IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The BRCA1/BARD1 heterodimer modulates ran-dependent mitotic spindle assembly. The BRCA1/BARD1 heterodimer modulates ran-dependent mitotic spindle assembly. Cell (0092-8674) 2006 imex curation Joukov V., Groen AC., Prokhorova T., Gerson R., White E., Rodriguez A., Walter JC., Livingston DM. Xenopus interphase egg extracts were used in an immunodepletion experiment to indicate complete association of BRCA1 and BARD1. After depletion the supernatant was analysed for the lack of the two proteins. david_livingston@dfci.harvard.edu 01-DEC-2006: Contacted by JYOTI. XBARD1 and XBRCA1-specific Abs were used to deplete egg extracts of BRCA1 and BARD1. orchard Only protein-protein interactions 6 xenla-oocyte Xenopus laevis oocyte Oocyte anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Joukov V., Groen AC., Prokhorova T., Gerson R., White E., Rodriguez A., Walter JC., Livingston DM. Xenopus interphase egg extracts were used in an immunodepletion experiment to indicate complete association of BRCA1 and BARD1. After depletion the supernatant was analysed for the lack of the two proteins. david_livingston@dfci.harvard.edu Accepted 2006-NOV-21 by ORCHARD. 01-DEC-2006: Contacted by JYOTI. XBARD1 and XBRCA1-specific Abs were used to deplete egg extracts of BRCA1 and BARD1. Cell (0092-8674) 2006 orchard Only protein-protein interactions 6 imex curation The BRCA1/BARD1 heterodimer modulates ran-dependent mitotic spindle assembly. The BRCA1/BARD1 heterodimer modulates ran-dependent mitotic spindle assembly. Cell (0092-8674) 2006 imex curation Joukov V., Groen AC., Prokhorova T., Gerson R., White E., Rodriguez A., Walter JC., Livingston DM. Xenopus interphase egg extracts were used in an immunodepletion experiment to indicate complete association of BRCA1 and BARD1. After depletion the supernatant was analysed for the lack of the two proteins. david_livingston@dfci.harvard.edu 01-DEC-2006: Contacted by JYOTI. XBARD1 and XBRCA1-specific Abs were used to deplete egg extracts of BRCA1 and BARD1. orchard Only protein-protein interactions 6 xenla-oocyte Xenopus laevis oocyte Oocyte anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Joukov