UniProt UniProt http://www.uniprot.org Two WD repeat-containing TATA-binding protein-associated factors in fission yeast that suppress defects in the anaphase-promoting complex. Two WD repeat-containing TATA-binding protein-associated factors in fission yeast that suppress defects in the anaphase-promoting complex. J. Biol. Chem. (0021-9258) 2001 Mitsuzawa H., Seino H., Yamao F., Ishihama A. hmitsuza@lab.nig.ac.jp 08-DEC-2006: Contacted by JYOTI. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mitsuzawa H., Seino H., Yamao F., Ishihama A. hmitsuza@lab.nig.ac.jp Accepted 2006-DEC-13 by DAVET. 08-DEC-2006: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2001 Two WD repeat-containing TATA-binding protein-associated factors in fission yeast that suppress defects in the anaphase-promoting complex. Two WD repeat-containing TATA-binding protein-associated factors in fission yeast that suppress defects in the anaphase-promoting complex. J. Biol. Chem. (0021-9258) 2001 Mitsuzawa H., Seino H., Yamao F., Ishihama A. hmitsuza@lab.nig.ac.jp 08-DEC-2006: Contacted by JYOTI. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Mitsuzawa H., Seino H., Yamao F., Ishihama A. hmitsuza@lab.nig.ac.jp Accepted 2006-DEC-13 by DAVET. 08-DEC-2006: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2001 tbp_schpo TATA-box-binding protein TATA-box factor TATA-binding factor TATA sequence-binding protein Transcription initiation factor TFIID TBP subunit tbp1 SPAC29E6.08 SPAC30.12 tbp tdf1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDFALPTTASQASAFMNNSSLTFPVLPNANNEATNETADSGDAEVSKNEGVSGIVPTLQNIVATVNLDCRLDLKTIALHARNAEYNPKRFAAVIMRIREPKSTALIFASGKMVVLGGKSEDDSKLASRKYARIIQKLGFNAKFTDFKIQNIVGSCDVKFPIRLEGLAYSHGTFSSYEPELFPGLIYRMVKPKVVLLIFVSGKIVLTGAKVREEIYQAFEAIYPVLSEFRKH EBFB6D08BA2B6283 taf7_schpo Transcription initiation factor TFIID subunit 7 taf7 ptr6 SPAC13F5.02c Transcription initiation factor TFIID 55 kDa subunit protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MVKLKIRAVQPPPNDSRSSTPATGPPPPIPKIKIKTREPKGPRLTKIRLKRVREPGLGYDSEASDREEDTYIEEQIILRLPPGEDCEYVRKAIENREVGRGADIWVKFKDQRRAVVHVNGHLYAAKLVDLPCIIESNKSFDKKVIFKAADICQMLIATERIEHENSVLNTQLKQADYIYPHGLTTPMHWVRQKRFRKRVSNRTIEAVENEVDRLLAMDERAESTSNELIDQAQLARDSSIALSEDTSFDGMAGLRGTSIDRDDQSVQTDMFDGMDEDDLAGQIEQGMLELSQDTRESTAEPRAAGEESASEEEEEEEEEEEEENEADDETRENKRQNRLVREFISELESSIQKRRKDADEATNPILRNRFLADVNRMVTELELKRTQLVDNPE 58234FBA16998B5B t111_schpo Putative transcription initiation factor TFIID 111 kDa subunit SPAC2G11.14 TAFII-111 TBP-associated factor 111 kDa protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSFDGLIVENENTKSGYNDGNDLTDLFKQNGTDMSVINSLLGDTNNPGMNESPKILDSSFENSNPQDGPNYEDFDFMGSIHKEFGNNINEMDDMEDVSDDNLPEEEQAVNYTGDKDDEDFGKLLAKEMGEEAAGQVLSGVGFSIPSGLVPPSEPSKTVSSTTEELQNEAQIRESIVKTFFPTFERGVLLNFSELFKPKPVKLAPPKKKTPKVCVPGRLTLEVDTDYAIIFNSKKSLPLKRNVVSPISTHTKKRRRTANTSQRNDGLDLNTVFTTNDWEKNIIYDESDVNKTNQSSFFIDKSLVDIDFAFDENIFDGDTGTSKVVLNLNDPKLLLQPQLPKKEDSQRSFADTHQRNSLAWKFNISNDPAYEMLKQNHQSKVRNTLSQLAIEHAAFAEKLTFPYYKTRLSKRAVRSYHRPTMSFKPNAAIVFSPLIVRKRSKDKHKSERELIPTTKEITMGDTTHAILVEFSEEHPAVLSNAGMASRIVNYYRKKNEQDESRPKLEVGESHVLDVQDRSPFWNFGSVEPGEITPTLYNKMIRAPLFKHEVPPTDFILIRNSSSYGSKYYLKNINHMFVSGQTFPVTDVPGPHSRKVTTASKNRLKMLVFRLIRRSPNGGLFIRQLSKHFSDQNEMQIRQRLKEFMEYKKKGDGPGYWKLKSNEVVPDEAGTRSMVSPETVCLLESMQVGVRQLEDAGYGKTMDEINDDEDEEQPAEQLLAPWITTRNFINATQGKAMLTLFGEGDPTGIGEGYSFIRTSMKGGFKPAGETADDKPEPQTKNAHAYNVAKQQRAYEEEINRIWNAQKRGLSINNLEELAKKYGINSIHDDYVESNEETTREETPSSDKVLRIVRLYRDKNGNLERKQETIHDPIVIHAYLKKRREIDEQSTALDAVVPTGDEAIDRRNRRRLEQELAKSQKNWERRRARHAAKEGINLNGEGRKPTTRKCSNCGQVGHMKTNKICPLFGRPEGGLATMLDKN 