UniProt UniProt http://www.uniprot.org Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor. Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor. J. Biol. Chem. (0021-9258) 2002 imex curation Nemergut ME., Lindsay ME., Brownawell AM., Macara IG. note for Ran mutant: SP changed seq/position (init_met) mel3f@virginia.edu 13-SEP-2006: Contacted by JYOTI Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Nemergut ME., Lindsay ME., Brownawell AM., Macara IG. note for Ran mutant: SP changed seq/position (init_met) mel3f@virginia.edu Accepted 2006-AUG-24 by ORCHARD. 13-SEP-2006: Contacted by JYOTI J. Biol. Chem. (0021-9258) 2002 Only protein-protein interactions imex curation Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor. Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor. J. Biol. Chem. (0021-9258) 2002 imex curation Nemergut ME., Lindsay ME., Brownawell AM., Macara IG. note for Ran mutant: SP changed seq/position (init_met) mel3f@virginia.edu 13-SEP-2006: Contacted by JYOTI Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Nemergut ME., Lindsay ME., Brownawell AM., Macara IG. note for Ran mutant: SP changed seq/position (init_met) mel3f@virginia.edu Accepted 2006-AUG-24 by ORCHARD. 13-SEP-2006: Contacted by JYOTI J. Biol. Chem. (0021-9258) 2002 Only protein-protein interactions imex curation xpo1_human Exportin-1 CRM1 XPO1 Chromosome region maintenance 1 protein homolog protein protein human Homo sapiens Human MPAIMTMLADHAARQLLDFSQKLDINLLDNVVNCLYHGEGAQQRMAQEVLTHLKEHPDAWTRVDTILEFSQNMNTKYYGLQILENVIKTRWKILPRNQCEGIKKYVVGLIIKTSSDPTCVEKEKVYIGKLNMILVQILKQEWPKHWPTFISDIVGASRTSESLCQNNMVILKLLSEEVFDFSSGQITQVKSKHLKDSMCNEFSQIFQLCQFVMENSQNAPLVHATLETLLRFLNWIPLGYIFETKLISTLIYKFLNVPMFRNVSLKCLTEIAGVSVSQYEEQFVTLFTLTMMQLKQMLPLNTNIRLAYSNGKDDEQNFIQNLSLFLCTFLKEHDQLIEKRLNLRETLMEALHYMLLVSEVEETEIFKICLEYWNHLAAELYRESPFSTSASPLLSGSQHFDVPPRRQLYLPMLFKVRLLMVSRMAKPEEVLVVENDQGEVVREFMKDTDSINLYKNMRETLVYLTHLDYVDTERIMTEKLHNQVNGTEWSWKNLNTLCWAIGSISGAMHEEDEKRFLVTVIKDLLGLCEQKRGKDNKAIIASNIMYIVGQYPRFLRAHWKFLKTVVNKLFEFMHETHDGVQDMACDTFIKIAQKCRRHFVQVQVGEVMPFIDEILNNINTIICDLQPQQVHTFYEAVGYMIGAQTDQTVQEHLIEKYMLLPNQVWDSIIQQATKNVDILKDPETVKQLGSILKTNVRACKAVGHPFVIQLGRIYLDMLNVYKCLSENISAAIQANGEMVTKQPLIRSMRTVKRETLKLISGWVSRSNDPQMVAENFVPPLLDAVLIDYQRNVPAAREPEVLSTMAIIVNKLGGHITAEIPQIFDAVFECTLNMINKDFEEYPEHRTNFFLLLQAVNSHCFPAFLAIPPTQFKLVLDSIIWAFKHTMRNVADTGLQILFTLLQNVAQEEAAAQSFYQTYFCDILQHIFSVVTDTSHTAGLTMHASILAYMFNLVEEGKISTSLNPGNPVNNQIFLQEYVANLLKSAFPHLQDAQVKLFVTGLFSLNQDIPAFKEHLRDFLVQIKEFAGEDTSDLFLEEREIALRQADEEKHKRQMSVPGIFNPHEIPEEMCD DIP protein O14980 original sequence version: 98 DIP protein O14980 original sequence version: 120 DIP protein O14980 original sequence version: 97 DIP protein O14980 original sequence version: 96 DIP protein O14980 original sequence version: 116 DIP protein O14980 original sequence version: 129 DIP protein O14980 original sequence version: 130 FDB00C065DA2FB1D ran_human GTP-binding nuclear protein Ran GTPase Ran Ras-related nuclear protein Ras-like protein TC4 Androgen receptor-associated protein 24 RAN ARA24 OK/SW-cl.81 protein protein human Homo sapiens Human MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL DIP protein P62826 original sequence version: 138 DIP protein P62826 original sequence version: 67 DIP protein