UniProt UniProt http://www.uniprot.org Saccharomyces cerevisiae RAI1 (YGL246c) is homologous to human DOM3Z and encodes a protein that binds the nuclear exoribonuclease Rat1p. Saccharomyces cerevisiae RAI1 (YGL246c) is homologous to human DOM3Z and encodes a protein that binds the nuclear exoribonuclease Rat1p. Mol. Cell. Biol. 20(4006-4015) 2000 imex curation Xue Y., Bai X., Lee I., Kallstrom G., Ho J., Brown J., Stevens A., Johnson AW. arlen@mail.utexas.edu 22-Jan-2008: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xue Y., Bai X., Lee I., Kallstrom G., Ho J., Brown J., Stevens A., Johnson AW. arlen@mail.utexas.edu Accepted 2007-Not a month!-5 by ORCHARD. Mol. Cell. Biol. 20(4006-4015) 2000 orchard Only protein-protein interactions imex curation Saccharomyces cerevisiae RAI1 (YGL246c) is homologous to human DOM3Z and encodes a protein that binds the nuclear exoribonuclease Rat1p. Saccharomyces cerevisiae RAI1 (YGL246c) is homologous to human DOM3Z and encodes a protein that binds the nuclear exoribonuclease Rat1p. Mol. Cell. Biol. 20(4006-4015) 2000 imex curation Xue Y., Bai X., Lee I., Kallstrom G., Ho J., Brown J., Stevens A., Johnson AW. arlen@mail.utexas.edu 22-Jan-2008: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Xue Y., Bai X., Lee I., Kallstrom G., Ho J., Brown J., Stevens A., Johnson AW. The assay was based on the release of acid-soluble radioactivity from 32P-labeled tobacco acid pyrophosphatase-treated MFA2 transcript. arlen@mail.utexas.edu Accepted 2007-Not a month!-5 by ORCHARD. 22-Jan-2008: Contacted by JYOTI. Mol. Cell. Biol. 20(4006-4015) 2000 orchard Only protein-protein interactions imex curation xrn2_yeast 5'-3' exoribonuclease 2 Ribonucleic acid-trafficking protein 1 TAP1 YOR048C RAT1 HKE1 p116 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGVPSFFRWLSRKYPKIISPVLEEQPQIVDGVILPLDYSASNPNGELDNLYLDMNGIVHPCSHPENKPPPETEDEMLLAVFEYTNRVLNMARPRKVLVMAVDGVAPRAKMNQQRARRFRSARDAQIENEAREEIMRQREEVGEIIDDAVRNKKTWDSNAITPGTPFMDKLAAALRYWTAFKLATDPGWKNLQVIISDATVPGEGEHKIMNFIRSQRADPEYNPNTTHCIYGLDADLIFLGLATHEPHFKILREDVFAQDNRKRNNLKDTINMTEEEKQFLQKQNSEQPFLWLHINVLREYLSAELWVPGLPFTFDLERAIDDWVFMCFFCGNDFLPHLPCLDVRENSIDILLDIWKVVLPKLKTYMTCDGVLNLPSVETLLQHLGSREGDIFKTRHIQEARKKEAFERRKAQKNMSKGQDRHPTVATEQLQMYDTQGNLAKGSWNLTTSDMVRLKKELMLANEGNEEAIAKVKQQSDKNNELMKDISKEEIDDAVSKANKTNFNLAEVMKQKIINKKHRLEKDNEEEEIAKDSKKVKTEKAESECDLDAEIKDEIVADVNDRENSETTEVSRDSPVHSTVNVSEGPKNGVFDTDEFVKLFEPGYHERYYTAKFHVTPQDIEQLRKDMVKCYIEGVAWVLMYYYQGCASWNWFYPYHYAPLATDFHGFSHLEIKFEEGTPFLPYEQLMSVLPAASGHALPKIFRSLMSEPDSEIIDFYPEEFPIDMNGKKMSWQGIALLPFIDQDRLLTAVRAQYPLLSDAERARNIRGEPVLLISNKNANYERFSKKLYSKENNNNNVVVKFQHFKSGLSGIVSKDVEGFELNGKIVCPIQGGSLPNLSTTLILKMSYRLIPLPSRNKSIILNGFIPSEPVLTAYDLDSIMYKYNNQNYSRRWNFGNDLKQNIVPVGPKGITQYKPRTGGYRAFFYFAELSRNNVQPAHNYGRNSYNSQPGFNNSRYDGGNNNYRQNSNYRNNNYSGNRNSGQYSGNSYSRNNKQSRYDNSRANRR 5DDD5B0245F3E12A mfa2_yeast_mrna mrna messenger rna mRNA mrna yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast RNA strand derived from in vitro transcription of yeast Mfa2 DNA dxo_yeast Decapping nuclease RAI1 RAI1 RAT1-interacting protein NRE387 YGL246C NAD-capped RNA hydrolase RAI1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGVSANLFVKQRGSTTALKQPKEIGFYSRTKDEEYLISDDTNLNYYYLPDAELDRKLDLSSGFQKFKDYYKDFEDRCSLRGLLETIESSERHKGKKINADIITFRGIARKLISCAFDSPSFNTVDLRIVSFNGQLFIKEVPEAVNAAKASSATEAGRNINQDLNVFTGYKFETLATLSNPLQYTPREVIEKRTKRIVSHGDEYISVVRTGVGNCKLILGAEVDCIFDFKENGRDNLKHYAELKCTQQVANISDTHKFERKLFRTWLQCFLVGIPRIIYGFKDDHYVLKTVEEFSTEEVPVLLKNNNPQVGSACLEAIKWYGLLTEWLLKMIPRDEDPHSQIRAFKLVFENNHLRLSEIEESDEEYSGLIDGEHILSNGFKEWRKSLK E4A6565AF690A3E1 E4A6565AF690A3E1 rai1-rat1 1 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 7 Rai1p binds to Rat1p. rai1-rat1-1 2 5 enzyme enzyme neutral component neutral component n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 3 stimulator stimulator ancillary ancillary yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 4 enzyme target enzyme target substrate substrate neutral component neutral component enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction Fig. 9 & 10 Rai1p stabilizes the exoribonuclease activity of Rat1p in vitro. Rai1p stabilizes the exoribonuclease activity of Rat1p in vitro.