IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. Nucleic Acids Res. (0305-1048) 1997 Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl 22-Jan-2008: Contacted by JYOTI. 13-Nov-2007: Contacted by JYOTI. 14.5 day old CD-1 mouse embryo cDNA library. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl Accepted 2007-DEC-29 by ORCHARD. 14.5 day old CD-1 mouse embryo cDNA library. Nucleic Acids Res. (0305-1048) 1997 orchard The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. Nucleic Acids Res. (0305-1048) 1997 Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl 22-Jan-2008: Contacted by JYOTI. 13-Nov-2007: Contacted by JYOTI. 14.5 day old CD-1 mouse embryo cDNA library. orchard human-xpcs2ba Homo sapiens xeroderma pigmentosum patient cell line with mutation in ERCC3 gene. xpcs2ba XPCS2BA patient cell line. This cell line was derived from patients with a single mutation in ERCC3 gene which causes complete inactivation of the NER function of the protein. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl Accepted 2007-DEC-29 by ORCHARD. 22-Jan-2008: Contacted by JYOTI. 14.5 day old CD-1 mouse embryo cDNA library. Nucleic Acids Res. (0305-1048) 1997 orchard The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. Nucleic Acids Res. (0305-1048) 1997 Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl 22-Jan-2008: Contacted by JYOTI. 13-Nov-2007: Contacted by JYOTI. 14.5 day old CD-1 mouse embryo cDNA library. orchard spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl Accepted 2007-DEC-29 by ORCHARD. 22-Jan-2008: Contacted by JYOTI. 14.5 day old CD-1 mouse embryo cDNA library. Nucleic Acids Res. (0305-1048) 1997 orchard The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. Nucleic Acids Res. (0305-1048) 1997 Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl 22-Jan-2008: Contacted by JYOTI. 13-Nov-2007: Contacted by JYOTI. 14.5 day old CD-1 mouse embryo cDNA library. orchard spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl Accepted 2007-DEC-29 by ORCHARD. 22-Jan-2008: Contacted by JYOTI. 14.5 day old CD-1 mouse embryo cDNA library. Nucleic Acids Res. (0305-1048) 1997 orchard The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. Nucleic Acids Res. (0305-1048) 1997 Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl 22-Jan-2008: Contacted by JYOTI. 13-Nov-2007: Contacted by JYOTI. 14.5 day old CD-1 mouse embryo cDNA library. orchard human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl Accepted 2007-DEC-29 by ORCHARD. 22-Jan-2008: Contacted by JYOTI. 14.5 day old CD-1 mouse embryo cDNA library. Nucleic Acids Res. (0305-1048) 1997 orchard The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. Nucleic Acids Res. (0305-1048) 1997 Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl 22-Jan-2008: Contacted by JYOTI. 13-Nov-2007: Contacted by JYOTI. 14.5 day old CD-1 mouse embryo cDNA library. orchard human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl Accepted 2007-DEC-29 by ORCHARD. 22-Jan-2008: Contacted by JYOTI. 14.5 day old CD-1 mouse embryo cDNA library. Nucleic Acids Res. (0305-1048) 1997 orchard The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. Nucleic Acids Res. (0305-1048) 1997 Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl 22-Jan-2008: Contacted by JYOTI. 13-Nov-2007: Contacted by JYOTI. 14.5 day old CD-1 mouse embryo cDNA library. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl Accepted 2007-DEC-29 by ORCHARD. 22-Jan-2008: Contacted by JYOTI. 14.5 day old CD-1 mouse embryo cDNA library. Nucleic Acids Res. (0305-1048) 1997 orchard The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. Nucleic Acids Res. (0305-1048) 1997 Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl 22-Jan-2008: Contacted by JYOTI. 13-Nov-2007: Contacted by JYOTI. 14.5 day old CD-1 mouse embryo cDNA library. orchard spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. pull down pull down western blot western blot Immuno blot Immuno blot Weeda G., Rossignol M., Fraser RA., Winkler GS., Vermeulen W., van 't Veer LJ., Ma L., Hoeijmakers JH., Egly JM. Hela WCE was subject to Heparin Ultrogel, DEAE, Sulfropropyl and Heparin-5PW. Fractions from heparin-5PW column were then immunoprecipitated. weeda@gen.fgg.eur.nl Accepted 2007-DEC-29 by ORCHARD. 22-Jan-2008: Contacted by JYOTI. 