37873DCC23884701 37873DCC23884701 gcn5_schpo Histone acetyltransferase gcn5 gcn5 SPAC1952.05 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSNSLNDQTRPPDSSVVDSTSSNFPNASNDSVHDKPVKQDEQSNPNVREAIANSDGQESLAVKENKDTESSSSHFVPNGVSNSKKRKLVATDLDVDFDISSVRVTEKPSVLEEKSGVIQFRVVSNDDTADSMIMLTGLKNIFMKQLPKMPKEYITRLIYDRNHLSMTIVKDNLHVVGGITYRPFEQRGFAEIVFCAIASNEQVRGYGSHLMNHLKDYVRGTTTIQHFLTYADNYAIGYFKKQGFTKEITLDKSIWVGYIKDYEGGTLMQCTMIPKIKYLEANLILAIQKAAVVSKINRITRSNVVYPGLDVFKDGPAHIEPSQVPGLMEVGWCKEMEELSKKPRPKPFFAVLEMLFTEMQNHPSSWPFMQPVSKEDVPDYYEVIEHPMDLSTMEFRLRNNQYESVEEFIRDAKYIFDNCRSYNDSNTTYYKNADRLEKFFQKKLRETEYSHLAD FF99039B00E8ECB3 taf73_schpo Transcription initiation factor TFIID subunit taf73 Transcription initiation factor TFIID 73 kDa subunit taf73 SPBC15D4.14 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MAQSSYSSGNSTSDLNKIVLDYLFKKGYSRTEAMLRLEISSVGAIPEEQQDWHIGASETYVQVYILLRDWIDGTLDLYKPKLQKVLYPIFVHSYLDLLQKNDPEMAIYFFESFRIEHEVLHGYDIRALAQLKIASDVEEIEIAQLYRKNKYRINFTRSTFDLLVQFLFENEVNGSGIIIRLLNQYIDIKITMPQVEGKEDPNVQAVYEMDEAKNEQTIISEQEGIPGHSEQLLDYNKQSIQLGLRPLSEEVVTTLKNLLEVKDKDVEGRNEALNKILHPAKNLVELLTEKENLINESTLESPSDPPLPPTKMKDIDYFVSLLESEQNSLILGKNATDPSVFMYTMHNTLSAVNCAAFSDDASMFALGCADSSIHLYSSTNNGPQPLVGSQNEPLQKSSLIGHTRPVFGVSISPQKEFILSCSEDGFTRLWSKDTKSTIVKYAGHNAPIWDVQFSPFGYYFATASHDQTARLWDVEHAAPLRVFVGHQNDVDCVSFHPNAAYLATGSSDHTTRMWDVRTGGTVRVFNAHHSPVSALCMSADGLSLASADESGIIKVWDLRSSNQHVSFVKHSSIVYSLSFSYDNKILVSGGADTDVNFWDLTYRNNLSDGEQSPLLFNFKTKNTIIQNLSFTRRNYCLALSSS 6891234235FF6DBE taf5_schpo Transcription initiation factor TFIID subunit 5 taf5 taf72 SPCC5E4.03c Transcription initiation factor TFIID 72 kDa subunit protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSATNGPQPQDLNRIVLDYLAKKGYSRTEAMLRLEASGSGVSVEEKLKSIEETPDAYTHTYTILRDWVDSSLELYKAELHRILFPIFVHSYLNLLSQDHYEAAKQFYELFKDDHTDLHDFDVKNLKSLSLPSHVAEDRTAQQYRQNKYQLHFSRITFDLLLHFLFENVSNGGSIIIKLINQHIDIHIVPGRPTVLENAKVINEQEGITGQSFERGDAQLQPVKLQQMPMDKEMEKIVEMDLEEEDMMHQNDPNNQSPKLLKEFRKLHEPNAEDAPSRDYIPLPPHKGVDILSEVEAVKDWSKRLHLGPRASLPSVCMYTFHHTNNNMNCAEFSPDSTMIACGFQESYIRLWSIKADKKSLPKSTSVEDSDGSVRLLSHSGPVYGTTFSPDNKYLLSCSEDASARLWSVDTKTALVAYKGHTGPVWDVAFGPFGHYFATASHDQTAQLWSCDHIYPLRVFAGHLSDVDCVTFHPNSAYVLTGSSDKTCRLWDVHRGHSVRVFNGHTQPVTAVAIAPDGHTMASADSEGLIHLWDIGTGRRIKTMRGHRGNIYSLSFSRESTVLVSGGSDCTVRAWDVFKTNYNNPVSSSLTGSVVTPFSAKTSTFNEVNWSTSPDQMVALYTKQTPIFNVSFTRRNLCLAISVS 859434527430F3B1 taf73-comp 2 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Fig. 6A Association of TAF72 and TAF73 with TBP. taf72-comp 2 3 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey association association Fig. 6A Association of TAF72 and TAF73 with TBP. taf73-taf72 2 8 unspecified role unspecified role prey prey n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 7 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 6C co-immunoprecipitation of TAF73 with TAF72. taf72-gcn5 2 8 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role prey prey n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 7 Association of TAF72 with Gcn5. taf72-comp-1 1 3 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey association association Fig. 6B immunoprecipitation of TAF72 or TAF73 with TBP. taf73-comp-1 1 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Fig. 6B immunoprecipitation of TAF72 or TAF73 with TBP.