UniProt UniProt http://www.uniprot.org PILRalpha, a novel immunoreceptor tyrosine-based inhibitory motif-bearing protein, recruits SHP-1 upon tyrosine phosphorylation and is paired with the truncated counterpart PILRbeta. PILRalpha, a novel immunoreceptor tyrosine-based inhibitory motif-bearing protein, recruits SHP-1 upon tyrosine phosphorylation and is paired with the truncated counterpart PILRbeta. J. Biol. Chem. (0021-9258) 2000 imex curation Mousseau DD., Banville D., L'Abbe D., Bouchard P., Shen SH. shi.shen@nrc.ca 25-OCT-2006: Contacted by JYOTI. Human mammary gland cDNA Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Mousseau DD., Banville D., L'Abbe D., Bouchard P., Shen SH. shi.shen@nrc.ca Accepted 2006-JUL-17 by DAVET. 25-OCT-2006: Contacted by JYOTI. Human mammary gland cDNA J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation PILRalpha, a novel immunoreceptor tyrosine-based inhibitory motif-bearing protein, recruits SHP-1 upon tyrosine phosphorylation and is paired with the truncated counterpart PILRbeta. PILRalpha, a novel immunoreceptor tyrosine-based inhibitory motif-bearing protein, recruits SHP-1 upon tyrosine phosphorylation and is paired with the truncated counterpart PILRbeta. J. Biol. Chem. (0021-9258) 2000 imex curation Mousseau DD., Banville D., L'Abbe D., Bouchard P., Shen SH. shi.shen@nrc.ca 25-OCT-2006: Contacted by JYOTI. Human mammary gland cDNA Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Mousseau DD., Banville D., L'Abbe D., Bouchard P., Shen SH. Human 293T cells have beem transfected with PILRA and PTPN6, treated or non-treated with pervanadate. shi.shen@nrc.ca Accepted 2006-JUL-17 by DAVET. 25-OCT-2006: Contacted by JYOTI. Human mammary gland cDNA J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation PILRalpha, a novel immunoreceptor tyrosine-based inhibitory motif-bearing protein, recruits SHP-1 upon tyrosine phosphorylation and is paired with the truncated counterpart PILRbeta. PILRalpha, a novel immunoreceptor tyrosine-based inhibitory motif-bearing protein, recruits SHP-1 upon tyrosine phosphorylation and is paired with the truncated counterpart PILRbeta. J. Biol. Chem. (0021-9258) 2000 imex curation Mousseau DD., Banville D., L'Abbe D., Bouchard P., Shen SH. shi.shen@nrc.ca 25-OCT-2006: Contacted by JYOTI. Human mammary gland cDNA Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Mousseau DD., Banville D., L'Abbe D., Bouchard P., Shen SH. synthetic tyrosine-phosphorylated peptides corresponding to PILRA sequences have been used. shi.shen@nrc.ca Accepted 2006-JUL-17 by DAVET. 25-OCT-2006: Contacted by JYOTI. Human mammary gland cDNA J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation pilra_human Paired immunoglobulin-like type 2 receptor alpha PILRA Inhibitory receptor PILR-alpha Cell surface receptor FDF03 protein protein human Homo sapiens Human MGRPLLLPLLPLLLPPAFLQPSGSTGSGPSYLYGVTQPKHLSASMGGSVEIPFSFYYPWELATAPDVRISWRRGHFHRQSFYSTRPPSIHKDYVNRLFLNWTEGQKSGFLRISNLQKQDQSVYFCRVELDTRSSGRQQWQSIEGTKLSITQAVTTTTQRPSSMTTTWRLSSTTTTTGLRVTQGKRRSDSWHISLETAVGVAVAVTVLGIMILGLICLLRWRRRKGQQRTKATTPAREPFQNTEEPYENIRNEGQNTDPKLNPKDDGIVYASLALSSSTSPRAPPSHRPLKSPQNETLYSVLKA 77BD068C63274A40 shps1_human Tyrosine-protein phosphatase non-receptor type substrate 1 Inhibitory receptor SHPS-1 Signal-regulatory protein alpha-1 Signal-regulatory protein alpha-2 Signal-regulatory protein alpha-3 MyD-1 antigen Brain Ig-like molecule with tyrosine-based activation motifs Macrophage fusion receptor p84 CD172 antigen-like family member A SIRPA BIT MFR MYD1 SHPS1 SIRP PTPNS1 protein protein human Homo sapiens Human MEPAGPAPGRLGPLLCLLLAASCAWSGVAGEEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNERNIYIVVGVVCTLLVALLMAALYLVRIRQKKAQGSTSSTRLHEPEKNAREITQDTNDITYADLNLPKGKKPAPQAAEPNNHTEYASIQTSPQPASEDTLTYADLDMVHLNRTPKQPAPKPEPSFSEYASVQVPRK 18D2FD04F6182AD0 grb2_human Growth factor receptor-bound protein 2 GRB2 ASH Adapter protein GRB2 SH2/SH3 adapter GRB2 Protein Ash protein protein human Homo sapiens Human MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV 83A4B0BA1B248DC4 lair1_human Leukocyte-associated immunoglobulin-like receptor 1 LAIR1 CD305 protein protein human Homo sapiens Human MSPHPTALLGLVLCLAQTIHTQEEDLPRPSISAEPGTVIPLGSHVTFVCRGPVGVQTFRLERESRSTYNDTEDVSQASPSESEARFRIDSVSEGNAGPYRCIYYKPPKWSEQSDYLELLVKETSGGPDSPDTEPGSSAGPTQRPSDNSHNEHAPASQGLKAEHLYILIGVSVVFLFCLLLLVLFCLHRQNQIKQGPPRSKDEEQKPQQRPDLAVDVLERTADKATVNGLPEKDRETDTSALAAGSSQEVTYAQLDHWALTQRTARAVSPQSTKPMAESITYAAVARH 752EFB2FCC29B8E8 ptn6_human Tyrosine-protein phosphatase non-receptor type 6 PTPN6 HCP Protein-tyrosine phosphatase 1C Hematopoietic cell protein-tyrosine phosphatase SH-PTP1 Protein-tyrosine phosphatase SHP-1 PTP1C protein protein human Homo sapiens Human