IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Activation of Arp2/3 complex-dependent actin polymerization by plant proteins distantly related to Scar/WAVE. Activation of Arp2/3 complex-dependent actin polymerization by plant proteins distantly related to Scar/WAVE. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2004 Frank M., Egile C., Dyachok J., Djakovic S., Nolasco M., Li R., Smith LG. It is not possible to enter datafrom the actin polymerisation studies since the composition of the Bovine Arp2/3 complex is unknown. Lsmith@biomail.ucsd.edu 22-Jan-2008: Contacted by JYOTI. orchard in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Frank M., Egile C., Dyachok J., Djakovic S., Nolasco M., Li R., Smith LG. It is not possible to enter datafrom the actin polymerisation studies since the composition of the Bovine Arp2/3 complex is unknown. Lsmith@biomail.ucsd.edu Accepted 2007-DEC-6 by ORCHARD. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2004 orchard scar3_arath Protein SCAR3 Protein WAVE2 SCAR3 F28N24.14 At1g29170 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MPRNVYGMNQSEVYRNVDREDPKAILNGVAVTGLVGVLRQLGDLAEFAAEIFHGIQEEVMATASRSNQLKIRLQHIEATVPPLEKAMLAQTTHIHFAYTGGLEWHPRIPITQNHLIYDDLPHIIMDPYEECRGPPRLHLLDKFDINGPGSCLKRYSDPTYFRRASSNLSQGNKKFQKDKKHCKMKKKKTSSRSRDMSRLASLANQNARKTFASFSFSGQTSSTKTTSTSDMEKRYDFQDHHSRSFESRSGSGYNECLSTATSSLKTGERPKGVFVSSSLTPGSCTIASVLSECETEDAHDNFQFSPSQGQAARGSSCVSWDEKAEIVESLGLQTDEASEMVEANSVVDTLDEKPSYGEGIGGVDFHSKDNENDKSESGLRKRAGIDEVREIKNGREIVGEPRDSEQETESEGECFVDALNTIESESENNQGLQTSQVSSSCGVADERLEKSVCEQETEQNSYSVEDSCRSMDGLMANSFKNEENASSENVSVEMHQQNLQAGSDINRLQKNDLCANKDMRNDSGGKDTITFTFVPGLENSLVDSSNPLIHHGLQENQETEAESSGDLEAFKIWTNGGLLGLKPSKPPVLAMPSSLSPDCKTEERTVGFAEAEKDKADDLVENASHRHVLNNSSLATPGTQNPGSSNGIVMGIVDQRESHETSSGVFGLSHKFLTSGFRRKDSFAHDRKTVPATIPENDEVTTERRRFCDQDINEKTFMDPFRDEAPIDWITSSPPLQHMKISLNPADTLQASRLKLKFSDGDNTYNTFSSFQLLPETGTSLPDSYSDDDTFCRSSPYMSDTDYLSDNHSLSNSEPWEESSDSHGRKEQELYDSFHESRHVDNNAEASPLGIKSESSCVAVNLSYLQNPAEPLPPPFPPMQWMVSKTPSEKMEDKTQSLQLQEALRFAFEKHISLPTAKNELPSMVTSAPKPEIKAHLKNNVREEKQSANAKETETGDFLQQIRTQQFNLRPVVMTTTSSATATTDPIINTKISAILEKANSIRQAVASKDGDESDTWSDT 8082F565A5AA3FD0 brk1_arath Protein BRICK 1 BRK1 T9I22.8 At2g22640 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAKAGGITNAVNVGIAVQADWENREFISHISLNVRRLFEFLVQFESTTKSKLASLNEKLDLLERRLEMLEVQVSTATANPSLFAT CEF8D18BEB4B761C scar1_arath Protein SCAR1 Protein WAVE1 SCAR1 T14G11.27 At2g34150 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MPLVRLQVRNVYGLGQKELHTKVDREDPKAILDDVAVSGLVGILRQLGDLTEFAAEIFHGIQEEVMITASRSNKLKMRLKQIEAKVPTIQKTVLAQTNHIHFAYTGGLEWHPRIPNVQNHFMYDELPPFIMTPYEDCREPPRLHLLDKFDINGPGSCLKRYSDPTHFKRASRASKPSEIKKKKSIQRGRDISRLASVANQSDRKTCTSLSFSGRTSTSKTASTIEIESKSDLQEHRSFSFDSRSGGEKPKRVSSSSRFTPGSRTIASVLSESESESDSPSQDLTARGSSSVSWHEKAEIVECNVLQCATDEAPEVMETNFVLDAEPVSRLKEHSAVEAVQDIKPKELEMDNEDETESEGDDFVDALYTIDSESENDEAFQATKEVQKNLYNDITEQETEKISNNFSVDETKCAATSELHLSSSPVYKSDELIHQDPWAASEISSGTHSYSNGFSNPLYDISGIQEHQESEEVESSCDTESIKTWTNGNLLGLKPSKPKIIAETIPEIVEDIDSETFQEHLREDYKAPFDWFTSSPPLDHMKISFKSSETLPSSELQLKLPDEYTFSSFQLVPETIATSLPDSDSDKDTFCRSSSYISDNSDNDNRSVSMSEQQWEEESEGIRESKRQQELYDSFHRVNAEASSLPVPFPKIETTNGCLVENVSYLQNPAEPLPPPLPPLQWMVSKIPSAGFEDNNKQSLKDALTQAFEKNNNSLTAVKKKEPHIVTVSDPKLVTKVHLKNNVRDYKQSHGNTNETEAGDFLHQIRTKQFNLRRVVRTKTSSSETTMNTNISVILEKANSIRQAVASDDGEGESDTWSDSDT 67DB3E28A60F6115 brick1-scar1 1 4 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 3 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 4B As illustrated in Fig. 4B, using the same approach described for the full-length proteins, we found that the SHDs of AtSCAR3 and AtSCAR1 (AtSCAR3-SHD and AtSCAR1-SHD) bound 5-fold more efficiently to T7-tagged AtBRK1 than to the T7-tagged NC proteins. brick1-scar3 1 3 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 2 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction 4B As illustrated in Fig. 4B, using the same approach described for the full-length proteins, we found that the SHDs of AtSCAR3 and AtSCAR1 (AtSCAR3-SHD and AtSCAR1-SHD) bound 5-fold more efficiently to T7-tagged AtBRK1 than to the T7-tagged NC proteins.