14.5 day old CD-1 mouse embryo cDNA library. Nucleic Acids Res. (0305-1048) 1997 orchard ercc3_human General transcription and DNA repair factor IIH helicase subunit XPB ERCC3 XPB XPBC Basic transcription factor 2 89 kDa subunit TFIIH basal transcription factor complex 89 kDa subunit DNA repair protein complementing XP-B cells Xeroderma pigmentosum group B-complementing protein DNA excision repair protein ERCC-3 protein protein human Homo sapiens Human MGKRDRADRDKKKSRKRHYEDEEDDEEDAPGNDPQEAVPSAAGKQVDESGTKVDEYGAKDYRLQMPLKDDHTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTSDITEYLRKLSKTGVPDGIMQFIKLCTVSYGKVKLVLKHNRYFVESCHPDVIQHLLQDPVIRECRLRNSEGEATELITETFTSKSAISKTAESSGGPSTSRVTDPQGKSDIPMDLFDFYEQMDKDEEEEEETQTVSFEVKQEMIEELQKRCIHLEYPLLAEYDFRNDSVNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPAKMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKIIVFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSHGGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEEDLAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSSQASRRFGTMSSMSGADDTVYMEYHSSRSKAPSKHVHPLFKRFRK DIP protein P19447 original sequence version: 86 DIP protein P19447 original sequence version: 127 DIP protein P19447 original sequence version: 126 DIP protein P19447 original sequence version: 191 DIP protein P19447 original sequence version: 182 F5F4D3A89A7DF826 prs8_human 26S proteasome regulatory subunit 8 PSMC5 SUG1 Proteasome 26S subunit ATPase 5 Proteasome subunit p45 p45/SUG Thyroid hormone receptor-interacting protein 1 26S proteasome AAA-ATPase subunit RPT6 protein protein human Homo sapiens Human MALDGPEQMELEEGKAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVGEVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKFIGEGARMVRELFVMAREHAPSIIFMDEIDSIGSSRLEGGSGGDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDSALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRGINLRKIAELMPGASGAEVKGVCTEAGMYALRERRVHVTQEDFEMAVAKVMQKDSEKNMSIKKLWK 29C6410C4A85A7F7 29C6410C4A85A7F7 tf2h1_human General transcription factor IIH subunit 1 General transcription factor IIH polypeptide 1 TFIIH basal transcription factor complex p62 subunit Basic transcription factor 2 62 kDa subunit GTF2H1 BTF2 protein protein human Homo sapiens Human MATSSEEVLLIVKKVRQKKQDGALYLMAERIAWAPEGKDRFTISHMYADIKCQKISPEGKAKIQLQLVLHAGDTTNFHFSNESTAVKERDAVKDLLQQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRLNVNATDSSSTSNHKQDVGISAAFLADVRPQTDGCNGLRYNLTSDIIESIFRTYPAVKMKYAENVPHNMTEKEFWTRFFQSHYFHRDRLNTGSKDLFAECAKIDEKGLKTMVSLGVKNPLLDLTALEDKPLDEGYGISSVPSASNSKSIKENSNAAIIKRFNHHSAMVLAAGLRKQEAQNEQTSEPSNMDGNSGDADCFQPAVKRAKLQESIEYEDLGKNNSVKTIALNLKKSDRYYHGPTPIQSLQYATSQDIINSFQSIRQEMEAYTPKLTQVLSSSAASSTITALSPGGALMQGGTQQAINQMVPNDIQSELKHLYVAVGELLRHFWSCFPVNTPFLEEKVVKMKSNLERFQVTKLCPFQEKIRRQYLSTNLVSHIEEMLQTAYNKLHTWQSRRLMKKT DIP protein P32780 original sequence version: 70 DIP protein P32780 original sequence version: 97 DIP protein P32780 original sequence version: 95 DIP protein P32780 original sequence version: 120 DIP protein P32780-1 original sequence version: 151 8F0FCEBBB1FC9C1D prs8_mouse 26S proteasome regulatory subunit 8 Psmc5 Sug1 Proteasome 26S subunit ATPase 5 Proteasome subunit p45 p45/SUG 26S proteasome AAA-ATPase subunit RPT6 protein protein mouse Mus musculus Mouse MALDGPEQMELEEGKAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVGEVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKFIGEGARMVRELFVMAREHAPSIIFMDEIDSIGSSRLEGGSGGDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDSALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRGINLRKIAELMPGASGAEVKGVCTEAGMYALRERRVHVTQEDFEMAVAKVMQKDSEKNMSIKKLWK DIP protein P62196 original sequence version: 79 29C6410C4A85A7F7 xpb-sug1 1 12 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association The wild-type human XPB (aa 31�742) was fused to the GAL4 DNA-binding domain (DBD) (aa 1�47) and used as bait in a two-hybrid screen for interacting proteins encoded by a library of cDNAs (mouse) The author describes an ERCCB3/XPB protein in an XP-B patient XP11BE with a splice mutation in the XPB gene leading to a frameshift altering the last 41 amino acids of the encoded protein. From sequence length, this would appear to be the construct the author is working with but there is no corresponding sequence in the UniProt database. xpb-sug1-1 7 12 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait c terminal 21 aa sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 19 21 physical association physical association The two-hybrid assay was used to assess the ability of mSUG1 to bind to the wild-type and XP11BE mutant XPB protein and the other core subunits of TFII 2A XP11BE mutant with a frame shift altering the last 41 amino acids of the encoded XPB protein was also found to interact weakly with mSUG1. It generated a transcriptional signal 