UniProt UniProt http://www.uniprot.org Ady3p links spindle pole body function to spore wall synthesis in Saccharomyces cerevisiae. Ady3p links spindle pole body function to spore wall synthesis in Saccharomyces cerevisiae. Genetics 160 (1439-1450) 2002 imex curation Nickas ME., Neiman AM. aaron.neiman@sunysb.edu 22-Jan-2008: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Nickas ME., Neiman AM. aaron.neiman@sunysb.edu Accepted 2007-Not a month!-20 by CDEROW. 22-Jan-2008: Contacted by JYOTI. Genetics 160 (1439-1450) 2002 Only protein-protein interactions imex curation Ady3p links spindle pole body function to spore wall synthesis in Saccharomyces cerevisiae. Ady3p links spindle pole body function to spore wall synthesis in Saccharomyces cerevisiae. Genetics 160 (1439-1450) 2002 imex curation Nickas ME., Neiman AM. aaron.neiman@sunysb.edu 22-Jan-2008: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Nickas ME., Neiman AM. aaron.neiman@sunysb.edu Accepted 2007-Not a month!-20 by CDEROW. Genetics 160 (1439-1450) 2002 Only protein-protein interactions imex curation nud1_yeast Protein NUD1 YOR373W NUD1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDMDTQEAELSSQLENLTINSPRKLRSNAHSNSGKVFKEYESNHDFQDSNFTSQVVEPAISDSVKKPPTMTVLNNYSTVHQKVPSGFSGTTATSHQEAQWKQYFPGIGSGGGTNFGGAVGTANKVPESDLIVSDLVKDLSGVLETNTFKRHLDMKNKTTTMQTHENHDTISISHSKDFFNAEKVSSSFSDDSDSGPAAEAHDVFDGILQKQKSNYLVGSYPSNSNNKNNNNNNNNNNNNSININNKDNARTKEEDEEDTSNSFEFSSSSSMSSSQTQSGRKSKVLKKPPLNTISPGQLGYQFNHTHGAWDPPLNQGLDVSSSHSLDNTSSNQSQFATMVPTGDNHTNGKAPSILDKKAYELTSTKPGDVGYRQKKIQEEENLANSDDTPLDTPKFNDLFTKNGTRAKVKGQMRTSRSISNSNLLEAHKKLKTFPAERVEDITSISEVNTSFNETEKQLISILTSKLSGSPSYDSDWEKILKVDLSRGKLKNMFGMQRLLPNVLVLNLSDNEMNTLEGIPSNVVQLFCSNNKITSAHCSLAGFHDLECLDLSYNLLNTSLKFLSLCHHLQEVNLSYNSIQSLEGIGSSRMKKLNLSNNEINGIIDFEQLILTNNSVVGGWLTVEVLDLSNNNIIGVRNINCLPRLKVLNLNGNPLVSIVESSKMENGTLRALSIKNTGGALSKLQNYKLDDQFTFPYQNLKILKLDGFAQLSKWQKWPATLQILEINGGLASSLPRFSSLKSTNLYSLTIANVRDFTHLPVDLSKELPFLQELHLPGNNLQNAHKLTKTLPRQSVKFLDLRNNPITTPRHDRASTSLHYRQLLQLAGLCQQQCPALATLWLDDTPAPTATNL CB9F0408633C1315 gip1_yeast GLC7-interacting protein 1 YBR0420 YBR045C GIP1 protein protein yeast Saccharomyces cerevisiae Baker's yeast METILQPKARPFESLKRKRFREWLRPSTAHGSLLHSDTLDLRDFAKPNPADTFSNLDSGHCPLVTTPIKYECPDGKSSFFRGDTKFETLFSNRKFYEFKDNLKRGLKKIRHGRNGHQSEKRCPVVEETKKSVSDNLDKPDNNTPCFDRFHTNSKEFETQFDHSNRSQNSEKAYLDNESCWNLSEKFIPFNNLKYEDLKHFEENLQSLAPATFTPIESNESLDRSDSTRGTKRSIRNDSSDTTSEKRLCLKQYSDEPESDHSMESTPSIYITKEVQERIEALSSTDSFLIEKVDFPSNKIGSSASDYESDNEYRNMDEDSINDVTTEKEGNVVIPDSNTSTVDAMEKPIEVSSALKDDTLDKDIDDASSSYSDDVETTFEPVESEELSDLSDTSSSGSSKIYTIPTFRGLTNRTNISQILSKVGKADLSQDNLTHLIKSHQKKKRCVNFRNKRFYDAFNPYVDNEEDAELSDSENISEMDTDLCIKDRSTSSVRFDENSRLLIYKKSKKLNKDETQSGYSTTEMRSILKTKMNSQHDEESQRASKCDTVGVAQFLHYFQYTEYKRQRNEAENYRLRGEQLSKYYSEEYPLDFAAVECEDSVNDKSDIILSMRATERNIGRQLKGISSQGAQIISLDEDVF 7B7689B82F7965C6 7B7689B82F7965C6 cnm67_yeast Chaotic nuclear migration protein 67 CNM67 N1264 YNL225C protein protein yeast Saccharomyces cerevisiae Baker's yeast