MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Analysis of KaiA-KaiC protein interactions in the cyano-bacterial circadian clock using hybrid structural methods. Analysis of KaiA-KaiC protein interactions in the cyano-bacterial circadian clock using hybrid structural methods. EMBO J. (0261-4189) 2006 imex curation Pattanayek R., Williams DR., Pattanayek S., Xu Y., Mori T., Johnson CH., Stewart PL., Egli M. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species Native gel-electrophoretic assay using 4-15 % gradient gel. Fig 4 martin.egli@vanderbilt.edu 26-JUL-2004: Contacted by JYOTI Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Pattanayek R., Williams DR., Pattanayek S., Xu Y., Mori T., Johnson CH., Stewart PL., Egli M. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species Native gel-electrophoretic assay using 4-15 % gradient gel. Fig 4 martin.egli@vanderbilt.edu Accepted 2006-JUL-17 by JYOTI. 26-JUL-2004: Contacted by JYOTI EMBO J. (0261-4189) 2006 Only protein-protein interactions imex curation Analysis of KaiA-KaiC protein interactions in the cyano-bacterial circadian clock using hybrid structural methods. Analysis of KaiA-KaiC protein interactions in the cyano-bacterial circadian clock using hybrid structural methods. EMBO J. (0261-4189) 2006 imex curation Pattanayek R., Williams DR., Pattanayek S., Xu Y., Mori T., Johnson CH., Stewart PL., Egli M. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species Native gel-electrophoretic assay using 4-15 % gradient gel. Fig 4 martin.egli@vanderbilt.edu 26-JUL-2004: Contacted by JYOTI Only protein-protein interactions in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Pattanayek R., Williams DR., Pattanayek S., Xu Y., Mori T., Johnson CH., Stewart PL., Egli M. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species Native gel-electrophoretic assay using 4-15 % gradient gel. Fig 4 martin.egli@vanderbilt.edu Accepted 2006-JUL-17 by JYOTI. 26-JUL-2004: Contacted by JYOTI EMBO J. (0261-4189) 2006 Only protein-protein interactions imex curation Analysis of KaiA-KaiC protein interactions in the cyano-bacterial circadian clock using hybrid structural methods. Analysis of KaiA-KaiC protein interactions in the cyano-bacterial circadian clock using hybrid structural methods. EMBO J. (0261-4189) 2006 imex curation Pattanayek R., Williams DR., Pattanayek S., Xu Y., Mori T., Johnson CH., Stewart PL., Egli M. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species Native gel-electrophoretic assay using 4-15 % gradient gel. Fig 4 martin.egli@vanderbilt.edu 26-JUL-2004: Contacted by JYOTI Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel western blot western blot Immuno blot Immuno blot Pattanayek R., Williams DR., Pattanayek S., Xu Y., Mori T., Johnson CH., Stewart PL., Egli M. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species Native gel-electrophoretic assay using 4-15 % gradient gel. Fig 4 martin.egli@vanderbilt.edu Accepted 2006-JUL-17 by JYOTI. 26-JUL-2004: Contacted by JYOTI EMBO J. (0261-4189) 2006 Only protein-protein interactions imex curation kaic_theeb Circadian clock protein kinase KaiC tlr0483 kaiC protein protein theeb Thermosynechococcus elongatus (strain BP-1) MTNLPEHQSSPTEQSSAEVKKIPTMIEGFDDISHGGLPQGRTTLVSGTSGTGKTLFAVQFLYNGITIFNEPGIFVTFEESPQDIIKNALSFGWNLQSLIDQGKLFILDASPDPDGQEVAGDFDLSALIERIQYAIRKYKATRVSIDSVTAVFQQYDAASVVRREIFRLAFRLKQLGVTTIMTTERVDEYGPVARFGVEEFVSDNVVILRNVLEGERRRRTVEILKLRGTTHMKGEYPFTINNGINIFPLGAMRLTQRSSNVRVSSGVKTLDEMCGGGFFKDSIILATGATGTGKTLLVSKFLETGCQQGERALLFAYEESRAQLSRNASSWGIDFEELERRGLLRIICAYPESAGLEDHLQIIKSEIADFKPSRVAIDSLSALARGVSNNAFRQFVIGVTGFAKQEEITGFFTNTTDQFMGSNSITESHISTITDTILLLQYVEIRGEMSRAINVFKMRGSWHDKGIREYVITEKGAEIRDSFRNFEGIISGTPTRISVDEKTELARIAKGMQDLESE 