IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A protein network for phospholipid synthesis uncovered by a variant of the tandem affinity purification method in Escherichia coli. A protein network for phospholipid synthesis uncovered by a variant of the tandem affinity purification method in Escherichia coli. Proteomics (1615-9853) 2006 imex curation Gully D., Bouveret E. Tagged proteins were over-expressed using an arabinose inducible expression system in E.coli W3110. Figure 1. Figure 5 (A). bouveret@ibsm.cnrs-mrs.fr 1460..1470 1460..1480 2006-09-05: Only interaction involving solely Proteins were exported. Only protein-protein interactions 12 ecoli Escherichia coli (strain K12) adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Gully D., Bouveret E. Tagged proteins were over-expressed using an arabinose inducible expression system in E.coli W3110. Figure 1. Figure 5 (A). bouveret@ibsm.cnrs-mrs.fr Annotation to indicate that this entry has been checked and accepted by a senior curator. Proteomics (1615-9853) 2006 1460..1470 1460..1480 2006-09-05: Only interaction involving solely Proteins were exported. Only protein-protein interactions 12 imex curation A protein network for phospholipid synthesis uncovered by a variant of the tandem affinity purification method in Escherichia coli. A protein network for phospholipid synthesis uncovered by a variant of the tandem affinity purification method in Escherichia coli. Proteomics (1615-9853) 2006 imex curation Gully D., Bouveret E. Tagged proteins were over-expressed using an arabinose inducible expression system in E.coli W3110. Figure 1. Figure 5 (A). bouveret@ibsm.cnrs-mrs.fr 1460..1470 1460..1480 2006-09-05: Only interaction involving solely Proteins were exported. Only protein-protein interactions 12 ecow3 Escherichia coli (strain K12 / W3110 / ATCC 27325 / DSM 5911) affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Gully D., Bouveret E. Tagged proteins were over-expressed using an arabinose inducible expression system in E.coli W3110. Figure 1. Figure 5 (A). bouveret@ibsm.cnrs-mrs.fr Annotation to indicate that this entry has been checked and accepted by a senior curator. Proteomics (1615-9853) 2006 1460..1470 1460..1480 2006-09-05: Only interaction involving solely Proteins were exported. Only protein-protein interactions 12 imex curation A protein network for phospholipid synthesis uncovered by a variant of the tandem affinity purification method in Escherichia coli. A protein network for phospholipid synthesis uncovered by a variant of the tandem affinity purification method in Escherichia coli. Proteomics (1615-9853) 2006 imex curation Gully D., Bouveret E. Tagged proteins were over-expressed using an arabinose inducible expression system in E.coli W3110. Figure 1. Figure 5 (A). bouveret@ibsm.cnrs-mrs.fr 1460..1470 1460..1480 2006-09-05: Only interaction involving solely Proteins were exported. Only protein-protein interactions 12 ecow3 Escherichia coli (strain K12 / W3110 / ATCC 27325 / DSM 5911) tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Gully D., Bouveret E. Tagged proteins were over-expressed using an arabinose inducible expression system in E.coli W3110. Figure 1. Figure 5 (A). bouveret@ibsm.cnrs-mrs.fr Annotation to indicate that this entry has been checked and accepted by a senior curator. Proteomics (1615-9853) 2006 1460..1470 1460..1480 2006-09-05: Only interaction involving solely Proteins were exported. Only protein-protein interactions 12 imex curation fabb_ecoli 3-oxoacyl-[acyl-carrier-protein] synthase 1 fabB fabC b2323 JW2320 3-oxoacyl-[acyl-carrier-protein] synthase I Beta-ketoacyl-ACP synthase I protein protein ecoli Escherichia coli (strain K12) MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGMRSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLIAGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSISSACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKASRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEGAVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHSLGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGFGGTNATLVMRKLKD 489D8BAD23E78113 489D8BAD23E78113 plsb_ecoli Glycerol-3-phosphate acyltransferase plsB b4041 JW4001 protein protein ecoli Escherichia coli (strain K12) MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYVLPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESIKLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLGRDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFNKLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWNRLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTGRLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLPQMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNIAADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDSTVPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMAAIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQDDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLSVLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLTIESATQGEG D6FC6892981D7EFE D6FC6892981D7EFE ybgc_ecoli Acyl-CoA thioester hydrolase YbgC ybgC b0736 JW0726 protein protein ecoli Escherichia coli (strain K12) MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHHFSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENTLLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ C44582B6EC3BE989 C44582B6EC3BE989 iscs_ecoli Cysteine desulfurase IscS iscS b2530 JW2514 yfhO yzzO ThiI transpersulfidase NifS protein homolog TusA transpersulfidase nuvC protein protein ecoli Escherichia coli (strain K12) MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFGWQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKTEHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVVQDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYVRRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNRLWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQGVDLNSIEWAHH 1E4F2E6F9CD266B0 1E4F2E6F9CD266B0 mukb_ecoli Chromosome partition protein MukB mukB b0924 JW0907 Structural maintenance of chromosome-related protein protein protein ecoli Escherichia coli (strain K12) MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTMAAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVVGVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAMEGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLRDYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMRHANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAASDHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQQENEARAEAAELEVDELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADCAAEWLETFQAKELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQRHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARIASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTSSQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFGGVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGDPQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLSERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHENDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQFGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSYSDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDTKKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAEMDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDLRSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIRTDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGLQNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLNPQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSILVMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPENISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGTDEAPSQAS DIP protein P22523 original sequence version: 108 DIP protein P22523 original sequence version: 122 DIP protein P22523 original sequence version: null 38C7874BEB78D6D6 spot_ecoli Bifunctional (p)ppGpp synthase/hydrolase SpoT spoT b3650 JW3625 protein protein ecoli Escherichia coli (strain K12) MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIARETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRVLGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMGVAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENIQRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQEFITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN A85F70F57D082EE8 A85F70F57D082EE8 seca_ecoli Protein translocase subunit SecA secA azi pea prlD b0098 JW0096 protein protein ecoli Escherichia coli (strain