UniProt UniProt http://www.uniprot.org P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. Mol. Biol. Cell (1059-1524) 2004 imex curation Di Stefano P., Cabodi S., Boeri Erba E., Margaria V., Bergatto E., Giuffrida MG., Silengo L., Tarone G., Turco E., Defilippi P. Lysate of human ECV304 cells has been used. paola.defilippi@unito.it 13-Nov-2007: Contacted by JYOTI. orchard Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Di Stefano P., Cabodi S., Boeri Erba E., Margaria V., Bergatto E., Giuffrida MG., Silengo L., Tarone G., Turco E., Defilippi P. Lysate of human ECV304 cells has been used. paola.defilippi@unito.it Accepted 2007-NOV-3 by ORCHARD. 13-Nov-2007: Contacted by JYOTI. Mol. Biol. Cell (1059-1524) 2004 orchard Only protein-protein interactions imex curation P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. Mol. Biol. Cell (1059-1524) 2004 imex curation Di Stefano P., Cabodi S., Boeri Erba E., Margaria V., Bergatto E., Giuffrida MG., Silengo L., Tarone G., Turco E., Defilippi P. Lysate of human ECV304 cells has been used. paola.defilippi@unito.it 13-Nov-2007: Contacted by JYOTI. orchard Only protein-protein interactions human-ecv304 Human umbilical cord, endothelial ECV304 ecv304 Human umbilical cord, endothelial ECV304 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Di Stefano P., Cabodi S., Boeri Erba E., Margaria V., Bergatto E., Giuffrida MG., Silengo L., Tarone G., Turco E., Defilippi P. Lysate of human ECV304 cells has been used. paola.defilippi@unito.it Accepted 2007-NOV-3 by ORCHARD. 13-Nov-2007: Contacted by JYOTI. Mol. Biol. Cell (1059-1524) 2004 orchard Only protein-protein interactions imex curation P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. Mol. Biol. Cell (1059-1524) 2004 imex curation Di Stefano P., Cabodi S., Boeri Erba E., Margaria V., Bergatto E., Giuffrida MG., Silengo L., Tarone G., Turco E., Defilippi P. Lysate of human ECV304 cells has been used. paola.defilippi@unito.it 13-Nov-2007: Contacted by JYOTI. orchard Only protein-protein interactions human-ecv304 Human umbilical cord, endothelial ECV304 ecv304 Human umbilical cord, endothelial ECV304 confocal microscopy confocal microscopy immunostaining immunostaining Di Stefano P., Cabodi S., Boeri Erba E., Margaria V., Bergatto E., Giuffrida MG., Silengo L., Tarone G., Turco E., Defilippi P. Lysate of human ECV304 cells has been used. paola.defilippi@unito.it Accepted 2007-NOV-3 by ORCHARD. 13-Nov-2007: Contacted by JYOTI. Mol. Biol. Cell (1059-1524) 2004 orchard Only protein-protein interactions imex curation P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. Mol. Biol. Cell (1059-1524) 2004 imex curation Di Stefano P., Cabodi S., Boeri Erba E., Margaria V., Bergatto E., Giuffrida MG., Silengo L., Tarone G., Turco E., Defilippi P. Lysate of human ECV304 cells has been used. paola.defilippi@unito.it 13-Nov-2007: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom fingerprinting peptide massfingerprinting fingerprinting Di Stefano P., Cabodi S., Boeri Erba E., Margaria V., Bergatto E., Giuffrida MG., Silengo L., Tarone G., Turco E., Defilippi P. Lysate of human ECV304 cells has been used. paola.defilippi@unito.it Accepted 2007-NOV-3 by ORCHARD. Mol. Biol. Cell (1059-1524) 2004 orchard Only protein-protein interactions imex curation