IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A unique sequence motif in the 54-kDa subunit of the chloroplast signal recognition particle mediates binding to the 43-kDa subunit. A unique sequence motif in the 54-kDa subunit of the chloroplast signal recognition particle mediates binding to the 43-kDa subunit. J. Biol. Chem. (0021-9258) 2005 Funke S., Knechten T., Ollesch J., Schunemann D. Directed yeast two hybrid assay used in this study Table III Table IV Danja.Schuenemann@ruhr-uni-bochum.de yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Funke S., Knechten T., Ollesch J., Schunemann D. Directed yeast two hybrid assay used in this study Table III Table IV Danja.Schuenemann@ruhr-uni-bochum.de Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Biol. Chem. (0021-9258) 2005 A unique sequence motif in the 54-kDa subunit of the chloroplast signal recognition particle mediates binding to the 43-kDa subunit. A unique sequence motif in the 54-kDa subunit of the chloroplast signal recognition particle mediates binding to the 43-kDa subunit. J. Biol. Chem. (0021-9258) 2005 Funke S., Knechten T., Ollesch J., Schunemann D. Directed yeast two hybrid assay used in this study Table III Table IV Danja.Schuenemann@ruhr-uni-bochum.de in vitro In vitro pull down pull down predetermined predetermined participant predetermined Funke S., Knechten T., Ollesch J., Schunemann D. Directed yeast two hybrid assay used in this study Table III Table IV Danja.Schuenemann@ruhr-uni-bochum.de Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Biol. Chem. (0021-9258) 2005 sr54c_arath Signal recognition particle 54 kDa protein, chloroplastic FFC At5g03940 F8F6_150 FFC CPSRP54 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEALQFSSVNRVPCTLSCTGNRRIKAAFSSAFTGGTINSASLSSSRNLSTREIWSWVKSKTVVGHGRYRRSQVRAEMFGQLTGGLEAAWSKLKGEEVLTKDNIAEPMRDIRRALLEADVSLPVVRRFVQSVSDQAVGMGVIRGVKPDQQLVKIVHDELVKLMGGEVSELQFAKSGPTVILLAGLQGVGKTTVCAKLACYLKKQGKSCMLIAGDVYRPAAIDQLVILGEQVGVPVYTAGTDVKPADIAKQGLKEAKKNNVDVVIMDTAGRLQIDKGMMDELKDVKKFLNPTEVLLVVDAMTGQEAAALVTTFNVEIGITGAILTKLDGDSRGGAALSVKEVSGKPIKLVGRGERMEDLEPFYPDRMAGRILGMGDVLSFVEKATEVMRQEDAEDLQKKIMSAKFDFNDFLKQTRAVAKMGSMTRVLGMIPGMGKVSPAQIREAEKNLLVMEAMIEVMTPEERERPELLAESPERRKRIAKDSGKTEQQVSALVAQIFQMRVKMKNLMGVMEGGSIPALSGLEDALKAEQKAPPGTARRKRKADSRKKFVESASSKPGPRGFGSGN DIP protein P37107 original sequence version: 105 423F7285FB9063E4 sr43c_arath Signal recognition particle 43 kDa protein, chloroplastic CAO T30B22.25 At2g47450 Chromo protein SRP43 CPSRP43 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQKVFLAMDTCALVIHQSLSRIKLSPPKSSSSSSSAFSPESLPIRRIELCFRGAICAAVQRNYEETTSSVEEAEEDDESSSSYGEVNKIIGSRTAGEGAMEYLIEWKDGHSPSWVPSSYIAADVVSEYETPWWTAARKADEQALSQLLEDRDVDAVDENGRTALLFVAGLGSDKCVRLLAEAGADLDHRDMRGGLTALHMAAGYVRPEVVEALVELGADIEVEDERGLTALELAREILKTTPKGNPMQFGRRIGLEKVINVLEGQVFEYAEVDEIVEKRGKGKDVEYLVRWKDGGDCEWVKGVHVAEDVAKDYEDGLEYAVAESVIGKRVGDDGKTIEYLVKWTDMSDATWEPQDNVDSTLVLLYQQQQPMNE DIP protein O22265 original sequence version: 82 DIP protein O22265 original sequence version: 98 F75ED9C7046A1441 ffc-scj4_30 1 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P37107:p.Arg537Gly mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R G Wed May 02 15:54:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg537gly' Tue Jun 30 19:12:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg537Gly' P37107:p.Arg536Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R G Wed May 02 16:46:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg536gly' Tue Jun 30 18:31:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg536Gly' P37107:p.[Arg536Gly;Lys538_Lys540delinsMetGlyMet] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R G certain certain sequence position certain certain certain sequence position certain KRK MGM Fri Jul 22 10:57:18 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg536gly,lys538met,arg539gly,lys540met' Wed May 02 18:53:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg536gly,lys_arg_lys538-540met_gly_met' Tue Jun 30 17:15:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg536Gly;Lys538_Lys540delinsMetGlyMet]' physical association physical association ffc-scj4_30-1 2 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P37107:p.Arg537Gly mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R G Wed May 02 15:41:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg537gly' Tue Jun 30 20:09:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg537Gly' P37107:p.[Arg537Gly;Lys538Met] arg537gly&lys538met mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R G certain certain sequence position certain certain certain sequence position certain K M Wed May 02 18:55:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg537gly,lys538met' Tue Jun 30 19:08:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg537Gly;Lys538Met]' P37107:p.Arg537Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R G Wed May 02 19:17:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg537gly' Tue Jun 30 17:54:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg537Gly' P37107:p.Arg539Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R G Wed May 02 14:52:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg539gly' Tue Jun 30 16:41:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg539Gly' direct interaction direct interaction A synthetic peptide corresponding to residues 534 to 540 amio acids of FFC at 7.5 mico-molar completely eliminates binding between the proteins.