V., Groen AC., Prokhorova T., Gerson R., White E., Rodriguez A., Walter JC., Livingston DM. Co-Immunolocalisation studies wih tubulin cannot be entered since the type of tubulin used is not specified. CSF arrested Xenopus egg extracts were used in this experiment. david_livingston@dfci.harvard.edu Accepted 2006-NOV-21 by ORCHARD. 01-DEC-2006: Contacted by JYOTI. Cell (0092-8674) 2006 orchard Only protein-protein interactions 6 imex curation p70012_xenla numa1.S NuMA numa1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MTSHSGKMEALLCWVNSLKVDEPIERFSQLQDLSILLKVVGVLSGNSEETAPVLQQDLEQRLNCLCSFLQRYCQTGSSAQNLVQWQKILQGENLEVELSKVIVILFYYSTMSSKNPRKSEDIDHKTQTELASILRFVLDNEDALCLDDKLINFLKRKAPFPSSGNDPSSSSDAMTPNVSHRRKSEVRFLQLHSVASSSTVKSLSADAPSSPMIEVLHTPQFQMRRLKKQLSEVRECRDELEVELAENRKHLAEKEAQISLLQQQIENLRILRENQTELQEPKELEELREKNESLMIRLRDTLKQCQDMKADKKLLERKNDQLAEENGELSYKVRDLSNRLAQLQEALYETTEEQELSLSNWQQKQNQLESELSGAVGEKKYLEEHNLILQGKISMLEDQLKEMGEIDMPETGDCMGDILKLDDLKQELAVLNTQCLSLKEQIHQMEEEKSTAEVEMEAQKSRFESEKGQLQEIVTNLQTSLSEITFQKERLDNEARAQQEHLMCQITTLKLEISKLKSSLVHKDEELKGIHHKVEEERNEKNQLLENFKMLGGNLIGITQQLESKTKEVDYLREQQQKILCERDSTLSTLNEYKCKKDENSGVLNKTVKTLEQDHQTSLSVIEKLKSEKEELASKVQDLDAKMLGLIAKCQNLDSENDSQSKSHAATVESLKAQLSEQESQLKIYRKKVSSNELVSEENSKLKDQLLSVEESLRHLREHLEKEKTKFAASLDADLKRISHLEEEMKKLSESRDEALHNLDEERTAGKKIESQLKHLEEEYQKANESLQAKLAGSCAAIKQREEERDELSKVVDIWKAKYGESQQKIAQNSCHMQEQTEELKKTHSDVYQQLEGERSKVLMIEAKASETKSSQLEKINQLEGELSAANACIKEREAEEKKLVSALHSAEEKLKIAYQGESERLSHLETALSNAKQDLDCLAKELSDEKYKKAEFEAMVKVLKEQNSERIASLESELKNSLAVVKERKCESEKLSGEVEHLKRQLDDSSQKHKEALAQKNIEIKQLINAKEKATSDLAIKSEMGAQLQKAVDTHKSEFSALQNELSRSLDLLALKEGEVERLNKEAALRQEEIQQQQQTITKLTEEETALAALKDKVALQEKEIKQQVQATKGAEKEMAKLKSVISEKSKRIECLEQDIQNQKRDLSCIQEQHQSKLGESQGLQALIADLEKKCKEQKELICEAQNKAAEAKTLASEKASVSERQLEGIQALEIEIGKERQKACDLQKQLELSWAVQEEKETELQALKKELFHKVQELEQSQTSFTDSSGEALLYLSEAQERQQALTEAKEQAEQYQKEIEMKNKEVNSLQAEIKILSSKVTTNEEVSVDFEQRLLKETSKSAKLEEKMQKLHMELEASFKELLEKNCAIDCLTTEAQNLKGEADQQRMAVDSLQQKLSSKAETNHTLQQEIQAWQKNCAEKEQQICSLQQNLKSNQSLLEEFASLKHSYQEIIAERDLMQEKHQEELLSHKKLTERFQAELEKAKEDMTEIVLLKEKLHNQELQLHKFQSENSYSLTQISHLQQVNSQLLGANQSLSQISDQGAKKLESEMSTLKEQHKEEMKTLRLQYEKTLREGNKQVQETSLQLETVTSKYDHVKSKVLKDQKTFQEEKQRLLLQVQELNKQLSQQEKTIRSQQQKLKQREGETHEEADKSHKRVLELESQLEQQTQAVEHYKAQMEKAKVHYDAKKKQNQELSEELQSHIKQQEHLSKENADLKAESEQLHKELQHSLLQSKEVEQNCKNLSNRVRSLEASWSTLTGQLRDPGKFQLATAFHEESGHFCAPRQTRSHADVSIDSLDLSFGEDQLLNSTSERCNEEPATSSVHASSPDSLISGQLPKKVESLESLYFTPIPTRAQSKLESSIGSIGDLSLDSSKKTQSARRRTMQIINITMTKKTKEEPESANTSFYSLRSAPSYQSLHLQNPRRAGRPPAAISAPALASLPSQESLAKTEHFSSDDSLNNLPGYQHPTRRSARLSQTGGRSSFYMSTCQDEPDPQDDWTRIAELQARNKTCPPHLKTSYPLESRPSIFSSTITDEEVKLGDPKETLRRATLLPGQIQDSMTSTRRQTLAVPGAEHLKGHNISTRQQMKRVSEESHYGPDTPEAKKTATCFPRPMTPKDKHDTRRVSTMESKGSSSHQQAQPTRRQATAFSIFNTPKKLGNSLLKRGLNKKTTPKNSPRGRGANGSTSSTSNKPSHLSLRKSPSQRSPRVSTAKSPRASNKLFERKQQRNK 158AEF149543E5B5 tpx2a_xenla Targeting protein for Xklp2-A tpx2-a protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MEDTQDTYSYDAPSIFNFSSFHEDHNADSWFDQVTNAENIPPDQRRLSETSVNTEQNSKVEPVQTTPSKDDVSNSATHVCDVKSQSKRSSRRMSKKHRQKLLVKMKDTHLEKETAPPEYPPCKKLKGSSSKGRHAPVIKSQSTSSHHSMTSPKPKAQLTMPSTPTVLKRRNVLVKAKNSEEQELEKMQELQKEMLENLKKNEHSMKVAITGAGQPVKTFIPVTKPVDFHFKTDDRLKRTANQPEGDGYKAVDFASELRKHPPSPVQVTKGGHTVPKPFNLSKGKRKHEEASDYVSTAEQVIAFYKRTPARYHLRSRQREMEGPSPVKMIKTKLTNPKTPLLQTKGRHRPVTCKSAAELEAEELEMINQYKFKAQELDTRILEGGPVLLKKPLVKEPTKAIGFDLEIEKRIQQREKKEEIEEETFTFHSRPCPSKMLTDVVGVPLKKLLPVTVPQSPAFALKNRVRIPAQEEKQEEMVPVIKATRMPHYGVPFKPKLVEQRQVDVCPFSFCDRDKERQLQKEKRLDELRKDEVPKFKAQPLPQFDNIRLPEKKVKMPTQQEPFDLEIEKRGASKLQRWQQQIQEELKQQKEMVVFKARPNTVVHQEPFVPKKENRSLTESLSGSIVQEGFELATAKRAKERQEFDKCLAETEAQKSLLEEEIRKRREEEEKEEISQLRQELVHKAKPIRKYRAVEVKASDVPLTVPRSPNFSDRFKC F402B114123E839C q643y9_xenla RHAMM protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSFPKAPLKRFNEHIGCAPAPGSYDVKLYDGLKGPVSFEKSHRFQAKKDSNNESQIMDKEEELSPVRLRKHSFGSAQNLRQKPEKDADFVKEMKKQKSLEKEIRSLIKERTEQDKKLQALEEEFKKTEVKLVTALREKTSLSASIASMERHIADLDKANELLKTKFSDDSSKKKINSLCAELIEVKNKVDAKDKEIICKQANFEEQITVLQANLLSCKQTLETVQKKNTFLEENYQDANSHSEALEKDLDNARALIEELRGESRNLQDYLSGAQEQMQDMRMEMSTKEREFENKLKDISSSMSEQSKALAIQGEMESKLHETEKNLALALEKIAQLEQELNAVKEEKHKLIEEKAETEAKLCASMEQMGKISTAVSQCEQYKLCLDQTSDLLKQKEQEEMSTKENFSLREKELLAQIKELDEKYLAQIQEKETLASESREKERMLQIELEMVKEKMSKEEDSCHVLREKEKQLSMLLQKEMETCSFLREELQKVQEEMMKERHLLEEELEGTLDELDRLQIAEEHSDRFIKQLEEENKQRAGELDYLEETLKGKSAELERIKEVHRKAMLQLKEEQSNNTHKVQEYDSFRRSASTEIESLKSTNLSLLEKIAQAEKDKEMQEQLLEEQVRDKSVLEQQFIDLRAASSQTKEEYEQKLYELQETSNTTKEGLEQQLLDLHKTSAKAVEDLEEKLYKLQEASSKTKEVLEQQVKDLQEVSNKTRDGLEEQLQKVQEASTKTRESLEQELHELKETSANTNKGLEQQLHEIQKEASRTAEKLEQQLHEQQEVSAKTIKALEQQLKDLLETSTTTKQGLEQQLCKLQEDSTKTKNDLEQQLRDLQEASAKTQDGLEKQLHLLQEESAKTSKLLLQQLCDLQEVSNKTRENLEQQLQEMQETSSKTKAELERQLQELQETSSNTKDLEQQLQDACTKRIEFEQQQHELEELRLQLLALQESTALSKEKYISMICDAELNLEMKDAKLKELEEQQQLREDPLRMELEQLQKQSEQLHELKRKQEEELELLRKQSAKDEQDATEANKWRTLYEELQNKVRPFKQQLDAFEAEKNAMLNEHGAAQDELNKLSEAYAKLLGHQNQKQKIKHVMKLKTENSELKQEVSKLRAQLAKEKQVEKQLQAHLNEIEGVKRFDPSKAFKHDVKENVFPKAPLREGNRNKC 8BA0E5C463F4F75C q90x96_xenla RING-type E3 ubiquitin transferase BRCA1 brca1.L BRCA1 brca1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MTCSRMDIEGICSVISVMQKNLECPICLELMKEPVATKCDHIFCKFCMLQLLSKKKKGTVPCPLCKTEVTRRSLQESHRFKLLVEGQLKIIKAFEFDSGYKFFPSQEHTKGLDSTIEDVLVKEDQSIVHCKGYRNRKKGVFNRKTYEETGMLSVSKAEEQFAKEVTRLIPCRQKKPKKEAALIFSNCVPDSSDGDLLNKENGLRNDCSPLHYEKEDTQIPEMEEMVESDLAECEFAESAGSNLLGFDGPEGIPEISAETSINAAGNCDFYGRKTEQFPNDHHCSFKQNIADAEQNKRNQHCGNVPFAPMGKSNLDEKETVETDFDNQHNDSNPENNDPLGKVTKLMRRSTERVNEWLLKTNQDFSTLSAEEDPILDALALQNKETSDKRSCSSDDSELMPVLHKHAEKGISGGGFDKPAVGVKDKIFCKVYKRERKAMPPNNITCVAEVHHDSALETGKENMTLEYGTGMSHLMSKRKMVYSLNPENTSKKNDLANINGSINVFPDCISDANLELEDKSEADSNSADACQSDIVHSSNTQIKQQCVESLANAGETRKKQELSCERSQEEKDFTQSGALGPKTRSQKSPYGHSELHIESSQISNEPSNVTKQVEVRRSRRLLMLPKGPGNKSNSNAVKEMNEQENIAQIPEFRVKDTNKNNEISDTVPSQLKKKDTYFPPCTSEDTGELENGIPVRKISDQNASLDNPIDPCKDEYSDTLLHVAGDNVQPDYMETEESELETQHIVKMFKTSKRTSFILESKEAENENRVNSAVEISQVETSNELPNVAECRHTSLLSSAKEQSGALLKQGSPSSEPKKTSPIHMLKKTESKHSKMSRNRRGKVKPSSNSAKNTTGQPDNLNNPTQGVTGSLYNKQVMSDFPMRLNVDEEHTNASAKGSQSSVADKSTAHSNVNTDFISDINSATPDGLLHYMDKAEGNSSPWDTTRDKNAALLESCLPSAYGSTGEKTPIPKVQSSEEGSSQGLFNQKPKCSSSGKADQKNSSKNPGKSQCRILSDFSGSSNNGKCSSQEMLSKAAFPSLSQESQCSVSLFSTQSNMSQQSVDEDHKQDVLQDELVSPNKTKRTAISGTEETQISPLRDQYQNPDIDEASECESEASHTGDSSILSSQDELLNTQQRNYMKDSLKKLQQEMAALEAVLGQHGTQKLEAETTCIPSSEHVTEMQEATEEEEEETYQGENLFVKTPPEHVANGSTLIGQSTDNGLQGRNRPVSPSFLCQTRIEKDTPEIVCPAMGISKKSLLTQKELPSQHKQEIRPDLETVNVTPTNPIDAQESLRQSSKNKRGSRMLHNKRSTSNQGSFCLSVEAAESPQIPKQNKAEFGIARKSTSPTFASPARAKVPSVGFKSPVVSSRRNLSFVASGLNQCEMALVQRFSKTTQSILSSRITDSTTHVIMKTDAELVCERTLKYFQGIASRKWVVSYEWVVQSFREGQILDEYDFEVKGDVINGRNHRGPRRSRLGSDGLLLIDFEICFFGSFTDMTLDDLEWMVSECGATVVKKLQFFKKKHNVTSLVIVQPDASTEVRDYTEIRKKHKALVVTREWLMDSVATYRLQKFDAYLA