IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Retroviral restriction factor TRIM5alpha is a trimer. Retroviral restriction factor TRIM5alpha is a trimer. J. Virol. (0022-538X) 2005 Mische CC., Javanbakht H., Song B., Diaz-Griffero F., Stremlau M., Strack B., Si Z., Sodroski J. HEK-293T cells transiently expressing the TRIM5-alpha variants were lysed and cross-linked with different concentrations (mM) of glutaraldehyde and ethylene glycolbis(succinimidylsuccinate). This was followed by anti-tag CoIP. 2 joseph_sodroski@dfci.harvard.edu human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Mische CC., Javanbakht H., Song B., Diaz-Griffero F., Stremlau M., Strack B., Si Z., Sodroski J. HEK-293T cells transiently expressing the TRIM5-alpha variants were lysed and cross-linked with different concentrations (mM) of glutaraldehyde and ethylene glycolbis(succinimidylsuccinate). This was followed by anti-tag CoIP. 2 joseph_sodroski@dfci.harvard.edu Accepted 2006-MAY-10 by ORCHARD. J. Virol. (0022-538X) 2005 Retroviral restriction factor TRIM5alpha is a trimer. Retroviral restriction factor TRIM5alpha is a trimer. J. Virol. (0022-538X) 2005 Mische CC., Javanbakht H., Song B., Diaz-Griffero F., Stremlau M., Strack B., Si Z., Sodroski J. HEK-293T cells transiently expressing the TRIM5-alpha variants were lysed and cross-linked with different concentrations (mM) of glutaraldehyde and ethylene glycolbis(succinimidylsuccinate). This was followed by anti-tag CoIP. 2 joseph_sodroski@dfci.harvard.edu human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Mische CC., Javanbakht H., Song B., Diaz-Griffero F., Stremlau M., Strack B., Si Z., Sodroski J. HEK-293T cells transiently expressing the TRIM5-alpha variants were lysed and cross-linked with different concentrations (mM) of glutaraldehyde and ethylene glycolbis(succinimidylsuccinate). This was followed by anti-tag CoIP. 2 joseph_sodroski@dfci.harvard.edu Accepted 2006-MAY-10 by ORCHARD. J. Virol. (0022-538X) 2005 trim5_chlae Tripartite motif-containing protein 5 TRIM5 TRIM5alpha RING-type E3 ubiquitin transferase TRIM5 protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey MASGILLNVKEEVTCPICLELLTEPLSLPCGHSFCQACITANHKESMLYKEEERSCPVCRISYQPENIQPNRHVANIVEKLREVKLSPEEGQKVDHCARHGEKLLLFCQEDSKVICWLCERSQEHRGHHTFLMEEVAQEYHVKLQTALEMLRQKQQEAEKLEADIREEKASWKIQIDYDKTNVSADFEQLREILDWEESNELQNLEKEEEDILKSLTKSETEMVQQTQYMRELISDLEHRLQGSMMELLQGVDGIIKRVENMTLKKPKTFHKNQRRVFRAPDLKGMLDMFRELTDVRRYWVDVTLAPNNISHAVIAEDKRQVSYQNPQIMYQAPGSSFGSLTNFNYCTGVLGSQSITSRKLTNFNYCTGVLGSQSITSGKHYWEVDVSKKSAWILGVCAGFQPDATYNIEQNENYQPKYGYWVIGLQEGDKYSVFQDSSSHTPFAPFIVPLSVIICPDRVGVFVDYEACTVSFFNITNHGFLIYKFSQCSFSKPVFPYLNPRKCTVPMTLCSPSS 4C81FB3BB63ED581 q2y080_aotaz Peptidyl-prolyl cis-trans isomerase protein protein aotaz Aotus azarai (Southern owl monkey) Aotus azarae MVNPTVFFDIAVDGEPLGRVSFELFADKLPKTAENFRALSTGEKGFGYKGSCFHRIIPGFMCQGGDFTPHNGTGGKSIYGVKFDDENFILKHTGPGILSMANAGPNTNGSQFFICTAKTEWLDGKHVVFGKVKEGMNVVEAMERFGCRYGKTSKKITIADCGQL 67C8DCFFCDEC596A q0pf16-2 