IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Analysis of the interacting partners of the neuronal calcium-binding proteins L-CaBP1, hippocalcin, NCS-1 and neurocalcin delta. Analysis of the interacting partners of the neuronal calcium-binding proteins L-CaBP1, hippocalcin, NCS-1 and neurocalcin delta. Proteomics (1615-9853) 2006 imex curation Haynes LP., Fitzgerald DJ., Wareing B., O'Callaghan DW., Morgan A., Burgoyne RD. Many proteins were not identified correctly e.g. CAPS1 Q61374 (rabbit), Glycogen synthase 1NOJ (rabbit), Vacuolar ATPase subunit D AAG30726 (human), Tubulin-1 alpha I77403 (human) ACT1 P35432 (Echinococcus granulosus), TCP-1 JC4073 9mouse0, Tubulin-1 alpha I77403 (human), beta-adaptin (mouse) and alpha-adaptin A30111 (mouse). Revisit when proteins are correctly idetified by authors. Bovine brain and membrane protein extracts were prepared from homogenised tissue. CaCL was adde to give a final concentration of 1 micro-molar free calcium ions. Proteins requiring calcium for interaction were eluted with calcium free elution buffer. Table 1. Table 2. Some NCBI accession numbers were mapped manually to Uniprot accession numbers. burgoyne@liv.ac.uk 1481..1488 1481..1498 2006-09-14: Only interaction involving solely Proteins were exported. Only protein-protein interactions 8 in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Haynes LP., Fitzgerald DJ., Wareing B., O'Callaghan DW., Morgan A., Burgoyne RD. Many proteins were not identified correctly e.g. CAPS1 Q61374 (rabbit), Glycogen synthase 1NOJ (rabbit), Vacuolar ATPase subunit D AAG30726 (human), Tubulin-1 alpha I77403 (human) ACT1 P35432 (Echinococcus granulosus), TCP-1 JC4073 9mouse0, Tubulin-1 alpha I77403 (human), beta-adaptin (mouse) and alpha-adaptin A30111 (mouse). Revisit when proteins are correctly idetified by authors. Bovine brain and membrane protein extracts were prepared from homogenised tissue. CaCL was adde to give a final concentration of 1 micro-molar free calcium ions. Proteins requiring calcium for interaction were eluted with calcium free elution buffer. Table 1. Table 2. Some NCBI accession numbers were mapped manually to Uniprot accession numbers. burgoyne@liv.ac.uk Accepted 2006-APR-25 by JYOTI. Proteomics (1615-9853) 2006 1481..1488 1481..1498 2006-09-14: Only interaction involving solely Proteins were exported. Only protein-protein interactions 8 imex curation Analysis of the interacting partners of the neuronal calcium-binding proteins L-CaBP1, hippocalcin, NCS-1 and neurocalcin delta. Analysis of the interacting partners of the neuronal calcium-binding proteins L-CaBP1, hippocalcin, NCS-1 and neurocalcin delta. Proteomics (1615-9853) 2006 imex curation Haynes LP., Fitzgerald DJ., Wareing B., O'Callaghan DW., Morgan A., Burgoyne RD. Many proteins were not identified correctly e.g. CAPS1 Q61374 (rabbit), Glycogen synthase 1NOJ (rabbit), Vacuolar ATPase subunit D AAG30726 (human), Tubulin-1 alpha I77403 (human) ACT1 P35432 (Echinococcus granulosus), TCP-1 JC4073 9mouse0, Tubulin-1 alpha I77403 (human), beta-adaptin (mouse) and alpha-adaptin A30111 (mouse). Revisit when proteins are correctly idetified by authors. Bovine brain and membrane protein extracts were prepared from homogenised tissue. CaCL was adde to give a final concentration of 1 micro-molar free calcium ions. Proteins requiring calcium for interaction were eluted with calcium free elution