IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A higher plant has three different types of RPA heterotrimeric complex. A higher plant has three different types of RPA heterotrimeric complex. J. Biochem. (0021-924X) 2006 Ishibashi T., Kimura S., Sakaguchi K. Directed yeast two hybrid Figure 2 (A). kengo@rs.noda.tus.ac.jp yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Ishibashi T., Kimura S., Sakaguchi K. Directed yeast two hybrid Figure 2 (A). kengo@rs.noda.tus.ac.jp J. Biochem. (0021-924X) 2006 A higher plant has three different types of RPA heterotrimeric complex. A higher plant has three different types of RPA heterotrimeric complex. J. Biochem. (0021-924X) 2006 Ishibashi T., Kimura S., Sakaguchi K. Directed yeast two hybrid Figure 2 (A). kengo@rs.noda.tus.ac.jp in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Ishibashi T., Kimura S., Sakaguchi K. Directed yeast two hybrid Figure 2 (A). kengo@rs.noda.tus.ac.jp J. Biochem. (0021-924X) 2006 A higher plant has three different types of RPA heterotrimeric complex. A higher plant has three different types of RPA heterotrimeric complex. J. Biochem. (0021-924X) 2006 Ishibashi T., Kimura S., Sakaguchi K. Directed yeast two hybrid Figure 2 (A). kengo@rs.noda.tus.ac.jp orysa Oryza sativa (Rice) Rice anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ishibashi T., Kimura S., Sakaguchi K. Directed yeast two hybrid Figure 2 (A). kengo@rs.noda.tus.ac.jp J. Biochem. (0021-924X) 2006 rfa3_orysj Replication protein A 14 kDa subunit RPA3 RPA14 Replication factor A protein 3 Replication protein A 3 OsJ_01130 P0705D01.4 LOC_Os01g14980 Os01g0253600 protein protein orysj Oryza sativa subsp. japonica (Rice) Rice MDTSGPAAFVNGEILKMFVGRRVRTVVQAQREEGGLLIGQSTDGHQLTIKGASGAPMSHYVEIIGIAEPNQAIRAEVCTDFGENFDPAPFNGLCKLANGQMKDLFL 24F4A63FFB54A141 rfa1c_orysj Replication protein A 70 kDa DNA-binding subunit C RPA1C RPA70C Replication factor A protein 1C Replication protein A 1C OsJ_16860 P0016H04.11 LOC_Os05g02040 Os05g0111000 protein protein orysj Oryza sativa subsp. japonica (Rice) Rice MEPQLTPGAVQAIAEHPDGTGTIQPVLQVVDVRPVTTKNAPPTPKPAERFRMMLSDGVNTQQSMLATALNPLVKDATLRPGTVVQLTDFMCNTIQGKRIIIVVKLDVLQNDCIVIGNPKHYEPKSLTKEQDPNLQASVAQTNNGTYSGGASMLGPSVAPRAEQAASNSSYGGPYNSAQGMLGSSIGRTVEPGPANVSAVGSYGAISAQNTTNANMMQPTSQLNIMNANTMQPTSQLNTMNANTMQPTSQLSSLNPNQNQRFAAPASGGVFGPPGNAYGQPSRPSYQQPPPVYMNRGPASRNDSATRIIPITALNPYQPKWTIKARVTAKSDIRHWSNARSSGTVFSFDLLDAQGGEIRAQCWKESADKFFGQIEVGRVYLISRGSLKPAQKKYNTLNHDYEITLDIGLSTVEVCSDDDNSIPRLQYNFRQISELENMANETIVDLLGVVTSVSPSATIMRKIGTETRKRSIQLKDLSGRSIEVTLWGNFCDAEGQQLQLQCDSGSNPIIAFKGARVGDFNGKSVSTIGSTQLIINPDFPEVERLRQWYMTEGKTAPCISLSREMLNMGRTDARKTIAQIKDENLGRLEKPDWITVKAAISHVTTESFCYPACPKLLPVGRQCNKKAINNGDGMWHCDRCDESFQNPEYRYMLRFQIQDHTGSTYASAFDEAGEQIFGRKAGELFSIRNVDQDDAQFAEIIEGVRWHLYLFKLKVKEETYNDEQSLKCTAVKVEKLDPSKESNVLLGAIDNLLLDPKGQSDLAPNAGFTDPVGGHGAPTSSNAYAMNTGGVNQFGQQASISAGMSTPLAATRNLQTCSICGANGHSAQICHVGADMDMQETSAGGSSMGNYNSIAGNGSSECYKCKQPGHYARDCPGQSTGGLECFKCKQPGHFSRDCPVQSTGGSECFKCKQPGHFARDCPGQSTGAQHQTYGNNVAASRGYNRQSFVGGY FFD5D5B40CD01616 rfa2b_orysj Replication protein A 32 kDa subunit B RPA2B OsRPA32-2 RPA32B Replication factor A protein 2B Replication protein A 2B OJ1643_A10.19 LOC_Os02g42230 Os02g0633400 protein protein orysj Oryza sativa subsp. japonica (Rice) Rice MYGVGVGGGGGGNYDGGGGNASSLFGGGGFMPSQATNAAEGTSGGGGGFPKSRNAQALLPLTVKQIMDASQTNDDKSNFAVNGMEVSTVRLVGRMLNKLDRVTDVSFTLDDGTGRVPVNRWENDSTDTKEMADIQNGDYVIVNGGLKGFQGKRQVVAYSVRRITNFNDVTHHFLHCVHVHLELTRPKSQVNANTATGTPNQTMPRDSMAYNQSPLTNQASTFSAPQNTGTGTNMIDLVLNVFHDPAVMNDDHGVGVDYVSRRLNLPEETVGKIIIDQVDLGHLYATIDDHHYKSTMNG 89E420E9C5AA0AA3 rfa2a_orysj Replication protein A 32 kDa subunit A RPA2A OsRPA32-1 RPA32A Replication factor A protein 2A Replication protein A 2A OJ1124_D06.4 OsJ_08977 LOC_Os02g58220 Os02g0829100 protein protein orysj Oryza sativa subsp. japonica (Rice) Rice