P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. Mol. Biol. Cell (1059-1524) 2004 imex curation Di Stefano P., Cabodi S., Boeri Erba E., Margaria V., Bergatto E., Giuffrida MG., Silengo L., Tarone G., Turco E., Defilippi P. Lysate of human ECV304 cells has been used. paola.defilippi@unito.it 13-Nov-2007: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Di Stefano P., Cabodi S., Boeri Erba E., Margaria V., Bergatto E., Giuffrida MG., Silengo L., Tarone G., Turco E., Defilippi P. Lysate of human ECV304 cells has been used. paola.defilippi@unito.it Accepted 2007-NOV-3 by ORCHARD. 13-Nov-2007: Contacted by JYOTI. Mol. Biol. Cell (1059-1524) 2004 orchard Only protein-protein interactions imex curation P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. Mol. Biol. Cell (1059-1524) 2004 imex curation Di Stefano P., Cabodi S., Boeri Erba E., Margaria V., Bergatto E., Giuffrida MG., Silengo L., Tarone G., Turco E., Defilippi P. Lysate of human ECV304 cells has been used. paola.defilippi@unito.it 13-Nov-2007: Contacted by JYOTI. orchard Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip antibody detection identification by antibody antibody detection Di Stefano P., Cabodi S., Boeri Erba E., Margaria V., Bergatto E., Giuffrida MG., Silengo L., Tarone G., Turco E., Defilippi P. Lysate of human ECV304 cells has been used. paola.defilippi@unito.it Accepted 2007-NOV-3 by ORCHARD. 13-Nov-2007: Contacted by JYOTI. Mol. Biol. Cell (1059-1524) 2004 orchard Only protein-protein interactions imex curation srcn1_mouse SRC kinase signaling inhibitor 1 p140Cap SNAP-25-interacting protein Srcin1 Kiaa1684 P140 p130Cas-associated protein protein protein mouse Mus musculus Mouse MQPWQCLRRFALAWWERTAEGRARSPREEVGPRDPGGRGEPDPERSSPPMLSADDAEYPREYRTLGGGGGGGSGGRRFSNVGLVHTSERRHTVIAAQSLEALSGLQKADADRKRDAFMDHLKSKYPQHALALRGQQDRMREQVGGWTVDPVCLLSSLCSHLHGDSTPSGAGQPAQQPNYWSFKTRSSRHTQGAQPGLADQAAKLSYASAESLETMSEAELPLGFSRMNRFRQSLPLSRSASQTKLRSPGVLFLQFGEETRRVHITHEVSSLDTLHALIAHMFPQKLTMGMLKSPNTAILIKDEARNVFYELEDVRDIQDRSIIKIYRKEPLYAAFPGSHLTNGDLRREMVYASRESSPTRRLNNLSPASHLASSSPPPGLPSGLPSGLPSGSPSRSRLSYAGGRPPSYAGSPVHHAAERLGGAPTGQGVSPSPSAILERRDVKPDEDLAGKAGGMVLVKGEGLYADPYGLLHEGRLSLAAAAETHSHTRARAACTSGVPCALSAPTPLPRCSPTWRTRCTRRALAALYGDGYGFRLPPSSPQKLADVSAPSGGPPPPHSPYSGPPSRGSPVRQSFRKDSGSSSVFAESPGGKARSTGSASTAGAPPSELFPGPGERSLVGFGPPVPAKDTETRERMEAMEKQIASLTGLVQSALLRGSEPETPSEKVEGSNGAATPSAPVCGSGSKSSGATPVSGPPPPSASSTPAGQPTAVSRLQMQLHLRGLQNSASDLRGQLQQLRNVQLQNQESVRALLKPTEADVSMRVSEAARRQEDPLQRQRTLVEEERLRYLNDEELITQQLNDLEKSVEKIQRDVAHNHRLMPGPELEEKALVLKQLGETLTELKAHFPGLQSKMRVVLRVEVEAVKFLKEEPQRLDGLLKRCRGVTDTLAQIRRQVDEGMWPPPNNLLNQSPKKVAAETDFSKGLDFEIPPPSPPLNLHELSGPAEGTPLTPKSTNPTKCLDASSKRNTDKAVSVEAAERDWEEKRAALTQYSAKDINRLLEETQAELLKAIPDLDCASKTHPGPAPTPDHKPPKAPHGQKAAPRTEPSGRRGSDELTVPRYRTEKPSKSPPPPPPRRSFPSSHGLTTTRTGEVVVTSKKDSVFIKKAESEELEVQKPQVKLRRAVSEVVRPASTPPIMASAIKDEDDEERIIAELESGGSSVPPMKVVTPGASRLKAAQGPAGSPDKGKHGKQRTEYMRIQAQQQATKPSKEVSGPNETSSPGSEKPSGSRTSIPVLTSFGARNSSISF 85F394C9523F78C0 bcar1_human Breast cancer anti-estrogen resistance protein 1 CRK-associated substrate p130cas BCAR1 CAS CRKAS CASS1 Cas scaffolding protein family member 1 protein protein human Homo sapiens Human MNHLNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPAGPGPGPPATPAQPQPGLHAPAPPASQYTPMLPNTYQPQPDSVYLVPTPSKAQQGLYQVPGPSPQFQSPPAKQTSTFSKQTPHHPFPSPATDLYQVPPGPGGPAQDIYQVPPSAGMGHDIYQVPPSMDTRSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYDVPPSVEKGLPPSNHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPPPDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREAPAEGKRLSASSTGSTRSSQSASSLEVAGPGREPLELEVAVEALARLQQGVSATVAHLLDLAGSAGATGSWRSPSEPQEPLVQDLQAAVAAVQSAVHELLEFARSAVGNAAHTSDRALHAKLSRQLQKMEDVHQTLVAHGQALDAGRGGSGATLEDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKATAPGPEGGGTLHPNPTDKTSSIQSRPLPSPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGKEEFEKTQKELLEKGSITRQGKSQLELQQLKQFERLEQEVSRPIDHDLANWTPAQPLAPGRTGGLGPSDRQLLLFYLEQCEANLTTLTNAVDAFFTAVATNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSPSAAQDMVERVKELGHSTQQFRRVLGQLAAA DIP protein P56945 original sequence version: 113 DIP protein P56945 original sequence version: 116 B81EC3430049E795 crk_human Adapter molecule crk Proto-oncogene c-Crk CRK p38 protein protein human Homo sapiens Human MAGNFDSEERSSWYWGRLSRQEAVALLQGQRHGVFLVRDSSTSPGDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPALLEFYKIHYLDTTTLIEPVSRSRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDILRIRDKPEEQWWNAEDSEGKRGMIPVPYVEKYRPASASVSALIGGNQEGSHPQPLGGPEPGPYAQPSVNTPLPNLQNGPIYARVIQKRVPNAYDKTALALEVGELVKVTKINVSGQWEGECNGKRGHFPFTHVRLLDQQNPDEDFS 4CFBFB65BFC2E265 srcn1_human SRC kinase signaling inhibitor 1 SRCIN1 KIAA1684 P140 p130Cas-associated protein p140Cap SNAP-25-interacting protein SNIP protein protein human Homo sapiens Human MGNAPSQDPERSSPPMLSADDAEYPREYRTLGGGGGGGSGGRRFSNVGLVHTSERRHTVIAAQSLEALSGLQKADADRKRDAFMDHLKSKYPQHALALRGQQDRMREQPNYWSFKTRSSRHTQGAQPGLADQAAKLSYASAESLETMSEAELPLGFSRMNRFRQSLPLSRSASQTKLRSPGVLFLQFGEETRRVHITHEVSSLDTLHALIAHMFPQKLTMGMLKSPNTAILIKDEARNVFYELEDVRDIQDRSIIKIYRKEPLYAAFPGSHLTNGDLRREMVYASRESSPTRRLNNLSPAPHLASGSPPPGLPSGLPSGLQSGSPSRSRLSYAGGRPPSYAGSPVHHAAERLGGAPAAQGVSPSPSAILERRDVKPDEDLASKAGGMVLVKGEGLYADPYGLLHEGRLSLAAAAGDPFAYPGAGGLYKRGSVRSLSTYSAAALQSDLEDSLYKAAGGGGPLYGDGYGFRLPPSSPQKLADVAAPPGGPPPPHSPYSGPPSRGSPVRQSFRKDSGSSSVFAESPGGKTRSAGSASTAGAPPSELFPGPGERSLVGFGPPVPAKDTETRERMEAMEKQIASLTGLVQSALLRGSEPETPSEKIEGSNGAATPSAPCGSGGRSSGATPVSGPPPPSASSTPAGQPTAVSRLQMQLHLRGLQNSASDLRGQLQQLRKLQLQNQESVRALLKRTEAELSMRVSEAARRQEDPLQRQRTLVEEERLRYLNDEELITQQLNDLEKSVEKIQRDVSHNHRLVPGPELEEKALVLKQLGETLTELKAHFPGLQSKMRVVLRVEVEAVKFLKEEPQRLDGLLKRCRGVTDTLAQIRRQVDEGVWPPPNNLLSQSPKKVTAETDFNKSVDFEMPPPSPPLNLHELSGPAEGASLTPKGGNPTKGLDTPGKRSVDKAVSVEAAERDWEEKRAALTQYSAKDINRLLEETQAELLKAIPDLDCASKAHPGPAPTPDHKPPKAPHGQKAAPRTEPSGRRGSDELTVPRYRTEKPSKSPPPPPPRRSFPSSHGLTTTRTGEVVVTSKKDSAFIKKAESEELEVQKPQVKLRRAVSEVARPASTPPIMASAIKDEDDEDRIIAELESGGGSVPPMKVVTPGASRLKAAQGQAGSPDKSKHGKQRAEYMRIQAQQQATKPSKEMSGSNETSSPVSEKPSASRTSIPVLTSFGARNSSISF 52BF566E715EF6FF bcar1_mouse Breast cancer anti-estrogen resistance protein 1 Bcar1 Cas Crkas p130cas CRK-associated substrate protein protein mouse Mus musculus Mouse MTVPNVLAKALYDNVAESPDELSFRKGDIMTVLERDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPVGPGPGPPATPPQPQPSLPQGVHAPVPPASQYSPMLPTAYQPQSDNVYLVPTPSKTQQGLYQAPGPNPQFQSPPAKQTSTFSKQTPHHSFPSPATDLYQVPPGPGSPAQDIYQVPPSAGIGHDIYQVPPSLDTRGWEGTKPPAKVVVPTRVGQGYVYEAAQTEQDEYDTPRHLLAPGPQDIYDVPPVRGLLPNQYGQEVYDTPPMAVKGPNGRDPLLDVYDVPPSVEKGLLSSSHHSVYDVPPSVSKDVPDGPLLREETYDVPPAFAKPKPFDPTRHPLILAAPPPDSPAAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGSVVDDGVYAVPPPAEREAPTDGKRLSASSTGSTRSSQSASSLEVVVPGREPLELEVAVESLARLQQGVSTTVAHLLDLVGSASGPGGWRGTSEPQEPPAQDLKAAVAAVHGAVHELLEFARGAVSNATHTSDRTLHAKLSRQLQKMEDVYQTLVVHGQVLDSGRGSPGFTPEDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKAPGPGPEGSSSLHPNPTDKASSIQSRPLPSPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGKEEFEKTQKELLERGNIMRQGKGQLELQQLKQFERLEQEVSRPIDHDLANWTPAQPLVPGRTGGLGPSDRQLLLFYLEQCEANLTTLTDAVDAFFTAVATNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSPSAAQDMVDRVKELGHSTQQFRRVLGQLAAA 