P62826 original sequence version: 93 DIP protein P62826 original sequence version: 125 DIP protein P62826 original sequence version: 96 D5C9B7275C34BCE0 rcc1_human Regulator of chromosome condensation Chromosome condensation protein 1 Cell cycle regulatory protein RCC1 CHC1 protein protein human Homo sapiens Human MSPKRIAKRRSPPADAIPKSKKVKVSHRSHSTEPGLVLTLGQGDVGQLGLGENVMERKKPALVSIPEDVVQAEAGGMHTVCLSKSGQVYSFGCNDEGALGRDTSVEGSEMVPGKVELQEKVVQVSAGDSHTAALTDDGRVFLWGSFRDNNGVIGLLEPMKKSMVPVQVQLDVPVVKVASGNDHLVMLTADGDLYTLGCGEQGQLGRVPELFANRGGRQGLERLLVPKCVMLKSRGSRGHVRFQDAFCGAYFTFAISHEGHVYGFGLSNYHQLGTPGTESCFIPQNLTSFKNSTKSWVGFSGGQHHTVCMDSEGKAYSLGRAEYGRLGLGEGAEEKSIPTLISRLPAVSSVACGASVGYAVTKDGRVFAWGMGTNYQLGTGQDEDAWSPVEMMGKQLENRVVLSVSSGGQHTVLLVKDKEQS DIP protein P18754 original sequence version: 117 F6D225AF81928305 ranb3_human Ran-binding protein 3 RANBP3 protein protein human Homo sapiens Human MADLANEEKPAIAPPVFVFQKDKGQKSPAEQKNLSDSGEEPRGEAEAPHHGTGHPESAGEHALEPPAPAGASASTPPPPAPEAQLPPFPRELAGRSAGGSSPEGGEDSDREDGNYCPPVKRERTSSLTQFPPSQSEERSSGFRLKPPTLIHGQAPSAGLPSQKPKEQQRSVLRPAVLQAPQPKALSQTVPSSGTNGVSLPADCTGAVPAASPDTAAWRSPSEAADEVCALEEKEPQKNESSNASEEEACEKKDPATQQAFVFGQNLRDRVKLINESVDEADMENAGHPSADTPTATNYFLQYISSSLENSTNSADASSNKFVFGQNMSERVLSPPKLNEVSSDANRENAAAESGSESSSQEATPEKESLAESAAAYTKATARKCLLEKVEVITGEEAESNVLQMQCKLFVFDKTSQSWVERGRGLLRLNDMASTDDGTLQSRLVMRTQGSLRLILNTKLWAQMQIDKASEKSIRITAMDTEDQGVKVFLISASSKDTGQLYAALHHRILALRSRVEQEQEAKMPAPEPGAAPSNEEDDSDDDDVLAPSGATAAGAGDEGDGQTTGST 203B5A900512743C ranbp3-ran-1 1 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey P62826:p.Gln69Leu constitutive active (GTP-bound) mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 19:15:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln69leu' Tue Jun 30 19:08:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln69Leu' in vitro In vitro physical association physical association 1B and C Interaction of RANBP3 with Ran-GDP is found in presence of RCC1 (UniProt Ac: P18754). ranbp3-rcc1-1 1 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region Ran_BP1 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey in vitro In vitro physical association physical association 1C and D Interaction is enhanced by Ran-GTP (UniProt Ac: P62826). We now report that RanBP3 associates with the Ran-specific guanine nucleotide exchange factor, regulator of chromosome condensation 1 (RCC1). rcc1-xpo1-1 1 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role prey prey in vitro In vitro physical association physical association 3A and B Interaction has only been observed in presence of RANBP3 (UniProt Ac: Q9H6Z4) and RAN (UniProt Ac: P62826). RAN-GDP appears to be more efficient in recruiting XPO1 to RCC1 than RAN-GTP. rcc1-ranbp3-1 2 6 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 5 unspecified role unspecified role bait bait P18754:p.Asp182Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain D A Wed May 02 16:01:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp182ala' Tue Jun 30 19:52:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp182Ala' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association 1A We now report that RanBP3 associates with the Ran-specific guanine nucleotide exchange factor, regulator of chromosome condensation 1 (RCC1).