IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Oncogene (0950-9232) 2004 imex curation Wiederhold T., Lee MF., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella JF., Ramesh V. Source of Merlin (P35240) determined from sequence length ramesh@helix.mgh.harvard.edu 10-APR-2007: Contacted by JYOTI. Human fetal frontel cortex Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wiederhold T., Lee MF., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella JF., Ramesh V. Source of Merlin (P35240) determined from sequence length ramesh@helix.mgh.harvard.edu Accepted 2007-APR-20 by JYOTI. 10-APR-2007: Contacted by JYOTI. Oncogene (0950-9232) 2004 Only protein-protein interactions imex curation Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Oncogene (0950-9232) 2004 imex curation Wiederhold T., Lee MF., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella JF., Ramesh V. Source of Merlin (P35240) determined from sequence length ramesh@helix.mgh.harvard.edu 10-APR-2007: Contacted by JYOTI. Human fetal frontel cortex Only protein-protein interactions human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wiederhold T., Lee MF., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella JF., Ramesh V. Source of Merlin (P35240) determined from sequence length ramesh@helix.mgh.harvard.edu Accepted 2007-APR-20 by JYOTI. 10-APR-2007: Contacted by JYOTI. Oncogene (0950-9232) 2004 Only protein-protein interactions imex curation Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Oncogene (0950-9232) 2004 imex curation Wiederhold T., Lee MF., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella JF., Ramesh V. Source of Merlin (P35240) determined from sequence length ramesh@helix.mgh.harvard.edu 10-APR-2007: Contacted by JYOTI. Human fetal frontel cortex Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Wiederhold T., Lee MF., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella JF., Ramesh V. Source of Merlin (P35240) determined from sequence length ramesh@helix.mgh.harvard.edu Accepted 2007-APR-20 by JYOTI. 10-APR-2007: Contacted by JYOTI. Human fetal frontel cortex Oncogene (0950-9232) 2004 Only protein-protein interactions imex curation Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Oncogene (0950-9232) 2004 imex curation Wiederhold T., Lee MF., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella JF., Ramesh V. Source of Merlin (P35240) determined from sequence length ramesh@helix.mgh.harvard.edu 10-APR-2007: Contacted by JYOTI. Human fetal frontel cortex Only protein-protein interactions mouse-cad Mouse neuronal cell line cad Mouse neuronal cell line electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy immunostaining immunostaining Wiederhold T., Lee MF., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella JF., Ramesh V. ramesh@helix.mgh.harvard.edu Accepted 2007-APR-20 by JYOTI. 10-APR-2007: Contacted by JYOTI. Oncogene (0950-9232) 2004 Only protein-protein interactions imex curation Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Oncogene (0950-9232) 2004 imex curation Wiederhold T., Lee MF., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella JF., Ramesh V. Source of Merlin (P35240) determined from sequence length ramesh@helix.mgh.harvard.edu 10-APR-2007: Contacted by JYOTI. Human fetal frontel cortex Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Wiederhold T., Lee MF., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella JF., Ramesh V. Additional information on Grb2 taken from manufacturers website ramesh@helix.mgh.harvard.edu Accepted 2007-APR-20 by JYOTI. 10-APR-2007: Contacted by JYOTI. Oncogene (0950-9232) 2004 Only protein-protein interactions imex curation Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Oncogene (0950-9232) 2004 imex curation Wiederhold T., Lee MF., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella JF., Ramesh V. Source of Merlin (P35240) determined from sequence length ramesh@helix.mgh.harvard.edu 10-APR-2007: Contacted by JYOTI. Human fetal frontel cortex Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Wiederhold T., Lee MF., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella JF., Ramesh V. ramesh@helix.mgh.harvard.edu Accepted 2007-APR-20 by JYOTI. 10-APR-2007: Contacted by JYOTI. Oncogene (0950-9232) 2004 Only protein-protein interactions imex curation Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Oncogene (0950-9232) 2004 imex curation Wiederhold T., Lee MF., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella JF., Ramesh V. Source of Merlin (P35240) determined from sequence length ramesh@helix.mgh.harvard.edu 10-APR-2007: Contacted by JYOTI. Human fetal frontel cortex Only protein-protein interactions mouse-cad Mouse neuronal cell line cad Mouse neuronal cell line fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Wiederhold T., Lee MF., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella JF., Ramesh V. ramesh@helix.mgh.harvard.edu Accepted 2007-APR-20 by JYOTI. 