UniProt UniProt http://www.uniprot.org Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting. Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting. J. Biol. Chem. (0021-9258) 2000 imex curation Scott SV., Nice DC., Nau JJ., Weisman LS., Kamada Y., Keizer-Gunnink I., Funakoshi T., Veenhuis M., Ohsumi Y., Klionsky DJ. djklionsky@ucdavis.edu 07-Aug-2007: Contacted by JYOTI. Yeast Y2HL-C1 davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Scott SV., Nice DC., Nau JJ., Weisman LS., Kamada Y., Keizer-Gunnink I., Funakoshi T., Veenhuis M., Ohsumi Y., Klionsky DJ. didnot include subcellular fractioning expertiments fig. 7 VAC8 and APG13 have been overexpressed. In an additional experiment, cells have been treated with rapamycin. djklionsky@ucdavis.edu Accepted 2007-AUG-25 by DAVET. 07-Aug-2007: Contacted by JYOTI. Yeast Y2HL-C1 J. Biol. Chem. (0021-9258) 2000 davet Only protein-protein interactions imex curation Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting. Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting. J. Biol. Chem. (0021-9258) 2000 imex curation Scott SV., Nice DC., Nau JJ., Weisman LS., Kamada Y., Keizer-Gunnink I., Funakoshi T., Veenhuis M., Ohsumi Y., Klionsky DJ. djklionsky@ucdavis.edu 07-Aug-2007: Contacted by JYOTI. Yeast Y2HL-C1 davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Scott SV., Nice DC., Nau JJ., Weisman LS., Kamada Y., Keizer-Gunnink I., Funakoshi T., Veenhuis M., Ohsumi Y., Klionsky DJ. djklionsky@ucdavis.edu Accepted 2007-AUG-25 by DAVET. Yeast Y2HL-C1 J. Biol. Chem. (0021-9258) 2000 davet Only protein-protein interactions imex curation Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting. Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting. J. Biol. Chem. (0021-9258) 2000 imex curation Scott SV., Nice DC., Nau JJ., Weisman LS., Kamada Y., Keizer-Gunnink I., Funakoshi T., Veenhuis M., Ohsumi Y., Klionsky DJ. djklionsky@ucdavis.edu 07-Aug-2007: Contacted by JYOTI. Yeast Y2HL-C1 davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Scott SV., Nice DC., Nau JJ., Weisman LS., Kamada Y., Keizer-Gunnink I., Funakoshi T., Veenhuis M., Ohsumi Y., Klionsky DJ. djklionsky@ucdavis.edu Accepted 2007-AUG-25 by DAVET. 07-Aug-2007: Contacted by JYOTI. Yeast Y2HL-C1 J. Biol. Chem. (0021-9258) 2000 davet Only protein-protein interactions imex curation vac8_yeast Vacuolar protein 8 VAC8 YEB3 YEL013W protein protein yeast Saccharomyces cerevisiae Baker's yeast MGSCCSCLKDSSDEASVSPIADNEREAVTLLLGYLEDKDQLDFYSGGPLKALTTLVYSDNLNLQRSAALAFAEITEKYVRQVSREVLEPILILLQSQDPQIQVAACAALGNLAVNNENKLLIVEMGGLEPLINQMMGDNVEVQCNAVGCITNLATRDDNKHKIATSGALIPLTKLAKSKHIRVQRNATGALLNMTHSEENRKELVNAGAVPVLVSLLSSTDPDVQYYCTTALSNIAVDEANRKKLAQTEPRLVSKLVSLMDSPSSRVKCQATLALRNLASDTSYQLEIVRAGGLPHLVKLIQSDSIPLVLASVACIRNISIHPLNEGLIVDAGFLKPLVRLLDYKDSEEIQCHAVSTLRNLAASSEKNRKEFFESGAVEKCKELALDSPVSVQSEISACFAILALADVSKLDLLEANILDALIPMTFSQNQEVSGNAAAALANLCSRVNNYTKIIEAWDRPNEGIRGFLIRFLKSDYATFEHIALWTILQLLESHNDKVEDLVKNDDDIINGVRKMADVTFERLQRSGIDVKNPGSNNNPSSNDNNSNNNDTGSEHQPVEDASLELYNITQQILQFLH B2E75774B47E5933 B2E75774B47E5933 atg13_yeast Autophagy-related protein 13 ATG13 APG13 YPR185W protein protein yeast Saccharomyces cerevisiae Baker's yeast MVAEEDIEKQVLQLIDSFFLKTTLLICSTESSRYQSSTENIFLFDDTWFEDHSELVSELPEIISKWSHYDGRKELPPLVVETYLDLRQLNSSHLVRLKDHEGHLWNVCKGTKKQEIVMERWLIELDNSSPTFKSYSEDETDVNELSKQLVLLFRYLLTLIQLLPTTELYQLLIKSYNGPQNEGSSNPITSTGPLVSIRTCVLDGSKPILSKGRIGLSKPIINTYSNALNESNLPAHLDQKKITPVWTKFGLLRVSVSYRRDWKFEINNTNDELFSARHASVSHNSQGPQNQPEQEGQSDQDIGKRQPQFQQQQQPQQQQQQQQQQQRQHQVQTQQQRQIPDRRSLSLSPCTRANSFEPQSWQKKVYPISRPVQPFKVGSIGSQSASRNPSNSSFFNQPPVHRPSMSSNYGPQMNIEGTSVGSTSKYSSSFGNIRRHSSVKTTENAEKVSKAVKSPLQPQESQEDLMDFVKLLEEKPDLTIKKTSGNNPPNINISDSLIRYQNLKPSNDLLSEDLSVSLSMDPNHTYHRGRSDSHSPLPSISPSMHYGSLNSRMSQGANASHLIARGGGNSSTSALNSRRNSLDKSSNKQGMSGLPPIFGGESTSYHHDNKIQKYNQLGVEEDDDDENDRLLNQMGNSATKFKSSISPRSIDSISSSFIKSRIPIRQPYHYSQPTTAPFQAQAKFHKPANKLIDNGNRSNSNNNNHNGNDAVGVMHNDEDDQDDDLVFFMSDMNLSKEG 45CD16F69B795E62 45CD16F69B795E62 vac8-atg13-1 2 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association No figure vac8-atg13-2 3 5 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role bait bait physical association physical association 1 vac8-atg13-3 1 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 3 Treatment with rapamycin did not affect the interaction. overexpressed ATG13