MTDFDLMNFPFHERLDSPVSENGEIKDGEPIPQNWLNENHVGKSILPLFVNPEDVINCNFSNARDSYEENKSPSMDQMNYARNTSYQESPGLQERPKNEKDKSPIGTDVHKKDVPNFIHSTPRENSSKHFTRANEQASAQPTDEHTSPDISIEDCNGAKIFLQNSLSKEDFRMLENVILGYQKKVIELGRDNLRQEERANSLQKELEAATKSNDKTLDNKKKIEEQTVLIENLTKDLSLNKEMLEKANDTIQTKHTALLSLTDSLRKAELFEIPIGILFFDLYDSEENSSKLDHILQEKYPNIKGFLCASQQEELSRISQRFKNAKAEAEDLRNELENKKIEIQTMREKNNTLIGTNKTLSKQNKILCDKFDKLTIDEKEILKGCNEEIKIKLERLNERLGSWEKSKEKYETSLKDKEKMLADAEKKTNTLSKELDNLRSRFGNLEGNTSERITIKNILQSRPDISAEECNFLMVEQIDSANLTTLQNTVKEIVLAVGIPYPKLRRKIPLLAIKLKYENIMLSNFAQRLHRQVYSQEMNLKKFTDQAYYDFMSTRRMDSIDHHLERCLDHLYDHILEKMVK C4DE7631A70E695A C4DE7631A70E695A spc42_yeast Spindle pole body component SPC42 SPC42 YKL255 YKL042W protein protein yeast Saccharomyces cerevisiae Baker's yeast MNGSPTPKRYSSKSSRLYDDYYNIPYQYSNPTPMNRDYNDVGSRINADKLVPEEYKRNTEFINKAVQQNKELNFKLREKQNEIFELKKIAETLRSKLEKYVDITKKLEDQNLNLQIKISDLEKKLSDANSTFKEMRFPKVKDPMVDDDPVSENYDQINVPKHRAPDATGNPRTTNKVSNTSDQDSRLKAIERTLSVLTNYVMRSEDGNNDRMSPLPSPLNTILPINNRLNFQEPKRYNPTVKVNPSDDDIMMYESAELKRVEEEIEELKRKILVRKKHDLRKLSLNNQLQELQSMMDGDDNIKLDNVSKHNHATHRHSSQSSRDYSPSSDACLECSNDLYEKNRVKPENNMSETFATPTPNNR E3B56573FF516CE2 pp12_yeast Serine/threonine-protein phosphatase PP1-2 GLC7 CID1 DIS2 YER133W protein protein yeast Saccharomyces cerevisiae Baker's yeast MDSQPVDVDNIIDRLLEVRGSKPGQQVDLEENEIRYLCSKARSIFIKQPILLELEAPIKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFILRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIIDEKIFCMHGGLSPDLNSMEQIRRVMRPTDIPDVGLLCDLLWSDPDKDIVGWSENDRGVSFTFGPDVVNRFLQKQDMELICRAHQVVEDGYEFFSKRQLVTLFSAPNYCGEFDNAGAMMSVDESLLCSFQILKPAQKSLPRQAGGRKKK 71B20E3A4ED93235 71B20E3A4ED93235 ady3_yeast Accumulates dyads protein 3 YDL239C ADY3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNHWLAFLNKPESLKEQNSDCDQQGEMRHVTDGTLTKSPESKPFRERRSQTWIDSEVPTSTEKSNVQESISSDIISKLSNRRSRRNRSESWAGSEASSPSGNISTLENATEKNTLKSPNKFLQRGGLPTVGIGSQALSPAGKPSTLGNVSPGKFTTYKVHNSIEVNRFSSTPTKLLTNPHKVAAISNDEHYVVSNESLEENIEVAHLENVFRSSKTPDEEQSEYMKLGEIRLSSSSYGGSISKENSLPKVLDELQSQNEEIKALRQKLEEKDDRIQELEELNSMNDAKLQRIEDLQKEFHNERKAASKRLNIVQDRFRKEIKKIREEKITDFQNKNASKKEKNEVTSAKTKCKAFSQRNILVSELYRKQKQILNLQQENDKFLKDINESNNSIVKLRSEVEILKSNLQLSQDENKKLHDNGSFYEKRLNDVYSYMQNLSLFEKDLGKFILEEMKCGHSPSMFQNGFAKLYPDFQDIKNLENMEQYKQLKGKIELLEKNDRIRLEKIISVFKLINERLHFMQQQHSHKIKYLQKEALTKEQQFRLEKRRWHDILNLKEENFQKLKSELKEKLILSEKIQKNAEDKLNDYMNEHQEIVEKLQNQALIASRWSTQIQESENTHKKITDELAGKQSEILKLEETILSLKEDVFQEKLNLKKLYGDPSTELNFETVGKSFPHITKEKYDSLGLDILTDLTYVQSQNLIKNLLIVLDIPLKTFLKIVPTIVIQLRCELTLLTKFANDLNLKVFGKQLDFKSRRKVAMNEFLNNHDIAEVKHPLEYDLQALFKYFFS 46F17474F30FD3D9 46F17474F30FD3D9 mpc70_yeast Sporulation-specific protein 21 Meiotic plaque component protein 70 SPO21 MPC70 YOL091W protein protein yeast Saccharomyces cerevisiae Baker's yeast MDNILKASNMEGTSTMTVTSRSSEDSSCISNHEQDTDTHKDGDTSGLENSKISKRKWMKEFFKLSKSPASKSSRSIGSMKSNQSLVSMKSSDDGNSYKNDYSSICGNSLPSAGLSRSNSVKELKLDSTGSQRSKNNVAMLARSSTTSQTTCSSSSSSSSYNSIKGNENDILLQNNNHFRHNKEIPQSKGSSNINTASIMSQYNVDTQATAIMSDMQKQYDSQQMTSPFVNEDLHFDPNGEVSHVIKAIFKEIGYKYDDFSDIPVFQLMQEMYQLVKKNSSARRTKITDYASKLKEKEAQLKSQNDKILKLETTNKAYKTKYKEVSLENKKIKEAFKELDNESYNHDEELLKKYKYTRETLDRVNREQQLIIDQNEFLKKSVNELQNEVNATNFKFSLFKEKYAKLADSITELNTSTKKREALGENLTFECNELKEICLKYKKNIENISNTNKNLQNSFKNERKKVLDLRNERNLLKKEILLIECHGSYSLLLVSNILTCYRFLLPSDTIIETESLIKELLNMNNSLSNHVSSSDEPPAEYSKRLELKCVEFEEKLLYFYQELVTKKIIDVIYKCFINYYKKSRQTDQKSNQNSSTPYKQSQRQVPHSIK E216B0C9B865B412 E216B0C9B865B412 don1_yeast Donuts protein 1 DON1 D9954.4 YDR273W protein protein yeast Saccharomyces cerevisiae Baker's yeast