UniProt UniProt http://www.uniprot.org Adaptor complex-independent clathrin function in yeast. Adaptor complex-independent clathrin function in yeast. Mol. Biol. Cell 10 (3643-3659) 1999 imex curation Yeung BG., Phan HL., Payne GS. gpayne@mednet.ucla.edu 16-April-2008: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yeung BG., Phan HL., Payne GS. gpayne@mednet.ucla.edu Accepted 2008-MAR-22 by CDEROW. Mol. Biol. Cell 10 (3643-3659) 1999 Only protein-protein interactions imex curation Adaptor complex-independent clathrin function in yeast. Adaptor complex-independent clathrin function in yeast. Mol. Biol. Cell 10 (3643-3659) 1999 imex curation Yeung BG., Phan HL., Payne GS. gpayne@mednet.ucla.edu 16-April-2008: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Yeung BG., Phan HL., Payne GS. gpayne@mednet.ucla.edu Accepted 2008-MAR-22 by CDEROW. 16-April-2008: Contacted by JYOTI. Mol. Biol. Cell 10 (3643-3659) 1999 Only protein-protein interactions imex curation Adaptor complex-independent clathrin function in yeast. Adaptor complex-independent clathrin function in yeast. Mol. Biol. Cell 10 (3643-3659) 1999 imex curation Yeung BG., Phan HL., Payne GS. gpayne@mednet.ucla.edu 16-April-2008: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yeung BG., Phan HL., Payne GS. gpayne@mednet.ucla.edu Accepted 2008-MAR-22 by CDEROW. 16-April-2008: Contacted by JYOTI. Mol. Biol. Cell 10 (3643-3659) 1999 Only protein-protein interactions imex curation Adaptor complex-independent clathrin function in yeast. Adaptor complex-independent clathrin function in yeast. Mol. Biol. Cell 10 (3643-3659) 1999 imex curation Yeung BG., Phan HL., Payne GS. gpayne@mednet.ucla.edu 16-April-2008: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast chromatography chromatography technology column chromatography chromatography western blot western blot Immuno blot Immuno blot Yeung BG., Phan HL., Payne GS. gpayne@mednet.ucla.edu Accepted 2008-MAR-22 by CDEROW. 16-April-2008: Contacted by JYOTI. Mol. Biol. Cell 10 (3643-3659) 1999 Only protein-protein interactions imex curation Adaptor complex-independent clathrin function in yeast. Adaptor complex-independent clathrin function in yeast. Mol. Biol. Cell 10 (3643-3659) 1999 imex curation Yeung BG., Phan HL., Payne GS. gpayne@mednet.ucla.edu 16-April-2008: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Yeung BG., Phan HL., Payne GS. gpayne@mednet.ucla.edu Accepted 2008-MAR-22 by CDEROW. 