34E4CDF9DE9C1824 34E4CDF9DE9C1824 kaia_theeb Circadian clock protein KaiA kaiA tlr0481 protein protein theeb Thermosynechococcus elongatus (strain BP-1) MAQSTALTICGLVYSPAIGQELVRLHTSDIDELVYFSSEREFCNYLEARRNSIACLILEWGEGTPQIITYLHHSATLLPAILIFPAAPAPPPAGPHYHIAEVILTTDQLDQLNRQIEEAITGFVKLCPGCAVPPHVLFRLPALKESSNVDPQHRLSQKLKERLGYLGVYYKRDTAFFFRRMSPADKRKLLDELRSIYRTIVLEYFNTDAKVNERIDEFVSKAFFADISVSQVLEIHVELMDTFSKQLKLEGRSEDILLDYRLTLIDVIAHLCEMYRRSIPREV D3B0DCBE8A580B4E D3B0DCBE8A580B4E kaia_thevl Circadian clock protein KaiA kaiA protein protein thevl Thermosynechococcus vulcanus Synechococcus vulcanus MAQSTALTICGLVYSPAIGQELVRLHTSDIDELVYFSSEREFCNYLEARRNSVACLILEWGEGTPQIITYLHHSATLLPAILIFPAAPAPPPAGPHYHIAEVILTTDQLDQLNRQIEEAITGFVKLCPGCAVPPHVLFRLPALKESSNVDPQHRLSQKLKERLGYLGVYYKRDTAFFFRRMSPADKRKLLDELRSIYRTIVLEYFNTDAKVNERIDEFVSKAFFADISVSQVLEIHVELMDTFSKQLKLEGRSEDILLDYRLTLIDVIAHLCEMYRRSIPREV 2BC455DB1C016A6F kaic_thevl Circadian clock protein kinase KaiC kaiC protein protein thevl Thermosynechococcus vulcanus Synechococcus vulcanus MTNLPEHQSSPTEQSSAEVKKIPTMIEGFDDISHGGLPQGRTTLVSGTSGTGKTLFAVQFLYNGITIFNEPGIFVTFEESPQDIIKNALSFGWNLQSLIDQGKLFILDASPDPDGQEVAGDFDLSALIERIQYAIRKYKATRVSIDSVTAVFQQYDAASVVRREIFRLAFRLKQLGVTTIMTTERVDEYGPVARFGVEEFVSDNVVILRNVLEGERRRRTVEILKLRGTTHMKGEYPFTINNGINIFPLGAMRLTQRSSNVRVSSGVKTLDEMCGGGFFKDSIILATGATGTGKTLLVSKFLETGCQQGERALLFAYEESRAQLSRNASSWGIDFEELERRGLLRIICAYPESAGLEDHLQIIKSEIADFKPSRVAIDSLSALARGVSNNAFRQFVIGVTGFAKQEEITGFFTNTTDQFMGSNSITESHISTITDTILLLQYVEIRGEMSRAINVFKMRGSWHDKGIREYVITEKGAEIRDSFRNFEGIISGTPTRISVDEKTELARIAKGMQDLESE 34E4CDF9DE9C1824 kaia-kaia 1 5 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction kaia-kaia-5 1 6 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction kaic-kaic-12 1 7 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction hexamerization was induced by addition of AMPPNP. kaia-kaic-16 1 6 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminus required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Unlike Synechoccus elongatus KaiC, Thermosynechoccus KaiC can be purified as monomer. Thus Phosphorylation with ATP and hexamerization with AMPPNP can be performed separetely making isolation of a stable KaiA-KaiC complex possible. The c-terminal 25 amino-acids linker of KaiC is necessary for kaiA dimer binding to KaiC hexamer. kaic-kaic-13 1 4 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Hexamerization was induced by addition of AMPPNP. kaic-kaic-14 3 4 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Hexamerization was induced by addition of ATP. KaiC c-terminus deletion which impairs circadian rythm in vivo does not the kaiC hexamer formation in vitro. kaia-kaic-17 2 7 unspecified role unspecified role neutral component neutral component c-terminus required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Unlike Synechoccus elongatus KaiC, Thermosynechoccus KaiC can be purified as monomer. Thus Phosphorylation with ATP and hexamerization with AMPPNP can be performed separetely making isolation of a stable KaiA-KaiC complex possible. The c-terminal 25 amino-acids linker of KaiC is necessary for kaiA dimer binding to KaiC hexamer. kaic-kaic-15 2 7 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction hexamerization was induced by addition of AMPPNP. kaic-kaic-16 2 4 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Hexamerization was induced by addition of AMPPNP.