buffer. Table 1. Table 2. Some NCBI accession numbers were mapped manually to Uniprot accession numbers. burgoyne@liv.ac.uk 1481..1488 1481..1498 2006-09-14: Only interaction involving solely Proteins were exported. Only protein-protein interactions 8 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Haynes LP., Fitzgerald DJ., Wareing B., O'Callaghan DW., Morgan A., Burgoyne RD. Many proteins were not identified correctly e.g. CAPS1 Q61374 (rabbit), Glycogen synthase 1NOJ (rabbit), Vacuolar ATPase subunit D AAG30726 (human), Tubulin-1 alpha I77403 (human) ACT1 P35432 (Echinococcus granulosus), TCP-1 JC4073 9mouse0, Tubulin-1 alpha I77403 (human), beta-adaptin (mouse) and alpha-adaptin A30111 (mouse). Revisit when proteins are correctly idetified by authors. Bovine brain and membrane protein extracts were prepared from homogenised tissue. CaCL was adde to give a final concentration of 1 micro-molar free calcium ions. Proteins requiring calcium for interaction were eluted with calcium free elution buffer. Table 1. Table 2. Some NCBI accession numbers were mapped manually to Uniprot accession numbers. burgoyne@liv.ac.uk Accepted 2006-APR-25 by JYOTI. Proteomics (1615-9853) 2006 1481..1488 1481..1498 2006-09-14: Only interaction involving solely Proteins were exported. Only protein-protein interactions 8 imex curation cabp1_human Calcium-binding protein 1 Calbrain CABP1 Caldendrin protein protein human Homo sapiens Human MGGGDGAAFKRPGDGARLQRVLGLGSRREPRSLPAGGPAPRRTAPPPPGHASAGPAAMSSHIAKSESKTSLLKAAAAAASGGSRAPRHGPARDPGLPSRRLPGSCPATPQSSGDPSSRRPLCRPAPREEGARGSQRVLPQAHCRPREALPAAASRPSPSSPLPPARGRDGEERGLSPALGLRGSLRARGRGDSVPAAASEADPFLHRLRPMLSSAFGQDRSLRPEEIEELREAFREFDKDKDGYINCRDLGNCMRTMGYMPTEMELIELSQQINMNLGGHVDFDDFVELMGPKLLAETADMIGVKELRDAFREFDTNGDGEISTSELREAMRKLLGHQVGHRDIEEIIRDVDLNGDGRVDFEEFVRMMSR 3444721152E01AA7 idh3a_bovin Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial IDH3A Isocitric dehydrogenase subunit alpha NAD(+)-specific ICDH subunit alpha Isocitrate dehydrogenase subunits 3/4 protein protein bovin Bos taurus (Bovine) Bovine MAGPAWISKVSRLLGAFHNQKQVTRGFAGGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVAAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYHDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEAASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGNSKCSDFTEEICRRVKDLD B169D59B30E51C9F hsp7c_bovin Heat shock cognate 71 kDa protein HSPA8 HSC70 Heat shock 70 kDa protein 8 protein protein bovin Bos taurus (Bovine) Bovine MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD DIP protein P19120 original sequence version: 86 FBE109C14A28B925 ncs1_rat Neuronal calcium sensor 1 Ncs1 Flup Freq Frequenin homolog Frequenin-like protein Frequenin-like ubiquitous protein protein protein rat Rattus norvegicus (Rat) Rat MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV 9AF8E26A23F80D4F mbp_bovin Myelin basic protein MBP Myelin A1 protein 20 kDa microtubule-stabilizing protein protein protein bovin Bos taurus (Bovine) Bovine AAQKRPSQRSKYLASASTMDHARHGFLPRHRDTGILDSLGRFFGSDRGAPKRGSGKDGHHAARTTHYGSLPQKAQGHRPQDENPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQKPGFGYGGRASDYKSAHKGLKGHDAQGTLSKIFKLGGRDSRSGSPMARR DIP protein P02687 original sequence version: 75 8E1157B7A1978484 pde1a_bovin Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A PDE1A 61 kDa Cam-PDE protein protein bovin Bos taurus (Bovine) Bovine