IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Arabidopsis PEX19 is a dimeric protein that binds the peroxin PEX10. Arabidopsis PEX19 is a dimeric protein that binds the peroxin PEX10. Mol. Membr. Biol. (0968-7688) 2006 imex curation Hadden DA., Phillipson BA., Johnston KA., Brown LA., Manfield IW., El-Shami M., Sparkes IA., Baker A. Arabidopsis cell cultures were used as the source of protein extracts in these studies. d.hadden@shu.ac.uk 27-NOV-2006: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hadden DA., Phillipson BA., Johnston KA., Brown LA., Manfield IW., El-Shami M., Sparkes IA., Baker A. Arabidopsis cell cultures were used as the source of protein extracts in these studies. d.hadden@shu.ac.uk Accepted 2006-NOV-16 by JYOTI. 27-NOV-2006: Contacted by JYOTI. Mol. Membr. Biol. (0968-7688) 2006 Only protein-protein interactions imex curation Arabidopsis PEX19 is a dimeric protein that binds the peroxin PEX10. Arabidopsis PEX19 is a dimeric protein that binds the peroxin PEX10. Mol. Membr. Biol. (0968-7688) 2006 imex curation Hadden DA., Phillipson BA., Johnston KA., Brown LA., Manfield IW., El-Shami M., Sparkes IA., Baker A. Arabidopsis cell cultures were used as the source of protein extracts in these studies. d.hadden@shu.ac.uk 27-NOV-2006: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Hadden DA., Phillipson BA., Johnston KA., Brown LA., Manfield IW., El-Shami M., Sparkes IA., Baker A. Arabidopsis cell cultures were used as the source of protein extracts in these studies. d.hadden@shu.ac.uk Accepted 2006-NOV-16 by JYOTI. 27-NOV-2006: Contacted by JYOTI. Mol. Membr. Biol. (0968-7688) 2006 Only protein-protein interactions imex curation Arabidopsis PEX19 is a dimeric protein that binds the peroxin PEX10. Arabidopsis PEX19 is a dimeric protein that binds the peroxin PEX10. Mol. Membr. Biol. (0968-7688) 2006 imex curation Hadden DA., Phillipson BA., Johnston KA., Brown LA., Manfield IW., El-Shami M., Sparkes IA., Baker A. Arabidopsis cell cultures were used as the source of protein extracts in these studies. d.hadden@shu.ac.uk 27-NOV-2006: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Hadden DA., Phillipson BA., Johnston KA., Brown LA., Manfield IW., El-Shami M., Sparkes IA., Baker A. Arabidopsis cell cultures were used as the source of protein extracts in these studies. d.hadden@shu.ac.uk Accepted 2006-NOV-16 by JYOTI. 27-NOV-2006: Contacted by JYOTI. Mol. Membr. Biol. (0968-7688) 2006 Only protein-protein interactions imex curation Arabidopsis PEX19 is a dimeric protein that binds the peroxin PEX10. Arabidopsis PEX19 is a dimeric protein that binds the peroxin PEX10. Mol. Membr. Biol. (0968-7688) 2006 imex curation Hadden DA., Phillipson BA., Johnston KA., Brown LA., Manfield IW., El-Shami M., Sparkes IA., Baker A. Arabidopsis cell cultures were used as the source of protein extracts in these studies. d.hadden@shu.ac.uk 27-NOV-2006: Contacted by JYOTI. Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel western blot western blot Immuno blot Immuno blot Hadden DA., Phillipson BA., Johnston KA., Brown LA., Manfield IW., El-Shami M., Sparkes IA., Baker A. Arabidopsis cell cultures were used as the source of protein extracts in these studies. d.hadden@shu.ac.uk Accepted 2006-NOV-16 by JYOTI. 27-NOV-2006: Contacted by JYOTI. Mol. Membr. Biol. (0968-7688) 2006 Only protein-protein interactions imex curation pe191_arath Peroxisome biogenesis protein 19-1 PEX19-1 T21P5.9 At3g03490 Peroxin-19-1 Peroxisomal membrane protein import receptor PEX19-1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MANSHTDDLDELLDSALDDFKDLNLSHQRNQREAQEEEEKKRKEETVLLPSGVQGLGMGLPDMRSKKRGKQKVSKEDHVAEALDKLREQTRETVKGLESISSKQLPASDDDGMVEDFLKQFEDLAGSKDLESIVETMMQQLLSKDILHEPMKELGARYPKWLKENEASLSKEDYKRYSQQYKLIEELNAVYENEPNNSSKIMEIMQKMQECGQPPSDIVKEIDPGFDFASLGQISPEMLESSPNCCIM 90A873E635361140 pex10_arath Peroxisome biogenesis factor 10 PEX10 At2g26350 T9J22.2 Pex10p PER10 Peroxin-10 Peroxisome assembly protein 10 Peroxisomal biogenesis factor 10 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MRLNGDSGPGQDEPGSSGFHGGIRRFPLAAQPEIMRAAEKDDQYASFIHEACRDAFRHLFGTRIALAYQKEMKLLGQMLYYVLTTGSGQQTLGEEYCDIIQVAGPYGLSPTPARRALFILYQTAVPYIAERISTRAATQAVTFDESDEFFGDSHIHSPRMIDLPSSSQVETSTSVVSRLNDRLMRSWHRAIQRWPVVLPVAREVLQLVLRANLMLFYFEGFYYHISKRASGVRYVFIGKQLNQRPRYQILGVFLLIQLCILAAEGLRRSNLSSITSSIQQASIGSYQTSGGRGLPVLNEEGNLITSEAEKGNWSTSDSTSTEAVGKCTLCLSTRQHPTATPCGHVFCWSCIMEWCNEKQECPLCRTPNTHSSLVCLYHSDF 41AFF2C25E65584A pex19 1 5 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 4A Recombinant HisPEX19-1 forms a dimer. The molecular mass was estimated as 60kDa. pex19-1 4 5 unspecified role unspecified role neutral component neutral component arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association 4C PEX19 most likely forms a disulphide-bonded dimer. The authors note that two immuno reactive species were seen with the faster migrating species increased slightly in abundance when samples were extracted in the presence of DTT. pex19-pex10 2 6 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 5 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminal t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range in vitro In vitro direct interaction direct interaction 5 In vitro translated AtPEX19 interacts with PEX10. pex19-pex10-2 3 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role prey prey n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) physical association physical association 6 Recombinant HisPEX19-1 bind to the C-terminus of PEX10.