IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Identification of profilin and src homology 3 domains as binding partners for Drosophila enabled. Identification of profilin and src homology 3 domains as binding partners for Drosophila enabled. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 imex curation Ahern-Djamali SM., Bachmann C., Hua P., Reddy SK., Kastenmeier AS., Walter U., Hoffmann FM. shawn.ahern-djamali@wl.com 22-Jan-2008: Contacted by JYOTI. 13-Nov-2007: Contacted by JYOTI. Drosophila third-instar larval library orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Ahern-Djamali SM., Bachmann C., Hua P., Reddy SK., Kastenmeier AS., Walter U., Hoffmann FM. shawn.ahern-djamali@wl.com Accepted 2007-OCT-28 by ORCHARD. 22-Jan-2008: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 orchard Only protein-protein interactions imex curation Identification of profilin and src homology 3 domains as binding partners for Drosophila enabled. Identification of profilin and src homology 3 domains as binding partners for Drosophila enabled. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 imex curation Ahern-Djamali SM., Bachmann C., Hua P., Reddy SK., Kastenmeier AS., Walter U., Hoffmann FM. shawn.ahern-djamali@wl.com 22-Jan-2008: Contacted by JYOTI. 13-Nov-2007: Contacted by JYOTI. Drosophila third-instar larval library orchard Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Ahern-Djamali SM., Bachmann C., Hua P., Reddy SK., Kastenmeier AS., Walter U., Hoffmann FM. murine Abl has subtypes in SPTR which is not specified in publication. colocalisation studies not added since intrinsic proteins of Male Rat Kangaroo Kidney (PtK2) Cells could not be found. shawn.ahern-djamali@wl.com Accepted 2007-DEC-20 by ORCHARD. 22-Jan-2008: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 orchard Only protein-protein interactions imex curation Identification of profilin and src homology 3 domains as binding partners for Drosophila enabled. Identification of profilin and src homology 3 domains as binding partners for Drosophila enabled. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 imex curation Ahern-Djamali SM., Bachmann C., Hua P., Reddy SK., Kastenmeier AS., Walter U., Hoffmann FM. shawn.ahern-djamali@wl.com 22-Jan-2008: Contacted by JYOTI. 13-Nov-2007: Contacted by JYOTI. Drosophila third-instar larval library orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Ahern-Djamali SM., Bachmann C., Hua P., Reddy SK., Kastenmeier AS., Walter U., Hoffmann FM. shawn.ahern-djamali@wl.com Accepted 2007-OCT-28 by ORCHARD. Drosophila third-instar larval library Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 orchard Only protein-protein interactions imex curation Identification of profilin and src homology 3 domains as binding partners for Drosophila enabled. Identification of profilin and src homology 3 domains as binding partners for Drosophila enabled. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 imex curation Ahern-Djamali SM., Bachmann C., Hua P., Reddy SK., Kastenmeier AS., Walter U., Hoffmann FM. shawn.ahern-djamali@wl.com 22-Jan-2008: Contacted by JYOTI. 13-Nov-2007: Contacted by JYOTI. Drosophila third-instar larval library orchard Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Ahern-Djamali SM., Bachmann C., Hua P., Reddy SK., Kastenmeier AS., Walter U., Hoffmann FM. shawn.ahern-djamali@wl.com Accepted 2007-DEC-20 by ORCHARD. 22-Jan-2008: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 orchard Only protein-protein interactions imex curation grap_drome Growth factor receptor-bound protein 2 drk E(sev)2B Downstream of receptor kinase Grb2 Protein enhancer of sevenless 2B CG6033 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MEAIAKHDFSATADDELSFRKTQILKILNMEDDSNWYRAELDGKEGLIPSNYIEMKNHDWYYGRITRADAEKLLSNKHEGAFLIRISESSPGDFSLSVKCPDGVQHFKVLRDAQSKFFLWVVKFNSLNELVEYHRTASVSRSQDVKLRDMIPEEMLVQALYDFVPQESGELDFRRGDVITVTDRSDENWWNGEIGNRKGIFPATYVTPYHS A1D0614AF358F3C0 src_mouse Neuronal proto-oncogene tyrosine-protein kinase Src Src pp60c-src Proto-oncogene