K12) MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELKGKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEMRTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPGMPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDEARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTERGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVIIVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTGTADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQPVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAGRGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRRIDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVTKAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIREDVFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERILAQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKDPKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQLSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ DIP protein P10408 original sequence version: 150 DIP protein P10408 original sequence version: 130 DIP protein P10408 original sequence version: 105 DIP protein P10408 original sequence version: 107 DIP protein P10408 original sequence version: 125 DIP protein P10408 original sequence version: 140 DIP protein P10408 original sequence version: null 683D66DD4305DBC2 fabf_ecoli 3-oxoacyl-[acyl-carrier-protein] synthase 2 fabF fabJ b1095 3-oxoacyl-[acyl-carrier-protein] synthase II Beta-ketoacyl-ACP synthase II JW1081 protein protein ecoli Escherichia coli (strain K12) MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTSAYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIGAAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISIATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQAASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPENGAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSSTKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGMEYTLCNSFGFGGTNGSLIFKKI 5F60DB1F986B2EE5 5F60DB1F986B2EE5 plsx_ecoli Phosphate acyltransferase plsX b1090 JW5156 Acyl-ACP phosphotransacylase Acyl-[acyl-carrier-protein]--phosphate acyltransferase Phosphate-acyl-ACP acyltransferase protein protein ecoli Escherichia coli (strain K12) MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNSDAITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQACVSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQFAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEANELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKSLTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAARLESVYPAGFELLDGGKSGTLR 89C6C64C57184DF4 acp_ecoli Acyl carrier protein acpP b1094 JW1080 Cytosolic-activating factor Fatty acid synthase acyl carrier protein protein protein ecoli Escherichia coli (strain K12) MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELVMALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA DIP protein P0A6A8 original sequence version: 43 DIP protein P0A6A8 original sequence version: 56 DIP protein P0A6A8 original sequence version: 86 41FCFC39D45BFEA1 espp_ecolx Serine protease EspP espP pssA protein protein ecolx Escherichia coli MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCFLGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPFPDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVSRLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRITPFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKKWVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTGTGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDTRNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITSGDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKKSILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQGHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDLSQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNITGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIENGADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKNKASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSASATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNLSESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGFSDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMGDLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTDSSASADVFSGKTKSVGAGLYASAMVDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYSTHSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSVGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF F84D24086DE77C24 acp-ygbc 2 6 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait region calmodulin binding protein tagged tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P0A6A8:p.Ser37Cys mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S C Wed May 02 16:20:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser37cys' Tue Jun 30 17:54:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser37Cys' physical association physical association ygbc-acp 2 6 unspecified role unspecified role bait bait region calmodulin binding protein tagged tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role prey prey physical association physical association acp-ygbc-1 3 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait region Protein A tagged tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association ygbc-apc-plsb 3 14 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait region Protein A tagged tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region calmodulin binding protein tagged tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Both YbgC and ACP act as baits, in first stage of TAP ACP acts as a bait, then YbgC a second round of affinity chromtography is applied with YbgC acting as a as a bait. ygbc-sec1 3 10 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association ygbc-plsx 3 14 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region Calmodulin binding protein tagged tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P0A8Z3:p.[Tyr14Asn;Tyr24Asn] mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y N certain certain sequence position certain certain certain sequence position certain Y N Wed May 02 19:05:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr14asn,tyr24asn' Tue Jun 30 18:58:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr14Asn;Tyr24Asn]' association association The mutations Y14N and Y24N in P0A8Z6 increased interaction with PlSb and espp and apppeared to cause interaction with PlsX. acp-plsb 1 5 unspecified role unspecified role neutral component neutral component region T25 adenylate cyclase tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-861859]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 808-808, sequence length 807 [EBI-861859]261-808 13 unspecified role unspecified role neutral component neutral component region T18 adenylate cyclase tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association The proteins were labelled neutral component as no clear bait + prey relationship was clear in this instance. ybgc-plsb 1 6 unspecified role unspecified role neutral component neutral component region T18 adenylate cyclase tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component region T25 adenylate cyclase tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-861855]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 808-808, sequence length 807 [EBI-861855]261-808 physical association physical association The proteins were labelled neutral component as no clear bait + prey relationship was clear in this instance. acp-pssa 2 14 unspecified role unspecified role bait bait region Calmodulin binding protein tagged tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role prey prey region Protein A tagged tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association ygbc-pssa-1 2 14 unspecified role unspecified role bait bait region Calmodulin binding protein tagged tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region Protein A tagged tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association plsb-plsb 1 5 unspecified role unspecified role neutral component neutral component region T25 adenylate cyclase tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component region T18 adenylate cyclase tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Neutral component term used for interactors as bait and prey relationship not clear. plsb-pssa 1 5 unspecified role unspecified role neutral component neutral component region T25 adenylate cyclase tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role neutral component neutral component region T18 adenylate cyclase tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Neutral component term used for interactors as bait and prey relationship not clear.