B65951D7FA37D255 q90x95_xenla bard1.L BARD1 bard1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MLLRVRSGNRPSETAVREELPMMDEPGWERTRDALREMERKLCCSKCMFILKDPVCLGGCEHVFCWTCVNENLGNECPLCNTPAWVRDVQVNRQLDNMIQLCAKLRNLLSMGKTDENKMDLCQDTLLRLNPAQEKQKKKQIKMWFSPRSRKVRCVVEKKEKSQQISSKTLPQMASYEFVSDSPDGEPAQKKPDPKPRKRMKKKLKDINAEWGVDTENEIDEVSNPRKEHKSGKSVSFCSSAVIQHSPEVLVVESSLKNDTPEEISPNWNKCGLDEPMEMQSTSMKEVNCPSPSEQSTTLKTQDKEKKITTVKRKKHSSPIASSKRPRRRSSDTSKPLNDSDIKANSPEKACKTVSPHKPSENSAKDVRIESTQAAKRDTKMCSTPLPCAKQNNSAKLGQKSTAMPISPNNLTNVKRNYKGETMLHLASIKGDIQGVEDLLNSGANPNVKDNAGWTPLHEACNLGHTMVVQLLLQHHALMNTTGYQNDTPLHDAVKNGHIAIVKLLLSHGASRDAVNIFGLQPVDYAETEEMKSVLLETQTERDRLLLHPCLAAPQLSSHQRKEEAVVLIASGLLATQRADLTKLAKMLKASICAEYDSTVTHVIVSDEPVLRTMKCMMGILAGCWTLQFSWVKACLQSCNREPEESYEIPHGPHRARLNGQQMLPQLLDGCHFYFLGCFTEHRKEDLIELVKAAGGQILIRQPKPDSDVTQTINTVAYHAEADSDQRFCTQYIVFDRASKYRPERVRQGKVWFAPSSWIIECITSFQLLPVH 2BA4E6DDD56117B3 brca1-bard1 1 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 1B These results imply that nearly all of the BRCA1 and BARD1 in Xenopus egg extracts exists in a heterodimeric complex throughout the cell cycle. brca1-bard1-1 1 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 1B These results imply that nearly all of the BRCA1 and BARD1 in Xenopus egg extracts exists in a heterodimeric complex throughout the cell cycle. brca1-numa 2 5 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait association association 6A BRCA1 immunoprecipitates (IPs) for the presence of TPX2, NuMA, and XRHAMM. Both NuMA and XRHAMM were significantly enriched in these fractions compared to control IPs brca1-rhamm 2 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait association association 6B brca1-tpx2 2 4 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 6B Although TPX2 was not enriched in anti-BRCA1 IPs (Figure 6A), an identical experiment performed with TPX2 Ab led to specific coimmunoprecipitation of BRCA1/BARD1 (Figure 6B, lanes 5 and 6 versus 1 and 2). brca1-numa-1 2 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait association association 6C BRCA1/BARD1 Interacts with Spindle-Pole-Organizing Proteins