16-April-2008: Contacted by JYOTI. Mol. Biol. Cell 10 (3643-3659) 1999 Only protein-protein interactions imex curation ap1s1_yeast AP-1 complex subunit sigma-1 APS1 YAP19 YLR170C L9470.16 Sigma1-adaptin Golgi adaptor AP-1 19 kDa adaptin HA1 19 kDa subunit Clathrin coat assembly protein AP19 Clathrin coat-associated protein AP19 Clathrin assembly protein complex 1 sigma-1 small chain protein protein yeast Saccharomyces cerevisiae Baker's yeast MTQLKYLLLVSRQGKIRLKKWYTAMSAGEKAKIVKDLTPTILARKPKMCNIIEYNDHKVVYKRYASLYFIVGMTPDVDNELLTLEIIHRFVETMDTYFGNVCELDIIFNFSKVYDILNEMIMCDGSIAESSRKEVLHHVTVMDTMESNDNLERVLS 1A584883F1DF815D 1A584883F1DF815D apm2_yeast Adaptin medium chain homolog APM2 APM2 Adaptin-mu1-II YHL019C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSSLFILDENLEPLVSKNIRALPNLSSVLSSFKQCYHDGSPPILSQNDWFFIHLKRDFLHFVSVIHTTDKPNIDLMTILAFLEQFYHLLQKYFEIEVLTKNVILDNILLVLELIDECIDFGIVQVTDPSIIKDYIRVKVNVPRVTVDNEEWSPGEESSSSSGSDSDSEYSNTNKRKDKKKKRKKKKGTKGKSVGKSKLKSIMVNNKENRGINVVETVKETLRNKNDTGKEAANDELPNDGNDLYINGDIAKTIIMPISWRTKGIHYAKNEFFLDVIERVQYLMDFEKGVIRKNLIHGEIVCRCYLSGMPKLKISINKILNRDPQFMSNSSFHQCVSLDSINTIEKDEEKNSDDDAGLQAATDAREIEFIPPDGEFVLCQYELKRHVKDAPMVRLKDFEIKPKLKKFKIQIVTKIQTNFKPTNSTSKLNVRIPLTKVFQEYKIDLSKQIRFKANIGKVVFNLSDDFLLWEIQTMKGHREHSTNKSSQYNSDEDDPNTCASMVAEFPLFNQEEYDRLQEEMKTSMNPPPLRTGPRLEELYRQVHDQQTSHVTPRDKLVNIDFEIPYCTCSGLKVEYLKVEEPQLQYQSFPWVRYKTVSDEEYAYIV 7E216B11325EEE3C 7E216B11325EEE3C ap1b1_yeast AP-1 complex subunit beta-1 APL2 Beta-1-adaptin Clathrin assembly protein complex 1 beta-1 large chain Clathrin assembly protein large beta-1 chain YKL135C protein protein yeast Saccharomyces cerevisiae Baker's yeast MPPLDKRIKKFLKDSIRIAPKISGKGELSELRTGLVSQYPQTRKDAIKKTIQQMTLGKDVSSLFPDVLKNIATIDVEQKKLVYLYVMNYAETHPELCILAVNTFITDAQDPNPLIRCMAIRTMSMIRVDKILEYIETPLRRTLHDDNAYVRKTAVICVAKLFQLNKDLCVELGVVEDLVNALDDSNPLVIANATAALIEIHNMDMDAVDLSSLIQSHVSQFLLALNECTEWARIIILGTLSEYSAKDSLEAQDIIDRVTAHLQHVNPAVVLATIKVIVRNLPQIEYSSNSLIMKRLSSAFVSLMSTPPEMQYVALKNIRIILEKYPELLTKELRIFYVKFNDPLYVKLEKIDILVRLVDPSNLKQCTLLLTELKEYAMEYEPEFVSRAIQALSQLGIKYAQESFVSKVLDILLELLERQDTIKDDCCISLCDLLRHCPGNDKMAKQVCAVFNTWSNPEVLLQSDIAKCNYVWLLGQHPNNFSDLESKINIFIENFVQEEALTQMSLLMTIVRLHATLTGSMLQSVLELATQQTHELDVRDMAMMYWRCLSMPNNESLVNDLCQNKLPMISNTLEKFSPEVLEKLLMELGTISSIYFKPDSNRRKGKKYVQNIVKGKHIEELESMAKNEISSKANDDVLLDFDERDDVTNTNAGMLNTLTTLGDLDDLFDFGPSEDATQINTNDTKAVQGLKELKLGGDSNGISSGGKNNPDVSGGNIVSQDLLDLF 89893945D4EC6C4A 89893945D4EC6C4A ap2s_yeast AP-2 complex subunit sigma Sigma2-adaptin Adaptin small chain Plasma membrane adaptor AP-2 17 kDa protein Clathrin coat assembly protein AP17 Clathrin coat-associated protein AP17 APS2 YAP17 YJR058C J1720 Clathrin assembly protein 2 sigma small chain protein protein yeast Saccharomyces cerevisiae Baker's yeast MAVQFILCFNKQGVVRLVRWFDVHSSDPQRSQDAIAQIYRLISSRDHKHQSNFVEFSDSTKLIYRRYAGLYFVMGVDLLDDEPIYLCHIHLFVEVLDAFFGNVCELDIVFNFYKVYMIMDEMFIGGEIQEISKDMLLERLSILDRLD DFFD1A567532AAD8 DFFD1A567532AAD8 ap1g1_yeast AP-1 complex subunit gamma-1 APL4 Clathrin assembly protein complex 1 gamma large chain Clathrin assembly protein large gamma chain Gamma-adaptin YPR029C protein