UniProt UniProt http://www.uniprot.org Analysis of Schizosaccharomyces pombe mediator reveals a set of essential subunits conserved between yeast and metazoan cells. Analysis of Schizosaccharomyces pombe mediator reveals a set of essential subunits conserved between yeast and metazoan cells. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2001 imex curation Spahr H., Samuelsen CO., Baraznenok V., Ernest I., Huylebroeck D., Remacle JE., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson CM. Proteins were separated by Bio-Rex 70, DEAE Sephacryl FF, and hydroxyapatite prior to fractionation over MonoQ, heparin and Sepharose columns. claes.gustafsson@mednut.ki.se 13-Nov-2007: Contacted by JYOTI. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast chromatography chromatography technology column chromatography chromatography fingerprinting peptide massfingerprinting fingerprinting Spahr H., Samuelsen CO., Baraznenok V., Ernest I., Huylebroeck D., Remacle JE., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson CM. Proteins were separated by Bio-Rex 70, DEAE Sephacryl FF, and hydroxyapatite prior to fractionation over MonoQ, heparin and Sepharose columns. claes.gustafsson@mednut.ki.se Accepted 2007-NOV-3 by JYOTI. 13-Nov-2007: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2001 Only protein-protein interactions imex curation Analysis of Schizosaccharomyces pombe mediator reveals a set of essential subunits conserved between yeast and metazoan cells. Analysis of Schizosaccharomyces pombe mediator reveals a set of essential subunits conserved between yeast and metazoan cells. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2001 imex curation Spahr H., Samuelsen CO., Baraznenok V., Ernest I., Huylebroeck D., Remacle JE., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson CM. Proteins were separated by Bio-Rex 70, DEAE Sephacryl FF, and hydroxyapatite prior to fractionation over MonoQ, heparin and Sepharose columns. claes.gustafsson@mednut.ki.se 13-Nov-2007: Contacted by JYOTI. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast chromatography chromatography technology column chromatography chromatography western blot western blot Immuno blot Immuno blot Spahr H., Samuelsen CO., Baraznenok V., Ernest I., Huylebroeck D., Remacle JE., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson CM. Proteins were separated by Bio-Rex 70, DEAE Sephacryl FF, and hydroxyapatite prior to fractionation over MonoQ and heparin HiTrap. claes.gustafsson@mednut.ki.se Accepted 2007-NOV-3 by JYOTI. 13-Nov-2007: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2001 Only protein-protein interactions imex curation Analysis of Schizosaccharomyces pombe mediator reveals a set of essential subunits conserved between yeast and metazoan cells. Analysis of Schizosaccharomyces pombe mediator reveals a set of essential subunits conserved between yeast and metazoan cells. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2001 imex curation Spahr H., Samuelsen CO., Baraznenok V., Ernest I., Huylebroeck D., Remacle JE., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson CM. Proteins were separated by Bio-Rex 70, DEAE Sephacryl FF, and hydroxyapatite prior to fractionation over MonoQ, heparin and Sepharose columns. claes.gustafsson@mednut.ki.se 13-Nov-2007: Contacted by JYOTI. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast chromatography chromatography technology column chromatography chromatography weight silver stain molecular weight estimation by silver staining weight silver stain Spahr H., Samuelsen CO., Baraznenok V., Ernest I., Huylebroeck D., Remacle JE., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson CM. Proteins were separated by Bio-Rex 70, DEAE Sephacryl FF, and hydroxyapatite prior to fractionation over MonoQ, heparin and Sepharose columns. claes.gustafsson@mednut.ki.se Accepted 2007-NOV-3 by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2001 Only protein-protein interactions imex curation Analysis of Schizosaccharomyces pombe mediator reveals a set of essential subunits conserved between yeast and metazoan cells. Analysis of Schizosaccharomyces pombe mediator reveals a set of essential subunits conserved between yeast and metazoan cells. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2001 imex curation Spahr H., Samuelsen CO., Baraznenok V., Ernest I., Huylebroeck D., Remacle JE., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson CM. Proteins were separated by Bio-Rex 70, DEAE Sephacryl FF, and hydroxyapatite prior to fractionation over MonoQ, heparin and Sepharose columns. claes.gustafsson@mednut.ki.se 13-Nov-2007: Contacted by JYOTI. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast chromatography chromatography technology column chromatography chromatography western blot western blot Immuno blot Immuno blot Spahr H., Samuelsen CO., Baraznenok V., Ernest I., Huylebroeck D., Remacle JE., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson CM. Proteins were separated by Bio-Rex 70, DEAE Sephacryl FF, and hydroxyapatite prior to fractionation over MonoQ, heparin HiTrap and Superose 12. claes.gustafsson@mednut.ki.se Accepted 2007-NOV-3 by JYOTI. 13-Nov-2007: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2001 Only protein-protein interactions imex curation Analysis of Schizosaccharomyces pombe mediator reveals a set of essential subunits conserved between yeast and metazoan cells. Analysis of Schizosaccharomyces pombe mediator reveals a set of essential subunits conserved between yeast and metazoan cells. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2001 imex curation Spahr H., Samuelsen CO., Baraznenok V., Ernest I., Huylebroeck D., Remacle JE., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson CM. Proteins were separated by Bio-Rex 70, DEAE Sephacryl FF, and hydroxyapatite prior to fractionation over MonoQ, heparin and Sepharose columns. claes.gustafsson@mednut.ki.se 13-Nov-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Spahr H., Samuelsen CO., Baraznenok V., Ernest I., Huylebroeck D., Remacle JE., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson CM. Mediator proteins were purified from S.pombe extracts by Bio-Rex 70, DEAE Sephacryl FF, and hydroxyapatite prior to fractionation over MonoQ, heparin HiTrap and Superose 12. claes.gustafsson@mednut.ki.se Accepted 2007-OCT-25 by JYOTI. 13-Nov-2007: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2001 Only protein-protein interactions imex curation rpab2_schpo DNA-directed RNA polymerases I, II, and III subunit RPABC2 rpb6 rpo15 SPCC1020.04c DNA-directed RNA polymerases I, II, and III 15 kDa polypeptide RPC16 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSDYEEDEAFGMDGAVMEEEVDELEMIDENGQSQQGVSHPGEPSTTVITEDVASSKTAQSGKAVAKEDRTTTPYMTKYERARILGTRALQISMNAPVLVDLEGETDPLQIAMKELAQKKIPLLVRRYLPDGSYEDWSVAELI FDC4E7AD5B495D79 med10_schpo Mediator of RNA polymerase II transcription subunit 10 med10 nut2 SPBC31F10.09c Mediator complex subunit 10 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MLPQDDMTDEMKSLASRLEDTTQAFYDLALIVYNLEDTTPSDAIPESLDTLIRDLKSLPDISRKVNNLIPQDVLEYIEQGRNPDVYARQFSELVQKDNQYVNGKLYAIEGFQKAFAEEIKQAYPEVSSVVDKILNEGKVESTVS A780C526591714C3 med7_schpo Mediator of RNA polymerase II transcription subunit 7 Mediator complex subunit 7 med7 SPBC14F5.08 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MEPNEGVQLFSAFPPPPPYYKLFTRENIEKVISNMEKEAKHEDDANTLQPKTEEEIESLAKLFKKPSCLTSGTYQMFGDTWRLDEAIPSLKEFGIPELYKDIKDGEDEIEVVEYDPKSNAIVGTSFTRVHDYDKNSPIENEKILEDDQHTAMKEKDNESDKTMKDVEEKTEPSLKKEEEDIQMKEPLDSQDTGAVSASSVNEGFRADQKSKDGETSDLIKIPRRAYELRFLSRSLMLNFLELLGIMAKAPEQFPSKVENIRVLLLNLHHLINDYRPHQSRESLIMLLEKQLKHEESQVELLRTHNRQMTETLEKYKSLDFNMEKEGDVIQQLKSSIKKPLSGAEDEQKSRSMFSKNDEKLKKSLELMEDVIKRDLS A2FFA4E9CFAAEE99 rpab3_schpo DNA-directed RNA polymerases I, II, and III subunit RPABC3 rpb8 SPBC14C8.12 DNA-directed RNA polymerases I, II, and III 14.5 kDa polypeptide RPC14 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSESVLLDEIFTVTSVDKQKYQRVSRITAVSGQNDMNLTLDINSQIYPLEKDATFSLQITSNLNSPDLKEAADYIMYGKVYRVEEAKDEKVSVYVSFGGLLMAIEGSHRKLYRLSLDHVYLLLRR 8941D1BB8D624770 med8_schpo Mediator of RNA polymerase II transcription subunit 8 med8 sep15 SPBC21.04 Mediator complex subunit 8 Cell separation protein sep15 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MEDISTEKTVESLEAIRHRIAQIVQSLTHFLAILHQSESLSPWPTIHKNFNILLSQIHSLSNNLAAHSHTLQTTSIYPSLEFPVKEQEPLLTTLLRTKALPEVEEWEANTLQEYEASISSQPKKKEANDAYQKDQLWDQARIIFMEERENYSWFDFVTRRQESEGEFVSQRQLEIDRATEEQNANQMLTDILSFMKSGKR A61A9E49B83A86F0 A61A9E49B83A86F0 med6_schpo Mediator of RNA polymerase II transcription subunit 6 med6 pmc5 SPAC1002.15c Mediator complex subunit 6 RNA polymerase II mediator complex protein pmc5 