IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Homeodomain-mediated beta-catenin-dependent switching events dictate cell-lineage determination. Homeodomain-mediated beta-catenin-dependent switching events dictate cell-lineage determination. Cell (0092-8674) 2006 imex curation Olson LE., Tollkuhn J., Scafoglio C., Krones A., Zhang J., Ohgi KA., Wu W., Taketo MM., Kemler R., Grosschedl R., Rose D., Li X., Rosenfeld MG. sean.li@childrens.harvard.edu,mrosenfeld@ucsd.edu 240..240 240..250 2006-06-28: Only interaction involving solely Proteins were exported. Only protein-protein interactions 2 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Olson LE., Tollkuhn J., Scafoglio C., Krones A., Zhang J., Ohgi KA., Wu W., Taketo MM., Kemler R., Grosschedl R., Rose D., Li X., Rosenfeld MG. Immobilized GST-fusion proteins were mixed with 293T cell lysates containing overexpressed, tagged beta-catenin protein. sean.li@childrens.harvard.edu,mrosenfeld@ucsd.edu Accepted 2006-JUN-9 by DAVET. Cell (0092-8674) 2006 240..240 240..250 2006-06-28: Only interaction involving solely Proteins were exported. Only protein-protein interactions 2 imex curation Homeodomain-mediated beta-catenin-dependent switching events dictate cell-lineage determination. Homeodomain-mediated beta-catenin-dependent switching events dictate cell-lineage determination. Cell (0092-8674) 2006 imex curation Olson LE., Tollkuhn J., Scafoglio C., Krones A., Zhang J., Ohgi KA., Wu W., Taketo MM., Kemler R., Grosschedl R., Rose D., Li X., Rosenfeld MG. sean.li@childrens.harvard.edu,mrosenfeld@ucsd.edu 240..240 240..250 2006-06-28: Only interaction involving solely Proteins were exported. Only protein-protein interactions 2 mouse-ghft_1 Mus musculus immortalised somatotropic progenitor cells - SV40 transformed. ghft_1 mouse immortalized somatotropic progenitor cell anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Olson LE., Tollkuhn J., Scafoglio C., Krones A., Zhang J., Ohgi KA., Wu W., Taketo MM., Kemler R., Grosschedl R., Rose D., Li X., Rosenfeld MG. sean.li@childrens.harvard.edu,mrosenfeld@ucsd.edu Accepted 2006-JUN-9 by DAVET. Cell (0092-8674) 2006 240..240 240..250 2006-06-28: Only interaction involving solely Proteins were exported. Only protein-protein interactions 2 imex curation ctnb1_mouse Catenin beta-1 Beta-catenin Ctnnb1 Catnb protein protein mouse Mus musculus Mouse MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEALGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL DIP protein Q02248 original sequence version: 128 DIP protein Q02248 original sequence version: 135 DIP protein Q02248 original sequence version: 147 DIP protein Q02248 original sequence version: 187 DIP protein Q02248 original sequence version: 140 D708F170A3FBED6E prop1_mouse Homeobox protein prophet of Pit-1 Prop1 Prop-1 Pituitary-specific homeodomain factor protein protein mouse Mus musculus Mouse MEAQRRSHQEKQTKGHACGRSLPEPRVASGTLISTVDRSSEAYRRLSGTELGRPKLCPQRGRPHSRRRHRTTFNPAQLEQLESAFGRNQYPDIWAREGLAQDTGLSEARIQVWFQNRRAKQRKQERSLLQPIAHLSTATFSGFLPESSAYPYTYGTPPPPAPCFPHPYSHSLPSQPSTAASLALPPQPEDWYPTLHPAPTGHLPCPPPPPMFPLSLETPKSWN CD0AC4DFDC383679 prop1-catenine 2 4 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey physical association physical association 4D To establish that these interactions between Prop1 and β-catenin holoproteins occur in a cellular context, we performed coimmunoprecipitation in GHFT-1 cells, prop1-catenine-1 1 4 unspecified role unspecified role prey prey c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain homeodomain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined lef1 region Arm repeats 5-9 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 4C The C-terminal 60 aa of Prop1 interacted robustly with the full-length β-catenin protein in a GST pull-down protein-protein interaction assay The authors have described Prop1 protein with 225 AA while the swissprot entry points to an 223 AA sequence. An TrEMBL enty Q8CJ18 corresponding to 224 AA does exist. The sequences are most distinct in their C-terminal end which is the interacting region. Homeodomain of Prop1 shows weaker interaction than C-terminal. Q02248 overexpressed