MMSFSQPDAFSPSQFTSSQNAAADSTTPSKSRGASSTMPLTVKQISEAQQSGITGEKGAPFVVDGVETANVRLVGLVSGKTERNTDVSFTIDDGTGRLDFIRWVNDGADSAETAAVQNGMYVSVIGSLKGLQERKRATAFAIRPVTDYNEVTLHFIQCVRMHLENTKSQIGSPAKTYSAMGSSSSNGFSEMTTPTSVKSNPAPVLSVTNGSKTDLNTEVLNVFREPANVESEHGVHIDEIVKRFRLPEAKIKVAIDYLADIGHIYSTIDESHYKSAFNE EA953E8D120A8913 rfa2c_orysj Replication protein A 32 kDa subunit C RPA2C OsRPA32-3 RPA32C Replication factor A protein 2C Replication protein A 2C P0550B04.15 LOC_Os06g47830 Os06g0693300 protein protein orysj Oryza sativa subsp. japonica (Rice) Rice MAAAASYFSGTALMPSQRSGAPAPEYSAAGTGAAAAPSPSKPRDPRFSGCVPATVLHISRSFAAALAADGGGDPVFSIDGVETTNVRVLGRVVSVVSRDTDVCFTLDDSTGKIPLVRWITDQSDTRDTSYIQEGVYVKVQVNLMGFQAKKQGLARSIRPINNFNEVVLHFIECMHVHLESVQSKMQRQLPPSVQTNEYTHVPSSGGVRDYQVHFTPQVNQGLPPAVQTNTSTYVPLLGGVRDHQAHFAQVNQGQFSPAVQANTSTHLPFSGGVGEHQIHFTPKVNQGQFPPSVQTNTSAHVPYSGGFREHQVHFTPPVNQGQFPPAVQTNLYNHAASSGGVREQVHLTQANQFSAYSSTGGLQHDPQRMVLEALQQPDILALEHGAHVDELVRRTGMPKANIMGVVKHLAAAGFVYWTIDDNHVKSMCNG DFD46172046D146F rfa1a_orysj Replication protein A 70 kDa DNA-binding subunit A RPA1A RPA70A Replication factor A protein 1A Replication protein A 1A OJ1534_E09.2 OSJNBb0013K01.36 OsJ_08582 LOC_Os02g53680 Os02g0776800 protein protein orysj Oryza sativa subsp. japonica (Rice) Rice MAMARLTPNGVAAALAGDTNLKPVLQIVELRGVQVNGAGVTRGERFRAVVSDGTAASSALFAAQLSDHARSGALRRGSIVQLSEYVINEVGPRRIIVILNLEVLVSECEIIGNPTALSETGSPIPNPTRVEQFNGAPQYGLMAGNSSNTTTKPSDNVPLFQNSMAGNSSNFATRPSDKVPVFQPTVQPSYRPAPNYKNHGAIMKNEAPARIIPISALNPYQGRWAIKARVTAKGDIRRYHNAKGDGKVFSFDLLDSDGGEIRVTCFNALLDRFYEVVEVGKVYVVSRGNLRPAQKNYNHLNNEWEILLENGSTVDLCPDENSSIPTQRFDFRPINEIEDAQNNAILDIIGVVTSVNPCTTIQRKNGMETQKRTMNLKDMSGRSVEVTMWGDFCNREGSQLQGMVERGIFPVLAVKAGKVSDFSGKSVGTISSTQLFINPDSAEAHSLRQWFDSGGRDASTQSISRDITPGASRNEIRKTVAQIKDEGLGMGDKPDWITVKATVIFFKNESFFYTACPNMIGDRQCNKKVTKSTNGNWTCDKCDREFEECDYRYLLQFQIQDHSGTAWVTAFQEAGQELLGCSATELNALKEREDPRFADTMLNCLFQEYLLRLKVKEESYGDERKVKNTAVKVEKVDPSGESKFLLDLISKSSALH B4C4492EAFEC4556 osrpa32-osrpa14 1 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association q6h7j5-osrpa14 1 4 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association q5z8l1-osrpa14 1 4 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association q5z8l1-q65xv7 1 8 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Very weak interactions between OsRAP32-3 and OsRAP70a and OsRAP70b were detected. q9avm2-osrpa14 1 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Weak interactions between OsRAP32-1 and OsRAP70b or OsRAP70c were detected. q6h7j5-osrpa70a 1 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Weak interactions between Q6H7J5 and Q7Y069 or Q65XV7. q6h7j5-q9avl8-1 2 6 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey physical association physical association A weak interaction between OsRAP32-2 and OsRAP70c was detected. q5z8l1-q65xv7-1 2 5 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association q9avl8-q6h7j5 2 9 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey physical association physical association q9avm2-q7y069-1 2 7 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey physical association physical association A weak interaction between OsRAP32-1 and OsRAP70c was detected. q65xv7-q5z8l1 2 5 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey physical association physical association q7y069-q9avm2 2 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association A weak interaction between OsRAP70b and OsRAP32-2 was detected. q9avm2-p0705d01_4-1 3 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association q6h7j5-p0705d01_4-2 3 4 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association q5z8l1-p0705d01_4-3 3 8 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association q6h7j5-q9avl8-2 3 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association q5z8l1-q65xv7-2 3 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association