MGSTATETEELENTTFKYLIGEQTEKMWQRLKGILRCLVKQLEKGDVNVIDLKKNIEYAASVLEAVYIDETRRLLDTDDELSDIQSDSVPSEVRDWLASTFTRKMGMMKKKSEEKPRFRSIVHVVQAGIFVERMYRKSYHMVGLAYPEAVIVTLKDVDKWSFDVFALNEASGEHSLKFMIYELFTRYDLINRFKIPVSCLIAFAEALEVGYSKYKNPYHNLIHAADVTQTVHYIMLHTGIMHWLTELEILAMVFAAAIHDYEHTGTTNNFHIQTRSDVAILYNDRSVLENHHVSAAYRLMQEEEMNVLINLSKDDWRDLRNLVIEMVLSTDMSGHFQQIKNIRNSLQQPEGLDKAKTMSLILHAADISHPAKSWKLHHRWTMALMEEFFLQGDKEAELGLPFSPLCDRKSTMVAQSQIGFIDFIVEPTFSLLTDSTEKIIIPLIEEDSKTKTPSYGASRRSNMKGTTNDGTYSPDYSLASVDLKSFKNSLVDIIQQNKERWKELAAQGEPDPHKNSDLVNAEEKHAETHS 24CF83E5211AE06F atpb_bovin ATP synthase subunit beta, mitochondrial ATP5F1B ATP synthase F1 subunit beta ATP5B protein protein bovin Bos taurus (Bovine) Bovine MLGLVGRVVAASASGALRGLSPSAPLPQAQLLLRAAPAALQPARDYAAQASPSPKAGATTGRIVAVIGAVVDVQFDEGLPPILNALEVQGRETRLVLEVAQHLGESTVRTIAMDGTEGLVRGQKVLDSGAPIRIPVGPETLGRIMNVIGEPIDERGPIKTKQFAAIHAEAPEFVEMSVEQEILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFQVAEVFTGHLGKLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEEHS DIP protein P00829 original sequence version: 152 DIP protein P00829 original sequence version: 93 DIP protein P00829 original sequence version: 92 DIP protein P00829 original sequence version: 109 DIP protein P00829 original sequence version: 125 DIP protein P00829 original sequence version: 134 32218D14A5497F18 acon_bovin Aconitate hydratase, mitochondrial ACO2 Citrate hydro-lyase protein protein bovin Bos taurus (Bovine) Bovine MAPYSLLVSRLQKALGARQYHVASVLCQRAKVAMSHFEPNEYIRYDLLEKNINIVRKRLNRPLTLSEKIVYGHLDDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPSTIHCDHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGRADIANLADEFKDHLVPDSGCHYDQLIEINLSELKPHINGPFTPDLAHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLQCKSQFTITPGSEQIRATIERDGYAQILRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPETDFLTGKDGKKFKLEAPDADELPRAEFDPGQDTYQHPPKDSSGQQVDVSPTSQRLQLLEPFDKWDGRDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINVENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPADYNKIHPVDKLTIKGLKDFAPGKPLTCIIKHPNGTQETILLNHTFNETQIEWFRAGSALNRMKELQK 91EB21B3923F16C5 ncald_bovin Neurocalcin-delta NCALD protein protein bovin Bos taurus (Bovine) Bovine MGKQNSKLRPEVMQDLLESTDFTEHEIQEWYKGFLRDCPSGHLSMEEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFIIALSVTSRGKLEQKLKWAFSMYDLDGNGYISKAEMLEIVQAIYKMVSSVMKMPEDESTPEKRTEKIFRQMDTNRDGKLSLEEFIRGAKSDPSIVRLLQCDPSSAGQF 20DD8B6FF47C4CBF arf1_bovin ADP-ribosylation factor 1 ARF1 protein protein bovin Bos taurus (Bovine) Bovine MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK AAC773D4A60186B6 hpca_rat Neuron-specific calcium-binding protein hippocalcin Hpca P23K protein protein rat Rattus norvegicus (Rat) Rat MGKQNSKLRPEMLQDLRENTEFSELELQEWYKGFLKDCPTGILNVDEFKKIYANFFPYGDASKFAEHVFRTFDTNSDGTIDFREFIIALSVTSRGRLEQKLMWAFSMYDLDGNGYISREEMLEIVQAIYKMVSSVMKMPEDESTPEKRTEKIFRQMDTNNDGKLSLEEFIRGAKSDPSIVRLLQCDPSSASQF 3DBEA176AC945DB1 gfap_bovin Glial fibrillary acidic protein GFAP protein protein bovin Bos taurus (Bovine) Bovine MERRRVTSATRRSYVSSSEMVVGGRRLGPGTRLSLARMPPPLPARVDFSLAGALNSGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLGTLRQKLQDETNQRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLAQQQVHVEMDVAKPDLTAALREIRTQYEAVASSNMHEAEEWYRSKFADLNDAAARNAELLRQAKHEANDYRRQLQALTCDLESLRGTNESLERQMREQEERHAREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM 8DCCDF3E848BAD05 atpa_bovin ATP synthase subunit alpha, mitochondrial ATP5F1A ATP synthase F1 subunit alpha