IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Role for the PP2A/B56delta phosphatase in regulating 14-3-3 release from Cdc25 to control mitosis. Role for the PP2A/B56delta phosphatase in regulating 14-3-3 release from Cdc25 to control mitosis. Cell (0092-8674) 2006 imex curation Margolis SS., Perry JA., Forester CM., Nutt LK., Guo Y., Jardim MJ., Thomenius MJ., Freel CD., Darbandi R., Ahn JH., Arroyo JD., Wang XF., Shenolikar S., Nairn AC., Dunphy WG., Hahn WC., Virshup DM., Kornbluth S. No. This may be an in vitro only. Data lost in this paper because of the use of pan-Abs, I could not tell if Chk1 were human or Xenopus, Upstate do not sell PP2A A/C and in some cases I could not tell when they were using human or xenopus proteins Identity and further details on CDK2 taken from the manufacturers website. Cyclin E was stated to be human but it did not state which gene prodict was used. Identity of Xenopus CDC25 derived from alignment of sequence in S7 with sequences in UniProtKB. kornb001@mc.duke.edu 08-DEC-2006: Contacted by JYOTI. Only protein-protein interactions 3 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Margolis SS., Perry JA., Forester CM., Nutt LK., Guo Y., Jardim MJ., Thomenius MJ., Freel CD., Darbandi R., Ahn JH., Arroyo JD., Wang XF., Shenolikar S., Nairn AC., Dunphy WG., Hahn WC., Virshup DM., Kornbluth S. No. This may be an in vitro only. Data lost in this paper because of the use of pan-Abs, I could not tell if Chk1 were human or Xenopus, Upstate do not sell PP2A A/C and in some cases I could not tell when they were using human or xenopus proteins Identity and further details on CDK2 taken from the manufacturers website. Cyclin E was stated to be human but it did not state which gene prodict was used. Identity of Xenopus CDC25 derived from alignment of sequence in S7 with sequences in UniProtKB. kornb001@mc.duke.edu Accepted 2006-DEC-4 by JYOTI. 08-DEC-2006: Contacted by JYOTI. Cell (0092-8674) 2006 Only protein-protein interactions 3 imex curation Role for the PP2A/B56delta phosphatase in regulating 14-3-3 release from Cdc25 to control mitosis. Role for the PP2A/B56delta phosphatase in regulating 14-3-3 release from Cdc25 to control mitosis. Cell (0092-8674) 2006 imex curation Margolis SS., Perry JA., Forester CM., Nutt LK., Guo Y., Jardim MJ., Thomenius MJ., Freel CD., Darbandi R., Ahn JH., Arroyo JD., Wang XF., Shenolikar S., Nairn AC., Dunphy WG., Hahn WC., Virshup DM., Kornbluth S. No. This may be an in vitro only. Data lost in this paper because of the use of pan-Abs, I could not tell if Chk1 were human or Xenopus, Upstate do not sell PP2A A/C and in some cases I could not tell when they were using human or xenopus proteins Identity and further details on CDK2 taken from the manufacturers website. Cyclin E was stated to be human but it did not state which gene prodict was used. Identity of Xenopus CDC25 derived from alignment of sequence in S7 with sequences in UniProtKB. kornb001@mc.duke.edu 08-DEC-2006: Contacted by JYOTI. Only protein-protein interactions 3 xenla-oocyte Xenopus laevis oocyte Oocyte pull down pull down western blot western blot Immuno blot Immuno blot Margolis SS., Perry JA., Forester CM., Nutt LK., Guo Y., Jardim MJ., Thomenius MJ., Freel CD., Darbandi R., Ahn JH., Arroyo JD., Wang XF., Shenolikar S., Nairn AC., Dunphy WG., Hahn WC., Virshup DM., Kornbluth S. No. This may be an in vitro only. Data lost in this paper because of the use of pan-Abs, I could not tell if Chk1 were human or Xenopus, Upstate do not sell PP2A A/C and in some cases I could not tell when they were using human or xenopus proteins Identity and further details on CDK2 taken from the manufacturers website. Cyclin E was stated to be human but it did not state which gene prodict was used. Identity of Xenopus CDC25 derived from alignment of sequence in S7 with sequences in UniProtKB. kornb001@mc.duke.edu Accepted 2006-DEC-4 by JYOTI. 