MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Yeast Trf5p is a nuclear poly(A) polymerase. Yeast Trf5p is a nuclear poly(A) polymerase. EMBO Rep. (1469-221X) 2006 imex curation Houseley J., Tollervey D. d.tollervey@ed.ac.uk 09-April-2009: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Houseley J., Tollervey D. d.tollervey@ed.ac.uk Accepted 2009-MAR-24 by ORCHARD. 09-April-2009: Contacted by JYOTI. EMBO Rep. (1469-221X) 2006 orchard Only protein-protein interactions imex curation Yeast Trf5p is a nuclear poly(A) polymerase. Yeast Trf5p is a nuclear poly(A) polymerase. EMBO Rep. (1469-221X) 2006 imex curation Houseley J., Tollervey D. d.tollervey@ed.ac.uk 09-April-2009: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast enzymatic study enzymatic study experimental particp experimental participant identification experimental particp Houseley J., Tollervey D. d.tollervey@ed.ac.uk Accepted 2009-MAR-24 by ORCHARD. 09-April-2009: Contacted by JYOTI. EMBO Rep. (1469-221X) 2006 orchard Only protein-protein interactions imex curation trf5_yeast Poly(A) RNA polymerase protein 1 TRF5 YNL299W N0440 Topoisomerase 1-related protein TRF5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTRLKAKYSPTKGKRKEDKHTKRMRKSSFTRTQKMLEVFNDNRSHFNKYESLAIDVEDDDTFGNLVLMENDKSDVDIPVIEEVTSSEDEQRAESSKRNNSLEDNQDFIAFSDSSEDETEQIKEDDDERSSFLLTDEHEVSKLTSQQSLNTESACNVEYPWIRNHCHSKQRRIADWLTSEIKDFVHYISPSKNEIKCRNRTIDKLRRAVKELWSDADLHVFGSFATDLYLPGSDIDCVVNSRNRDKEDRNYIYELARHLKNKGLAIRMEVIVKTRVPIIKFIEPQSQLHIDVSFERTNGLEAAKLIREWLRDSPGLRELVLIIKQFLHSRRLNNVHTGGLGGFTVICLVYSFLNMHPRIKSNDIDVLDNLGVLLIDFFELYGKNFGYDDVAISISDGYASYIPKSCWRTLEPSRSKFSLAIQDPGDPNNNISRGSFNMKDIKKAFAGAFELLVNKCWELNSATFKDRVGKSILGNVIKYRGQKRDFNDERDLVQNKAIIENERYHKRRTRIVQEDLFINDTEDLPVEEIYKLDEPAKKKQKAKKDKREGEIKKSAIPSPPPDFGVSRSKLKRKVKKTDQGSLLHQNNLSIDDLMGLSENDQESDQDQKGRDTPSGQDEKSPLETKTVDAQTRRDYWLSKGQAL 67D4CD75AEBAE1A4 air1_yeast Protein AIR1 Arginine methyltransferase-interacting RING finger protein 1 AIR1 YIL079C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTLLSEVESIDTLPYVKDTTPTGSDSSSFNKLLAPSIEDVDANPEELRTLRGQGRYFGITDYDSNGAIMEAEPKCNNCSQRGHLKRNCPHVICTYCGFMDDHYSQHCPKAIICTNCNANGHYKSQCPHKWKKVFCTLCNSKRHSRERCPSIWRSYLLKTKDANQGDFDFQTVFCYNCGNAGHFGDDCAERRSSRVPNTDGSAFCGDNLATKFKQHYFNQLKDYKREASQRQHFDNEHEFNLLDYEYNDDAYDLPGSRTYRDKMKWKGKVQSTRNKNSSNNRYESSNNRKKKSPFSAQNYKVTKNKRVQTHPLDFPRSSQNNRTNDYSSQFSYNRDDFPKGPKNKRGRSSSNKSQRNGRY 3CA920FA1204EC9C 3CA920FA1204EC9C polya_rna Homopolymeric adenosine rna ribonucleic acid RNA rna in vitro In vitro mtr4_yeast ATP-dependent RNA helicase DOB1 MTR4 DOB1 YJL050W J1158 mRNA transport regulator MTR4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDSTDLFDVFEETPVELPTDSNGEKNADTNVGDTPDHTQDKKHGLEEEKEEHEENNSENKKIKSNKSKTEDKNKKVVVPVLADSFEQEASREVDASKGLTNSETLQVEQDGKVRLSHQVRHQVALPPNYDYTPIAEHKRVNEARTYPFTLDPFQDTAISCIDRGESVLVSAHTSAGKTVVAEYAIAQSLKNKQRVIYTSPIKALSNQKYRELLAEFGDVGLMTGDITINPDAGCLVMTTEILRSMLYRGSEVMREVAWVIFDEVHYMRDKERGVVWEETIILLPDKVRYVFLSATIPNAMEFAEWICKIHSQPCHIVYTNFRPTPLQHYLFPAHGDGIYLVVDEKSTFREENFQKAMASISNQIGDDPNSTDSRGKKGQTYKGGSAKGDAKGDIYKIVKMIWKKKYNPVIVFSFSKRDCEELALKMSKLDFNSDDEKEALTKIFNNAIALLPETDRELPQIKHILPLLRRGIGIHHSGLLPILKEVIEILFQEGFLKVLFATETFSIGLNMPAKTVVFTSVRKWDGQQFRWVSGGEYIQMSGRAGRRGLDDRGIVIMMIDEKMEPQVAKGMVKGQADRLDSAFHLGYNMILNLMRVEGISPEFMLEHSFFQFQNVISVPVMEKKLAELKKDFDGIEVEDEENVKEYHEIEQAIKGYREDVRQVVTHPANALSFLQPGRLVEISVNGKDNYGWGAVVDFAKRINKRNPSAVYTDHESYIVNVVVNTMYIDSPVNLLKPFNPTLPEGIRPAEEGEKSICAVIPITLDSIKSIGNLRLYMPKDIRASGQKETVGKSLREVNRRFPDGIPVLDPVKNMKIEDEDFLKLMKKIDVLNTKLSSNPLTNSMRLEELYGKYSRKHDLHEDMKQLKRKISESQAVIQLDDLRRRKRVLRRLGFCTPNDIIELKGRVACEISSGDELLLTELIFNGNFNELKPEQAAALLSCFAFQERCKEAPRLKPELAEPLKAMREIAAKIAKIMKDSKIEVVEKDYVESFRHELMEVVYEWCRGATFTQICKMTDVYEGSLIRMFKRLEELVKELVDVANTIGNSSLKEKMEAVLKLIHRDIVSAGSLYL 1FA9EAADC546F669 1FA9EAADC546F669 trf5-mtr4-1 1 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 4E Both Mtr4p and Air1p were identified by mass spectrometry as bands that showed approximate stoichiometry with Trf5-TAP. Rpl2B and Ccp1 were also identified but are abundant proteins that are likely to be contaminants. trf5-polya 2 3 enzyme enzyme neutral component neutral component c-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range P48561:p.[Asp233Ala;Asp235Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain D A Wed May 02 17:37:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp233ala,asp235ala' Tue Jun 30 18:44:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp233Ala;Asp235Ala]' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 5 enzyme target enzyme target substrate substrate neutral component neutral component enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction Fig 5 Trf5�TAP reproducibly showed poly(A) polymerase activity.