protein yeast Saccharomyces cerevisiae Baker's yeast MGSSLRSFIKDVRGAKTLADERAIITKQSAKIRTKLRDDHLPHEKRRVNIQKLLYLYILGEKTHFGQVESINLIASDDFVDKRLGYLAATLLLDESEDLLTLLTNMLNNDLHHPNKYAVSLALTSLGFLSSPELARDLYPDVENIIKNSRDPFLLKKALQCAAKLIFKDVSLLEIFNIEDITKILSSHSICTHGVLLGVTKIIQSILLIGLNRKKDEDEDEDGIDYSNDILSPLSLLLRDFFIRLENMNSKNIEPGYDVQGICDPFLQCEIIYTLKLYFQVGELLNSNNVLDYKDNFCDLLTRIATNTDSTKNSGQAILYETVKTIFSLDLNQPLRVLGINILAKFLAGKDNNTKYVSLNTLLKVVPQEPTAVQRHRKFISHCLQDTDVSIRMRALELSFAILDDSNLVELVNELMKFLAKQDEDSKDLIIYTIDHLIDTFDTRVVKDESWKLDVFFNILKLVGSFINYEKINDILIIINNTSQLSDKSEFLRKMLTISLNGTSAEISEENIGWQLVLIWCIGEYGDLVLNEGNKNGADIINESSITDYLLTLQELYTATNLKIINYILTAALKLSVRFHDAKNIEKLRQLILSYTDSTDLSLQMKSNQYEIFFNQSISVKKIILETMPKFEKITEEQDNGKALSKNLISNEPVDLLSDLLGEDSKAESKASTGDNVKPIDILEEIFGEKNDIAQVPKNANKEESINHSSAVEANSGVTLPLDANKIYDSSSLNVYASLLSANSGLAHLDLYFQAKSLISDLKTFCAVPKAQKLTLGQLYPSSTINASQICKQSLKISGSGKLKLRVKLDFHLNGSSSITNEQFDHKFDETL ABB619E9DC4F565C ABB619E9DC4F565C ap2m_yeast AP-2 complex subunit mu APM4 YOL062C O1210 Mu2-adaptin Plasma membrane adaptor AP-2 50 kDa protein Adaptin medium chain APM4 Clathrin coat assembly protein AP50 Clathrin coat-associated protein AP50 Clathrin assembly protein complex 2 mu medium chain AMP1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MISGVLVYSSRGELVLNKFFKNSLKRSISDIFRVQVINNLDVRSPVLTLGSTTFHHIRSRHGDNLWLVTITRSNANSAAIWEFLYKLDAVMNAYRLDREEALKEEFMIVHEMLDIMLGGNGIPIDTELNSVIAQMSVKPVRNMGGLLDSPDGNDVLSSSSSPTSSAGELHFPKFLTKRSSSFLGQGDSTSDFYDNNKITWRPKGIIHKKDEVFLYVNERINILVSRDGSILKSYVDGTIDITTHLSGTPICRFGLNDSLGMQSEDEKKWLAQQQRHSGSDFGNKNFIPKAAAGSVLLEDCKFHECVSLDKFNRNHIIEFVPPDGSMELMKYHVRDNINLPFKVTPIVTHSTRDNEIDYRITLKSLFPGKLSAKDVVLHIPVPPSTVDCKISVSNGHCKFVPEENAMIWRFNKYNGLTENTLSAVTVSTSDTTQLNLQQWTRPPISLEFEVMMFSNSGLVVRYFTISGKDSKHRAVKWIKYISKAGSYEVRY DCAF8F6BF8D43D45 DCAF8F6BF8D43D45 ap2b_yeast AP-2 complex subunit beta APL1 YAP80 YJR005W J1422 Clathrin assembly protein complex 2 beta large chain Clathrin assembly protein large beta chain Beta-2-adaptin Beta-adaptin protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDQKVFARYKANEIVTDLQHFGVKKFKSNITRRKNALRKIIANLVLGNYGEMSVLFSELLKFWQIEDDLEVKRICHEYIRVIGALKPQQAREALPFIMDDFKSRDEKLQIMALRTLVLVPVKELSDQAFDCIISLVNHKSPPEQVTRTAIYALLDLDEIDHERVLGLSSILHDIVKAQSSSPEVIVAALHTLYSIHEKNANMEPFRIPLELAFDMLELLPELNEWNKATVLEVLTTSVVPQHYLDTHEMIELALPYLQQVNTYVVLNSLKFIMYLLNYVDVIKETLAEKLSNSVIALLDKPPELQFLVLRNVILLLLSRESSLLRLDISYFFIEYNDPIYIKDTKLECLYLLANKETLPRILEELEQYATDIDIQMSRKSVRAIGNLAVKLDEDSVHDCVAVLLDLLEFGVDYVVQEIISVFRNILRKYPNNFKANVTELVKHTEVVQEPESKNAMIWIITQYSDVIPNYLELFRVFSSNMFSETLEVQFSILNSAIKFFIRSPTKETEELCMDLLKGCIDHENNPDLRDKTLMYWRLLSLTKTSRISNAITFESLKSVLDGELPLIEMNTKLDPTVLEELELNIGTIVSIYLKPVSHIFRLNKTKLLPQSPILNPNKDLLPVVGNSFPPTGANRDRQNSESQSSTKSRKTAMMDDYDKPAEKINQLKGKRKSSSNNPSKLSRKPSTLLRKLSMKRPFS 556A8F3EF8B3930F 556A8F3EF8B3930F clh_yeast Clathrin heavy