ATP5A1 ATP5A2 protein protein bovin Bos taurus (Bovine) Bovine MLSVRVAAAVARALPRRAGLVSKNALGSSFIAARNLHASNSRLQKTGTAEVSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVGEELLGRVVDALGNAIDGKGPIGSKARRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIGRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGTDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDAFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREVAAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVRGYLDKLEPSKITKFENAFLSHVISQHQALLSKIRTDGKISEESDAKLKEIVTNFLAGFEA DIP protein P19483 original sequence version: 148 DIP protein P19483 original sequence version: 91 DIP protein P19483 original sequence version: 90 DIP protein P19483 original sequence version: 106 DIP protein P19483 original sequence version: 124 DIP protein P19483 original sequence version: 132 188E9531B3B815E0 cn37_bovin 2',3'-cyclic-nucleotide 3'-phosphodiesterase CNP protein protein bovin Bos taurus (Bovine) Bovine MSSSGAKDKPELQFPFLQDEETVATLQECKTLFILRGLPGSGKSTLARFIVDKYRDGTKMVSADSYKITPGARGSFSEEYKQLDEDLAACCRRDFRVLVLDDTNHERERLEQLFELADQYQYQVVLVEPKTAWRLDCAQLKEKNQWQLSADDLKKLKPGLEKDFLPLYFGWFLTKKSSAALWKTGQTFLEELGNHKAFKKELRHFVSGDEPREKIELVTYFGKRPPGVLHCTTKFCDYGKAAGAEEYAQQDVVKKSYCKAFTLTISALFVTPKTTGARVELSEQQLALWPNDVDKLSPSDNLPRGSRAHITLGCAGDVEAVQTGIDLLEIVRQEKGGSRGEEVGELSRGKLYSLGSGRWMLSLAKKMEVRAIFTGYYGKGKAVPIRSGRKGGSFQSCTII 3448FC367D647CF8 ncs1-aco2 1 8 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine 12 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine 9 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine 14 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine 5 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine 10 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine 15 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine 6 unspecified role unspecified role bait bait n terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) association association All protein interactions were calcium dependent. cabp1-tcp1 1 16 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine 3 unspecified role unspecified role bait bait c terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association This interaction was shown to be calcium independent. ncs1-arf1 2 12 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine 6 unspecified role unspecified role bait bait n terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association ncs1-pde 2 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait n terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association ncald-pdea1 1 9 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine 11 unspecified role unspecified role bait bait n terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine association association hpca-pdea1 1 4 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine 8 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine 7 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine 13 unspecified role unspecified role bait bait n terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association hpac-pde-1 2 13 unspecified role unspecified role bait bait n terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey physical association physical association ncald-pde-2 2 11 unspecified role unspecified role bait bait bovin Bos taurus (Bovine) Bovine 8 unspecified role unspecified role prey prey physical association physical association