IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ PACE-1, a novel protein that interacts with the C-terminal domain of ezrin. PACE-1, a novel protein that interacts with the C-terminal domain of ezrin. Exp. Cell Res. (0014-4827) 2003 imex curation Sullivan A., Uff CR., Isacke CM., Thorne RF. The sequence for PACE-1 given by the authors corresponds to the sequence variations specified by Ref. 1 in the UniProtKb entry Q8IZE3. rthorne@icr.ac.uk 12-Oct-2007: Contacted by JYOTI. A human kidney Matchmaker cDNA library in the pGAD10 vector (Clontech) was used. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Sullivan A., Uff CR., Isacke CM., Thorne RF. The sequence for PACE-1 given by the authors corresponds to the sequence variations specified by Ref. 1 in the UniProtKb entry Q8IZE3. rthorne@icr.ac.uk Accepted 2007-OCT-4 by JYOTI. 12-Oct-2007: Contacted by JYOTI. A human kidney Matchmaker cDNA library in the pGAD10 vector (Clontech) was used. Exp. Cell Res. (0014-4827) 2003 Only protein-protein interactions imex curation PACE-1, a novel protein that interacts with the C-terminal domain of ezrin. PACE-1, a novel protein that interacts with the C-terminal domain of ezrin. Exp. Cell Res. (0014-4827) 2003 imex curation Sullivan A., Uff CR., Isacke CM., Thorne RF. The sequence for PACE-1 given by the authors corresponds to the sequence variations specified by Ref. 1 in the UniProtKb entry Q8IZE3. rthorne@icr.ac.uk 12-Oct-2007: Contacted by JYOTI. A human kidney Matchmaker cDNA library in the pGAD10 vector (Clontech) was used. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells confocal microscopy confocal microscopy predetermined predetermined participant predetermined Sullivan A., Uff CR., Isacke CM., Thorne RF. The sequence for PACE-1 given by the authors corresponds to the sequence variations specified by Ref. 1 in the UniProtKb entry Q8IZE3. rthorne@icr.ac.uk Accepted 2007-OCT-4 by JYOTI. 12-Oct-2007: Contacted by JYOTI. A human kidney Matchmaker cDNA library in the pGAD10 vector (Clontech) was used. Exp. Cell Res. (0014-4827) 2003 Only protein-protein interactions imex curation PACE-1, a novel protein that interacts with the C-terminal domain of ezrin. PACE-1, a novel protein that interacts with the C-terminal domain of ezrin. Exp. Cell Res. (0014-4827) 2003 imex curation Sullivan A., Uff CR., Isacke CM., Thorne RF. The sequence for PACE-1 given by the authors corresponds to the sequence variations specified by Ref. 1 in the UniProtKb entry Q8IZE3. rthorne@icr.ac.uk 12-Oct-2007: Contacted by JYOTI. A human kidney Matchmaker cDNA library in the pGAD10 vector (Clontech) was used. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Sullivan A., Uff CR., Isacke CM., Thorne RF. The sequence for PACE-1 given by the authors corresponds to the sequence variations specified by Ref. 1 in the UniProtKb entry Q8IZE3. rthorne@icr.ac.uk Accepted 2007-OCT-4 by JYOTI. 12-Oct-2007: Contacted by JYOTI. A human kidney Matchmaker cDNA library in the pGAD10 vector (Clontech) was used. Exp. Cell Res. (0014-4827) 2003 Only protein-protein interactions imex curation PACE-1, a novel protein that interacts with the C-terminal domain of ezrin. PACE-1, a novel protein that interacts with the C-terminal domain of ezrin. Exp. Cell Res. (0014-4827) 2003 imex curation Sullivan A., Uff CR., Isacke CM., Thorne RF. The sequence for PACE-1 given by the authors corresponds to the sequence variations specified by Ref. 1 in the UniProtKb entry Q8IZE3. rthorne@icr.ac.uk 12-Oct-2007: Contacted by JYOTI. A human kidney Matchmaker cDNA library in the pGAD10 vector (Clontech) was used. Only protein-protein interactions human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma confocal microscopy confocal microscopy predetermined predetermined participant predetermined Sullivan A., Uff CR., Isacke CM., Thorne RF. The sequence for PACE-1 given by the authors corresponds to the sequence variations specified by Ref. 1 in the UniProtKb entry Q8IZE3. rthorne@icr.ac.uk Accepted 2007-OCT-4 by JYOTI. 