c-Src protein protein mouse Mus musculus Mouse MGSNKSKPKDASQRRRSLEPSENVHGAGGAFPASQTPSKPASADGHRGPSAAFVPPAAEPKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLSTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKGETGKYLRLPQLVDMSAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL DIP protein P05480 original sequence version: 163 DIP protein P05480 original sequence version: 99 DIP protein P05480 original sequence version: 128 DIP protein P05480 original sequence version: 136 DIP protein P05480 original sequence version: 176 22CB5C6BE7A061C0 ena_drome Protein enabled CG15112 ena enb protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAMKKLYAKTSFTSKKPSSAANSTPILAYHQQQHQQPGNGICEFQVVAPGHSGELMIRRSQSMHHKMSPPVGGLGSKSEYYSIEELQELDLLDYRHPMYHHYQQQELRQRYHEHEQLVLQLPKATSPKAGPIYEAPQRSQQQQDQMLYVPTAAQRDSSSSAAATSIASSSTLTSSPSPSSSSSLIFSTLRKCVSPSNPSVNPNQPSKTQPSKLGCSMSFSIRTTTATAATAAAANAATATLSTQQQQQQAQQQHKQHLYSNIHHYLIRQQQQKQHYTLQRRHNSVKDKFIGGITTIFAEQSIIGARASVMVYDDNQKKWVPSGSSSGLSKVQIYHHQQNNTFRVVGRKLQDHEVVINCSILKGLKYNQATATFHQWRDSKFVYGLNFSSQNDAENFARAMMHALEVLSGRVANNPGGPPTNGNGYEEDMGYRTMTSEDAAILRQNNSIGGHVTPSAQTPTSQTNQNNIPQSPPTPQGHHRTSSAPPAPQPQQQQQQQQAQQMGQPGSHYGPTGNGPTSNGLPQQVNSQIPPAPQQQPQQQQFQQQQQQQQYQQMVQAGYAPSQQYQQPHYVLSNSNPNLTVHQYPTQQAQQQPPQAPQPPLQNGGMYMVGHGHLPSSASANSVVYASQQQMLPQAHPQAPQAPTMPGPGYGGPPVPPPQQQAENPYGQVPMPPPMNPSQQQQPGQVPLNRMSSQGGPGGPPAPAPPPPPPSFGGAAGGGPPPPAPPQMFNGAPPPPAMGGGPPPAPPAPPAMGGGPPPAPGGPGAPPPPPPPPGLGGAPKKEDPQADLMGSLASQLQQIKLKKNKVTTSAPENSGSSTSSGGSGNYGTIGRSSNGMASMMDEMAKTLARRRAQAEKKDPDPEAEVKKRPWEKSNTLPHKLSGGAGSGSAGSGHEGANGNSGGAGSNTTNSGGESPRPMRKRFGSASEETILKVNGDGLSLALSNGDLDTLKAEIVREMRLEIQKVKNEIIDAIKSEFNRR 7B084A0AEC098297 abl_drome Tyrosine-protein kinase Abl Protein abelson Abl ABL-1 Dash CG4032 D-ash protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGAQQGKDRGAHSGGGGSGAPVSCIGLSSSPVASVSPHCISSSSGVSSAPLGGGSTLRGSRIKSSSSGVASGSGSGGGGGGSGSGLSQRSGGHKDARCNPTVGLNIFTEHNEALLQSRPLPHIPAGSTAASLLADAAELQQHQQDSGGLGLQGSSLGGGHSSTTSVFESAHRWTSKENLLAPGPEEDDPQLFVALYDFQAGGENQLSLKKGEQVRILSYNKSGEWCEAHSDSGNVGWVPSNYVTPLNSLEKHSWYHGPISRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRISEDPDGKVFVTQEAKFNTLAELVHHHSVPHEGHGLITPLLYPAPKQNKPTVFPLSPEPDEWEICRTDIMMKHKLGGGQYGEVYEAVWKRYGNTVAVKTLKEDTMALKDFLEEAAIMKEMKHPNLVQLIGVCTREPPFYIITEFMSHGNLLDFLRSAGRETLDAVALLYMATQIASGMSYLESRNYIHRDLAARNCLVGDNKLVKVADFGLARLMRDDTYTAHAGAKFPIKWTAPEGLAYNKFSTKSDVWAFGVLLWEIATYGMSPYPAIDLTDVYHKLDKGYRMERPPGCPPEVYDLMRQCWQWDATDRPTFKSIHHALEHMFQESSITEAVEKQLNANATSASSSAPSTSGVATGGGATTTTAASGCASSSSATASLSLTPQMVKKGLPGGQALTPNAHHNDPHQQQASTPMSETGSTSTKLSTFSSQGKGNVQMRRTTNKQGKQAPAPPKRTSLLSSSRDSTYREEDPANARCNFIDDLSTNGLARDINSLTQRYDSETDPAADPDTDATGDSLEQSLSQVIAAPVTNKMQHSLHSGGGGGGIGPRSSQQHSSFKRPTGTPVMGNRGLETRQSKRSQLHSQAPGPGPPSTQPHHGNNGVVTSAHPITVGALDVMNVKQVVNRYGTLPKGARIGAYLDSLEDSSEAAPALPATAPSLPPANGHATPPAARLNPKASPIPPQQMIRSNSSGGVTMQNNAAASLNKLQRHRTTTEGTMMTFSSFRAGGSSSSPKRSASGVASGVQPALANLEFPPPPLDLPPPPEEFEGGPPPPPPAPESAVQAIQQHLHAQLPNNGNISNGNGTNNNDSSHNDVSNIAPSVEEASSRFGVSLRKREPSTDSCSSLGSPPEDLKEKLITEIKAAGKDTAPASHLANGSGIAVVDPVSLLVTELAESMNLPKPPPQQQQKLTNGNSTGSGFKAQLKKVEPKKMSAPMPKAEPANTIIDFKAHLRRVDKEKEPATPAPAPATVAVANNANCNTTGTLNRKEDGSKKFSQAMQKTEIKIDVTNSNVEADAGAAGEGDLGKRRSTGSINSLKKLWEQQPPAPDYATSTILQQQPSVVNGGGTPNAQLSPKYGMKSGAINTVGTLPAKLGNKQPPAAPPPPPPNCTTSNSSTTSISTSSRDCTSRQQASSTIKTSHSTQLFTDDEEQSHTEGLGSGGQGSADMTQSLYEQKPQIQQKPAVPHKPTKLTIYATPIAKLTEPASSASSTQISRESILELVGLLEGSLKHPVNAIAGSQWLQLSDKLNILHNSCVIFAENGAMPPHSKFQFRELVTRVEAQSQHLRSAGSKNVQDNERLVAEVGQSLRQISNALNR 14287B02CC8FE86B prof_drome Profilin chic chi CG9553 Protein chickadee protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSWQDYVDNQLLASQCVTKACIAGHDGNIWAQSSGFEVTKEELSKLISGFDQQDGLTSNGVTLAGQRYIYLSGTDRVVRAKLGRSGVHCMKTTQAVIVSIYEDPVQPQQAASVVEKLGDYLITCGY 6E2942C12A81ADEA chic-ena 3 7 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey physical association physical association The region of the Ena protein used as bait in the yeast two-hybrid screen contains several matches to a putative profilin binding site (4, 9, 26, 27). To test whether these sequences were important for mediating the interaction with chickadee