UniProt UniProt http://www.uniprot.org KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans. KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans. Genes Dev. (0890-9369) 2003 Desai A., Rybina S., Muller-Reichert T., Shevchenko A., Shevchenko A., Hyman A., Oegema K. koegema@ucsd.edu hyman@mpi-cbg.de caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. fluorescence imaging fluorescence microscopy fluorescence imaging polyclonal immunost polyclonal antibody immunostaining polyclonal immunost Desai A., Rybina S., Muller-Reichert T., Shevchenko A., Shevchenko A., Hyman A., Oegema K. koegema@ucsd.edu koegema@ucsd.edu Accepted 2006-SEP-26 by JYOTI. Genes Dev. (0890-9369) 2003 KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans. KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans. Genes Dev. (0890-9369) 2003 Desai A., Rybina S., Muller-Reichert T., Shevchenko A., Shevchenko A., Hyman A., Oegema K. koegema@ucsd.edu hyman@mpi-cbg.de caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Desai A., Rybina S., Muller-Reichert T., Shevchenko A., Shevchenko A., Hyman A., Oegema K. koegema@ucsd.edu koegema@ucsd.edu Accepted 2006-SEP-26 by JYOTI. Genes Dev. (0890-9369) 2003 KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans. KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans. Genes Dev. (0890-9369) 2003 Desai A., Rybina S., Muller-Reichert T., Shevchenko A., Shevchenko A., Hyman A., Oegema K. koegema@ucsd.edu hyman@mpi-cbg.de caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Desai A., Rybina S., Muller-Reichert T., Shevchenko A., Shevchenko A., Hyman A., Oegema K. koegema@ucsd.edu koegema@ucsd.edu Accepted 2006-OCT-9 by JYOTI. Genes Dev. (0890-9369) 2003 bub1_caeel Mitotic checkpoint serine/threonine-protein kinase bub-1 bub-1 Budding uninhibited by benzimidazole 1 R06C7.8 protein protein caeel Caenorhabditis elegans MSHIRVAFAPLDTNPSTCGLETFATQIETLSQMMTAEEMKAELYDCVRRILREQKFDQIRENEAMLRLYKVMGRSSTNLKGRGIYEQLFKKDHFTGSLKFYLQWAEECGKDQMLEEFKDVLKLARDRLSERIEMTAIESGFRDLVDEYFNGESGDMFTRPDETMDLFRFNAGSKKKRRSSVCFLQHNVPINNSGKAAFGPKTKTDLRQACIDRPNYHGISIEEFRFAKWKDTFGEDVDDDYRKRKDSGVVFVKHQVIDTDRQAREEVENRFNANLNPRRRHLSPVSEKTVDDEEEKRSRIYSPLVATKDAHRPALRSKIENPPATVTLSSDTKSASEKDVSDSDDADDDERLKIMTAGRKDGNPPDRSTSISSNYSTASARTSKSGAGLDLMAENKCLEAHAMFSDTVHLASEKTMVLGDDSVFVPERSLATTQIVTDFSVLCDPDPTMTITQERPKKVSNGLNVVYDEAAEPEESQKVEESEVQPEIVLVSPVTQTSPATMFNDIYDDEIEFGFFKPSRGNFVTSTPAQGVHLVNIDEYFGNKEEESTHEQEAPVFVAPTSSTFSKLTRRKSLAANQAVQPSVTESSKPERSDPKDSSIDCLTANLGRRLSIGADEIPNLTENNESEITGCKIRRRSEIIKQGDINPWDETLRKKLMCLVRPPQNMHEFQERAPKIQALRDCEVSGEKLHIQTLIGQGGYAKVYRAVTDDQRTVAVKYEVPSCSWEVYICDQMRNRLKDRGLELMAKCCIMEVMDAYVYSTASLLVNQYHEYGTLLEYANNMKDPNWHITCFLITQMARVVKEVHASKIIHGDIKPDNFMITRKIDDKWGKDALMSNDSFVIKIIDWGRAIDMMPLKNQRFKGRAGTEAFDCPEMVDGRSWTYQADYFGFAATMAVVVAGKYAQLTGASVGDYSLNVDIKRRNILRDACYDVINRFLNIPSCDSLPDWNILIKSFSEIWNEKFEASGWRQAVSKFNEACDLAANQK 48E7915551AE2194 g5eda3_caeel hcp-4 CELE_T03F1.9 T03F1.9 protein protein caeel Caenorhabditis elegans MNRKPRTRSTIVPGRKMITEIVALREAGLDDTRPSYLEEPTVIVDESMMNDSANLEEREWRKQGLSEKQMFAILEKRKQTQLNNARKRLEKEMYGAKTFKELIACREYSECESDSENTVNQNVRSASVFAIPALPKHISEKSMMGSPIANSRGSGKAGLSCSTPKSSSDTSMRSLRSLDISHVVNTDRLDAERVTVHTKSVVIPTILEERESTLQKTLTIEKNHSSQLQKTFTVAEDAPEELQKTVTIEKNQSSEMQRTFTVAKDASKEQSQLQRTVTIEKNQSSQLQGNFTVAKDAPEELQKTVTIEKNQSSEMQGTVTVAKDAEHSSLQKTFTKPTEKDESSLQRTFNVANRDENNDSTLQKTFIIEERDAYEQGTTSVGVKPPVLAQNTMEGGEKRRTSKVTSDERERRNASISNPLHNSMLEKEEIQSPGANFFKHPRKKIRPVVQTPPRIKATSRLSTESNKERTIEMESVAEERTMEADSIGSSYISPTFNASRVSPVPEVPEKVEPMHVSKAATPKSIRHTDNSIRNIGPTNNVECARAALLSTPTRMDIVDSVNRVTPARRYEQPTFASLVKRMNMKDANRLLEETSRKNTPAKTTATTSSAAVRMVLEDDEEDQATEVIEKRSENGGVIVDGEDEAADSSNRSLNIELNALTVNEEPAHDISAIDFPEDDTNEMRSSSDEEEMEARPDPRKKSSRRLGLLSDSIALGLASSSRRRPNDTFVDETWYPEPNSKGNRRPRTHRGMKLKEHQLMKPEDAPDGVRRSTRVRVKPVRSWLGEQPVYVNSPISGCKRLTGVTAVVIKDPRLCYYRTADVRTATERELKDKANKRALAQEKKQQRQNARSGRRHDSDDDEEDDM 56FA16B45C049BC2 ndc80_caeel Kinetochore protein ndc-80 ndc-80 W01B6.9 Hec1 homolog protein protein caeel Caenorhabditis elegans MFGDRRKTGGLNLNGRASIAITPTKRFTDYTGSTSVRKTDARPSLSQPRVSLFNTKNSSVAPRDVKSLVSLNGSKIYNFLVEYESSDAPSEQLIMKPRGKNDFIACFELIYQHLSKDYEFPRHERIEEEVSQIFKGLGYPYPLKNSYYQPMGSSHGYPHLLDALSWLIDIIRINSAVSEDTQNILFGDFMEQGKAQEKTLNYAWMTSTFRDYTNDRKAAENPSSSYWDDTKHRLRKYFEQSNEFEDMTKTAASALEMLNYECDEIEADKGNEASLKEEISRIRDDIRKAKDYLEQNLHVKQHMEKELAMVKSEQEEKISENEKVQKMVDDLKNKIELQKQIHGLTGKEVRQMNLDNNKDKEVVLEIQSELDRLSKETWKLKDEDFFKEQKSKFIHLAEQIMKILSGLNIQMNLEPLRAPTNERDLKDYWETLNKIWVPEISRQLHQRKLELETEQSRFSNKAVTAEERIQIQSETLCEAKKNEAREERIRRNERDSWKDARKHIEQRYEQLLNEKEVLLKQMKLDGSLEKEIEDETARMSATGEEHIQKRSQLEAGIRQILDLMVVEIAEIENKKIGFHVQCAGIEKAVL 77548C2EE701E8CD nuf2_caeel Kinetochore protein Nuf2 