UniProt UniProt http://www.uniprot.org NOSTRIN: a protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase. NOSTRIN: a protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 imex curation Zimmermann K., Opitz N., Dedio J., Renne C., Muller-Esterl W., Oess S. wme@biochem2.de 12-Oct-2007: Contacted by JYOTI. human placenta cDNA library Only protein-protein interactions imex curation in vitro In vitro pull down pull down predetermined predetermined participant predetermined Zimmermann K., Opitz N., Dedio J., Renne C., Muller-Esterl W., Oess S. wme@biochem2.de wme@biochem2.de. Accepted 2007-OCT-25 by JYOTI. 12-Oct-2007: Contacted by JYOTI. human placenta cDNA library Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 IMEx NOSTRIN: a protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase. NOSTRIN: a protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 imex curation Zimmermann K., Opitz N., Dedio J., Renne C., Muller-Esterl W., Oess S. wme@biochem2.de 12-Oct-2007: Contacted by JYOTI. human placenta cDNA library Only protein-protein interactions imex curation crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. confocal microscopy confocal microscopy anti tag immunost anti tag immunostaining anti tag immunost Zimmermann K., Opitz N., Dedio J., Renne C., Muller-Esterl W., Oess S. wme@biochem2.de wme@biochem2.de. Accepted 2007-OCT-25 by JYOTI. 12-Oct-2007: Contacted by JYOTI. human placenta cDNA library Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 IMEx NOSTRIN: a protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase. NOSTRIN: a protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 imex curation Zimmermann K., Opitz N., Dedio J., Renne C., Muller-Esterl W., Oess S. wme@biochem2.de 12-Oct-2007: Contacted by JYOTI. human placenta cDNA library Only protein-protein interactions imex curation crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti bait coip anti bait coimmunoprecipitation anti bait coip antibody detection identification by antibody antibody detection Zimmermann K., Opitz N., Dedio J., Renne C., Muller-Esterl W., Oess S. wme@biochem2.de wme@biochem2.de. Accepted 2007-OCT-25 by JYOTI. 12-Oct-2007: Contacted by JYOTI. human placenta cDNA library Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 IMEx NOSTRIN: a protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase. NOSTRIN: a protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 imex curation Zimmermann K., Opitz N., Dedio J., Renne C., Muller-Esterl W., Oess S. wme@biochem2.de 12-Oct-2007: Contacted by JYOTI. human placenta cDNA library Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Zimmermann K., Opitz N., Dedio J., Renne C., Muller-Esterl W., Oess S. wme@biochem2.de wme@biochem2.de. Accepted 2007-OCT-25 by JYOTI. 12-Oct-2007: Contacted by JYOTI. human placenta cDNA library Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 IMEx NOSTRIN: a protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase. NOSTRIN: a protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 imex curation Zimmermann K., Opitz N., Dedio J., Renne C., Muller-Esterl W., Oess S. wme@biochem2.de 12-Oct-2007: Contacted by JYOTI. human placenta cDNA library Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Zimmermann K., Opitz N., Dedio J., Renne C., Muller-Esterl W., Oess S. wme@biochem2.de wme@biochem2.de. Accepted 2007-OCT-25 by JYOTI. human placenta cDNA library Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 IMEx nostn_human Nostrin eNOS-trafficking inducer Nitric oxide synthase trafficker Nitric oxide synthase traffic inducer BM247 homolog NOSTRIN protein protein human Homo sapiens Human MRDPLTDCPYNKVYKNLKEFSQNGENFCKQVTSVLQQRANLEISYAKGLQKLASKLSKALQNTRKSCVSSAWAWASEGMKSTADLHQKLGKAIELEAIKPTYQVLNVQEKKRKSLDNEVEKTANLVISNWNQQIKAKKKLMVSTKKHEALFQLVESSKQSMTEKEKRKLLNKLTKSTEKLEKEDENYYQKNMAGYSTRLKWENTLENCYQSILELEKERIQLLCNNLNQYSQHISLFGQTLTTCHTQIHCAISKIDIEKDIQAVMEETAILSTENKSEFLLTDYFEEDPNSAMDKERRKSLLKPKLLRLQRDIEKASKDKEGLERMLKTYSSTSSFSDAKSQKDTAALMDENNLKLDLLEANSYKLSSMLAELEQRPQPSHPCSNSIFRWREKEHTHSYVKISRPFLMKRLENIVSKASSGGQSNPGSSTPAPGAAQLSSRLCKALYSFQARQDDELNLEKGDIVIIHEKKEGGWWFGSLNGKKGHFPAAYVEELPSNAGNTATKA DC9A6DF5B9C2AED0 nos3_human Nitric oxide synthase, endothelial Endothelial NOS EC-NOS NOS type III Constitutive NOS NOS3 protein protein human Homo sapiens Human MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSAQAEGLQLLPGLIHVHRRKMFQATIRSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPAPTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTFFLDITSPPSPQLLRLLSTLAEEPREQQELEALSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSTHPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKPGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKGLQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATNVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDPPGSDTNSP DIP protein P29474 original sequence version: 179 DIP protein P29474 original sequence version: 175 DIP protein P29474 original sequence version: 112 DIP protein P29474 original sequence version: 140 DIP protein P29474 original sequence version: 158 B761A6D40B1A5649 nos3-nostrin-1 5 6 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 7 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association nos3-nostrin-2 3 7 unspecified role unspecified role bait bait crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 6 unspecified role unspecified role prey prey crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association 4A nostrin-nos3-2 3 7 unspecified role unspecified role prey prey crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 6 unspecified role unspecified role bait bait crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association 4A nos3-nostrin-3 4 7 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 6 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association 4b nostrin-nos3-1 4 7 unspecified role unspecified role prey prey region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 6 unspecified role unspecified role bait bait sh3 domain binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association 4b nostrin-nos3-3 1 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh3 domain binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 4C nostrin-nos3-4 2 7 unspecified role unspecified role neutral component neutral component crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 6 unspecified role unspecified role neutral component neutral component crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. colocalization colocalization 5