MGKKNRKGKENNAAKTSFLKVENIKNTNSGLELPSQDYTNVEEKESSPKTDFPLITKEHVNTKTDSNILDYPTIGDLVSSVEKLCVLKELKIAFPEVDDTLIKAILIASQGVLEPAFNSLLYYSSPEENTDFALPMKPISVEDYSKINVSEILQREIFDDIEDEFSGQGINGSMVISKIESELSSLAEHIGNISTPGSNREVAESTRNVAVAEGHNTILSNEDSILKGKEKGKEEEKEKGEEKGVNSLKGAAVKVVAKSLKNNRIPVTVKRNEPSNNLFDVLNCDESEEEEEQDVETNTSNQERKNQGGNTEVPEAQRDSADRLPAKDDGGYKSAFGTDSCGLFAADAKDEKKQVHPSRQELSFT 6E13542050862709 6E13542050862709 ady3-cnm67 2 8 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association Table 4 In addition to Mpc70p, Ady3p interacted specifically with fusions con-taining sequences from Cnm67p and Nud1p, compo-nents of the outer plaque during both mitotic and meiotic growth, and the central plaque protein Spc42p ady3-mpc70 2 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table 4 In addition to Mpc70p, Ady3p interacted specifically with fusions con-taining sequences from Cnm67p and Nud1p, compo-nents of the outer plaque during both mitotic and meiotic growth, and the central plaque protein Spc42p ady3-nud1 2 3 unspecified role unspecified role prey prey region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1566973]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 852-852, sequence length 851 [EBI-1566973]405-852 8 unspecified role unspecified role bait bait physical association physical association Table 4 In addition to Mpc70p, Ady3p interacted specifically with fusions con-taining sequences from Cnm67p and Nud1p, compo-nents of the outer plaque during both mitotic and meiotic growth, and the central plaque protein Spc42p ady3-spc42 2 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Table 4 In addition to Mpc70p, Ady3p interacted specifically with fusions con-taining sequences from Cnm67p and Nud1p, compo-nents of the outer plaque during both mitotic and meiotic growth, and the central plaque protein Spc42p glc7-gip1-1 2 7 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association Table 4 nud1-nud1 2 3 unspecified role unspecified role prey prey region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1567005]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 852-852, sequence length 851 [EBI-1567005]405-852 3 unspecified role unspecified role bait bait region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1567002]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 852-852, sequence length 851 [EBI-1567002]405-852 physical association physical association Table 4 nud1-mpc70 2 3 unspecified role unspecified role bait bait region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1567018]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 852-852, sequence length 851 [EBI-1567018]405-852 9 unspecified role unspecified role prey prey physical association physical association Table 4 cnm67-spc42 2 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association Table 4 ady3-don1-1 1 10 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role neutral component neutral component region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 7 Both Ady3p-CFP and Don1p-YFP formed rings around the spindles during meiosis II, and these structures were coincident throughout the second mei-otic division Both Ady3p-CFP and Don1p-YFP formed rings around the spindles during meiosis II, and these structures were coincident throughout the second meiotic division.