UniProt UniProt http://www.uniprot.org Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2. Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2. Mol. Cell. Biol. 24 (9487-9497) 2004 imex curation Liu S., Milne GT., Kuremsky JG., Fink GR., Leppla SH. gfink@wi.mit.edu 22-Jan-2008: Contacted by JYOTI. Only protein-protein interactions crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Liu S., Milne GT., Kuremsky JG., Fink GR., Leppla SH. gfink@wi.mit.edu Accepted 2007-Not a month!-18 by CDEROW. Mol. Cell. Biol. 24 (9487-9497) 2004 Only protein-protein interactions imex curation dph2_mouse 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 MmDph2 Dph2 Dph2l2 Diphthamide biosynthesis protein 2 Diphtheria toxin resistance protein 2 S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase DPH2 protein protein mouse Mus musculus Mouse MESTFSSPAEAALQREAGVPGQFTPPEDLDRVYELERVTKFVCDLGCQRVTLQFPDQLLGDAGAVAARLEEVTGAKMFILGDTAYGSCCVDVLGAEQAGAQALVHFGPACLSPPASQLPITFVLGQRPVALELCAKAFEAQNPDPTAPVVLLSEPACAHALEPLAMLLLPKYQDLLISRPALPLPVGSPSSQPESLERFGRCFPLNPGRRLEEYGAFYVGASQASSDSSLDPDLSRLLLGWTPGRPFFSCCPDTGQTQDQGAKAGRLRARRLYLIERARDARVVGLLAGTLGVARHREALAHLRKLTEAAGKRSYVLAVGKPTPAKLANFPEMDVFVLLACPLGALAPQPSGGFFRPVLTPCELEAACNPAWPPPGLAPHLTHYAELLPGSPFHVPLPPPESELWDTPDVSLISGELRPPPPWKSSDDTRCSALIPRPQLELAESSPAASFLSSRNWQGLEPRLGQTPVKEAVRGRRGIAIAYEDEGSS 2D94A8FEDE63DD39 dph1_mouse 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 Ovarian cancer-associated gene 1 protein homolog Dph1 Dph2l1 Ovca1 Diphthamide biosynthesis protein 1 Diphtheria toxin resistance protein 1 S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 1 protein protein mouse Mus musculus Mouse MAALVVSETAEPGSRVGPGRGRISRGRLANQIPPEVLNNPQLQAAVQVLPSNYNFEIPKTIWRIQQAQAKKVALQMPEGLLLFACTIVDILERFTEAEVMVMGDVTYGACCVDDFTARALGVDFLVHYGHSCLVPMDTSVQDFRVLYVFVDIRIDTAHLLDSVRLTFTPGSSLALVSTIQFVSTLQAAAQELKADYHISVPQCKPLSPGEILGCTSPRLSKEVEAVVYLGDGRFHLESVMIANPNIPAYRYDPYGKVLSREYYDHQRMQATRQEAIAAARSAKSWGLILGTLGRQGSPKILEHLESQLRNLGLPFVRLLLSEIFPSKLSLLPEVDVWVQVACPRLSIDWGSAFPKPLLTPYEAAVALKDISWQQPYPMDFYSGSSLGPWTVNYGRDRAPRGLCQPASDKVQQGSRGGSPAPACESCNCADQKATSPAP 07CA505B08F72AB7 dph1-dph2 1 2 unspecified role unspecified role prey prey n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 3 unspecified role unspecified role bait bait n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 3D The results showed that MmDph2 could be specifically coimmunoprecipitated with MmDph1 and vice versa dph1-dph2-1 1 2 unspecified role unspecified role bait bait n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 3 unspecified role unspecified role prey prey n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 3D The results showed that MmDph2 could be specifically coimmunoprecipitated with MmDph1 and vice versa