IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Interactions of the Escherichia coli hydrogenase biosynthetic proteins: HybG complex formation. Interactions of the Escherichia coli hydrogenase biosynthetic proteins: HybG complex formation. 2006 Butland G., Zhang JW., Yang W., Sheung A., Wong P., Greenblatt JF., Emili A., Zamble DB. A c-terminal sequence peptide affinity (SPA), a DNA cassette encoding two affinity purification tags was introduced into the E. coli chromosome appended to one of the genes of the hydrogenase accessory proteins. fig 1, fig 2, fig 3, fig 4 dzamble@chem.utoronto.ca ecoli Escherichia coli (strain K12) pull down pull down fingerprinting peptide massfingerprinting fingerprinting Butland G., Zhang JW., Yang W., Sheung A., Wong P., Greenblatt JF., Emili A., Zamble DB. A c-terminal sequence peptide affinity (SPA), a DNA cassette encoding two affinity purification tags was introduced into the E. coli chromosome appended to one of the genes of the hydrogenase accessory proteins. fig 1, fig 2, fig 3, fig 4 dzamble@chem.utoronto.ca 2006 hyfg_ecoli Hydrogenase-4 component G hyfG b2487 JW2472 protein protein ecoli Escherichia coli (strain K12) MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINLLPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRLRPEPTTDSETYPFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK DIP protein P77329 original sequence version: null A5D410E6E74C05CD hyce_ecoli Formate hydrogenlyase subunit 5 Hydrogenase-3 component E hycE hevE b2721 JW2691 protein protein ecoli Escherichia coli (strain K12) MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPEVVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYPSVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPAPTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK DIP protein P16431 original sequence version: null 38A2BCCB01B27463 mbht_ecoli Hydrogenase-2 small chain yghV Membrane-bound hydrogenase 2 small subunit NiFe hydrogenase hybO b2997 JW2965 protein protein ecoli Escherichia coli (strain K12) MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVTNPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENKHNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAWGGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKNRPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQFCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSAGAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE C5821096BBAF9C66 C5821096BBAF9C66 hybe_ecoli Hydrogenase-2 operon protein HybE hybE b2992 JW2960 protein protein ecoli Escherichia coli (strain K12) MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSDFTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGVSQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGENA CFDAEA3D47BE9516 CFDAEA3D47BE9516 hypd_ecoli Hydrogenase maturation factor HypD hypD Hydrogenase isoenzymes formation protein HypD JW2699 b2729 protein protein ecoli Escherichia coli (strain K12) MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGHTHAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRVPGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQAKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDFHRPLVVAGFEPLDLLQGVVMLVQQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCVNGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPHQCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA DIP protein P24192 original sequence version: 70 DIP protein P24192 original sequence version: null 0E4186081FAA4C54 mbhl_ecoli Hydrogenase-1 large chain Membrane-bound hydrogenase 1 large subunit NiFe hydrogenase hyaB b0973 JW0955 protein protein ecoli Escherichia coli (strain K12) MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVITNAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDNANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSPGYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVKIHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMIPDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNVLPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLGRILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAPRGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEILRTLHSFDPCLACSTHVLGDDGSELISVQVR DIP protein P0ACD8 original sequence version: 60 DIP protein P0ACD8 original sequence version: 81 659DD7EDE16D6342 mbhm_ecoli Hydrogenase-2 large chain hybC b2994 JW2962 Membrane-bound hydrogenase 2 large subunit NiFe hydrogenase protein protein ecoli Escherichia coli (strain K12) MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVASGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNLAVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRGKGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHSWYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRESTQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNIGKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKVL DIP protein P0ACE0 original sequence version: 38 A19E6F513390F29E hype_ecoli Carbamoyl dehydratase HypE hypE Hydrogenase maturation factor HypE JW2700 b2730 protein protein ecoli Escherichia coli (strain K12) MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLAQLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEEGLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAVDKLFINTAGMGAIPANIHWGAQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKALRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLVIAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPRIC DIP protein P24193 original sequence version: null 0811EE415F11AF6C hypb_ecoli Hydrogenase maturation factor HypB hypB Hydrogenase isoenzymes nickel incorporation protein HypB JW2697 b2727 protein protein ecoli Escherichia coli (strain K12) MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIKAPEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAARKQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQVNTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEGEDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEGMDQWLNWLETQRCA DIP protein P0AAN3 original sequence version: 30 5B3AEB59AA6915C3 q6q099_ecolx 60 kDa chaperonin mopA GroEL protein Protein Cpn60 groEL groL protein protein ecolx Escherichia coli MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTVAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM 03447FE9D1F1E86C hybg_ecoli Hydrogenase maturation factor HybG hybG Hydrogenase-2 operon protein HybG JW2958 b2990 protein protein ecoli Escherichia coli (strain K12) MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADLLGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA DIP protein P0AAM7 original sequence version: 32 8E9736BFC4EFD626 hypc_ecoli Hydrogenase maturation factor HypC hypC Chaperone-type protein HypC Hydrogenase accessory chaperone HypC JW2698 b2728 protein protein ecoli Escherichia coli (strain K12) MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRVGQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK DIP protein P0AAM3 original sequence version: 32 FBE252F1875C7879 slyd_ecoli FKBP-type peptidyl-prolyl cis-trans isomerase SlyD slyD b3349 Metallochaperone SlyD JW3311 Histidine-rich protein WHP Rotamase Sensitivity to lysis protein D protein protein ecoli Escherichia coli (strain K12) MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISGLETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQGPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHHDHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH DIP protein P0A9K9 original sequence version: 42 46DCC7C7ECD61DBA hybe-hybo-1 1 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait spa tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association P0AAN1 endogenous hypb-slyd-1 1 14 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait spa tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association P0AAN3 endogenous hypb-slyd-2 1 10 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait spa tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association P0A9K9 endogenous hybg-hypd 1 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait spa tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association HypD and HypE need to form a complex prior to association with HybG. This was demonstrated by pull-down analysis in KO mutants either for HypD or for HypE. P0AAM7 endogenous Biosynthesis of the Nickel- and Iron-containing catalytic center of [NiFe] hydrogenase enzymes located on the larger subunit requires the activity of multiple accessory proteins. hypd-hypc-1 1 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait spa tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association P24192 endogenous Biosynthesis of the Nickel- and Iron-containing catalytic center of [NiFe] hydrogenase enzymes located in the larger subunit requires the activity of multiple accessory proteins. hypc-hypd-1 1 2 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait spa tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association P0AAM3 endogenous Biosynthesis of the Nickel- and Iron-containing catalytic center of [NiFe] hydrogenase enzymes located in the larger subunit requires the activity of multiple accesory proteins.