12-Oct-2007: Contacted by JYOTI. A human kidney Matchmaker cDNA library in the pGAD10 vector (Clontech) was used. Exp. Cell Res. (0014-4827) 2003 Only protein-protein interactions imex curation PACE-1, a novel protein that interacts with the C-terminal domain of ezrin. PACE-1, a novel protein that interacts with the C-terminal domain of ezrin. Exp. Cell Res. (0014-4827) 2003 imex curation Sullivan A., Uff CR., Isacke CM., Thorne RF. The sequence for PACE-1 given by the authors corresponds to the sequence variations specified by Ref. 1 in the UniProtKb entry Q8IZE3. rthorne@icr.ac.uk 12-Oct-2007: Contacted by JYOTI. A human kidney Matchmaker cDNA library in the pGAD10 vector (Clontech) was used. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Sullivan A., Uff CR., Isacke CM., Thorne RF. The sequence for PACE-1 given by the authors corresponds to the sequence variations specified by Ref. 1 in the UniProtKb entry Q8IZE3. rthorne@icr.ac.uk Accepted 2007-OCT-4 by JYOTI. A human kidney Matchmaker cDNA library in the pGAD10 vector (Clontech) was used. Exp. Cell Res. (0014-4827) 2003 Only protein-protein interactions imex curation ezri_human Ezrin EZR VIL2 p81 Cytovillin Villin-2 protein protein human Homo sapiens Human MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLHYVDNKGFPTWLKLDKKVSAQEVRKENPLQFKFRAKFYPEDVAEELIQDITQKLFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKEVHKSGYLSSERLIPQRVMDQHKLTRDQWEDRIQVWHAEHRGMLKDNAMLEYLKIAQDLEMYGINYFEIKNKKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLEEARRRKEDEVEEWQHRAKEAQDDLVKTKEELHLVMTAPPPPPPPVYEPVSYHVQESLQDEGAEPTGYSAELSSEGIRDDRNEEKRITEAEKNERVQRQLLTLSSELSQARDENKRTHNDIIHNENMRQGRDKYKTLRQIRQGNTKQRIDEFEAL DIP protein P15311 original sequence version: 170 DIP protein P15311 original sequence version: 142 F1B592CF49A7CC46 cd44_human CD44 antigen Epican CDw44 Phagocytic glycoprotein I Extracellular matrix receptor III HUTCH-I Phagocytic glycoprotein 1 GP90 lymphocyte homing/adhesion receptor Hermes antigen Hyaluronate receptor Heparan sulfate proteoglycan CD44 LHR MDU2 MDU3 MIC4 protein protein human Homo sapiens Human MDKFWWHAAWGLCLVPLSLAQIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIPATTLMSTSATATETATKRQETWDWFSWLFLPSESKNHLHTTTQMAGTSSNTISAGWEPNEENEDERDRHLSFSGSGIDDDEDFISSTISTTPRAFDHTKQNQDWTQWNPSHSNPEVLLQTTTRMTDVDRNGTTAYEGNWNPEAHPPLIHHEHHEEEETPHSTSTIQATPSSTTEETATQKEQWFGNRWHEGYRQTPKEDSHSTTGTAAASAHTSHPMQGRTTPSPEDSSWTDFFNPISHPMGRGHQAGRRMDMDSSHSITLQPTANPNTGLVEDLDRTGPLSMTTQQSNSQSFSTSHEGLEEDKDHPTTSTLTSSNRNDVTGGRRDPNHSEGSTTLLEGYTSHYPHTKESRTFIPVTSAKTGSFGVTAVTVGDSNSNVNRSLSGDQDTFHPSGGSHTTHGSESDGHSHGSQEGGANTTSGPIRTPQIPEWLIILASLLALALILAVCIAVNSRRRCGQKKKLVINSGNGAVEDRKPSGLNGEASKSQEMVHLVNKESSETPDQFMTADETRNLQNVDMKIGV DIP protein P16070 original sequence version: 142 DIP protein P16070 original sequence version: 157 BB9B66B19B970349 e2rsi6_canlf EZR protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLQYVDNKGFPTWLKLDKKVSAQEVRKENPLQFKFRAKFYPEDVSEELIQDITQKLFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKETHKSGYLSSERLIPQRVMDQHKLTRDQWEDRIQVWHAEHRGMLKDSAMLEYLKIAQDLEMYGINYFEIKNKKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAEKELSDQIQRALQLEEERKRAQEEAERLEADRVAALRAKEELERQAADQIKSQEQLAAELAEYTAKIALLEEARRRKEDEVEEWQHRAKEAQDDLVKTKEELHLVMTAPPPPPPPVYEPVSYHVQDNLQEEGTEYTGYSAELSSEGIMDDRNEEKRITEAEKNERVQRQLMTLTSELSQARDENKRTHNDIIHNENMRQGRDKYKTLRQIRQGNTKQRIDEFEAL 2267409148F8518A pace1_human Protein-associating with the carboxyl-terminal domain of ezrin SCYL3 PACE1 Ezrin-binding protein PACE-1 SCY1-like protein 3 protein protein human Homo sapiens Human