IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Subcellular localization and oligomerization of the Arabidopsis thaliana somatic embryogenesis receptor kinase 1 protein. Subcellular localization and oligomerization of the Arabidopsis thaliana somatic embryogenesis receptor kinase 1 protein. J. Mol. Biol. (0022-2836) 2001 imex curation Shah K., Gadella TW., van Erp H., Hecht V., de Vries SC. Sacco.deVries@mac.mb.wau.nl Only protein-protein interactions vigun Vigna unguiculata (Cowpea) fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Shah K., Gadella TW., van Erp H., Hecht V., de Vries SC. All experiments were based on fluorescence spectral imaging microscopy (FSPIM)as a detection system. Sacco.deVries@mac.mb.wau.nl Accepted 2007-DEC-23 by JYOTI. J. Mol. Biol. (0022-2836) 2001 Only protein-protein interactions imex curation Subcellular localization and oligomerization of the Arabidopsis thaliana somatic embryogenesis receptor kinase 1 protein. Subcellular localization and oligomerization of the Arabidopsis thaliana somatic embryogenesis receptor kinase 1 protein. J. Mol. Biol. (0022-2836) 2001 imex curation Shah K., Gadella TW., van Erp H., Hecht V., de Vries SC. Sacco.deVries@mac.mb.wau.nl Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Shah K., Gadella TW., van Erp H., Hecht V., de Vries SC. Sacco.deVries@mac.mb.wau.nl Accepted 2007-DEC-23 by JYOTI. J. Mol. Biol. (0022-2836) 2001 Only protein-protein interactions imex curation serk1_arath Somatic embryogenesis receptor kinase 1 SERK1 Somatic embryogenesis receptor-like kinase 1 F14O23.21 F14O23_24 At1g71830 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MESSYVVFILLSLILLPNHSLWLASANLEGDALHTLRVTLVDPNNVLQSWDPTLVNPCTWFHVTCNNENSVIRVDLGNAELSGHLVPELGVLKNLQYLELYSNNITGPIPSNLGNLTNLVSLDLYLNSFSGPIPESLGKLSKLRFLRLNNNSLTGSIPMSLTNITTLQVLDLSNNRLSGSVPDNGSFSLFTPISFANNLDLCGPVTSHPCPGSPPFSPPPPFIQPPPVSTPSGYGITGAIAGGVAAGAALLFAAPAIAFAWWRRRKPLDIFFDVPAEEDPEVHLGQLKRFSLRELQVASDGFSNKNILGRGGFGKVYKGRLADGTLVAVKRLKEERTPGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASCLRERPPSQPPLDWPTRKRIALGSARGLSYLHDHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGIMLLELITGQRAFDLARLANDDDVMLLDWVKGLLKEKKLEMLVDPDLQTNYEERELEQVIQVALLCTQGSPMERPKMSEVVRMLEGDGLAEKWDEWQKVEILREEIDLSPNPNSDWILDSTYNLHAVELSGPR DIP protein Q94AG2 original sequence version: 112 DIP protein Q94AG2 original sequence version: 115 98F6B69126DB664A serk1 2 3 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association There was no interaction between the two intracellular kinase domains of AtSERK1 (AtSERK1(266-625) in Figure 6) whereas the AtSERK1 extracellular domains do interact with each other (AtSERK1(26-234) in Figure 6) as indicated by the b-galactosidase activity and the growth without leucine. 6 serk1-1 1 3 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role neutral component neutral component region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction In case of FRET, the CFP �uorescence will be quenched and the YFP �uorescence would be increased (sensitized). Assuming approximately equal expression levels of both AtSERK1-CFP (Figure 1(c)) and -YFP (Figure 1(d)), we consider a ratio of �uorescence intensity at 525 nm over 475 nm (designated as the YFP/CFP emission ratio) of 1.3 or higher as evidence of FRET and hence of AtSERK1-AtSERK1 interactions such as oligomerization or homodimerization. 7 The authors conclude "receptor oligomerization is not uniformly distributed within the membrane of a single protoplast."