IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Protein-protein interactions between sucrose transporters of different affinities colocalized in the same enucleate sieve element. Protein-protein interactions between sucrose transporters of different affinities colocalized in the same enucleate sieve element. Plant Cell (1040-4651) 2002 imex curation Reinders A., Schulze W., Kuhn C., Barker L., Schulz A., Ward JM., Frommer WB. To reduce signals caused by autoactivation or colocalization in the same compartment, an improved system was developed using the weak wheat germ agglutinin binding protein ( WBP1 ) promoter driving the Cub fusion. frommer@zmbp.uni-tuebingen.de; 22-Jan-2008: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast ub reconstruction ubiquitin reconstruction ub reconstruction predetermined predetermined participant predetermined Reinders A., Schulze W., Kuhn C., Barker L., Schulz A., Ward JM., Frommer WB. To reduce signals caused by autoactivation or colocalization in the same compartment, an improved system was developed using the weak wheat germ agglutinin binding protein ( WBP1 ) promoter driving the Cub fusion. frommer@zmbp.uni-tuebingen.de; Accepted 2007-DEC-14 by JYOTI. Plant Cell (1040-4651) 2002 Only protein-protein interactions imex curation q40167_sollc LeSUT1 protein protein sollc Solanum lycopersicum (Tomato) Lycopersicon esculentum Tomato MENGTKGKLTVSSSLQIEQPLPPSKLWKIIVVASIAAGVQFGWALQLSLLTPYVQLLGIPHRFASFIWLCGPISGMIVQPVVGYYSDNCSSRFGRRRPFIAAGAALVTIAVFLIGFAADLGHASGDPLGKGSKPRAIAVFVVGFWILDVANNMLQGPCRALLADLSGGKSGKMRTANAFFSFFKAVGNILGYAAGSYSRLFKVFPFSKTKACDMYRANLKSCFFIAIFLLLSLTTLALTLVRENELPEKEELEIDEKLSGAGKSKVPFFGEIFGALKDLPRPMWILLLVTCLNWIAWFPFFLYDTDWMAKEVFGGQVGDAKLYDLGVRAGALGLLLQSVVLGFMSLGVEFLGKKIGGAKRLWGILNFVLAICLAMTILVTKMAEKSRGHDAAGTLMGPTPGVKIGALLLFAALGIPLAVTFSIPFALAS 62024DC53BC72B1C q9fvj6_sollc SUT4 protein protein sollc Solanum lycopersicum (Tomato) Lycopersicon esculentum Tomato MPEIERHRTRHNRPAIREPVKPRVPLRLLLRVASVAGGIQFGWALQLSLLTPYVQELGIPHAWASIIWLCGPLSGLLVQPLVGHMSDKCTSRFGRRRPFIVAGAASIMIAVLIIGFSADIGWLLGDRGEIKVRAIAAFVVGFWLLDVANNMTQGPCRALLADLTQKDHRRTRVANAYFSLFMAIGNILGFATGSYSGWYKIFLFTLNTACTINCANLKAAFILDIIFIATTTCISISAANEQPLDPSRGSSHTGEEIDESSHGQEEAFLWELFGIFKYFPGVVWVILLVTALTWIGWFPFLLFDTDWFGREIYGGEPNDGKNYSAGVRMGSLGLMLNSVLLGLTSLFMEKLCRKWGAGFTWGVSNVVMSLCFIAMLIITAVRSNIDIGQGLPPDGIVIAALVVFSILGIPLAITYSVPYALVSSRIEALGLGQGLSMGVLNLAIVFPQIVVSLGSGPWDELFGGGNSPAFVVAALSAFAAGLIAILAIPRTRVERPKIFA BFDFAE7468D40110 q9fvl6_sollc SUT2 protein protein sollc Solanum lycopersicum (Tomato) Lycopersicon esculentum Tomato MDAVSIRVPYKNLKQQEVELTNVDESRFAQLEIRSDSSSPRVSNGEMNDSNLPLPPPPVRNSLLTLILSCTVAAGVQFGWALQLSLLTPYIQTLGIEHAFSSFIWLCGPITGLVVQPCVGIWSDKCHSKYGRRRPFIFIGAVMISIAVIIIGFSADIGYLLGDTKEHCSTFKGTRSRAAIVFVVGFWMLDLANNTVQGPARALLADLSGPDQRNTANAVFCSWMAVGNILGFSAGASGGWHRWFPFLTNRACCEPCGNLKAAFLVAVVFLTLCTLVTLYFANEVPLSPKQYKRMSDSAPLLDSPQNTGFDLSQSKRELQYRNSVANNESEMGHVADNSPKNEEQRPDKDQGDSFADSPGAVLVNLLTSLRHLPPAMHSVLIVMALTWLSWFPFFLFDTDWMGREVYHGDPKGEADEVNAYNQGVREGAFGLLLNSVVLGVSSFLIEPMCKWIGSRLVWAVSNFIVFVCMACTAIISVVSISANTQGVQHVIGATRSTQIAALVVFSLLGIPLAVTYSVPFSITAELTADAGGGQGLAIGVLNLAIVVPQMVVSLGAGPWDALFGGGNIPAFALASLAALAAGIFAMLRLPNLSSNFKSTGFHFG 3403349B1692B2AF sut1-2 1 2 unspecified role unspecified role prey prey c-terminal binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1552185]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 510-510, sequence length 429 [EBI-1552185]210-510 n-terminal NubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 2 unspecified role unspecified role bait bait n-terminal binding region binding-associated region binding region certain certain sequence position certain range range c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 4 To test this, LeSUT1-CubPLV and LeSUT2-CubPLV were used as bait and NubG fusions of LeSUT1, LeSUT2, and LeSUT4 were used as prey (Figure 3). Yeast cells expressing LeSUT1 or LeSUT2 in combination with either of the two other Suc transporters as prey, growth on His-free medium (data not shown), and -galactosidase filter assays demonstrated the potential for both Suc transporters to interact with their paralogs, indicating the potential of Suc transporters to form heterooligomeric complexes (Figure 4). The amino acid coordinates of the N and C terminal regions of the protein used in the study are not provided. Therefore the protein is represented as two halfs with fuzzy coordinates used to represent the approximated coordinates. sut1-sut2 1 2 unspecified role unspecified role bait bait n-terminal binding region binding-associated region binding region certain certain sequence position certain range range c-terminal NubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role prey prey c-terminal binding region binding-associated region binding region range range certain certain sequence position certain n-terminal NubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 4 To test this, LeSUT1-CubPLV and LeSUT2-CubPLV were used as bait and NubG fusions of LeSUT1, LeSUT2, and LeSUT4 were used as prey (Figure 3). Yeast cells expressing LeSUT1 or LeSUT2 in combination with either of the two other Suc transporters as prey, growth on His-free medium (data not shown), and -galactosidase filter assays demonstrated the potential for both Suc transporters to interact with their paralogs, indicating the potential of Suc transporters to form heterooligomeric complexes (Figure 4). The amino acid coordinates of the N and C terminal regions of the protein(s) used in the study are not provided. Therefore the protein is represented as two halfs with fuzzy coordinates used to represent the approximated coordinates. sut1-sut4 1 3 unspecified role unspecified role prey prey c-terminal binding region binding-associated region binding region range range certain certain sequence position certain n-terminal NubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 2 unspecified role unspecified role bait bait n-terminal binding region binding-associated region binding region certain certain sequence position certain range range c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 4 To test this, LeSUT1-CubPLV and LeSUT2-CubPLV were used as bait and NubG fusions of LeSUT1, LeSUT2, and LeSUT4 were used as prey (Figure 3). Yeast cells expressing LeSUT1 or LeSUT2 in combination with either of the two other Suc transporters as prey, growth on His-free medium (data not shown), and -galactosidase filter assays demonstrated the potential for both Suc transporters to interact with their paralogs, indicating the potential of Suc transporters to form heterooligomeric complexes (Figure 4). The amino acid coordinates of the N and C terminal regions of the protein(s) used in the study are not provided. Therefore the protein is represented as two halfs with fuzzy coordinates used to represent the approximated coordinates. sut2 1 4 unspecified role unspecified role prey prey c-terminal binding region binding-associated region binding region range range certain certain sequence position certain n-terminal NubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role bait bait n-terminal binding region binding-associated region binding region certain certain sequence position certain range range c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 4 To test this, LeSUT1-CubPLV and LeSUT2-CubPLV were used as bait and NubG fusions of LeSUT1, LeSUT2, and LeSUT4 were used as prey (Figure 3). Yeast cells expressing LeSUT1 or LeSUT2 in combination with either of the two other Suc transporters as prey, growth on His-free medium (data not shown), and -galactosidase filter assays demonstrated the potential for both Suc transporters to interact with their paralogs, indicating the potential of Suc transporters to form heterooligomeric complexes (Figure 4). The amino acid coordinates of the N and C terminal regions of the protein(s) used in the study are not provided. Therefore the protein is represented as two halfs with fuzzy coordinates used to represent the approximated coordinates. sut2-sut4 1 4 unspecified role unspecified role bait bait n-terminal binding region binding-associated region binding region certain certain sequence position certain range range c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 3 unspecified role unspecified role prey prey c-terminal binding region binding-associated region binding region range range certain certain sequence position certain n-terminal NubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 4 To test this, LeSUT1-CubPLV and LeSUT2-CubPLV were used as bait and NubG fusions of LeSUT1, LeSUT2, and LeSUT4 were used as prey (Figure 3). Yeast cells expressing LeSUT1 or LeSUT2 in combination with either of the two other Suc transporters as prey, growth on His-free medium (data not shown), and -galactosidase filter assays demonstrated the potential for both Suc transporters to interact with their paralogs, indicating the potential of Suc transporters to form heterooligomeric complexes (Figure 4). The amino acid coordinates of the N and C terminal regions of the protein(s) used in the study are not provided. Therefore the protein is represented as two halfs with fuzzy coordinates used to represent the approximated coordinates. sut2-sut1 1 2 unspecified role unspecified role prey prey c-terminal binding region binding-associated region binding region range range certain certain sequence position certain n-terminal NubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role bait bait n-terminal binding region binding-associated region binding region certain certain sequence position certain range range c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 4 To test this, LeSUT1-CubPLV and LeSUT2-CubPLV were used as bait and NubG fusions of LeSUT1, LeSUT2, and LeSUT4 were used as prey (Figure 3). Yeast cells expressing LeSUT1 or LeSUT2 in combination with either of the two other Suc transporters as prey, growth on His-free medium (data not shown), and -galactosidase filter assays demonstrated the potential for both Suc transporters to interact with their paralogs, indicating the potential of Suc transporters to form heterooligomeric complexes (Figure 4). The amino acid coordinates of the N and C terminal regions of the protein(s) used in the study are not provided. Therefore the protein is represented as two halfs with fuzzy coordinates used to represent the approximated coordinates.