08-DEC-2006: Contacted by JYOTI. Cell (0092-8674) 2006 Only protein-protein interactions 3 imex curation Role for the PP2A/B56delta phosphatase in regulating 14-3-3 release from Cdc25 to control mitosis. Role for the PP2A/B56delta phosphatase in regulating 14-3-3 release from Cdc25 to control mitosis. Cell (0092-8674) 2006 imex curation Margolis SS., Perry JA., Forester CM., Nutt LK., Guo Y., Jardim MJ., Thomenius MJ., Freel CD., Darbandi R., Ahn JH., Arroyo JD., Wang XF., Shenolikar S., Nairn AC., Dunphy WG., Hahn WC., Virshup DM., Kornbluth S. No. This may be an in vitro only. Data lost in this paper because of the use of pan-Abs, I could not tell if Chk1 were human or Xenopus, Upstate do not sell PP2A A/C and in some cases I could not tell when they were using human or xenopus proteins Identity and further details on CDK2 taken from the manufacturers website. Cyclin E was stated to be human but it did not state which gene prodict was used. Identity of Xenopus CDC25 derived from alignment of sequence in S7 with sequences in UniProtKB. kornb001@mc.duke.edu 08-DEC-2006: Contacted by JYOTI. Only protein-protein interactions 3 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Margolis SS., Perry JA., Forester CM., Nutt LK., Guo Y., Jardim MJ., Thomenius MJ., Freel CD., Darbandi R., Ahn JH., Arroyo JD., Wang XF., Shenolikar S., Nairn AC., Dunphy WG., Hahn WC., Virshup DM., Kornbluth S. No. This may be an in vitro only. Data lost in this paper because of the use of pan-Abs, I could not tell if Chk1 were human or Xenopus, Upstate do not sell PP2A A/C and in some cases I could not tell when they were using human or xenopus proteins Identity and further details on CDK2 taken from the manufacturers website. Cyclin E was stated to be human but it did not state which gene prodict was used. Identity of Xenopus CDC25 derived from alignment of sequence in S7 with sequences in UniProtKB. kornb001@mc.duke.edu Accepted 2006-DEC-4 by JYOTI. 08-DEC-2006: Contacted by JYOTI. Cell (0092-8674) 2006 Only protein-protein interactions 3 imex curation q6p3p7_xentr Serine/threonine protein phosphatase 2A regulatory subunit ppp2r5c Ppp2r5d TEgg007l21.1-001 protein protein xentr Xenopus tropicalis (Western clawed frog) Silurana tropicalis MPNKNKKDKEPPKAGKSGKTGKEGQDNPEHEVSNKKSNNTPPPAQVSKIKVPTPQAVVKKEKRQSSSRFNVSNNRELQKLPALKDVPPAEQEKLFVQKLRQCCVLFDFVSDPLSDLKWKEVKRAALSEMVEYITHNRNVITEPIYPEVVHMFAVNMFRTLPPSSNPTGAEFDPEEDEPTLEAAWPHLQLVYEFFLRFLESPDFQPNVAKKYIDQKFVLQLLELFDSEDPRERDFLKTTLHRIYGKFLGLRAYIRKQINNIFYRFIYETEHHNGIAELLEILGSIINGFALPLKEEHKIFLLKVLLPLHKVKSLSVYHPQLAYCVVQFLEKDSTLTEPVVMALLKYWPKTHSPKEVMFLNELEEILDVIEPSEFVKVMEPLFRQLAKCVSSPHFQVAERALYYWNNEYIMSLISDNAAKILPIMFPSLYRNSKTHWNKTIHGLIYNALKLFMEMNQKLFDDCTQQFKAEKQKEKLKLKEREEAWIKIENLAKSNPQYPMYTDTSALNSPVAMETDGPLLEDVQMLKKTVKEEASQAQKDLKKERPLVRRKSELPQDIYTMKALESHCRADELISHDGH D43FF331944D7040 mpi1a_xenla M-phase inducer phosphatase 1-A cdc25-1-a cdc25a protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAESHIMSSEAPPKSNTGLNIRTNCRMILNLLREKDCSVTFSPEQPLTPVTDLAVGFSNLSTFSGETPKRCLDLSNLGDETAPLPTESPDRMSSGKLESPKTQFVQFDGLFTPDLAWKAKKCPKRNMNSVLPHLLCSTPSFKKASGGQRSLSNKENEGELFKNPNCKPVALLLPQEVVDSQLSPTPENKVDISLEEDCEMNILGSPISADPPCLDGAHDDIKMQNLDGFADFFSVDEEEMENPPGAVGNLSCSMAILLSGPLLNQDVEISNVNNISLNRSRLYRSPSMPEKLDRPMLKRPVRPLDSETPVRVKRRRSTSSPLQPEEENCQPQRRGTSLKKTLSLCDVDISTVLDEDCGHRQLIGDFSKVYALPTVTGRHQDLRYITGETLAALMHGDFNSLVEKFFIIDCRYPYEYDGGHIKSAFNLHRQEEVTDYFLQQPLTPLMVQKRLIIIFHCEFSSERGPKMCRFLREEDRASNDYPSLYYPELYLLKGGYKDFFPEYKELCEPQSYCPMHHQDFREDLLKFRTKCKTSVGDRKRREQVARLMKL 407B3B7AD8EC7216 cdk2_human Cyclin-dependent kinase 2 CDK2 p33 protein kinase Cell division protein kinase 2 CDKN2 protein protein human Homo sapiens Human MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL DIP protein P24941 original sequence version: 142 DIP protein P24941 original sequence version: 118 DIP protein P24941 original sequence version: 143 DIP protein P24941 original sequence version: 144 DIP protein P24941 original sequence version: 152 DIP protein P24941 original sequence version: 182 DIP protein P24941-1 original sequence version: 204 DIP protein P24941 original sequence version: 90 DIP protein P24941 original sequence version: 190 DIP protein P24941 original sequence version: 168 F90A0F4E70910B51 2a5d_human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform PPP2R5D PP2A B subunit isoform B'-delta PP2A B subunit isoform B56-delta PP2A B subunit isoform PR61-delta PP2A B subunit isoform R5-delta protein protein human Homo sapiens Human MPYKLKKEKEPPKVAKCTAKPSSSGKDGGGENTEEAQPQPQPQPQPQAQSQPPSSNKRPSNSTPPPTQLSKIKYSGGPQIVKKERRQSSSRFNLSKNRELQKLPALKDSPTQEREELFIQKLRQCCVLFDFVSDPLSDLKFKEVKRAGLNEMVEYITHSRDVVTEAIYPEAVTMFSVNLFRTLPPSSNPTGAEFDPEEDEPTLEAAWPHLQLVYEFFLRFLESPDFQPNIAKKYIDQKFVLALLDLFDSEDPRERDFLKTILHRIYGKFLGLRAYIRRQINHIFYRFIYETEHHNGIAELLEILGSIINGFALPLKEEHKMFLIRVLLPLHKVKSLSVYHPQLAYCVVQFLEKESSLTEPVIVGLLKFWPKTHSPKEVMFLNELEEILDVIEPSEFSKVMEPLFRQLAKCVSSPHFQVAERALYYWNNEYIMSLISDNAARVLPIMFPALYRNSKSHWNKTIHGLIYNALKLFMEMNQKLFDDCTQQYKAEKQKGRFRMKEREEMWQKIEELARLNPQYPMFRAPPPLPPVYSMETETPTAEDIQLLKRTVETEAVQMLKDIKKEKVLLRRKSELPQDVYTIKALEAHKRAEEFLTASQEAL DIP protein Q14738 original sequence version: 92 F15F71AF4E565387 mpip3_human M-phase inducer phosphatase 3 CDC25C Dual specificity phosphatase Cdc25C protein protein human Homo sapiens Human MSTELFSSTREEGSSGSGPSFRSNQRKMLNLLLERDTSFTVCPDVPRTPVGKFLGDSANLSILSGGTPKRCLDLSNLSSGEITATQLTTSADLDETGHLDSSGLQEVHLAGMNHDQHLMKCSPAQLLCSTPNGLDRGHRKRDAMCSSSANKENDNGNLVDSEMKYLGSPITTVPKLDKNPNLGEDQAEEISDELMEFSLKDQEAKVSRSGLYRSPSMPENLNRPRLKQVEKFKDNTIPDKVKKKYFSGQGKLRKGLCLKKTVSLCDITITQMLEEDSNQGHLIGDFSKVCALPTVSGKHQDLKYVNPETVAALLSGKFQGLIEKFYVIDCRYPYEYLGGHIQGALNLYSQEELFNFFLKKPIVPLDTQKRIIIVFHCEFSSERGPRMCRCLREEDRSLNQYPALYYPELYILKGGYRDFFPEYMELCEPQSYCPMHHQDHKTELLRCRSQSKVQEGERQLREQIALLVKDMSP DIP protein P30307 original sequence version: 148 DIP protein P30307 original sequence version: 88 DIP protein P30307 original sequence version: 112 DIP protein P30307 original sequence version: 108 0658A1F1B9B8996A cdc25a-cdk2-1 1 5 enzyme target enzyme target substrate substrate unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 enzyme enzyme unspecified role unspecified role c-terminal region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation Cyclin E present. Phosphorylation of Thr-138 1E-G we phosphorylated GST-Cdc25 with Cdk2 in vitro (Figure 1E), ppp2r5d-cdc25c-1 3 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3E Human Cdc25C also coprecipitated with HA-tagged B56 (δ isoform) from transfected HEK 293 cells (Figure 3E). ppp2r5d-cdc25c-2 2 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 3D, 3F (D) GST or GST-Cdc25 linked to glutathione-Sepharose was incubated in interphase extract, collected, and immunoblotted for B56 (top)