chain CHC1 YGL206C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDLPIEFTELVDLMSLGISPQFLDFRSTTFESDHFVTVRETKDGTNSVAIVDLAKGNEVTRKNMGGDSAIMHPSQMVISVRANGTIVQIFNLETKSKLKSFTLDEPVIFWRWLSETTLGFVTARSILTSNVFDGNVNAKPQLLTLRHANLNNTQIINFVANKNLDWFAVVGILQENGRIAGRIQLFSKQRNISQAIDGHVAIFTNILLEGNGSTPVQVFVTGNRNATTGAGELRIIEIDHDASLPSQYQKETTDIFFPPDATNDFPIAVQVSEKYGIIYLLTKYGFIHLYELETGTNLFVNRITAESVFTAAPYNHENGIACINKKGQVLAVEISTSQIVPYILNKLSNVALALIVATRGGLPGADDLFQKQFESLLLQNDYQNAAKVAASSTSLRNQNTINRLKNIQAPPGAISPILLYFSTLLDKGKLNKEETIELARPVLQQDRKQLFEKWLKEDKLECSEELGDIVKPFDTTLALACYLRAGAHAKVISCLAELQQFEKIIPYCQKVGYQPNFLVLISSLIRSSPDRASEFAVSLLQNPETASQIDIEKIADLFFSQNHIQQGTSLLLDALKGDTPDQGHLQTRVLEVNLLHAPQVADAILGNNIFSHYDKPTIASLSEKAGLYQRALENYTDIKDIKRCVVHTNALPIDWLVGYFGKLNVEQSLACLKALMDNNIQANIQTVVQVATKFSDLIGPSTLIKLFEDYNATEGLYYYLASLVNLTEDKDVVYKYIEAAAKMKQYREIERIVKDNNVYDPERVKNFLKDANLEDQLPLVIVCDRFDFVHEMILYLYKSQNLKFIETYVQQVNPSKTAQVVGALLDMDCDEAFIQSLLQSVLGQVPINELTTEVEKRNRLKILLPFLEQSLSQGIQDQAVYNALAKIYIDSNNSPEKFLKENDQYDTLDVGHYCEKRDPYLAYIAYEKGQNDDDLIRITNENSMYKYQARYLLERSDLDLWNKVLNQENIHRRQLIDSVISVGIPELTDPEPVSLTVQAFMTNGLKLELIELLEKIILEPSPFNENVALQGLLLLSAIKYEPTKVSSYIEKLDNYDADEIAPLCIEHDLKEEAFEIYDKHEMYGKALKVLIEDIMSLDRAASYADKINTPELWSQIGTAQLDGLRIPDAIESYIKAEDPSNYENVIDIAEQAGKYEELIPFLLMARKTLKEPKIDGALILAYAELNKIHEIENLLAGSNVANLDHVGDKLFENKEYKAARLCYSAVSNYSKLASTLVYLGDYQAAVDTARKASNIKVWKLVNDACIEKKEFKLAQICGLNLIVHAEELDELVERYESNGYFEELISLFEAGLGLERAHMGMFTELAILYSKYEPDKTFEHLKLFWSRINIPKVIRAVEQAHLWSELVFLYAHYDEWDNAALTLIEKSTKDLDHAYFKEVVVKVSNLEIYYKAINFYVKFHPSLLVDLLTSLTPRLDIPRTVKIFSKSDNLPLIKPFLINVLPKNNSVVNQAYHDLMIEEEDYKALQDAVDSYDKFDQLGLASRLESHKLIFFKKIGALLYRRNKKWAKSLSILKEEKLWKDAIETAAISQDPKVVEALLTYFVETGNREGFVALLYAAYNLVRIEFVLEISWMNSLEDYIKPFEISIKKEQNDSIKKITEELAKKSGSNEEHKDGQPLMLMNSAMNVQPTGF 622F3083DF2FE315 622F3083DF2FE315 ap1m1_yeast AP-1 complex subunit mu-1-I APM1 YAP54 YPL259C P0394 Mu1-I-adaptin Mu(1)-adaptin Golgi adaptor AP-1 54 kDa protein HA1 54 kDa subunit Clathrin coat assembly protein AP54 Clathrin coat-associated protein AP54 Clathrin assembly protein complex 1 mu-1-I medium chain protein protein yeast Saccharomyces cerevisiae Baker's yeast MASAVYFCDHNGKPLLSRRYRDDIPLSAIDKFPILLSDLEEQSNLIPPCLNHNGLEYLFIQHNDLYVVAIVTSLSANAAAIFTFLHKLVEVLSDYLKTVEEESIRDNFVIIYELLDEVMDYGIPQITETKMLKQYITQKSFKLVKSAKKKRNATRPPVALTNSVSWRPEGITHKKNEAFLDIVESINMLMTQKGQVLRSEIIGDVKVNSKLSGMPDLKLGINDKGIFSKYLDDDTNIPSASATTSDNNTETDKKPSITSSSATNKKKVNIELEDLKFHQCVRLSKFENEKIITFIPPDGKFDLMNYRLSTTIKPLIWCDVNVQVHSNSRIEIHCKAKAQIKRKSTATNVEILIPVPDDADTPTFKYSHGSLKYVPEKSAILWKIRSFPGGKEYSMSAELGLPSISNNEDGNRTMPKSNAEILKGPVQIKFQIPYFTTSGIQVRYLKINEPKLQYKSYPWVRYITQSGDDYTIRLT C6B11153B845921C C6B11153B845921C kex2_yeast Kexin KEX2 QDS1 YNL238W N1122 Protease KEX2 Proteinase YSCF protein protein yeast Saccharomyces cerevisiae Baker's yeast