homolog R12B2.4 Kinetochore protein him-10 Protein high incidence of males 10 him-10 protein protein caeel Caenorhabditis elegans MSNVVLIVYDPRMISKYLGQKLHMGLVADDIIKPTAEIAQQIFANFVRLVLNVSESSLTTLPLSANCDYDPELHKKSIPIIILFQCMKAFIKDNSGNKLDLTMCDLVTPAKHEHRFRKLTSFLVDFLKLHELATPAFNEISEEFSDRKFEMEKIREELLEAEKKKNDLLAKQSIRKRHEHELINEQSNAKAELKNVVNEYTETRQINEELDKQKEEAILHIQALEKEMLTGKKTIEHLNEEVLTSPEQLKQEMEERKRHIEELRDCLESSKKGLQAKLEAREICINSEKNVPVIIEKIHQWTEVREVIIDLIDVESENLRKLKEMEEQLDFMMKEMETAQKRLVEQSETHEQLRIEHTQKSEERQRRIEEITEQIANLKTSQPDVSQEIAKKKQELLALKNAHSETISQITNSCQDAVAKFAKLNAMFKETQKVAFEKNTAAAREMERLKSSLTGRLLSDYTFGSSTIDAGENTENCDPQPNDSSFSVFK 7247744354F3E211 knl1_caeel Kinetochore null protein 1 C02F5.1 knl-1 protein protein caeel Caenorhabditis elegans MSMEPRKKRNSILKVRQAVETIEETVMNSGPSSTTTNRRVSFHNVKHVKQYDRDHGKILDATPVKEKITDTIGSDGILTPRGGNMDISESPACTSSFQVFGGGNLDKTMDMSLETTINENNETARLFETTRDPTLLYEKIVETTTKVTERIVSMPLDDTLAMFNTTNQEDKDMSVDRSVLFTIPKVPKHNATMNRTIPMDLDESKAAGGQCDETMNVFNFTNLEAAEMDTSKLDENNTMNAIRIPINSNVMPVDMDITEHHTLIEEKKNDTFGPSQLMDISAPQVQVNDTLAIFNSPRDICNKGLGVPQNLINIASNVVPVDMDITDQAVLNAEKKNDQFETSQLMDISIPKVLVNDTMAMFNSPKHVSKSSMDLEKTIEAADKSTKYPSIADEVEDLDMDMDITEQQPCEAGNQQNDGLQLQKEDLMDISVIRDSPAVNDTMAVFQSPARVKIGANNSIIDSQKSIVFGDEMSIDETQNDGTLTLPKSNVEVTTTNDVYTSLERQEENASENVSMINESSVHSEIDKKSFMLIEEERAFMHSSMIDVAQKLEDDGSSKTPVILASQSASLATKEPSALHNSSATLNNSMELDNNTLLKTMQITTCEDISMVHESIAVELNSNKEQEQFGDETLQKNDTSNTGANFTFQGHNETSQIMNNVDSEAVNTSKISTYSAFNLSINQSISKRRRSLLNSARESPRRVALENSIMSMNGQTMEALTEYRQNKTMQTSQDSMPSMSLNDSGRDILAMNTSVRSPHLNSSKTAAPGTPSLMSQNVQLPPPSPQFEMPDFDPAVVNVVYLTSEDPSTEQHPEALKFQRIVENEKMKVQHEIDSLNSTNQLSAEKIDMLKTKELLKFSHDEREAIMIARKDAEIKFLELRLKFALEKKIESDQEIAELEQGNSKMAEQLRGLDKMAVVQKELEKLRSLPPSREESGKIRKEWMEMKQWEFDQKMKALRNVRSNMIALRSEKNALEMKVAEEHEKFAQRNDLKKSRMLVFSKAVKKIVNF 15E19DD975824D94 knl-1_ndc-80_1 3 8 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey association association Fig. 4A knl-1_ndc-80_2 2 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait association association Fig. 4C hcp-4_knl-1_1 2 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey association association Fig. 4D ndc-80_him-10_1 1 7 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 5A hcp-4_knl-1_3 1 5 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 7B hcp-4_knl-1_2 1 5 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 2C