MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it YIL042c and YOR090c encode the kinase and phosphatase of the Saccharomyces cerevisiae pyruvate dehydrogenase complex. YIL042c and YOR090c encode the kinase and phosphatase of the Saccharomyces cerevisiae pyruvate dehydrogenase complex. FEBS Lett. (0014-5793) 2006 imex curation Krause-Buchholz U., Gey U., Wünschmann J., Becker S., Rödel G. ukrause@rcs.urz.tu-dresden.de 2009-01-08 Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast density sedimentation cosedimentation through density gradient density sedimentation nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Krause-Buchholz U., Gey U., Wünschmann J., Becker S., Rödel G. ukrause@rcs.urz.tu-dresden.de FEBS Lett. (0014-5793) 2006 2009-01-08 Only protein-protein interactions imex curation YIL042c and YOR090c encode the kinase and phosphatase of the Saccharomyces cerevisiae pyruvate dehydrogenase complex. YIL042c and YOR090c encode the kinase and phosphatase of the Saccharomyces cerevisiae pyruvate dehydrogenase complex. FEBS Lett. (0014-5793) 2006 imex curation Krause-Buchholz U., Gey U., Wünschmann J., Becker S., Rödel G. ukrause@rcs.urz.tu-dresden.de 2009-01-08 Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast tap tandem affinity purification TAP TAP tap nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Krause-Buchholz U., Gey U., Wünschmann J., Becker S., Rödel G. ukrause@rcs.urz.tu-dresden.de FEBS Lett. (0014-5793) 2006 2009-01-08 Only protein-protein interactions imex curation odpa_yeast Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial YER178W PDA1 Pyruvate dehydrogenase complex component E1 alpha protein protein yeast Saccharomyces cerevisiae Baker's yeast MLAASFKRQPSQLVRGLGAVLRTPTRIGHVRTMATLKTTDKKAPEDIEGSDTVQIELPESSFESYMLEPPDLSYETSKATLLQMYKDMVIIRRMEMACDALYKAKKIRGFCHLSVGQEAIAVGIENAITKLDSIITSYRCHGFTFMRGASVKAVLAELMGRRAGVSYGKGGSMHLYAPGFYGGNGIVGAQVPLGAGLAFAHQYKNEDACSFTLYGDGASNQGQVFESFNMAKLWNLPVVFCCENNKYGMGTAASRSSAMTEYFKRGQYIPGLKVNGMDILAVYQASKFAKDWCLSGKGPLVLEYETYRYGGHSMSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLIDLGIATEAEVKAYDKSARKYVDEQVELADAAPPPEAKLSILFEDVYVKGTETPTLRGRIPEDTWDFKKQGFASRD 37019BD9E84D0002 37019BD9E84D0002 pdp1_yeast [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial PTC5 Phosphatase two C protein 5 PPP1 Protein phosphatase 2C homolog 5 Protein phosphatase of PDH protein 1 Pyruvate dehydrogenase complex phosphatase 1 YOR3157C YOR090C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSPLTRTVAIKKTVKVLSKCQSGREYTQKFLQRAYSTSHANSTYYSRTKLFISSHSKALNIALLSGSLLLTYSYYSPKKILSLDTINGIKDYSTNTSGNINMPSPNPKGTETQKSQRSQNDQSVLILNDSKIEAKLHDREESHFVNRGTGIFRYDVAQLPSNHPIEDDHVEQIITIPIESEDGKSIEKDLYFFGIFDGHGGPFTSEKLSKDLVRYVAYQLGQVYDQNKTVFHSDPNQLIDSAISKGFLKLDNDLVIESFRKLFQDPNNTNIANTLPAISGSCALLSLYNSTNSILKVAVTGDSRALICGLDNEGNWTVKSLSTDQTGDNLDEVRRIRKEHPGEPNVIRNGRILGSLQPSRAFGDYRYKIKEVDGKPLSDLPEVAKLYFRREPRDFKTPPYVTAEPVITSAKIGENTKFMVMGSDGLFELLTNEEIASLVIRWMDKNMNLAPVKAEPGKLPKVIDVSEDKEAQRPAFRYKDNNSSSPSGSNPEYLIEDKNVATHLIRNALSAGGRKEYVSALVSIPSPMSRRYRDDLTVTVAFFGDSGTPSIVSNATSIVMNPEATTKPKPRL 07A5AF33FA72CC2A 07A5AF33FA72CC2A pdk1_yeast [Pyruvate dehydrogenase (acetyl-transferring)] kinase 1, mitochondrial