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MASGAPPSVDLTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQFNALDLGDLNSQLKRLTGIQFVIIHERPPFLWVIQKQNRLNENEVKPLTVYFVCNENIYMAPNAYTLLATRMLNATYCFQKALTKIEKFPQYNPQEGYTYPKLSNDNLEVDHSNTNEPADENKNQSIENADYSFSPEDFSVVRAFMQSLHSSKEAPDVK 84AE0C11F607F35A 84AE0C11F607F35A med19_schpo Mediator of RNA polymerase II transcription subunit 19 med19 rox3 SPCC1450.05c Mediator complex subunit 19 RNA polymerase II mediator complex protein rox3 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MAQAPEYHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQDLPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVMGNVSRDSSQPRSPSRRKK 32D1261667B27D52 rpb3_schpo DNA-directed RNA polymerase II subunit RPB3 DNA-directed RNA polymerase II 33 kDa polypeptide rpb3 SPCC1442.10c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDSETHITIRNISKNSVDFVLTNTSLAVANSLRRVVLAEIPTVAIDLVEINVNTSVMPDEFLAHRLGMIPLDSSNIDEPPPVGLEYTRNCDCDQYCPKCSVELFLNAKCTGEGTMEIYARDLVVSSNSSLGHPILADPKSRGPLICKLRKEQEISLRCIAKKGIAKEHAKWSPTSAVAFEYDPWNKLQHTDYWFENDADAEWPKSKNADWEEPPREGEPFNFQEEPRRFYMDVESVGSIPPNEIMVQGLRILQEKLAVLVRDLDEEQPTQLSANELNMEENAEMNWSPYQNGEENTW 94F639734B162DDF rpb4_schpo DNA-directed RNA polymerase II subunit rpb4 rpb4 SPBC337.14 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MPRAIFEEDAAQLKLGPEFENEDMLTVSEAKILIETVLAQRARETNGEIPMTDVMKKTVAYFNVFARFKTAEATYACERILGNRFHKFERAQLGTLCCEDAEEARTLIPSLANKIDDQNLQGILDELSTLRKFQD 61C98593A01C1E04 med21_schpo Mediator of RNA polymerase II transcription subunit 21 med21 srb7 SPBC1604.10 Mediator complex subunit 21 Suppressor of RNA polymerase B 7 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MACRCTQLQDTIDEVATQFYSSIHYLSSHHDFVPLPGQEKVSDSKVNPISAEELQFAQRDLAKDLVTKFMQIDTLINQLPGISTAPKHQLEKIKKLQNSIEEKQLERKSLESENEDLKLQLAKRIETFGRLSCVLFQP 5A7C236108C09AF9 rpb1_schpo DNA-directed RNA polymerase II subunit rpb1 DNA-directed RNA polymerase III largest subunit rpb1 SPBC28F2.12 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSGIQFSPSSVPLRRVEEVQFGILSPEEIRSMSVAKIEFPETMDESGQRPRVGGLLDPRLGTIDRQFKCQTCGETMADCPGHFGHIELAKPVFHIGFLSKIKKILECVCWNCGKLKIDSSNPKFNDTQRYRDPKNRLNAVWNVCKTKMVCDTGLSAGSDNFDLSNPSANMGHGGCGAAQPTIRKDGLRLWGSWKRGKDESDLPEKRLLSPLEVHTIFTHISSEDLAHLGLNEQYARPDWMIITVLPVPPPSVRPSISVDGTSRGEDDLTHKLSDIIKANANVRRCEQEGAPAHIVSEYEQLLQFHVATYMDNEIAGQPQALQKSGRPLKSIRARLKGKEGRLRGNLMGKRVDFSARTVITGDPNLSLDELGVPRSIAKTLTYPETVTPYNIYQLQELVRNGPDEHPGAKYIIRDTGERIDLRYHKRAGDIPLRYGWRVERHIRDGDVVIFNRQPSLHKMSMMGHRIRVMPYSTFRLNLSVTSPYNADFDGDEMNMHVPQSEETRAEIQEITMVPKQIVSPQSNKPVMGIVQDTLAGVRKFSLRDNFLTRNAVMNIMLWVPDWDGILPPPVILKPKVLWTGKQILSLIIPKGINLIRDDDKQSLSNPTDSGMLIENGEIIYGVVDKKTVGASQGGLVHTIWKEKGPEICKGFFNGIQRVVNYWLLHNGFSIGIGDTIADADTMKEVTRTVKEARRQVAECIQDAQHNRLKPEPGMTLRESFEAKVSRILNQARDNAGRSAEHSLKDSNNVKQMVAAGSKGSFINISQMSACVGQQIVEGKRIPFGFKYRTLPHFPKDDDSPESRGFIENSYLRGLTPQEFFFHAMAGREGLIDTAVKTAETGYIQRRLVKAMEDVMVRYDGTVRNAMGDIIQFAYGEDGLDATLVEYQVFDSLRLSTKQFEKKYRIDLMEDRSLSLYMENSIENDSSVQDLLDEEYTQLVADRELLCKFIFPKGDARWPLPVNVQRIIQNALQIFHLEAKKPTDLLPSDIINGLNELIAKLTIFRGSDRITRDVQNNATLLFQILLRSKFAVKRVIMEYRLNKVAFEWIMGEVEARFQQAVVSPGEMVGTLAAQSIGEPATQMTLNTFHYAGVSSKNVTLGVPRLKEILNVAKNIKTPSLTIYLMPWIAANMDLAKNVQTQIEHTTLSTVTSATEIHYDPDPQDTVIEEDKDFVEAFFAIPDEEVEENLYKQSPWLLRLELDRAKMLDKKLSMSDVAGKIAESFERDLFTIWSEDNADKLIIRCRIIRDDDRKAEDDDNMIEEDVFLKTIEGHMLESISLRGVPNITRVYMMEHKIVRQIEDGTFERADEWVLETDGINLTEAMTVEGVDATRTYSNSFVEILQILGIEATRSALLKELRNVIEFDGSYVNYRHLALLCDVMTSRGHLMAITRHGINRAETGALMRCSFEETVEILMDAAASGEKDDCKGISENIMLGQLAPMGTGAFDIYLDQDMLMNYSLGTAVPTLAGSGMGTSQLPEGAGTPYERSPMVDSGFVGSPDAAAFSPLVQGGSEGREGFGDYGLLGAASPYKGVQSPGYTSPFSSAMSPGYGLTSPSYSPSSPGYSTSPAYMPSSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSATSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPS 15A4F0B59E60E570 med18_schpo Mediator of RNA polymerase II transcription subunit 18 med18 pmc6 sep11 SPAC5D6.05 Mediator complex subunit 18 Cell separation protein sep11 RNA polymerase II mediator complex protein pmc6 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MQELYLLGVVPSRRFEAVVNSLSKTLDGPKTILEFWVVYRPKDVPPNLPRQPDSWLRLCSNIESHDETDTEWSKNTQWSMYLEGNSEPKREDKCGIRPVNRAKLTNGSVTEFVEKMGYEFSHEYIIQGLEYFFFDTTVRIYQTLIPSQQRSIKPPFHPMNEEQPWILHVYTHVADASNQVAMAKAEANLTKVKTLLSAFCDLKNVRL A3A3B518E3D85A62 A3A3B518E3D85A62 rpab1_schpo DNA-directed RNA polymerases I, II, and III subunit RPABC1 rpb5 SPAC23C4.15 RPC24B protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSAEEKNIVRVFRAWKTAHQLVHDRGYGVSQAELDLTLDQFKAMHCGMGRNLDRTTLSFYAKPSNDSNKGTIYIEFAKEPSVGIKEMRTFVHTLGDHNHKTGILIYANSMTPSAAKIIATVTGQFTIETFQESDLIVNITHHELVPKHILLSPDEKKELLDRYKLRETQLPRIQLADPVARYLGLKRGEVVKIVRRSETSGRYNSYRICA EB74C07B9F048762 rpb11_schpo DNA-directed RNA polymerase II subunit RPB11 rpb11 SPAC3A12.07 DNA-directed RNA polymerase II 14.1 kDa polypeptide protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MNQPERYELIELMGLPKVTYELDSKSPNAAVVTLEKEDHTLANMLANQLLSDERVLFAGYKVPHPLNHNFILRVQTVEDCSPKQVIVDAAKSLITHLEEIKVNFMREWELKMISVEGVEMEFS 73FAFDF2F468386E med1_schpo Mediator of RNA polymerase II transcription subunit 1 Mediator complex subunit 1 RNA polymerase II Mediator complex protein pmc2 med1 pmc2 SPAC2F7.04 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDRPHLFSEVHFRRNASNPNQINWTRYWIDCLSSVPWNDISVTGFEYLAKKYGLEVFQDSSKPNEVVLSLAGKIILIDITVPINASPKDINVVLAFANASGEQYNNPVAEKLLKDAIYNCNTPLFEKNVKWLATFDHSSPSVQQSCFQYLDSLSSSLNAIYEAELSLLAQEENVIMHGNGKPLSNYEGQLGLRIVYWKLLDKTYSTQIFMDNLSHESLPHLLFGYNLLNSPPILQSSKINWALENTLTPIPTTMELVFDDINLIIPEQGVKPLLDLLHVHDITIPWPVQNYSHMLGLPSKSTVNYKFINKNQAIQLTGFDVSARSLRHVPFHHPKQIRGILAIVRQYLLLQLILENIKSADLAETAASSSLHLSLYFKEHPIVHAQYQEINKLNNSEQIIIVLSVLSDGRLNVDYMSSGGKELSKQQSHVFQKLIQQTCNIGLAIEVFIKKVVN C6366431E1E6758C rpb2_schpo DNA-directed RNA polymerase II subunit RPB2 DNA-directed RNA polymerase II 138 kDa polypeptide rpb2 SPAC23G3.01 SPAC521.06 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSYEDYQYNETLTQEDCWTVISSFFEETSLARQQLFSFDEFVQNTMQEIVDDDSTLTLDQYAQHTGAQGDVTRRYEINFGQIYLSRPTMTEADGSTTTMFPQEARLRNLTYSSPLYVDMRKKVMVAADSNVPIGEEEWLVEEEDEEPSKVFIGKIPIMLRSTFCILNGVSDSELYDLNECPYDQGGYFIINGSEKVIIAQERSAANIVQVFKKAAPSPIAYVAEIRSALERGSRLISSMQIKLMARNTENSGQTIRATLPYIRSDIPIVIVFRALGVVPDRDILEHICYDPNDFQMLEMMKPCIEEAFVIQDKDIALDYIGKRGSTTGVTREKRLRYAHDILQKELLPHITTMEGFETRKAFFLGYMIHRMLLCALERREPDDRDHFGKKRLDLAGPLLASLFRMLFRKMTRDVYKYMQKCVETNREFNLTLAVKSNIITNGLRYSLATGNWGDQKRSMVNRVGVSQVLNRYTFASTLSHLRRTNTPIGRDGKLAKPRQLHNTHWGMVCPAETPEGQACGLVKNLSLMSYVSVGSPSAPIIEFLEEWGLETLEDYNPSASPNATKVFVNGVWLGVHRDPAHLTETLRSLRRRLDISAEVSIVRDIREKELRLFTDAGRICRPLFIVDNNPNSERRGELCIRKEHIQQLIEDKDRYDIDPEQRFGWTALVSSGLIEYLDAEEEETVMIAMSPEDLEASRQMQAGYEVKEELDPAQRVKPAPNPHVHAWTHCEIHPAMILGILASIIPFPDHNQSPRNTYQSAMGKQAMGVYLTNYQVRMDTMANILYYPQKPLATTRSMEYLKFRELPAGQNAIVAILCYSGYNQEDSIIMNQASIDRGLFRSIFYRTYTDQEKKIGMTVMEEFERPVRSTTLRMKHGTYDKLEDDGLIAPGTRVSGEDIIIGKTAPIPLDHEELGQRTQLHAKRDVSTPLRSTESGIVDQVMVTTNQEGLKFVKVRMRSTRIPQIGDKFASRHGQKGTIGMTYRHEDMPFSAQGIVPDIIINPHAIPSRMTVAHLVECQLSKVSALSGFEGDATPFTDVTVEAVSKLLRSHGFQSRGFEVMYHGHTGRKLVAQVFLGPTYYQRLKHLVDDKIHARARGPVQILTRQPVEGRSRDGGLRFGEMERDCQISHGCSSVLRERLFDCSDAYRVIVCDICGLIAIASYKKDSYECRSCQNRTRFSQVYLPYAAKLLFQELMSMNIAPRLFTKNHK C5D3794A743494CC med27_schpo Mediator of RNA polymerase II transcription subunit 27 med27 pmc3 SPAC17C9.05c Mediator complex subunit 27 RNA polymerase II mediator complex protein pmc3 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSLEEQRTRDELKHKLLDLNQLHEQLAELRTICPSLLKLLHPETGTSRKFEKSAQEAIEKVNSFYTHLKSSQNVFDYAEKSLQADSSNLLPTYLYNSEDLSNDTENNETKSINGKSALDLKEPHHSELHDNDNFQNSDINIESFKGDIEASGSILTTHENKSFTLKLANELEFIFFHDTRGKFSVYCSSSKDDAITFSINRNNNFLGNLWSLMPKILDYQHLYSKPCDFCKSLISPVYLELPSVRRNANSTVKPTSKDILALHAECVPAQSDL C88A085C6BFF702C med17_schpo Mediator of RNA polymerase II transcription subunit 17 med17 srb4 SPBC31F10.04c Mediator complex subunit 17 Suppressor of RNA polymerase B 4 homolog protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MAEEANKDADISSLSLSLDPEIIGGQNNFLENNLQQIFQKIIQERGPFRDLKEEDLQKELQKESIKDESSAKSSETENVLEFATLDSKRNVNDTEVESMDSQAYKKELIEQIMIAQTECSLALDMTSLLLSKFKENSIETISPFLKSTVPPSSLQFSRSQPPESKESDATLAKCWKEKSLTSSCKFLFEAKERLTSVVETEHEYYTELVKVKEASWPLFNSQGSNHLSVQYSCLGGISLGLGLIRMKPESKSFEVQSSLLYSQAALKISILNKDRDEIGSSTWSWPSQNCNSVLLKDIYKLQEILFEMDIWNSLLQEAQSCGNQGVNFTGDEILVPISDDHVVRITLETSSKNTESGFTEDKKSNEDTSTNFVTIKQEKELLKCLCDTLNAIAHILFLKHCRKSDRRSQQPELYMAIDANAPLILRPLIFYYNLNQESLEFQRWLKQRDISFKFMPNYPWEKAKDFLELENSLSINRLSISWRIMVSNFEPAIFIQHTPTLHGTDKSVWRCKDQYSSNQFSSLKNVCQYIEHHINSLSRRSKKTE 5BC16074D9D0A05B 5BC16074D9D0A05B med14_schpo Mediator of RNA polymerase II transcription subunit 14 Mediator complex subunit 14 Mediator of RNA polymerase II complex subunit pmc1 med14 pmc1 SPBC1A4.10c SPBP23A10.01c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MEPPAIPHITEGFYPLPEIVETFSHHVLQELVSLAEVLPSMSNVEKKKKILDWLLRSRAFTMRLLVLARWVHLSPSVHRCIDVVAFLQGQKFCFQNLVHVLQDIRYQLSFARLRNSDLVTALDILSTGTSLRLANAPTSKLYMLSESPLSTKQILQTLHALNMLIRIRLSLYEIIPTPFQHFTIANGRCTFTVPNEFSVSLTTNSQDPKSTGISFQWIVVDFQFHLPDFSSTPAKYRVFIELHLNEEIAAAFVLQKPILPLIYNILHKFCLYQRLNLLSQQTFQLSRESWLGHLRGVYDEKPPRLRLYYWPQLNVKKEGKPGKIGHYIHIFVNTQPISAFERTLSSKRSSCEYDHFLLLVEWHHDGIVEHVPLDDHMDAQHLLLLITQKHAQLILEQIRKELHPNIFSEHVGGGLKIHVFDNEIIVKVNSVTGRLVLSSSASPLSPPRHLRAAEKNIALNTQPPAQILNRLYFFCIQTQLLEVAQCAELHAVQGYYSFPYLTFSKGKWRKDGDSLWVLAYNVESNSWSVRLLNAAGQTLYTQDVHTTKGTLSIESFSRLSYLLEVQILLFNVQTACQARGMPFEYLPIPPKALIEDDFTTYVQTGCLCIMMPSSNEDMLPVVFVRAHDGQLIFDSRIKGKLPYQSETETEKNCYIDWRTGRITIRVQNFSSFEKTWIGLLKLVALSKTSAFNVDCITLKHVDFTYLDDEKFRATIHDDNTFTLHFFNRHSPFHLISQFLQDTFSDGPSAIQPLRVIMDRTRGVLVAQELGYVVLARSLRQYRIILSKNHGIQVLLNRHGCILQDLSYLSADSRYLEGTQTLTSQWEPCSWLNTVWEGDLGDDELNGQIEAAPEMHLIKMNKTADLTAILKRILAISRKK C6449D586FB613E8 rpab4_schpo DNA-directed RNA polymerases I, II, and III subunit RPABC4 SPBC19C2.03 rpc10 rpb12 ABC10-alpha protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MNHPTSTGGTAFNPPRPATMIYLCADCGARNTIQAKEVIRCRECGHRVMYKMRTKRMVQFEAR 97EBE50FAA95E884 med22_schpo Mediator of RNA polymerase II transcription subunit 22 med22 srb6 SPAC29A4.07 Mediator complex subunit 22 Suppressor of RNA polymerase B 6 homolog protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSSDSFQRQLVQRTNTLNSSIDNATLTILSRFQDILDIAINEGKDKYTVAPEVYQIECHTVSMVRAVEQLLDVSRQIKSYWLTNSLSTSFPTVDYSEPDLEKVKRTLTKLQNHLLEVSLIEPEASETTEAPTVSDT 52EA5A74C06C9FE1 52EA5A74C06C9FE1 rpb7_schpo DNA-directed RNA polymerase II subunit rpb7 rpb7 SPACUNK4.06c SPAPYUK71.02 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MPFFLKELSLTISLHPSYFGPRMQDYLKAKLLADVEGTCSGQYGYIICVLDSNTIDIDKGRVVPGQGFAEFEVKYRAVLWRPFRGEVVDAIVTTVNKMGFFANIGPLNVFVSSHLVPPDMKFDPTANPPNYSGEDQVIEKGSNVRLKIVGTRTDATEIFAIATMKEDYLGVL 0AECC46B9499FD33 vp16_hhv2h Tegument protein VP16 Alpha trans-inducing protein UL48 Vmw65 ICP25 Alpha-TIF protein protein hhv2h Human herpesvirus 2 (strain HG52) (HHV-2) Human herpes simplex virus 2 MDLLVDDLFADADGVSPPPPRPAGGPKNTPAAPPLYATGRLSQAQLMPSPPMPVPPAALFNRLLDDLGFSAGPALCTMLDTWNEDLFSGFPTNADMYRECKFLSTLPSDVIDWGDAHVPERSPIDIRAHGDVAFPTLPATRDELPSYYEAMAQFFRGELRAREESYRTVLANFCSALYRYLRASVRQLHRQAHMRGRNRDLREMLRTTIADRYYRETARLARVLFLHLYLFLSREILWAAYAEQMMRPDLFDGLCCDLESWRQLACLFQPLMFINGSLTVRGVPVEARRLRELNHIREHLNLPLVRSAAAEEPGAPLTTPPVLQGNQARSSGYFMLLIRAKLDSYSSVATSEGESVMREHAYSRGRTRNNYGSTIEGLLDLPDDDDAPAEAGLVAPRMSFLSAGQRPRRLSTTAPITDVSLGDELRLDGEEVDMTPADALDDFDLEMLGDVESPSPGMTHDPVSYGALDVDDFEFEQMFTDAMGIDDFGG 2E9FDA8D0D8BC174 med4_schpo Mediator of RNA polymerase II transcription subunit 4 med4 pmc4 SPBC1105.06 Mediator complex subunit 4 RNA polymerase II mediator complex protein pmc4 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MEYQRAIDSIEECLNKQLRLSSEKVDQYVLIENWTSLVGHLKTLHSLISNYTNGRELQNEISSLLKQDKELDLQIQDCMREMTSIYDTHLPKTVSGRKRQKVNAETLLDYGRKLSKFSSAPPGYNPETGQDAKAPVHYPWPSEDQMRKTLLFQFSTSMVPNLSATASQLFSEQPPKTNEPTETETEIDANKAVEEKTKMNYPASPTFTTQEENKEVESPANKDVFAGFDLFDPEMEEDF AE026602067F0DB3 med4-med6-1 3 20 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component 23 unspecified role unspecified role neutral component neutral component 24 unspecified role unspecified role neutral component neutral component 25 