UniProt UniProt http://www.uniprot.org Caenorhabditis elegans mediator complexes are required for developmental-specific transcriptional activation. Caenorhabditis elegans mediator complexes are required for developmental-specific transcriptional activation. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 imex curation Kwon JY., Park JM., Gim BS., Han SJ., Lee J., Kim YJ. yjkim@smc.samsung.co.kr leej@yonsei.ac.kr 12-Oct-2007: Contacted by JYOTI. Only protein-protein interactions caeel Caenorhabditis elegans anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kwon JY., Park JM., Gim BS., Han SJ., Lee J., Kim YJ. Proteins were purified by heparin chromatography prior to immunoprecipitation. yjkim@smc.samsung.co.kr yjkim@smc.samsung.co.kr Accepted 2007-OCT-25 by JYOTI. 12-Oct-2007: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 Only protein-protein interactions imex curation Caenorhabditis elegans mediator complexes are required for developmental-specific transcriptional activation. Caenorhabditis elegans mediator complexes are required for developmental-specific transcriptional activation. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 imex curation Kwon JY., Park JM., Gim BS., Han SJ., Lee J., Kim YJ. yjkim@smc.samsung.co.kr leej@yonsei.ac.kr 12-Oct-2007: Contacted by JYOTI. Only protein-protein interactions caeel Caenorhabditis elegans anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kwon JY., Park JM., Gim BS., Han SJ., Lee J., Kim YJ. yjkim@smc.samsung.co.kr yjkim@smc.samsung.co.kr Accepted 2007-OCT-25 by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 Only protein-protein interactions imex curation Caenorhabditis elegans mediator complexes are required for developmental-specific transcriptional activation. Caenorhabditis elegans mediator complexes are required for developmental-specific transcriptional activation. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 imex curation Kwon JY., Park JM., Gim BS., Han SJ., Lee J., Kim YJ. yjkim@smc.samsung.co.kr leej@yonsei.ac.kr 12-Oct-2007: Contacted by JYOTI. Only protein-protein interactions caeel Caenorhabditis elegans ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom western blot western blot Immuno blot Immuno blot Kwon JY., Park JM., Gim BS., Han SJ., Lee J., Kim YJ. yjkim@smc.samsung.co.kr yjkim@smc.samsung.co.kr Accepted 2007-OCT-25 by JYOTI. 12-Oct-2007: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 Only protein-protein interactions imex curation Caenorhabditis elegans mediator complexes are required for developmental-specific transcriptional activation. Caenorhabditis elegans mediator complexes are required for developmental-specific transcriptional activation. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 imex curation Kwon JY., Park JM., Gim BS., Han SJ., Lee J., Kim YJ. yjkim@smc.samsung.co.kr leej@yonsei.ac.kr 12-Oct-2007: Contacted by JYOTI. Only protein-protein interactions caeel Caenorhabditis elegans molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Kwon JY., Park JM., Gim BS., Han SJ., Lee J., Kim YJ. A Superose-6 column was used. yjkim@smc.samsung.co.kr yjkim@smc.samsung.co.kr Accepted 2007-OCT-25 by JYOTI. 12-Oct-2007: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 Only protein-protein interactions imex curation med21_caeel Mediator of RNA polymerase II transcription subunit 21 mdt-21 C24H11.9 Mediator complex subunit 21 CeSRB7 protein protein caeel Caenorhabditis elegans MADRMTQLQDMINEMAGLMTNAIGVLQATAPPCEFGAISQELEDEPNCAIFAAGIAKAAKNIEILIDSFPIEAGNQEAEVEEKMIKNDEKQREKVNELVGLVGDSNRLVGVVQKKLAEISKTQMSSRPSE 1308EACEAD197C4B med10_caeel Mediator of RNA polymerase II transcription subunit 10 T09A5.6 mdt-10 Mediator complex subunit 10 CeMED10 CeNUT2 protein protein caeel Caenorhabditis elegans