10-APR-2007: Contacted by JYOTI. Oncogene (0950-9232) 2004 Only protein-protein interactions imex curation Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Oncogene (0950-9232) 2004 imex curation Wiederhold T., Lee MF., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella JF., Ramesh V. Source of Merlin (P35240) determined from sequence length ramesh@helix.mgh.harvard.edu 10-APR-2007: Contacted by JYOTI. Human fetal frontel cortex Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Wiederhold T., Lee MF., James M., Neujahr R., Smith N., Murthy A., Hartwig J., Gusella JF., Ramesh V. Source of Merlin (P35240) determined from sequence length ramesh@helix.mgh.harvard.edu Accepted 2007-APR-23 by JYOTI. 10-APR-2007: Contacted by JYOTI. Oncogene (0950-9232) 2004 Only protein-protein interactions imex curation p35240-2 Merlin NF2 SCH Moesin-ezrin-radixin-like protein Neurofibromin-2 Schwannomerlin Schwannomin protein protein human Homo sapiens Human MAGAIASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKIYCPPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFAIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPAPLPPDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHNENSDRGGSSKHNTIKKSSPRQKTYLHLSPQSRLFPGTLYVVMLYVVMVLPSVILTRA 6A462A3A113A6C28 merl_mouse Merlin Moesin-ezrin-radixin-like protein Neurofibromin-2 Schwannomin Nf2 Nf-2 protein protein mouse Mus musculus Mouse MAGAIASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKVYCPPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFTIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPPPLPPDIPSFDIIADSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDVLHSESSDRGGPSSKHNTIKKLTLQSAKSRVAFFEEL 8D06F557E3435851 grb2_mouse Growth factor receptor-bound protein 2 Grb2 Adapter protein GRB2 SH2/SH3 adapter GRB2 protein protein mouse Mus musculus Mouse MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQMPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV 97F4A4FE4B248DDF grb2_human Growth factor receptor-bound protein 2 GRB2 ASH Adapter protein GRB2 SH2/SH3 adapter GRB2 Protein Ash protein protein human Homo sapiens Human MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV 83A4B0BA1B248DC4 med28_human Mediator of RNA polymerase II transcription subunit 28 Mediator complex subunit 28 Tumor angiogenesis marker EG-1 Endothelial-derived protein 1 Merlin and Grb2-interacting cytoskeletal protein MED28 EG1 FKSG20 protein protein human Homo sapiens Human MAAPLGGMFSGQPPGPPQAPPGLPGQASLLQAAPGAPRPSSSTLVDELESSFEACFASLVSQDYVNGTDQEEIRTGVDQCIQKFLDIARQTECFFLQKRLQLSVQKPEQVIKEDVSELRNELQRKDALVQKHLTKLRHWQQVLEDINVQHKKPADIPQGSLAYLEQASANIPAPLKPT 63DE023972908066 p35240-3 Merlin II NF2 SCH Moesin-ezrin-radixin-like protein Neurofibromin-2 Schwannomerlin Schwannomin protein protein human Homo sapiens Human MAGAIASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKIYCPPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFAIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPAPLPPDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHNENSDRGGSSKHNTIKKPQAQGRRPICI 99B732B48367D22A p35240-1 Merlin I NF2 SCH Moesin-ezrin-radixin-like protein Neurofibromin-2 Schwannomerlin Schwannomin protein protein human Homo sapiens Human MAGAIASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKIYCPPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFAIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPAPLPPDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHNENSDRGGSSKHNTIKKLTLQSAKSRVAFFEEL DIP protein P35240-1 original sequence version: 177 B1A1BF2BD5DA561C merl_human Merlin Moesin-ezrin-radixin-like protein Neurofibromin-2 Schwannomin Schwannomerlin NF2 SCH protein protein human Homo sapiens Human MAGAIASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKIYCPPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFAIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPAPLPPDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHNENSDRGGSSKHNTIKKLTLQSAKSRVAFFEEL B1A1BF2BD5DA561C nhrf1_human Na(+)/H(+) exchange regulatory cofactor NHE-RF1 SLC9A3R1 NHERF NHERF1 Ezrin-radixin-moesin-binding phosphoprotein 50 Regulatory cofactor of Na(+)/H(+) exchanger Sodium-hydrogen exchanger regulatory factor 1 Solute carrier family 9 isoform A3 regulatory factor 1 protein protein human Homo sapiens Human MSADAAAGAPLPRLCCLEKGPNGYGFHLHGEKGKLGQYIRLVEPGSPAEKAGLLAGDRLVEVNGENVEKETHQQVVSRIRAALNAVRLLVVDPETDEQLQKLGVQVREELLRAQEAPGQAEPPAAAEVQGAGNENEPREADKSHPEQRELRPRLCTMKKGPSGYGFNLHSDKSKPGQFIRSVDPDSPAEASGLRAQDRIVEVNGVCMEGKQHGDVVSAIRAGGDETKLLVVDRETDEFFKKCRVIPSQEHLNGPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL E33AF87016D37A65 med28_mouse Mediator of RNA polymerase II transcription subunit 28 Med28 Fksg20 Mediator complex subunit 28 protein protein mouse Mus musculus Mouse MAASLGGMFTGQPPGPPPPPPGLPGQASLLQAAPGAPRPSNSTLVDELESSFEACFASLVSQDYVNGTDQEEIRTGVDQCIQKFLDIARQTECFFLQKRLQLSVQKPDQVIKEDVSELRSELQRKDALVQKHLTKLRHWQQVLEDINVQHKKPADMPQGSLAFLEQASANIPAPLKQT 947FDA98E806EA13 nf2-med28-1 3 13 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait c-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association 1A, 1B Isolation of merlin binding proteins using the yeast two-hybrid system nf2-slc9a3r1 3 16 unspecified role unspecified role bait bait n-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role prey prey physical association physical association 1A, 1B Isolation of merlin binding proteins using the yeast two-hybrid system nf2-med28-2 8 13 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 16 unspecified role unspecified role bait bait c-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 29 32 physical association physical association 2 NP40 lysates from COS-7 cells overexpressing HA-merlintA were incubated with various GST-merlin fusion proteins immobilized on glutathione Sepharose 4B beads. nf2-med28-3 8 15 unspecified role unspecified role bait bait c-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 36 39 physical association physical association 2 NP40 lysates from COS-7 cells overexpressing HA-merlintA were incubated with various GST-merlin fusion proteins immobilized on glutathione Sepharose 4B beads. nf2-med28-4 2 16 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey physical association physical association 4A Magicin binds to merlin in vivo nf2-med28-5 2 13 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey physical association physical association 4A Magicin binds to merlin in vivo nf2-med28-6 8 9 unspecified role unspecified role bait bait c-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 52 49 physical association physical association 2 NP40 lysates from COS-7 cells overexpressing HA-merlintA were incubated with various GST-merlin fusion proteins immobilized on glutathione Sepharose 4B beads. nf2-med28-7 1 14 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P35240-3:p.Ser518Ala mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:50:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser518ala' Tue Jun 30 17:48:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser518Ala' ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association 4B Magicin exhibits enhanced binding with S518A than S518D for both merlin isoforms 1 or 2 nf2-med28-8 1 15 unspecified role unspecified role bait bait P35240-1:p.Ser518Ala mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:46:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser518ala' Tue Jun 30 16:08:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser518Ala' ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association 4B Magicin exhibits enhanced binding with S518A than "518D for both merlin isoforms 1 or 2 nf2-med28-9 7 13 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role neutral component neutral component P35240-3:p.Ser518Ala mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:00:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser518ala' Tue Jun 30 16:44:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser518Ala' region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) colocalization colocalization 5 Colocalization of magician with merlin and actin Magicin also colocalizes with actin but this could not be linked to a specific gene product med28-nf2-1 4 18 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization 6 Colocalization of merlin and magicin in the cortex of a CAD cell determined by electron microscopy. med28-grb2-1 6 11 unspecified role unspecified role prey prey mouse-cad Mouse neuronal cell line cad Mouse neuronal cell line 13 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association NP-40 lysates from CAD cells were incubated with GST or GST-magicin fusion proteins and bound proteins were detected with an anti-Grb2 antibody. 7A med28-grb2-2 2 13 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role prey prey physical association physical association Magicin and Grb2 co-immunoprecipitate. 7B Co-immunoprecipitates performed on heavey membrane/cytosolic fractions med28-grb2-3 5 13 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait sh3 domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain range range sh3 domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Blot overlay assays were then employed to test a direct interaction between magicin and Grb2 7D