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component 26 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component 17 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component 29 unspecified role unspecified role neutral component neutral component 22 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component 21 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component 27 unspecified role unspecified role neutral component neutral component physical association physical association Fig 1A, 1B med4-med6-2 1 17 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component 23 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component 26 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component 20 unspecified role unspecified role neutral component neutral component 22 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 29 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component 24 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component physical association physical association Table 1 med17-med27-1 2 23 unspecified role unspecified role neutral component neutral component 17 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component 22 unspecified role unspecified role neutral component neutral component physical association physical association Fig 2A med17-med27-2 4 22 unspecified role unspecified role neutral component neutral component 23 unspecified role unspecified role neutral component neutral component 17 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component physical association physical association Fig 2B vp16-med17 5 23 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 7 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 28 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P68336:p.[Phe443Pro;Phe473Ala;Phe475Ala;Phe479Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain F P certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A Wed May 02 18:21:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe443pro,phe473ala,phe475ala,phe479ala' Tue Jun 30 19:19:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe443Pro;Phe473Ala;Phe475Ala;Phe479Ala]' ecobb Escherichia coli (strain B / BL21) association association Fig. 4 med1-med4-1 2 7 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component 17 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component 20 unspecified role unspecified role neutral component neutral component 29 unspecified role unspecified role neutral component neutral component 22 unspecified role unspecified role neutral component neutral component 23 unspecified role unspecified role neutral component neutral component 24 unspecified role unspecified role neutral component neutral component 27 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component 21 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component physical association physical association Table 1