MVQQQQQSQQRMMELHERNDREKLARKTEKEREEERRKQEDDKILQLEKKLEEFQENARFIGDLASNFQTKYQDALNGRIYTLVRGLQDLDRMKGTFSDKKVPLDLLPYLDDGKNPCLYSKHCMEKTLEKNKAVNGKIEIYKKFRAHLMKEFSEEMPDLVMYYRSIREDLDLS 928DCB94C1DD2E1A rpb1_caeel DNA-directed RNA polymerase II subunit RPB1 DNA-directed RNA polymerase III largest subunit ama-1 F36A4.7 rpb-1 protein protein caeel Caenorhabditis elegans MALVGVDFQAPLRIVSRVQFGILGPEEIKRMSVAHVEFPEVYENGKPKLGGLMDPRQGVIDRRGRCMTCAGNLTDCPGHFGHLELAKPVFHIGFLTKTLKILRCVCFYCGRLLIDKSAPRVLEILKKTGTNSKKRLTMIYDLCKAKSVCEGAAEKEEGMPDDPDDPMNDGKKVAGGCGRYQPSYRRVGIDINAEWKKNVNEDTQERKIMLTAERVLEVFQQITDEDILVIGMDPQFARPEWMICTVLPVPPLAVRPAVVTFGSAKNQDDLTHKLSDIIKTNQQLQRNEANGAAAHVLTDDVRLLQFHVATLVDNCIPGLPTATQKGGRPLKSIKQRLKGKEGRIRGNLMGKRVDFSARTVITADPNLPIDTVGVPRTIAQNLTFPEIVTPFNVDKLQELVNRGDTQYPGAKYIIRENGARVDLRYHPRAADLHLQPGYRVERHMKDGDIIVFNRQPTLHKMSMMGHRVKILPWSTFRMNLSVTSPYNADFDGDEMNLHLPQSLETRAEIEEIAMVPRQLITPQANKPVMGIVQDTLCAVRMMTKRDVFIDWPFMMDLLMYLPTWDGKVPQPAILKPKPLWTGKQVFSLIIPGNVNVLRTHSTHPDSEDSGPYKWISPGDTKVIIEHGELLSGIVCSKTVGKSAGNLLHVVTLELGYEIAANFYSHIQTVINAWLIREGHTIGIGDTIADQATYLDIQNTIRKAKQDVVDVIEKAHNDDLEPTPGNTLRQTFENKVNQILNDARDRTGSSAQKSLSEFNNFKSMVVSGSKGSKINISQVIACVGQQNVEGKRIPFGFRHRTLPHFIKDDYGPESKGFVENSYLAGLTPSEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKAMESVMVNYDGTVRNSLAQMVQLRYGEDGLDGMWVENQNMPTMKPNNAVFERDFRMDLTDNKFLRKNYSEDVVREIQESEDGISLVESEWSQLEEDRRLLRKIFPRGDAKIVLPCNLQRLIWNAQKIFKVDLRKPVNLSPLHVISGVRELSKKLIIVSGNDEISKQAQYNATLLMNILLRSTLCTKNMCTKSKLNSEAFDWLLGEIESRFQQAIAQPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKEIINVSKTLKTPSLTVFLTGAAAKDPEKAKDVLCKLEHTTLKKVTCNTAIYYDPDPKNTVIAEDEEWVSIFYEMPDHDLSRTSPWLLRIELDRKRMVDKKLTMEMIADRIHGGFGNDVHTIYTDDNAEKLVFRLRIAGEDKGEAQEEQVDKMEDDVFLRCIEANMLSDLTLQGIPAISKVYMNQPNTDDKKRIIITPEGGFKSVADWILETDGTALLRVLSERQIDPVRTTSNDICEIFEVLGIEAVRKAIEREMDNVISFDGSYVNYRHLALLCDVMTAKGHLMAITRHGINRQEVGALMRCSFEETVDILMEAAVHAEEDPVKGVSENIMLGQLARCGTGCFDLVLDVEKCKYGMEIPQNVVMGGGFYGSFAGSPSNREFSPAHSPWNSGVTPTYAGAAWSPTTGGMSPGAGFSPAGNTDGGASPFNEGGWSPASPGDPLGALSPRTPSYGGMSPGVYSPSSPQFSMTSPHYSPTSPSYSPTSPAAGQSPVSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPSSPSYSPSSPSYSPSSPRYSPTSPTYSPTSPTYSPTSPTYSPTSPTYSPTSPSYESGGGYSPSSPKYSPSSPTYSPTSPSYSPTSPQYSPTSPQYSPSSPTYTPSSPTYNPTSPRGFSSPQYSPTSPTYSPTSPSYTPSSPQYSPTSPTYTPSPSEQPGTSAQYSPTSPTYSPSSPTYSPASPSYSPSSPTYDPNS 379BB183B957D2D5 med7_caeel Mediator of RNA polymerase II transcription subunit 7 let-49 mdt-7 med-7 Y54E5B.3 Mediator complex subunit 7 CeMED7 Lethal protein 49 protein protein caeel Caenorhabditis elegans MLPGFGAQTVSPFPNPPEYASAYTSDRINNGSAPPPPHPLTEFKVYGEEYRLEDDVIAPLKNAGVAELYKNKNNWKTEMKKLNRSAIVAFFDLVEILIRAPDHPMREEKMVDLHTIFINMHHLINEFRPVQARDSVRILQERQIEELSDICKDFKKYLRDGREVVDDQFQMIRGKLPAPPQPSELTRVKLQDGVLHMLQETEASDDVEMKEEEGSEYSKKKARLELLSREDGPPSVVHLLARQFHDISLKK 22E28653EB687721 med6_caeel Mediator of RNA polymerase II transcription subunit 6 mdt-6 med-6 Y57E12AL.5 Mediator complex subunit 6 CeMED6 c-MED6 protein protein caeel Caenorhabditis elegans MMPRMGPPAAARQDNPLHVSFRNPQWPPNFINKDNVLDYFCNQANAFYEMNSCNQQIRMQNIVNRTVEECLRTMPGIQYVLWYSQPPLFIICKQRRNNVTNVSPIAYYYVINGSVHQAPDMYSLVQSRLLGALEPLRNAFGEVTNYSRYNTAKGYYWEFKNKPNVKKREEEKKEDEEEKLEDRSTNFQKTRTMMLLNQLFSEMPAEDALEREEKEEVEEEEEETLKTEEPTTSTDEPKFAEPTARTTSKQ 4AFE17BA9BDE8511 med21-med7-1 2 5 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association Fig 2A med21-med7-2 3 7 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component physical association physical association Fig 2B med21-med7-3 1 8 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey association association Fig 2C med6-rpb1-2 1 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Fig 2C med6-rpb1-1 3 9 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component physical association physical association Fig 2B med6-rpb1-3 4 5 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component physical association physical association Fig 2D