MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Identification of the basement membrane protein nidogen as a candidate ligand for tumor endothelial marker 7 in vitro and in vivo. Identification of the basement membrane protein nidogen as a candidate ligand for tumor endothelial marker 7 in vitro and in vivo. FEBS Lett. (0014-5793) 2006 imex curation Lee HK., Seo IA., Park HK., Park HT. 2009-01-08 Only protein-protein interactions in vitro In vitro saturation binding saturation binding nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Lee HK., Seo IA., Park HK., Park HT. FEBS Lett. (0014-5793) 2006 2009-01-08 Only protein-protein interactions imex curation Identification of the basement membrane protein nidogen as a candidate ligand for tumor endothelial marker 7 in vitro and in vivo. Identification of the basement membrane protein nidogen as a candidate ligand for tumor endothelial marker 7 in vitro and in vivo. FEBS Lett. (0014-5793) 2006 imex curation Lee HK., Seo IA., Park HK., Park HT. 2009-01-08 Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom predetermined predetermined participant predetermined Lee HK., Seo IA., Park HK., Park HT. FEBS Lett. (0014-5793) 2006 2009-01-08 Only protein-protein interactions imex curation nid1_rat Nidogen-1 Nid1 Nid Entactin protein protein rat Rattus norvegicus (Rat) Rat EFHPGTFPPSFGSVAPFLADLDTTDGLGNVYYREDLSPFIIQMAAEYVQRGFPEVSFQPTSVVVVTWESMAPYGGPSGSLVEEGKRNTFQAVLASSNSSSYAIFLYPDDGLQFFTTFSKKDENQVPAVVGFSKGLEGFLWKSNGAYNIFANDRESIENLAKSSNAGHQGVWVFEIGSPATAKGVVPADVNLDVDDDGADYEDEDYDLQTSHLGLEDVATQPFPSHSPRRGYPDPHNVPRTLAPSYEATERPHGIPTERTKSFQLPVERFPQKHPQVIDVDEVEETGVVFSYNTGSQQTCANNRHQCSVHAECRDYATGFCCRCV fragmentation type: fragment mint Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions CBDEAD3337019027 pldx1_mouse Plexin domain-containing protein 1 Plxdc1 Tem7 Tumor endothelial marker 7 protein protein mouse Mus musculus Mouse MRAQLWLLQLLLLRGAARALSPATPAGHNEGQDSAWTAKRTRQGWSRRPRESPAQVLKPGKTQLSQDLGGGSLAIDTLPDNRTRVVEDNHNYYVSRVYGPGEKQSQDLWVDLAVANRSHVKIHRILSSSHRQASRVVLSFDFPFYGHPLRQITIATGGFIFMGDMLHRMLTATQYVAPLMANFNPGYSDNSTVAYFDNGTVFVVQWDHVYLQDREDRGSFTFQAALHRDGRIVFGYKEIPMAVLDISSAQHPVKAGLSDAFMILNSSPEVPASQRRTIFEYHRVELDSSKITTTSAVEFTPLPTCLQHQSCDTCVSSNLTFNCSWCHVLQRCSSGFDRYRQEWLTYGCAQEAEGKTCEDFQDDSHYSASPDSSFSPFNGDSTTSSSLFIDSLTTEDDTKLNPYAEGDGLPDHSSPKSKGPPVHLGTIVGIVLAVLLVAAIILAGIYISGHPNSNAALFFIERRPHHWPAMKFHNHPNHSTYTEVEPSGHEKEGFVEAEQC mint 802D6865F8CA18BD nid1-plxdc1 2 4 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell Stoichiometry: 1.0 3 polyclonal western polyclonal antibody western blot polyclonal western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell fusion proteins fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 physical association physical association F2A This experiment demonstrated that nickel beads specifically co-precipitated the eTEM7-AP protein and nidogen-Fc, which could be detected by a polyclonal anti-human IgG antibody (Fig. 2A). FEBS-Letters homomint mint nid1-plxdc1-1 1 3 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified fusion proteins fusion proteins fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 4 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified tagged molecule multiple tagged tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 direct interaction direct interaction F2B We next estimated the binding affinity for the interactions between TEM7 ectodomain and nidogen-Fc in an equilibrium binding experiment (Fig. 2B). Specific binding of eTEM7-AP to purified nidogen-Fc showed saturation, yielding values for the Kd of 10.4 nM. kd 10.4 nanomolar FEBS-Letters homomint mint