MGSENSALKSYTLREPPFTLPSGLAVYPAVLQDGKFASVFVYKRENEDKVNKAAKHLKTLRHPCLLRFLSCTVEADGIHLVTERVQPLEVALETLSSAEVCAGIYDILLALIFLHDRGHLTHNNVCLSSVFVSEDGHWKLGGMETVCKVSQATPEFLRSIQSIRDPASIPPEEMSPEFTTLPECHGHARDAFSFGTLVESLLTILNEQVSADVLSSFQQTLHSTLLNPIPKCRPALCTLLSHDFFRNDFLEVVNFLKSLTLKSEEEKTEFFKFLLDRVSCLSEELIASRLVPLLLNQLVFAEPVAVKSFLPYLLGPKKDHAQGETPCLLSPALFQSRVIPVLLQLFEVHEEHVRMVLLSHIEAYVEHFTQEQLKKVILPQVLLGLRDTSDSIVAITLHSLAVLVSLLGPEVVVGGERTKIFKRTAPSFTKNTDLSLEDSPMCVVCSHHSQISPILENPFSSIFPKCFFSGSTPINSKKHIQRDYYNTLLQTGDPFSQPIKFPINGLSDVKNTSEDSENFPSSSKKSEEWPDWSEPEEPENQTVNIQIWPREPCDDVKSQCTTLDVEESSWDDCEPSSLDTKVNPGGGITATKPVTSGEQKPIPALLSLTEESMPWKSSLPQKISLVQRGDDADQIEPPKVSSQERPLKVPSELGLGEEFTIQVKKKPVKDPEMDWFADMIPEIKPSAAFLILPELRTEMVPKKDDVSPVMQFSSKFAAAEITEGEAEGWEEEGELNWEDNNW 2F7D03681A4F641B vil2-scyl3 5 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range 9 unspecified role unspecified role prey prey physical association physical association a yeast 2-hybrid screen was undertaken using ezrin as a bait. We describe here the identification and cloning of a novel protein, PACE-1 The authors show the protein Ezrin as 585 aa in length but the UniProtKb record P23714 is 586 aa in length. PREY gene AF540957 The UniProtKb record Q8IZE3 has an insertion aa 438-445 between aa 437 and 438 of the translation of the author identifier given for the prey. vil2-scyl3-1 1 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association The authors show the protein Ezrin as 585 aa in length but the UniProtKb record P23714 is 586 aa in length. PREY gene AF540957 The UniProtKb record Q8IZE3 has an insertion aa 438-445 between aa 437 and 438 of the translation of the author identifier given for the prey. a series of ezrin constructs in the pGBT9 vector were generated and tested for their interaction with pGAD10-clone 33 in the yeast 2-hybrid assay (Fig. 1B). No interaction was observed with two N-terminal ezrin constructs (1-247 and 11-247), whereas a full-length ezrin (1-585), a construct deleted in the first 10 amino acids(11-585), and a C-terminal construct (243-585) gave positive reactivity 1 B Clone 33 encodes a partial cDNA encompassing the C-terminal and 3' untranslated region of PACE-1. scyl3-ezrin 3 8 unspecified role unspecified role prey prey canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line 9 unspecified role unspecified role bait bait ecobb Escherichia coli (strain B / BL21) physical association physical association BAIT gene AF540957 The UniProtKb record Q8IZE3 has an insertion aa 438-445 between aa 437 and 438 of the translation of the author identifier given for the prey. Clone 33 encodes a partial cDNA encompassing the C-terminal and 3' untranslated region of PACE-1. Only GST clone 33 was able to interact with ezrin under stringent conditions of washing with 1% Triton X-100. 1 C cd44-scyl3 4 7 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component physical association physical association Q8IZE3 gene AF540957 The UniProtKb record Q8IZE3 has an insertion aa 438-445 between aa 437 and 438 of the translation of the author identifier given for the prey. 5 B CD44 similarly colocalised with transfected PACE-1 and PACE-1-myc proteins in the lamellar ruffles This colocalization occurred in lamellar ruffles. cd44-scyl3-1 4 6 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Q8IZE3 gene AF540957 The UniProtKb record Q8IZE3 has an insertion aa 438-445 between aa 437 and 438 of the translation of the author identifier given for PACE-1. 5 C This colocalization occurred only in the most distal part of the lamellipodia. Q8IZE3 transfection When the MDA-MB-231 cells were transfected with PACE-1-myc and costained with 9E10 anti-myc and anti-ezrin antibodies, the PACE-1 protein was found to colocalise with ezrin but only in the most distal part of the lamellipodia cd44-scyl3-2 2 6 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role neutral component neutral component physical association physical association Q8IZE3 gene AF540957 The UniProtKb record Q8IZE3 has an insertion aa 438-445 between aa 437 and 438 of the translation of the author identifier given for the prey. 5 D When MCF-7 cells were cotransfected with PACE-1 and ezrin-GFP and examined with anti-PACE-1 antibody staining and GFP fluorescence, transfected proteins were also demonstrated to colocalise P23714; Q8IZE3 transfection