5�10 times lower than the wild-type XPB fusion protein The author describes an ERCCB3/XPB protein in an XP-B patient XP11BE with a splice mutation in the XPB gene leading to a frameshift altering the last 41 amino acids of the encoded protein. From sequence length, this would appear to be the construct the author is working with but there is no corresponding sequence in the UniProt database. xpb-sug1-2 8 12 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 9 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association Crude extracts infected with either XPB alone or in combination with his-tagged mSUG1 baculoviruses were loaded onto a nickel-chelate affinity column. After washing and elution with 100 and 500 mM imidazole, immunoblot analysis indicated that XPB is retained onto the affinity column only in the presence of His-tagged mSUG1 3A The author describes an ERCCB3/XPB protein in an XP-B patient XP11BE with a splice mutation in the XPB gene leading to a frameshift altering the last 41 amino acids of the encoded protein. From sequence length, this would appear to be the construct the author is working with but there is no corresponding sequence in the UniProt database. xpb-sug1-3 3 9 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Even after extensive washing with buffer containing 500 mM KCl, mSUG1 still remained associated with XPB imidazole, immunoblot analysis indicated that XPB is retained onto the affinity column only in the presence of His-tagged mSUG1 3B The author describes an ERCCB3/XPB protein in an XP-B patient XP11BE with a splice mutation in the XPB gene leading to a frameshift altering the last 41 amino acids of the encoded protein. From sequence length, this would appear to be the construct the author is working with but there is no corresponding sequence in the UniProt database. xpb-sug1-4 4 12 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait physical association physical association Extract from insect cells co-infected with viruses expressing XPB and Hemagglutinin (HA)-tagged mSUG1 were immunoprecipitated with a MAb-XPB (1B3) or an anti MAb-SUG1 (2SU). Immunoprecipitates were analyzed on immunoblots with a MAb-HA (12CA5) (Fig. 4A) and MAb-XPB (1B3) (Fig. 4B). 4A The author describes an ERCCB3/XPB protein in an XP-B patient XP11BE with a splice mutation in the XPB gene leading to a frameshift altering the last 41 amino acids of the encoded protein. From sequence length, this would appear to be the construct the author is working with but there is no corresponding sequence in the UniProt database. xpb-sug1-5 4 9 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Extract from insect cells co-infected with viruses expressing XPB and Hemagglutinin (HA)-tagged mSUG1 were immunoprecipitated with a MAb-XPB (1B3) or an anti MAb-SUG1 (2SU). Immunoprecipitates were analyzed on immunoblots with a MAb-HA (12CA5) (Fig. 4A) and MAb-XPB (1B3) (Fig. 4B). 4B The author describes an ERCCB3/XPB protein in an XP-B patient XP11BE with a splice mutation in the XPB gene leading to a frameshift altering the last 41 amino acids of the encoded protein. From sequence length, this would appear to be the construct the author is working with but there is no corresponding sequence in the UniProt database. ercc3-psmc5 6 9 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component physical association physical association chromatography fractions from 0 0.9 M NH42SO4 gradient indicate that hSUG1 co-elutes with XPB in Heparin 5PW fractions 10 14. 5A ercc3-psmc5-1 5 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association immunoprecipitation of XPB from Heparin 5PW fraction 12 with a XPB monoclonal crosslinked to protein A�Sepharose specifically precipitates hSUG1. 5C ercc3-psmc5-2 5 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association Immunoprecipitations of hSUG1 from Heparin 5PW fraction 12 with the C-terminal-specific anti SUG1 MAb, 3SU or N-terminal-specific anti SUG1 MAb 2SU bound to protein G�Sepharose, specifically precipitate XPB 5B sug1-ercc3 2 11 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey association association 6 Co-purification of the hSUG1 protein with TFIIH by affinity chromatography. The author describes an ERCCB3/XPB protein in an XP-B patient XP11BE with a splice mutation in the XPB gene leading to a frameshift altering the last 41 amino acids of the encoded protein. From sequence length, this would appear to be the construct the author is working with but there is no corresponding sequence in the UniProt database.