVE1 interact with ARPC3 in a yeast twohybrid assay. scar2-arpc3-1 2 6 unspecified role unspecified role prey prey n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 7 unspecified role unspecified role bait bait c-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 11 C Direct interaction of DIS3/SCAR2 and ARPC3 in a GST pull-down assay.