MVRWFHRDLSGLDAETLLKGRGVHGSFLARPSRKNQGDFSLSVRVGDQVTHIRIQNSGDFYDLYGGEKFATLTELVEYYTQQQGVLQDRDGTIIHLKYPLNCSDPTSERWYHGHMSGGQAETLLQAKGEPWTFLVRESLSQPGDFVLSVLSDQPKAGPGSPLRVTHIKVMCEGGRYTVGGLETFDSLTDLVEHFKKTGIEEASGAFVYLRQPYYATRVNAADIENRVLELNKKQESEDTAKAGFWEEFESLQKQEVKNLHQRLEGQRPENKGKNRYKNILPFDHSRVILQGRDSNIPGSDYINANYIKNQLLGPDENAKTYIASQGCLEATVNDFWQMAWQENSRVIVMTTREVEKGRNKCVPYWPEVGMQRAYGPYSVTNCGEHDTTEYKLRTLQVSPLDNGDLIREIWHYQYLSWPDHGVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMENISTKGLDCDIDIQKTIQMVRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEVLQSQKGQESEYGNITYPPAMKNAHAKASRTSSKHKEDVYENLHTKNKREEKVKKQRSADKEKSKGSLKRK DIP protein P29350 original sequence version: 183 DIP protein P29350 original sequence version: 139 DIP protein P29350-1 original sequence version: 139 4D7736C21D3542D2 ptpn6-pilra-1 1 4 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 8 unspecified role unspecified role bait bait P29350:p.Cys453Ser catalytic mutant mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 16:36:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys453ser' Tue Jun 30 18:23:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys453Ser' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association 1 ptpn6-lair1-1 1 7 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 8 unspecified role unspecified role bait bait P29350:p.Cys453Ser catalytic mutant mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 16:09:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys453ser' Tue Jun 30 17:28:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys453Ser' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association 1 ptpn6-grb2-1 1 8 unspecified role unspecified role bait bait P29350:p.Cys453Ser catalytic mutant mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 18:19:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys453ser' Tue Jun 30 19:50:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys453Ser' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 6 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association 1 ptpn6-sirpa-1 1 5 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 8 unspecified role unspecified role bait bait P29350:p.Cys453Ser catalytic mutant mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 14:24:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys453ser' Tue Jun 30 19:19:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys453Ser' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association 1 pilra-ptpn6-1 2 4 unspecified role unspecified role bait bait region phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9UKJ1:p.Tyr269Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 16:29:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr269phe' Tue Jun 30 17:34:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr269Phe' Q9UKJ1:p.[Tyr269Phe;Tyr298Phe] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F Fri Jul 22 11:04:03 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr298phe,tyr269phe' Wed May 02 16:20:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr269phe,tyr298phe' Tue Jun 30 17:07:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr269Phe;Tyr298Phe]' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 8 unspecified role unspecified role prey prey P29350:p.Asp419Ala substrate trapping mutatnt mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain D A Wed May 02 17:25:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp419ala' Tue Jun 30 19:22:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp419Ala' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 3A and B, and 5 Phosphorylation of PPR1A is required for the interaction. ptpn6-pilra-2 3 4 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain tyr269 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh2-domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Saturation kD was determined using 400 nM of peptides. Kinetic Kd: 47.8E-9 M, with Kon:5.10E4 M-1s-1 and Koff:2.48E3 M-1s-1; 0.5 to 2000 nM have been used to determine kinetic Kd. 6 ptpn6-pilra-3 3 4 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain tyr298 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role prey prey sh2-domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Saturation kD was determined using 400 nM of peptides. Kinetic Kd: 3000E-9 M, with Kon:0.46E4 M-1s-1 and Koff:12.9E3 M-1s-1; 0.5 to 2000 nM have been used to determine kinetic Kd. 6 ptpn6-pilra-4b 3 4 unspecified role unspecified role bait bait tyr269 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh2-domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Saturation kD was determined using 400 nM of peptides. Kinetic Kd: 214E-9 M, with Kon:2.42E4 M-1s-1 and Koff:3.78E3 M-1s-1; 0.5 to 2000 nM have been used to determine kinetic Kd. 6