PKP1 Protein kinase of PDH protein 1 Pyruvate dehydrogenase complex kinase 1 [Pyruvate dehydrogenase [lipoamide]] kinase 1 YIL042C protein protein yeast Saccharomyces cerevisiae Baker's yeast MWKIMRSWKCGGMRWAHRQRPSHELLSQLSFDQHYKIRSNIELLIQDYASKPIAPLNYEYFLQYRPPLTKKEEYMLTIKTINLLLSLTCKRLNAIQRLPYNAVINPHIERTNSLYLKSLQTLLSIAYPYELHNPPKIQAKFTELLDDHEDAIVVLAKGLQEIQSCYPKFQISQFLNFHLKERITMKLLVTHYLSLMAQNKGDTNKRMIGILHRDLPIAQLIKHVSDYVNDICFVKFNTQRTPVLIHPPSQDITFTCIPPILEYIMTEVFKNAFEAQIALGKEHMPIEINLLKPDDDELYLRIRDHGGGITPEVEALMFNYSYSTHTQQSADSESTDLPGEQINNVSGMGFGLPMCKTYLELFGGKIDVQSLLGWGTDVYIKLKGPSKTALLSKK B2A996C089606961 acon2_yeast Homocitrate dehydratase, mitochondrial ACO2 Aconitase 2 J0327 YJL200C protein protein yeast Saccharomyces cerevisiae Baker's yeast MLSSANRFYIKRHLATHANMFPSVSKNFQTKVPPYAKLLTNLDKIKQITNNAPLTLAEKILYSHLCDPEESITSSDLSTIRGNKYLKLNPDRVAMQDASAQMALLQFMTTGLNQTSVPASIHCDHLIVGKDGETKDLPSSIATNQEVFDFLESCAKRYGIQFWGPGSGIIHQIVLENFSAPGLMMLGTDSHTPNAGGLGAIAIGVGGADAVDALTGTPWELKAPKILGVKLTGKLNGWSTPKDVITKLAGLLTVRGGTGYIVEYFGEGVSTLSCTGMATICNMGAEIGATTSTFPYQEAHKRYLQATNRAEVAEAADVALNKFNFLRADKDAQYDKVIEIDLSAIEPHVNGPFTPDLSTPISQYAEKSLKENWPQKVSAGLIGSCTNSSYQDMSRVVDLVKQASKAGLKPRIPFFVTPGSEQIRATLERDGIIDIFQENGAKVLANACGPCIGQWNREDVSKTSKETNTIFTSFNRNFRARNDGNRNTMNFLTSPEIVTAMSYSGDAQFNPLTDSIKLPNGKDFKFQPPKGDELPKRGFEHGRDKFYPEMDPKPDSNVEIKVDPNSDRLQLLEPFKPWNGKELKTNVLLKVEGKCTTDHISAAGVWLKYKGHLENISYNTLIGAQNKETGEVNKAYDLDGTEYDIPGLMMKWKSDGRPWTVIAEHNYGEGSAREHAALSPRFLGGEILLVKSFARIHETNLKKQGVLPLTFANESDYDKISSGDVLETLNLVDMIAKDGNNGGEIDVKITKPNGESFTIKAKHTMSKDQIDFFKAGSAINYIGNIRRNE FABA4FE482D3F993 cox20_yeast Cytochrome c oxidase assembly protein COX20, mitochondrial COX20 YD9934.15c YDR231C protein protein yeast Saccharomyces cerevisiae Baker's yeast MRWWPWSNQTEDQKQQQQPQGKADGDRVLTNYSRGQKILLEDTPPKFADDLSNSQLAKKQERATLKEAWDSIRWSDFSLQKLTSIPCFRDAGMLGFSSMFLMGSIIFIYHKSPTKATNWAMSSLILGSIVGWEQCRLKRQKSFQIAQLAKETVAKKEKPMLHNVPHDPSLPGQWEAAKNEKQSQFEQSNQNLSQASSEKKWYKFW 1E1893856FF8456D pda1-pkp1 2 3 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tap tagged tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 5 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell myc tagged myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 physical association physical association F7B proteins of a strain coexpressing Pda1p-TAP and Yil042c-cMyc were lysed and subjected to the two consecutive affinity steps FEBS-Letters mint aco2-cox20 1 6 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 7 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level 4 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed ha tagged ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 5 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed myc tagged myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 colocalization colocalization F3 EMBO-Journal The purity of the obtained fractions was assessed by detection of the cytosolic phosphoglycerate kinase (Pgk1p), the inner mt membrane protein Cox2p (subunit of cytochrome c oxidase, COX) and the soluble mt matrix enzyme aconitase (Aco1p). In line with previous studies [18], [19], [20] and [21], the majority of Yil042cp and Yor090cp was detected in the mt fractions (Fig. 3). mint