UniProt UniProt http://www.uniprot.org Identification of novel protein-protein interactions at the cytosolic surface of the Sec63 complex in the yeast ER membrane. Identification of novel protein-protein interactions at the cytosolic surface of the Sec63 complex in the yeast ER membrane. Yeast 20 (133-148) 2003 Willer M., Jermy AJ., Young BP., Stirling CJ. colin.stirling@man.ac.uk 22-Jan-2008: Contacted by JYOTI. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Willer M., Jermy AJ., Young BP., Stirling CJ. colin.stirling@man.ac.uk Accepted 2007-Not a month!-11 by ORCHARD. Yeast 20 (133-148) 2003 orchard Identification of novel protein-protein interactions at the cytosolic surface of the Sec63 complex in the yeast ER membrane. Identification of novel protein-protein interactions at the cytosolic surface of the Sec63 complex in the yeast ER membrane. Yeast 20 (133-148) 2003 Willer M., Jermy AJ., Young BP., Stirling CJ. colin.stirling@man.ac.uk 22-Jan-2008: Contacted by JYOTI. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Willer M., Jermy AJ., Young BP., Stirling CJ. colin.stirling@man.ac.uk Accepted 2007-Not a month!-11 by ORCHARD. 22-Jan-2008: Contacted by JYOTI. Yeast 20 (133-148) 2003 orchard sec72_yeast Translocation protein SEC72 SEC67 SIM2 L8003.18 YLR292C SEC72 Sec62/63 complex 23 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MVTLEYNANSKLITASDAVVALSTETNIDQINVLTTSLIGETNPNFTPQPNEALSKMIKGLFESGMKNLQQKKLNEALKNVSLAIEMAQRKRAPWEAFAIQLPELHFMLRSKIDLCLILGKHLEALQDLDFLLGTGLIQPDVFVRKADCLLKLRQWEEARATCERGLALAPEDMKLRALLIETARNLAEYNGE D5D200DF2DCA81DB sec63_yeast Protein translocation protein SEC63 PTL1 YOR254C SEC63 NPL1 Sec62/63 complex 73 kDa subunit Protein NPL1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPTNYEYDEASETWPSFILTGLLMVVGPMTLLQIYQIFFGANAEDGNSGKSKEFNEEVFKNLNEEYTSDEIKQFRRKFDKNSNKKSKIWSRRNIIIIVGWILVAILLQRINSNDAIKDAATKLFDPYEILGISTSASDRDIKSAYRKLSVKFHPDKLAKGLTPDEKSVMEETYVQITKAYESLTDELVRQNYLKYGHPDGPQSTSHGIALPRFLVDGSASPLLVVCYVALLGLILPYFVSRWWARTQSYTKKGIHNVTASNFVSNLVNYKPSEIVTTDLILHWLSFAHEFKQFFPDLQPTDFEKLLQDHINRRDSGKLNNAKFRIVAKCHSLLHGLLDIACGFRNLDIALGAINTFKCIVQAVPLTPNCQILQLPNVDKEHFITKTGDIHTLGKLFTLEDAKIGEVLGIKDQAKLNETLRVASHIPNLKIIKADFLVPGENQVTPSSTPYISLKVLVRSAKQPLIPTSLIPEENLTEPQDFESQRDPFAMMSKQPLVPYSFAPFFPTKRRGSWCCLVSSQKDGKILQTPIIIEKLSYKNLNDDKDFFDKRIKMDLTKHEKFDINDWEIGTIKIPLGQPAPETVGDFFFRVIVKSTDYFTTDLDITMNMKVRDSPAVEQVEVYSEEDDEYSTDDDETESDDESDASDYTDIDTDTEAEDDESPE 7D6757144C8A301F hri1_yeast Protein HRI1 YLR301W HRI1 HRR25-interacting protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPALLKRLLFQVGPHPNERTFTLSSVSTDGHYISLRPFVKPSGDELSFPFEWAFAGTNETVKANDQGNGVVTQDFNFWLDTNVYLNVPNTHRGEVNTTWKNWDSGCVEETGAVYPFGADKESVSFRELWQPVDPSREDLVIVSPNNEKFSSNARSIVLKVTDEAYDGLVIVIGRWIQGFLSQKNNNTIEGLNFIRLLEKDSGKSEFLLSYGKEVNKIPQSYENLKKGSTVTSNGLNWEVIEYHA 80D813586A1930BB sec62_yeast Translocation protein SEC62 SEC62 YPL094C LPG14C Sec62/63 complex 30 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAVGPGSNAGASVNGGSATAIATLLRNHKELKQRQGLFQAKQTDFFRYKRFVRALHSEEYANKSARQPEIYPTIPSNKIEDQLKSREIFIQLIKAQMVIPVKKLHSQECKEHGLKPSKDFPHLIVSNKAQLEADEYFVWNYNPRTYMDYLIVIGVVSIILALVCYPLWPRSMRRGSYYVSLGAFGILAGFFAVAILRLILYVLSLIVYKDVGGFWIFPNLFEDCGVLESFKPLYGFGEKDTYSYKKKLKRMKKKQAKRESNKKKAINEKAEQN 2B2BA0DAAF3ACCA1 sec62-sec63 1 4 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association No Figure We found only two combinations that gave rise to his+ cells, viz. the N-terminal 158 residues of Sec62p fused to the Gal4p activation domain (pBY6) interacts with the C-terminal 424 residues of Sec63p fused to the Gal4p binding domain (pBY3) sec72-ylr301w 1 3 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association No figure A slightly weaker induction was found with full-length Sec72p and Ylr301wp sec72-sec72 2 3 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association No figure sec62-sec63-1 2 6 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 3 Firstly, we found that a construct (pBY20) expressing the last 51 residues of Sec63p (residues 612-663 fused to the Gal4p activation domain) interacted strongly with the N-terminal Sec62 fusion (Figure 3A).