Tripartite motif-containing protein 5 TRIM5 TRIM5alpha RING-type E3 ubiquitin transferase TRIM5 protein protein macmu Macaca mulatta (Rhesus macaque) Rhesus macaque MASGILLNVKEEVTCPICLELLTEPLSLHCGHSFCQACITANHKKSMLYKEGERSCPVCRISYQPENIQPNRHVANIVEKLREVKLSPEEGQKVDHCARHGEKLLLFCQEDSKVICWLCERSQEHRGHHTFLMEEVAQEYHVKLQTALEMLRQKQQEAEKLEADIREEKASWKIQIDYDKTNVSADFEQLREILDWEESNELQNLEKEEEDILKSLTKSETEMVQQTQYMRELISELEHRLQGSMMDLLQGVDGIIKRIENMTLKKPKTFHKNQRRVFRAPDLKGMLDMFRELTDARRYWGKEKSHYHQPPCGLSLLLSLSFRILYSLLGLVF No experimental confirmation available. 33D4B364F2DCB7EC trim5_macmu Tripartite motif-containing protein 5 TRIM5 TRIM5alpha RING-type E3 ubiquitin transferase TRIM5 protein protein macmu Macaca mulatta (Rhesus macaque) Rhesus macaque MASGILLNVKEEVTCPICLELLTEPLSLHCGHSFCQACITANHKKSMLYKEGERSCPVCRISYQPENIQPNRHVANIVEKLREVKLSPEEGQKVDHCARHGEKLLLFCQEDSKVICWLCERSQEHRGHHTFLMEEVAQEYHVKLQTALEMLRQKQQEAEKLEADIREEKASWKIQIDYDKTNVSADFEQLREILDWEESNELQNLEKEEEDILKSLTKSETEMVQQTQYMRELISELEHRLQGSMMDLLQGVDGIIKRIENMTLKKPKTFHKNQRRVFRAPDLKGMLDMFRELTDARRYWVDVTLAPNNISHAVIAEDKRQVSSRNPQIMYQAPGTLFTFPSLTNFNYCTGVLGSQSITSGKHYWEVDVSKKSAWILGVCAGFQSDAMYNIEQNENYQPKYGYWVIGLQEGVKYSVFQDGSSHTPFAPFIVPLSVIICPDRVGVFVDYEACTVSFFNITNHGFLIYKFSQCSFSKPVFPYLNPRKCTVPMTLCSPSS 950166879B2E1A6F trim5 2 5 unspecified role unspecified role neutral component neutral component c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spry motif required to bind necessary binding region required to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-923935]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 498-498, sequence length 333 [EBI-923935]298-498 direct interaction direct interaction Higher oligomers are observed. Deletions of 1-93 and 1-132 showed the presence of dimers, trimers and higher oligomers. These were more evident under reduced conditions induced by the use of beta-Mercaptoethanol. The TRIM5-alpha isoform was used. trim5-1 2 6 unspecified role unspecified role neutral component neutral component c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range direct interaction direct interaction Higher oligomers were observed. The TRIM5-alpha isoform was used. trim5-2 2 6 unspecified role unspecified role neutral component neutral component c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range direct interaction direct interaction The TRIM5-gamma isoform was used. trim5-3 1 6 unspecified role unspecified role neutral component neutral component c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range direct interaction direct interaction The TRIM5-alpha isoform was used. Mainly trimers and higher oligomers were observed in the presence of glutaraldehyde while dimers and trimers were observed in the presence of ethylene glycolbis(succinimidylsuccinate). trim5-4 2 3 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Some dimers and higher oligomers were also observed. trim5-5 2 4 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Some trimers were observed however the majority were higher oligomer fraction.