chic-ena-1 1 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey physical association physical association An interaction was detected when chickadee was cotransformed with Ena amino acids 440-490 and not Ena amino acids 490-684, suggesting that this interaction is mediated by proline-rich sequences in Ena 1 ena-chic 2 9 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 2 The Ena fusion protein precipitated chickadee from the fly lysate, unlike GST alone ena-abl 2 8 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh3 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 7 unspecified role unspecified role prey prey c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 28 26 physical association physical association 4 Ena bound specifically to the Drosophila and murine Abl-SH3 domains and the murine src SH3 domain. Ena also bound to the C-terminal but not to the N-terminal SH3 domain of Drk. ena-drk 2 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c terminal sh3 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 7 unspecified role unspecified role prey prey c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 34 32 physical association physical association 4 Ena bound specifically to the Drosophila and murine Abl-SH3 domains and the murine src SH3 domain. Ena also bound to the C-terminal but not to the N-terminal SH3 domain of Drk. ena-abl-1 4 7 unspecified role unspecified role prey prey Q8T4F7:p.[Pro763Ala;Pro770_Pro772delinsAlaAlaAla] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain PPP AAA Wed May 02 15:44:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro763ala,pro_pro_pro770-772ala_ala_ala' Tue Jun 30 18:42:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro763Ala;Pro770_Pro772delinsAlaAlaAla]' Q8T4F7:p.[Pro701Ala;Pro707_Pro709delinsAlaAlaAla] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain PPP AAA certain certain sequence position certain certain certain sequence position certain P A Wed May 02 19:41:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro701ala,pro_pro_pro707-709ala_ala_ala' Tue Jun 30 16:22:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro701Ala;Pro707_Pro709delinsAlaAlaAla]' drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 8 unspecified role unspecified role bait bait sh3 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 5 The optimal consensus binding motifs for Abl (Abl-Pro) and Src (Src-Pro) specifically blocked binding of Ena to the Abl or to the Src SH3 domains, respectively. Abl-Pro-AEPPPYPPPPIPGGK, Src-Pro-AERSSRPLPPIPGGK act as competitors for the interaction ena-src 2 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh3 domain sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 45 47 physical association physical association 4 Ena bound specifically to the Drosophila and murine Abl-SH3 domains and the murine src SH3 domain. Ena also bound to the C-terminal but not to the N-terminal SH3 domain of Drk. ena-src-1 4 7 unspecified role unspecified role prey prey Q8T4F7:p.[Pro701Ala;Pro707_Pro709delinsAlaAlaAla] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain PPP AAA Wed May 02 15:31:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro701ala,pro_pro_pro707-709ala_ala_ala' Tue Jun 30 18:47:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro701Ala;Pro707_Pro709delinsAlaAlaAla]' Q8T4F7:p.[Pro763Ala;Pro770_Pro772delinsAlaAlaAla] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain PPP AAA Wed May 02 16:30:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro763ala,pro_pro_pro770-772ala_ala_ala' Tue Jun 30 16:36:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro763Ala;Pro770_Pro772delinsAlaAlaAla]' drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh3 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 5 The optimal consensus binding motifs for Abl (Abl-Pro) and Src (Src-Pro) specifically blocked binding of Ena to the Abl or to the Src SH3 domains, respectively. Abl-Pro-AEPPPYPPPPIPGGK, Src-Pro-AERSSRPLPPIPGGK act as competitors for the interaction