UniProt UniProt http://www.uniprot.org The GEX-2 and GEX-3 proteins are required for tissue morphogenesis and cell migrations in C. elegans. The GEX-2 and GEX-3 proteins are required for tissue morphogenesis and cell migrations in C. elegans. Genes Dev. (0890-9369) 2002 imex curation Soto MC., Qadota H., Kasuya K., Inoue M., Tsuboi D., Mello CC., Kaibuchi K. craig.mello@umassmed.edu 01-Feb-2008: Contacted by JYOTI. orchard Only protein-protein interactions caeel Caenorhabditis elegans anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Soto MC., Qadota H., Kasuya K., Inoue M., Tsuboi D., Mello CC., Kaibuchi K. craig.mello@umassmed.edu Accepted 2008-FEB-8 by ORCHARD. 01-Feb-2008: Contacted by JYOTI. Genes Dev. (0890-9369) 2002 orchard Only protein-protein interactions imex curation The GEX-2 and GEX-3 proteins are required for tissue morphogenesis and cell migrations in C. elegans. The GEX-2 and GEX-3 proteins are required for tissue morphogenesis and cell migrations in C. elegans. Genes Dev. (0890-9369) 2002 imex curation Soto MC., Qadota H., Kasuya K., Inoue M., Tsuboi D., Mello CC., Kaibuchi K. craig.mello@umassmed.edu 01-Feb-2008: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Soto MC., Qadota H., Kasuya K., Inoue M., Tsuboi D., Mello CC., Kaibuchi K. craig.mello@umassmed.edu Accepted 2008-FEB-8 by ORCHARD. 01-Feb-2008: Contacted by JYOTI. Genes Dev. (0890-9369) 2002 orchard Only protein-protein interactions imex curation The GEX-2 and GEX-3 proteins are required for tissue morphogenesis and cell migrations in C. elegans. The GEX-2 and GEX-3 proteins are required for tissue morphogenesis and cell migrations in C. elegans. Genes Dev. (0890-9369) 2002 imex curation Soto MC., Qadota H., Kasuya K., Inoue M., Tsuboi D., Mello CC., Kaibuchi K. craig.mello@umassmed.edu 01-Feb-2008: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Soto MC., Qadota H., Kasuya K., Inoue M., Tsuboi D., Mello CC., Kaibuchi K. craig.mello@umassmed.edu Accepted 2008-FEB-8 by ORCHARD. 01-Feb-2008: Contacted by JYOTI. Genes Dev. (0890-9369) 2002 orchard Only protein-protein interactions imex curation gex3_caeel Membrane-associated protein gex-3 Gut on exterior protein 3 gex-3 F28D1.10 protein protein caeel Caenorhabditis elegans MAYKDARQVKIAEKLVILNDRAAGMMTRIYNIKKSSGDSKVKPQFLSDKKMEGAIKHIVRKFPVVDCRSNSSTFEYVQEKSTEITKSLSLYYYTFADILDLKDHIMQVLTTMESCQCQLDVTLNYDLTTSYLNLVVHLVTMMILLSRIEDRKAVLGLFNAAYDLQHGQSEASFPRLGQMILDYENPLKKLHEDLGPLNRLIYSALSSVTHTYQRRNKTADSWRTSNVFSLTAAPAQILYAAQTETIACEYLSLDVIDRWIVFCGTVCHSTLLNDDNIRHMWQLALQMNLCLRVFRDETFIVHQEIQAFLESSKEKSKRLQDVKDAFNHASVTAVAVHADRRRFLRSSLRELSLLLRDQPGLLGPKILYVWMALGAGRDEVIWLLRHQVEVPAISKKGSRMVEELVDRQLPELLFYMLELRDLVTKYYAVIQRYYLQYVSSYDSIVVSEEINQANGLTQEEAVLLTDFANEIGNINSDTDLRALRLDWFRFQAWTSAARSHFQLSRHKKLAIYMNTSVFHLKMIDLQDEMLRETSDLSLYCFYPKLAEKHWLNCLQLPAQARYVLSFARLAAHFTSALHDMCPEEKAFITEKALAQCNSVIEETCKQLSYILEKVAEHEFGLAYQMTPSAVAVRVVAQVVQQKGSGKAAAAAAAAARDYFIAGEESYRVDRQALTMPDKLQTTLLEISAALGAHRQIYVADHTFAPRTYLAQSLETKFVELLHGMFWEGQPHASNPKRPSEMLLALQAYMTVLQNLDTAISVDISNTMQTTLLQQTQLVDSKNKDTIAALYTKWYLEVLLRRASSGHMVWSEHLRTMLSSGQEQLSFMPDHYSDPQELRALVQIIGPYGVKLMTERLIWHVASQIMEMSKIVATYKDALQIARSNFDNAEKMKDVLNLLSVEPKDKKTPNATCAADAILQRTIIIGQICLFRDALHDALRHIVESKLPFLQASFDMLYHNLDDVDKVKIGEMSAAMGVTGPVDMSLVNAVRAQNPNIHPQEHYVNSCLLMVAVAICIPRIGMSDLSSYKPSIQASLNNSHCVPMAINTIGSALFHLHEQNDIQSRMKEFLALASSGILRTIHERDNSRQISDDVLRSHTTLYIILEQMVRKNRWLSMDVLETCFPYNLVRTAYQQCYEADAQ FFCD8A74A5AED610 cyfip_caeel Cytoplasmic FMR1-interacting protein homolog Gut on exterior protein 2 gex-2 F56A11.1 protein protein caeel Caenorhabditis elegans MNANVTVDDAISNVNLLDTLAIPDDLPDIEARALPLLYRSNFDTNFEDRSAFVTGIAKYSEEATRHAQFNDMLSEGLQHAANMYTWRCCSRAVPMAKSNDQPNRTEINEMVVEVLKPEVSKLGSFMRFTLTAIQRFCEEVRRLCHSEKRRDFVSEAYLLTLGRFINMFAVLDELKNMKASIKNDFSTFRRASQFLTAMSDTQAVHDMQNLSMFLATQNKIKDDLKLQMKTIEGYEELLCDVVNICAHMYEHQLYLSPNEKHMFVKVIAFSLFLMDGDAANVAKLDQKKRLSISRLDKIFKTLEVVPLYGDMQIQPFAFVRRSSHYEPSKWPLSDKESDRCHVNIVEKVQSIRSDHESYVTQFAKINNEVAICDRPGNDSENREITSLALSGIQLLCQWSCAVVETISWKLLNPTNPKDNRECPENAEEYERATRYNYSPAEKTALIQIIAMIKGLQSMLGKTESDMSNSTRKCVYVELQAFIHHTINEPLQKAVKHKKDLLASILQSVKDSISDAGNELNRMTDVKGKKKSSAPKGDSANSSSSDIRIPRRTAAPGSTQLYMARTQLESLISDKLCGGKKILRKELDSKTIEKISVFLRKSAHWPALFRLSDSMTEAGELSQLWFREFYLEMTMGQRIQFPIEMSMPWILTDYILSCNEPSLIESALYQLDLYNDAAQYSLFNFNKQFLYDEVEAEVNLCFDQFVYKLSEMVFTHYKQLASCMLLDKRFKAEILRSGTMIRSPSAARFESLLQQRHVQLLGRSVDLNRVVSQRVNMALLKALDAAIWKFESEPLSSIVELDMLIDTNRLCHTLLSDVLHSIAPFDDLFQEANHAVNSPHGRITLHVFWELNYDFVPNFVYNGSTHRFVRARHVFRKTPAREKPPQVGQVYYWGSKSLMAAFMNICNAYSQCIGTQHLKAITRLLHYQGIAVILDELLKMTNRLLNDKIRRHVRNVFNMMPKVCKLPRSDYGSNALLQYYVHHLEAVGKYPELKSEFCQDLRELGNMIVFCQQLEVALGQEEAHDLFLAAAYTGTVPQPPARNAQEQMKQLAKLEDKYSRIHLTEIIDKISPDDGQAAIAKDAELMTKERLCCGLNAFENFLVRIKQMLAADDIWTGGYPTNGVFWIDECVEWYRVYSALQFFLCQPTRDDNEVYAEELFGDSLQWGGLTLITLLGQHRRFEVLDFCYHLHRVNKADGKDEVISGIRLAKMVERIRRFQLLNNQIFIILENQLNENNDDPNERVREFAPPVHPNYANHAARRQ 34FBE7B43260A188 gex3-gex2-1 3 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 6A Using the yeast two-hybrid system, we found that the full-length GEX-2 could interact with the full-length GEX-3 (Fig. 6A). gex3-gex2-2 2 4 unspecified role unspecified role bait bait n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role prey prey n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 6B An interaction between GEX-2 and GEX-3 was also observed in yeast cells expressing HA-GEX-2 and myc-GEX-3 by reciprocal coimmunoprecipitation experiments (Fig. 6B). gex2-gex3-1 2 5 unspecified role unspecified role bait bait n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role prey prey n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 6B An interaction between GEX-2 and GEX-3 was also observed in yeast cells expressing HA-GEX-2 and myc-GEX-3 by reciprocal coimmunoprecipitation experiments (Fig. 6B). gex3-gex2-3 1 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Fig 6C Immunoprecipitation with the GEX-3 antibody coprecipitates a protein that is recognized by the GEX-2 antibody.