IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Crystal structures of a ligand-free MthK gating ring: insights into the ligand gating mechanism of K+ channels. Crystal structures of a ligand-free MthK gating ring: insights into the ligand gating mechanism of K+ channels. Cell (0092-8674) 2006 imex curation Ye S., Li Y., Chen L., Jiang Y. youxing.jiang@utsouthwestern.edu Only protein-protein interactions 3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ye S., Li Y., Chen L., Jiang Y. youxing.jiang@utsouthwestern.edu Accepted 2006-OCT-9 by JYOTI. Cell (0092-8674) 2006 Only protein-protein interactions 3 imex curation Crystal structures of a ligand-free MthK gating ring: insights into the ligand gating mechanism of K+ channels. Crystal structures of a ligand-free MthK gating ring: insights into the ligand gating mechanism of K+ channels. Cell (0092-8674) 2006 imex curation Ye S., Li Y., Chen L., Jiang Y. youxing.jiang@utsouthwestern.edu Only protein-protein interactions 3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ye S., Li Y., Chen L., Jiang Y. youxing.jiang@utsouthwestern.edu Accepted 2006-OCT-9 by JYOTI. Cell (0092-8674) 2006 Only protein-protein interactions 3 imex curation mthk_metth Calcium-gated potassium channel MthK mthK MTH_1520 protein protein metth Methanobacterium thermoautotrophicum Methanobacterium thermoautotrophicum MVLVIEIIRKHLPRVLKVPATRILLLVLAVIIYGTAGFHFIEGESWTVSLYWTFVTIATVGYGDYSPSTPLGMYFTVTLIVLGIGTFAVAVERLLEFLINREQMKLMGLIDVAKSRHVVICGWSESTLECLRELRGSEVFVLAEDENVRKKVLRSGANFVHGDPTRVSDLEKANVRGARAVIVDLESDSETIHCILGIRKIDESVRIIAEAERYENIEQLRMAGADQVISPFVISGRLMSRSIDDGYEAMFVQDVLAEESTRRMVEVPIPEGSKLEGVSVLDADIHDVTGVIIIGVGRGDELIIDPPRDYSFRAGDIILGIGKPEEIERLKNYISA 31FDC5811CB79253 mthk 2 3 unspecified role unspecified role neutral component neutral component glu215 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain c-terminal RCK domain sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain O27564:p.Asp184Asn mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D N Wed May 02 16:21:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp184asn' Tue Jun 30 19:13:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp184Asn' ile192 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain his193 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain his193 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain asp163 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role neutral component neutral component arg213 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain val167 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain c-terminal RCK domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain O27564:p.Asp184Asn mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D N Wed May 02 19:40:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp184asn' Tue Jun 30 18:50:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp184Asn' leu196 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain ser189 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain asn216 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role neutral component neutral component c-terminal RCK domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain O27564:p.Asp184Asn mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D N Wed May 02 18:35:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp184asn' Tue Jun 30 18:35:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp184Asn' glu125 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain lys150 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain arg154 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain ile192 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain his193 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain his193 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role neutral component neutral component c-terminal RCK domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain O27564:p.Asp184Asn mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D N Wed May 02 15:25:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp184asn' Tue Jun 30 19:03:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp184Asn' arg166 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain glu146 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain his161 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain leu196 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain ser189 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain asn216 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 15 9 18 19 10 20 11 14 12 33 24 25 34 26 35 36 27 28 37 29 38 direct interaction direct interaction 1, Table S1 The crystal was of space group C2221 with unit-cell dimensions of a = 174.1A� , b = 181.0A� , c = 252.7A� , a = b = g = 90 and contained eight RCK subunits in one asymmetric unit. All of the bonding between the amino acids specified is stated to occur by the authors. However, it is not clear in all cases which bonds coexist in one particular subunit. mthk-1 1 3 unspecified role unspecified role neutral component neutral component c-terminal RCK domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association As shown in Figure 6A, purified RCK domains remain octameric at pH 7.5, 6 A The RCK domains formed octamers at pH 7.5 and dimers at pH 6.5. A mixture of theswe two forms was found at pH 7.0. mthk-2 1 3 unspecified role unspecified role neutral component neutral component c-terminal RCK domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association As shown in Figure 6A, purified RCK domains remain octameric at pH 7.5, become predominantly dimeric at pH 6.5, 6 A The RCK domains formed octamers at pH 7.5 and dimers at pH 6.5. A mixture of theswe two forms was found at pH 7.0.