IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The ErbB2/Neu/HER2 receptor is a new calmodulin-binding protein. The ErbB2/Neu/HER2 receptor is a new calmodulin-binding protein. Biochem. J. (0264-6021) 2004 imex curation Li H., Sanchez-Torres J., Del Carpio A., Salas V., Villalobo A. Origin of P62157 taken from manufacturer's web site antonio.villalobo@iib.uam.es 29-OCT-2007: Contacted by JYOTI. 2007-06-01: Antonio Villalobo, Instituto de Investigaciones Biomedicas, Universidad Autonoma de Madrid, Spain Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Li H., Sanchez-Torres J., Del Carpio A., Salas V., Villalobo A. Origin of P62157 taken from manufacturer's web site antonio.villalobo@iib.uam.es Accepted 2007-JUL-14 by JYOTI. 2007-06-01: Antonio Villalobo, Instituto de Investigaciones Biomedicas, Universidad Autonoma de Madrid, Spain Biochem. J. (0264-6021) 2004 Only protein-protein interactions imex curation The ErbB2/Neu/HER2 receptor is a new calmodulin-binding protein. The ErbB2/Neu/HER2 receptor is a new calmodulin-binding protein. Biochem. J. (0264-6021) 2004 imex curation Li H., Sanchez-Torres J., Del Carpio A., Salas V., Villalobo A. Origin of P62157 taken from manufacturer's web site antonio.villalobo@iib.uam.es 29-OCT-2007: Contacted by JYOTI. 2007-06-01: Antonio Villalobo, Instituto de Investigaciones Biomedicas, Universidad Autonoma de Madrid, Spain Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Li H., Sanchez-Torres J., Del Carpio A., Salas V., Villalobo A. Prey proteins initially visualised by development with streptavidin-peroxidase, presence of ERBB2 then confirmed by western blot. Origin of P62157 taken from manufacturer's web site antonio.villalobo@iib.uam.es Accepted 2007-JUL-14 by JYOTI. 29-OCT-2007: Contacted by JYOTI. 2007-06-01: Antonio Villalobo, Instituto de Investigaciones Biomedicas, Universidad Autonoma de Madrid, Spain Biochem. J. (0264-6021) 2004 Only protein-protein interactions imex curation erbb2_human Receptor tyrosine-protein kinase erbB-2 p185erbB2 MLN19 Proto-oncogene c-ErbB-2 Tyrosine kinase-type cell surface receptor HER2 Proto-oncogene Neu ERBB2 HER2 NEU NGL Metastatic lymph node gene 19 protein protein protein human Homo sapiens Human MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV DIP protein P04626 original sequence version: 198 DIP protein P04626 original sequence version: 122 DIP protein P04626 original sequence version: 206 DIP protein P04626 original sequence version: 195 DIP protein P04626 original sequence version: 150 DIP protein P04626 original sequence version: 179 DIP protein P04626 original sequence version: 146 DIP protein P04626 original sequence version: 147 39E9DFDA04DCF962 calm_rat Calmodulin Calm1 Calm Cam Cam1 Calm2 Cam2 Camb Calm3 Cam3 Camc CaMII protein protein rat Rattus norvegicus (Rat) Rat MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK DIP protein P62161 original sequence version: 125 DIP protein P62161 original sequence version: 51 DIP protein P62161 original sequence version: 55 DIP protein P62161 original sequence version: 59 DIP protein P62161 original sequence version: 80 DIP protein P62161 original sequence version: 81 DIP protein P62161 original sequence version: 132 DIP protein P62161 original sequence version: 96 6B4BC3FCDE10727B calm_bovin Calmodulin CALM CAM protein protein bovin Bos taurus (Bovine) Bovine MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK DIP protein P62157 original sequence version: 125 DIP protein P62157 original sequence version: 58 DIP protein P62157 original sequence version: null 6B4BC3FCDE10727B calm-erbb2-1 1 3 unspecified role unspecified role prey prey human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion 5 unspecified role unspecified role bait bait bovin-brain Bos taurus brain brain Brain [cerebrum] physical association physical association To determined whether or not ErbB2 could be isolated by CaM-affinity binding, we performed pull-down experiments using CaM-agarose. Fig. 4 Interaction is calcium dependent. The presence of the CaM antagonist W7 (N-(6-aminohexyl)-5-chloro-1-naphthalenesulphonamide) increases the binding of ErbB2 to CaM�agarose beads. calm-erbb2-2 2 3 unspecified role unspecified role prey prey human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion 4 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction To determine whether ErbB2 could be responsible at least in part for the high-molecular-mass signal observed in overlay experiments with biotinylated CaM using whole cell extracts, we repeated the experiment using immunoprecipitated ErbB2. Fig. 6 Interaction is calcium dependent A 160kDa protein was also observed to bind to calmodulin