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  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>IntAct</shortLabel>
        <fullName>European Bioinformatics Institute</fullName>
        <alias type="synonym" typeAc="MI:1041">IntAct</alias>
        <alias type="synonym" typeAc="MI:1041">intact</alias>
      </names>
      <bibref>
        <xref>
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        </xref>
      </bibref>
      <xref>
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      </xref>
      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/</attribute>
        <attribute name="search-url" nameAc="MI:0615">http://www.ebi.ac.uk/intact/query/${ac}</attribute>
        <attribute name="id-validation-regexp" nameAc="MI:0628">EBI-[0-9]+|IA:[0-9]+</attribute>
        <attribute name="definition">INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions.</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact</attribute>
        <attribute name="postaladdress">European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact/</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Twists in catalysis: alternating conformations of Escherichia coli thioredoxin reductase.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="10947986" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1991529" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-27304" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Twists in catalysis: alternating conformations of Escherichia coli thioredoxin reductase.</attribute>
            <attribute name="journal" nameAc="MI:0885">Science (0036-8075)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2000</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Lennon B.W., Williams C.H., Ludwig M.L.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="pubmed" dbAc="MI:0446" id="10947986" refType="primary-reference" refTypeAc="MI:0358"/>
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        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>x-ray diffraction</shortLabel>
            <fullName>x-ray crystallography</fullName>
            <alias type="go synonym" typeAc="MI:0303">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">x-ray diffraction</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0114" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1272" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Lennon B.W., Williams C.H., Ludwig M.L.</attribute>
          <attribute name="accepted"/>
          <attribute name="journal" nameAc="MI:0885">Science (0036-8075)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2000</attribute>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>trxb_ecoli</shortLabel>
          <fullName>Thioredoxin reductase</fullName>
          <alias type="gene name" typeAc="MI:0301">trxB</alias>
          <alias type="locus name" typeAc="MI:0305">b0888</alias>
          <alias type="locus name" typeAc="MI:0305">JW0871</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P0A9P4" version="SP_28" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="P09625" version="SP_28" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="ensemblbacteria" id="AAC73974" version="SP_134" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="BAA35613" version="SP_134" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1029826" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_415408.1"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0019430" version="SP_134"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0050660" version="SP_134"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR005982" version="SP_134"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR008255" version="SP_134"/>
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          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1TRB" version="SP_134"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="WP_000537418.1"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-6168N"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
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          </xref>
        </interactorType>
        <organism ncbiTaxId="83333">
          <names>
            <shortLabel>ecoli</shortLabel>
            <fullName>Escherichia coli (strain K12)</fullName>
          </names>
        </organism>
        <sequence>MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQLTTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNGEYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVEEALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGVTGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQTSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK</sequence>
        <attributeList>
          <attribute name="crc64">8E5AF86FB195CC82</attribute>
        </attributeList>
      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>thio_ecoli</shortLabel>
          <fullName>Thioredoxin 1</fullName>
          <alias type="gene name" typeAc="MI:0301">trxA</alias>
          <alias type="gene name synonym" typeAc="MI:0302">fipA</alias>
          <alias type="gene name synonym" typeAc="MI:0302">tsnC</alias>
          <alias type="locus name" typeAc="MI:0305">b3781</alias>
          <alias type="locus name" typeAc="MI:0305">JW5856</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P0AA25" version="SP_26" refType="identity" refTypeAc="MI:0356"/>
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          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="P76750" version="SP_26" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q2M889" version="SP_26" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q47674" version="SP_26" refType="secondary-ac" refTypeAc="MI:0360"/>
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          <secondaryRef db="ensemblbacteria" id="BAE77517" version="SP_153" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-368542" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="WP_001280776.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_418228.2"/>
          <secondaryRef db="ensemblbacteria" id="AAC76786" version="SP_153" refType="transcript"/>
          <secondaryRef db="ensemblbacteria" id="b3781" version="SP_153" refType="gene"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0015035" version="SP_153"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016671" version="SP_153"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0030337" version="SP_153"/>
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          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR017937" version="SP_153"/>
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          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1KEB" version="SP_153"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1M7T" version="SP_153"/>
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          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1SKR" version="SP_153"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1SKS" version="SP_153"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1SKW" version="SP_153"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1SL0" version="SP_153"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1SL1" version="SP_153"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1SL2" version="SP_153"/>
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          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6YEV" version="SP_153"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-31856N"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
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            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="83333">
          <names>
            <shortLabel>ecoli</shortLabel>
            <fullName>Escherichia coli (strain K12)</fullName>
          </names>
        </organism>
        <sequence>MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein P0AA25 original sequence version: 67</attribute>
          <attribute name="crc64">EF5933EA29668EE9</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4" imexId="IM-27304-1">
        <names>
          <shortLabel>trxa-trxb-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="rcsb pdb" dbAc="MI:0460" id="1F6M" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1029844" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-27304-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <featureList>
              <feature id="6">
                <names>
                  <shortLabel>region</shortLabel>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-1029849" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>sufficient to bind</shortLabel>
                    <fullName>sufficient binding region</fullName>
                    <alias type="synonym" typeAc="MI:1041">sufficient to bind</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0442" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608899" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="2"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="321"/>
                  </featureRange>
                </featureRangeList>
              </feature>
            </featureList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="83333">
                <names>
                  <shortLabel>ecoli</shortLabel>
                  <fullName>Escherichia coli (strain K12)</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
            <stoichiometry value="4"/>
          </participant>
          <participant id="7">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <featureList>
              <feature id="8">
                <names>
                  <shortLabel>region</shortLabel>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-1029846" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>sufficient to bind</shortLabel>
                    <fullName>sufficient binding region</fullName>
                    <alias type="synonym" typeAc="MI:1041">sufficient to bind</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0442" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608899" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="2"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="109"/>
                  </featureRange>
                </featureRangeList>
              </feature>
            </featureList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="83333">
                <names>
                  <shortLabel>ecoli</shortLabel>
                  <fullName>Escherichia coli (strain K12)</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
            <stoichiometry value="4"/>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="assembly">octameric, multimeric</attribute>
          <attribute name="3d-r-factors" nameAc="MI:0631">working 79.500%, free 75.300%</attribute>
          <attribute name="3d-resolution" nameAc="MI:0632">2.95A</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>