MKVRKYITLCFWWAFSTSALVSSQQIPLKDHTSRQYFAVESNETLSRLEEMHPNWKYEHDVRGLPNHYVFSKELLKLGKRSSLEELQGDNNDHILSVHDLFPRNDLFKRLPVPAPPMDSSLLPVKEAEDKLSINDPLFERQWHLVNPSFPGSDINVLDLWYNNITGAGVVAAIVDDGLDYENEDLKDNFCAEGSWDFNDNTNLPKPRLSDDYHGTRCAGEIAAKKGNNFCGVGVGYNAKISGIRILSGDITTEDEAASLIYGLDVNDIYSCSWGPADDGRHLQGPSDLVKKALVKGVTEGRDSKGAIYVFASGNGGTRGDNCNYDGYTNSIYSITIGAIDHKDLHPPYSEGCSAVMAVTYSSGSGEYIHSSDINGRCSNSHGGTSAAAPLAAGVYTLLLEANPNLTWRDVQYLSILSAVGLEKNADGDWRDSAMGKKYSHRYGFGKIDAHKLIEMSKTWENVNAQTWFYLPTLYVSQSTNSTEETLESVITISEKSLQDANFKRIEHVTVTVDIDTEIRGTTTVDLISPAGIISNLGVVRPRDVSSEGFKDWTFMSVAHWGENGVGDWKIKVKTTENGHRIDFHSWRLKLFGESIDSSKTETFVFGNDKEEVEPAATESTVSQYSASSTSISISATSTSSISIGVETSAIPQTTTASTDPDSDPNTPKKLSSPRQAMHYFLTIFLIGATFLVLYFMFFMKSRRRIRRSRAETYEFDIIDTDSEYDSTLDNGTSGITEPEEVEDFDFDLSDEDHLASLSSSENGDAEHTIDSVLTNENPFSDPIKQKFPNDANAESASNKLQELQPDVPPSSGRS 5DCEEDADCF439C78 5DCEEDADCF439C78 apl2-apm2 1 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig: 1E apm1-apl2-3 1 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Fig: 1B & 1D apm1-aps1 1 6 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 1 F apl2-aps1 1 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Fig: 1F apm4-apl1 1 9 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Fig. 1D apl1-aps2 3 9 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association Fig. 1A & 1C This interaction requires the presence of Apl3. apl2-aps1-1 3 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Fig. 1C This interaction does not require the presence of Apl3 apl2-complex 3 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role bait bait association association Fig. 1A apl2-chc1 5 13 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 8 unspecified role unspecified role bait bait c-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecobb Escherichia coli (strain B / BL21) physical association physical association Fig. 2A A C-terminal construct of APL2 was used to show the interaction because clathrin-binding sites in mammalian orthologs are located toward the C-termini. apl2-chc1-1 2 8 unspecified role unspecified role bait bait c-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecobb Escherichia coli (strain B / BL21) 13 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association Fig. 2B GST-Apl2p (1) bound clathrin heavy chain A C-terminal construct of APL2 was used to show the interaction because clathrin-binding sites in mammalian orthologs are located toward the C-termini. apl2-chc1-2 1 13 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 2C chc1-kex2 4 15 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 5 & 9 The interaction is not affected by the multiple deletions of Apl2, Apl4, Apm1 and Aps1 or of Apl1, Apl2 and Apl6. The peaks of clathrin heavy chain and Kex2p from wild-type cells occurred in fraction 42