IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ p250GAP, a neural RhoGAP protein, is associated with and phosphorylated by Fyn. p250GAP, a neural RhoGAP protein, is associated with and phosphorylated by Fyn. Biochem. Biophys. Res. Commun. (0006-291X) 2003 imex curation Taniguchi S., Liu H., Nakazawa T., Yokoyama K., Tezuka T., Yamamoto T. Source of Fyn taken from PMID: 8262983 tyamamot@ims.u-tokyo.ac.jp 10-APR-2007: Contacted by JYOTI. Human fetal brain cDNA Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Taniguchi S., Liu H., Nakazawa T., Yokoyama K., Tezuka T., Yamamoto T. Source of Fyn taken from PMID: 8262983 tyamamot@ims.u-tokyo.ac.jp Accepted 2007-APR-12 by DAVET. 10-APR-2007: Contacted by JYOTI. Human fetal brain cDNA Biochem. Biophys. Res. Commun. (0006-291X) 2003 Only protein-protein interactions imex curation p250GAP, a neural RhoGAP protein, is associated with and phosphorylated by Fyn. p250GAP, a neural RhoGAP protein, is associated with and phosphorylated by Fyn. Biochem. Biophys. Res. Commun. (0006-291X) 2003 imex curation Taniguchi S., Liu H., Nakazawa T., Yokoyama K., Tezuka T., Yamamoto T. Source of Fyn taken from PMID: 8262983 tyamamot@ims.u-tokyo.ac.jp 10-APR-2007: Contacted by JYOTI. Human fetal brain cDNA Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Taniguchi S., Liu H., Nakazawa T., Yokoyama K., Tezuka T., Yamamoto T. Source of Fyn taken from PMID: 8262983 tyamamot@ims.u-tokyo.ac.jp Accepted 2007-APR-12 by DAVET. 10-APR-2007: Contacted by JYOTI. Human fetal brain cDNA Biochem. Biophys. Res. Commun. (0006-291X) 2003 Only protein-protein interactions imex curation p250GAP, a neural RhoGAP protein, is associated with and phosphorylated by Fyn. p250GAP, a neural RhoGAP protein, is associated with and phosphorylated by Fyn. Biochem. Biophys. Res. Commun. (0006-291X) 2003 imex curation Taniguchi S., Liu H., Nakazawa T., Yokoyama K., Tezuka T., Yamamoto T. Source of Fyn taken from PMID: 8262983 tyamamot@ims.u-tokyo.ac.jp 10-APR-2007: Contacted by JYOTI. Human fetal brain cDNA Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Taniguchi S., Liu H., Nakazawa T., Yokoyama K., Tezuka T., Yamamoto T. Source of Fyn taken from PMID: 8262983 tyamamot@ims.u-tokyo.ac.jp Accepted 2007-APR-12 by DAVET. 10-APR-2007: Contacted by JYOTI. Human fetal brain cDNA Biochem. Biophys. Res. Commun. (0006-291X) 2003 Only protein-protein interactions imex curation p250GAP, a neural RhoGAP protein, is associated with and phosphorylated by Fyn. p250GAP, a neural RhoGAP protein, is associated with and phosphorylated by Fyn. Biochem. Biophys. Res. Commun. (0006-291X) 2003 imex curation Taniguchi S., Liu H., Nakazawa T., Yokoyama K., Tezuka T., Yamamoto T. Source of Fyn taken from PMID: 8262983 tyamamot@ims.u-tokyo.ac.jp 10-APR-2007: Contacted by JYOTI. Human fetal brain cDNA Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Taniguchi S., Liu H., Nakazawa T., Yokoyama K., Tezuka T., Yamamoto T. Source of Fyn taken from PMID: 8262983 tyamamot@ims.u-tokyo.ac.jp Accepted 2007-APR-12 by DAVET. 10-APR-2007: Contacted by JYOTI. Human fetal brain cDNA Biochem. Biophys. Res. Commun. (0006-291X) 2003 Only protein-protein interactions imex curation rhg32_human Rho GTPase-activating protein 32 ARHGAP32 GRIT KIAA0712 RICS Brain-specific Rho GTPase-activating protein GAB-associated Cdc42/Rac GTPase-activating protein GC-GAP GTPase regulator interacting with TrkA Rho-type GTPase-activating protein 32 Rho/Cdc42/Rac GTPase-activating protein RICS RhoGAP involved in the beta-catenin-N-cadherin and NMDA receptor signaling p200RhoGAP p250GAP protein protein human Homo sapiens Human METESESSTLGDDSVFWLESEVIIQVTDCEEEEREEKFRKMKSSVHSEEDDFVPELHRNVHPRERPDWEETLSAMARGADVPEIPGDLTLKTCGSTASMKVKHVKKLPFTKGHFPKMAECAHFHYENVEFGSIQLSLSEEQNEVMKNGCESKELVYLVQIACQGKSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDTLKDSPESVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINTPAVGAAHVIKRYTARAPDELTLEVGDIVSVIDMPPKVLSTWWRGKHGFQVGLFPGHCVELINQKVPQSVTNSVPKPVSKKHGKLITFLRTFMKSRPTKQKLKQRGILKERVFGCDLGEHLLNSGFEVPQVLQSCTAFIERYGIVDGIYRLSGVASNIQRLRHEFDSEHVPDLTKEPYVQDIHSVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSLLADYCSITNMHAKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSGRISMAMQEGAASLSRPKSLLVSSPSTKLLTLEEAQARTQAQVNSPIVTENKYIEVGEGPAALQGKFHTIIEFPLERKRPQNKMKKSPVGSWRSFFNLGKSSSVSKRKLQRNESEPSEMKAMALKGGRAEGTLRSAKSEESLTSLHAVDGDSKLFRPRRPRSSSDALSASFNGEMLGNRCNSYDNLPHDNESEEEGGLLHIPALMSPHSAEDVDLSPPDIGVASLDFDPMSFQCSPPKAESECLESGASFLDSPGYSKDKPSANKKDAETGSSQCQTPGSTASSEPVSPLQEKLSPFFTLDLSPTEDKSSKPSSFTEKVVYAFSPKIGRKLSKSPSMSISEPISVTLPPRVSEVIGTVSNTTAQNASSSTWDKCVEERDATNRSPTQIVKMKTNETVAQEAYESEVQPLDQVAAEEVELPGKEDQSVSSSQSKAVASGQTQTGAVTHDPPQDSVPVSSVSLIPPPPPPKNVARMLALALAESAQQASTQSLKRPGTSQAGYTNYGDIAVATTEDNLSSSYSAVALDKAYFQTDRPAEQFHLQNNAPGNCDHPLPETTATGDPTHSNTTESGEQHHQVDLTGNQPHQAYLSGDPEKARITSVPLDSEKSDDHVSFPEDQSGKNSMPTVSFLDQDQSPPRFYSGDQPPSYLGASVDKLHHPLEFADKSPTPPNLPSDKIYPPSGSPEENTSTATMTYMTTTPATAQMSTKEASWDVAEQPTTADFAAATLQRTHRTNRPLPPPPSQRSAEQPPVVGQVQAATNIGLNNSHKVQGVVPVPERPPEPRAMDDPASAFISDSGAAAAQCPMATAVQPGLPEKVRDGARVPLLHLRAESVPAHPCGFPAPLPPTRMMESKMIAAIHSSSADATSSSNYHSFVTASSTSVDDALPLPLPVPQPKHASQKTVYSSFARPDVTTEPFGPDNCLHFNMTPNCQYRPQSVPPHHNKLEQHQVYGARSEPPASMGLRYNTYVAPGRNASGHHSKPCSRVEYVSSLSSSVRNTCYPEDIPPYPTIRRVQSLHAPPSSMIRSVPISRTEVPPDDEPAYCPRPLYQYKPYQSSQARSDYHVTQLQPYFENGRVHYRYSPYSSSSSSYYSPDGALCDVDAYGTVQLRPLHRLPNRDFAFYNPRLQGKSLYSYAGLAPRPRANVTGYFSPNDHNVVSMPPAADVKHTYTSWDLEDMEKYRMQSIRRESRARQKVKGPVMSQYDNMTPAVQDDLGGIYVIHLRSKSDPGKTGLLSVAEGKESRHAAKAISPEGEDRFYRRHPEAEMDRAHHHGGHGSTQPEKPSLPQKQSSLRSRKLPDMGCSLPEHRAHQEASHRQFCESKNGPPYPQGAGQLDYGSKGIPDTSEPVSYHNSGVKYAASGQESLRLNHKEVRLSKEMERPWVRQPSAPEKHSRDCYKEEEHLTQSIVPPPKPERSHSLKLHHTQNVERDPSVLYQYQPHGKRQSSVTVVSQYDNLEDYHSLPQHQRGVFGGGGMGTYVPPGFPHPQSRTYATALGQGAFLPAELSLQHPETQIHAE 075E5B4902857D06 fyn_human Tyrosine-protein kinase Fyn p59-Fyn Proto-oncogene c-Fyn Src-like kinase FYN Proto-oncogene Syn protein protein human Homo sapiens Human MGCVQCKDKEATKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSERAAGLCCRLVVPCHKGMPRLTDLSVKTKDVWEIPRESLQLIKRLGNGQFGEVWMGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQSFLEDYFTATEPQYQPGENL DIP protein P06241 original sequence version: 195 DIP protein P06241 original sequence version: 147 DIP protein P06241 original sequence version: 164 4A1E443A4B5A0977 fyn-rics-1 4 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey c-terminal region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain physical association physical association Update to P06241-1 once logfile run in UniProt. To identify molecules that interact with Fyn, we performed yeast two-hybrid screening using full-length Fyn as a bait. fyn-rics-2 2 5 unspecified role unspecified role bait bait c-terminal region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey P06241:p.Tyr531Phe constitutively active mutation mutation certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 19:56:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr531phe' Tue Jun 30 17:12:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr531Phe' direct interaction direct interaction Update to P06241-1 once logfile run in UniProt. As shown in Fig. 1B, the GST-p250GAPTH2 fusion protein specifically precipitated a constitutively active form of Fyn (FynY531F), indicating that p250GAP interacts directly with Fyn in vitro. 1 fyn-rics-3 1 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey P06241:p.Lys299Met Catalytically inactive mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K M Wed May 02 17:02:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys299met' Tue Jun 30 17:23:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys299Met' P06241:p.Tyr531Phe Constitutively active mutation mutation certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 17:02:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr531phe' Tue Jun 30 18:45:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr531Phe' physical association physical association Update to P06241-1 once logfile run in UniProt. To determine whether p250GAP interacts with Fyn in vivo, we carried out co-immunoprecipitation analysis in HEK293T cells transfected with the expression plasmids for Flag-tagged p250GAP and/or Fyn (FynY531F). 2A fyn-rics-4 3 5 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait P06241:p.Lys299Met Catalytically inactive mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K M Wed May 02 15:24:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys299met' Tue Jun 30 20:13:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys299Met' P06241:p.Tyr531Phe Constitutively active mutation mutation certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 18:49:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr531phe' Tue Jun 30 16:01:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr531Phe' physical association physical association Update to P06241-1 once logfile run in UniProt. Similar results were obtained when anti-Fyn immunoprecipitates were probed with anti-p250GAP antibodies ( Fig. 3B). 3B