81E56BC7AD87B095 bcar1-p140-1 4 10 unspecified role unspecified role prey prey human-ecv304 Human umbilical cord, endothelial ECV304 ecv304 Human umbilical cord, endothelial ECV304 11 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 1B Therefore, these data show that the human unknown KIAA1684 protein corresponds to the p140 band selectively eluted from MBP-p130Cas column, suggesting that the two proteins might associate into the cells. bcar1-p140-2 2 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 4A As shown in Figure 4A, antibodies to p140Cap were able to immunoprecipitate p130Cas and vice versa, indicating that the endogenous proteins associate in an immunoprecipitable complex in ECV304 cells. p140-bcar1-1 2 10 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 4A As shown in Figure 4A, antibodies to p140Cap were able to immunoprecipitate p130Cas and vice versa, indicating that the endogenous proteins associate in an immunoprecipitable complex in ECV304 cells. p140-bcar1-2 6 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey association association 4B In addition p140Cap and p130Cas immunocomplexes also contained the adaptor protein Crk, a known interactor of p130Cas, indicating that p140Cap and p130Cas take part in a macromolecular complex. bcar1-p140-3 6 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey association association 4B In addition p140Cap and p130Cas immunocomplexes also contained the adaptor protein Crk, a known interactor of p130Cas, indicating that p140Cap and p130Cas take part in a macromolecular complex. p140-bcar1-3 5 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association 5A The C-terminal of SNIP as given in the UnIProt database is longer than stated in the paper, nor can this difference be ascribed to any of the known isoforms. bcar1-p140-5 1 7 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 8 unspecified role unspecified role bait bait physical association physical association 5C A P140 deletion construct missing the c-terminus (aa 1006-1250) did not interact. Positions of c-term different from SP. The protein in UniProt is longer at the C-terminus than described in the paper bcar1-p140-6 5 7 unspecified role unspecified role prey prey human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 5D p140-bcar1-7 3 10 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 6 Colocalization at cytoplasmic punctate structures. Upon treatment with PMA colocalization occurs at membrane ruffles. P140 is localized to actin cytoskeleton. Subcellular localization of p140Cap and colocalization with p130Cas. crk-p140-1 6 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 4B In addition p140Cap and p130Cas immunocomplexes also contained the adaptor protein Crk, a known interactor of p130Cas, indicating that p140Cap and p130Cas take part in a macromolecular complex.